A human skin model to evaluate the protective effect of compounds against UVA damage
Objective The main function of skin is to protect the body from external aggressions. Over time, normal skin ageing is accelerated by external stresses such as smoking, pollution, chemical products and radiation. UV light, in particular UVA, causes DNA damage, apoptosis and morphological modificatio...
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| Veröffentlicht in: | International journal of cosmetic science 2019-12, Vol.41 (6), p.594-603 |
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| creator | Abadie, S. Bedos, P. Rouquette, J. |
| description | Objective
The main function of skin is to protect the body from external aggressions. Over time, normal skin ageing is accelerated by external stresses such as smoking, pollution, chemical products and radiation. UV light, in particular UVA, causes DNA damage, apoptosis and morphological modifications, which are responsible for both premature ageing and cancer. The aim of this study was to establish a discriminatory and sufficiently reproducible cutaneous model for evaluating UVA damage, to enable testing for effectiveness of potentially protective compounds.
Methods
The cutaneous model is based on Human skin explants irradiated with UVA. Deleterious effects on epidermis were observed and quantified by haematoxylin–eosin staining and by immunofluorescence of ɣ‐H2Ax, cytokeratin 5, involucrin and loricrin protein. Dermis deterioration was evaluated by transmission electronic microscopy and zymography in situ.
Results
We were able to observe and quantify deleterious effects associated with UVA irradiation: epidermal and dermal disruption, appearance of Sunburn cells, increased DNA damage and induced apoptosis. The use of this model in the evaluation of protective compounds was first confirmed using sunscreens, then further validated with a panel of active ingredients which showed beneficial effects on epidermis morphology and DNA integrity after UVA exposure.
Conclusion
We have developed a model and a standardized protocol, based on the use of human skin explants, which allows us to explore the protective effect of active ingredients to environmental stresses such as UVA.
Résumé
Objectifs
La fonction principale de la peau est de protéger le corps des agressions externes. Au cours du temps, le vieillissement naturel de la peau est accéléré par des stress externes comme la cigarette, la pollution, les produits chimiques et les radiations solaires. Le rayonnement ultraviolet, en particulier les UVA, cause des dommages de l'ADN, l'apoptose et des modifications morphologiques qui sont responsables du vieillissement prématuré et de cancers. Le but de cette étude est d'établir un modèle cutané reproductible et discriminatoire pour évaluer les dommages créé par les UVA et tester l'efficacité de potentiels produits protecteurs.
Méthodes
Ce modèle cutané est basé sur un explant de peau humaine irradié aux UVA. Les effets délétères sur l'épiderme sont observés et quantifiés par coloration Hématoxyline‐éosine et par des immunofluorescence des protéines ɣ‐H2Ax, cytokérat |
| doi_str_mv | 10.1111/ics.12579 |
| format | Article |
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The main function of skin is to protect the body from external aggressions. Over time, normal skin ageing is accelerated by external stresses such as smoking, pollution, chemical products and radiation. UV light, in particular UVA, causes DNA damage, apoptosis and morphological modifications, which are responsible for both premature ageing and cancer. The aim of this study was to establish a discriminatory and sufficiently reproducible cutaneous model for evaluating UVA damage, to enable testing for effectiveness of potentially protective compounds.
Methods
The cutaneous model is based on Human skin explants irradiated with UVA. Deleterious effects on epidermis were observed and quantified by haematoxylin–eosin staining and by immunofluorescence of ɣ‐H2Ax, cytokeratin 5, involucrin and loricrin protein. Dermis deterioration was evaluated by transmission electronic microscopy and zymography in situ.
Results
We were able to observe and quantify deleterious effects associated with UVA irradiation: epidermal and dermal disruption, appearance of Sunburn cells, increased DNA damage and induced apoptosis. The use of this model in the evaluation of protective compounds was first confirmed using sunscreens, then further validated with a panel of active ingredients which showed beneficial effects on epidermis morphology and DNA integrity after UVA exposure.
Conclusion
We have developed a model and a standardized protocol, based on the use of human skin explants, which allows us to explore the protective effect of active ingredients to environmental stresses such as UVA.
Résumé
Objectifs
La fonction principale de la peau est de protéger le corps des agressions externes. Au cours du temps, le vieillissement naturel de la peau est accéléré par des stress externes comme la cigarette, la pollution, les produits chimiques et les radiations solaires. Le rayonnement ultraviolet, en particulier les UVA, cause des dommages de l'ADN, l'apoptose et des modifications morphologiques qui sont responsables du vieillissement prématuré et de cancers. Le but de cette étude est d'établir un modèle cutané reproductible et discriminatoire pour évaluer les dommages créé par les UVA et tester l'efficacité de potentiels produits protecteurs.
Méthodes
Ce modèle cutané est basé sur un explant de peau humaine irradié aux UVA. Les effets délétères sur l'épiderme sont observés et quantifiés par coloration Hématoxyline‐éosine et par des immunofluorescence des protéines ɣ‐H2Ax, cytokératine 5, involucrine et loricrine. Le détérioration du derme est évaluée par microscopie électronique à transmission et par zymographie in situ.
Résultats
Nous avons observé et quantifié des effets délétères associés aux irradiation UVA: détérioration de l'épiderme et du derme, apparition de cellules "coup de soleil", augmentation des dommages de l'ADN et l'induction de l'apoptose. L'utilisation du modèle pour tester des nouveaux composés a été premièrement validée avec l'utilisation d'un filtre solaire puis validée par le test d'un panel d'ingrédients actifs qui ont montré des effets bénéfiques sur la morphologie de l'épiderme et l'intégrité de l'ADN après exposition aux UVA.
Conclusion
Nous avons développé un modèle et un protocole standardisé basé sur l'utilisation d'un explant de peau humaine qui permet d'explorer l'effet protecteur ingrédients actifs contre des stress environnementaux comme les UVA.
(a) Representative images of H&E staining acquired from sections of paraffin‐embedded human skin explants: control (foil protected), post UVA irradiation, or UVA irradiation with sunscreen (X40, scale bars 100μm, *stratum corneum, **epidermis, ***dermis). (b) Percentage of sunburn cells. ***P‐value < 0.001. Observation on 10 skin donors for control and irradiated skin, and 5 donors for irradiated with sunscreen skin.</description><identifier>ISSN: 0142-5463</identifier><identifier>EISSN: 1468-2494</identifier><identifier>DOI: 10.1111/ics.12579</identifier><identifier>PMID: 31512246</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Adult ; Aged ; Aging ; Apoptosis ; Apoptosis - radiation effects ; Chemical pollution ; Coloration ; Cytokeratin ; Damage assessment ; Deoxyribonucleic acid ; Dermis ; Disruption ; DNA ; DNA Breaks, Double-Stranded - radiation effects ; DNA damage ; Environmental effects ; Environmental stress ; Epidermis ; Explants ; Female ; Humans ; Immunofluorescence ; Ingredients ; Irradiation ; Keratinocytes - radiation effects ; Middle Aged ; Models, Biological ; Morphology ; Organic chemistry ; Pollution ; Radiation damage ; safety testing ; Skin ; Skin - drug effects ; Skin - radiation effects ; skin barrier ; skin explant ; skin physiology/structure ; Smoking ; Stresses ; Sun screens ; Sunburn ; Sunscreening Agents - pharmacology ; Sunscreens ; Ultraviolet radiation ; Ultraviolet Rays ; UVA</subject><ispartof>International journal of cosmetic science, 2019-12, Vol.41 (6), p.594-603</ispartof><rights>2019 Society of Cosmetic Scientists and the Société Française de Cosmétologie</rights><rights>2019 Society of Cosmetic Scientists and the Société Française de Cosmétologie.</rights><rights>Copyright © 2019 Society of Cosmetic Scientists and the Société Française de Cosmétologie</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-a682f6bea185863a3d0a911dbc0d8e27a799d177aaac9ea52e1bf730d3fb5e873</citedby><cites>FETCH-LOGICAL-c3539-a682f6bea185863a3d0a911dbc0d8e27a799d177aaac9ea52e1bf730d3fb5e873</cites><orcidid>0000-0001-5028-7079</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fics.12579$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fics.12579$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31512246$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abadie, S.</creatorcontrib><creatorcontrib>Bedos, P.</creatorcontrib><creatorcontrib>Rouquette, J.</creatorcontrib><title>A human skin model to evaluate the protective effect of compounds against UVA damage</title><title>International journal of cosmetic science</title><addtitle>Int J Cosmet Sci</addtitle><description>Objective
The main function of skin is to protect the body from external aggressions. Over time, normal skin ageing is accelerated by external stresses such as smoking, pollution, chemical products and radiation. UV light, in particular UVA, causes DNA damage, apoptosis and morphological modifications, which are responsible for both premature ageing and cancer. The aim of this study was to establish a discriminatory and sufficiently reproducible cutaneous model for evaluating UVA damage, to enable testing for effectiveness of potentially protective compounds.
Methods
The cutaneous model is based on Human skin explants irradiated with UVA. Deleterious effects on epidermis were observed and quantified by haematoxylin–eosin staining and by immunofluorescence of ɣ‐H2Ax, cytokeratin 5, involucrin and loricrin protein. Dermis deterioration was evaluated by transmission electronic microscopy and zymography in situ.
Results
We were able to observe and quantify deleterious effects associated with UVA irradiation: epidermal and dermal disruption, appearance of Sunburn cells, increased DNA damage and induced apoptosis. The use of this model in the evaluation of protective compounds was first confirmed using sunscreens, then further validated with a panel of active ingredients which showed beneficial effects on epidermis morphology and DNA integrity after UVA exposure.
Conclusion
We have developed a model and a standardized protocol, based on the use of human skin explants, which allows us to explore the protective effect of active ingredients to environmental stresses such as UVA.
Résumé
Objectifs
La fonction principale de la peau est de protéger le corps des agressions externes. Au cours du temps, le vieillissement naturel de la peau est accéléré par des stress externes comme la cigarette, la pollution, les produits chimiques et les radiations solaires. Le rayonnement ultraviolet, en particulier les UVA, cause des dommages de l'ADN, l'apoptose et des modifications morphologiques qui sont responsables du vieillissement prématuré et de cancers. Le but de cette étude est d'établir un modèle cutané reproductible et discriminatoire pour évaluer les dommages créé par les UVA et tester l'efficacité de potentiels produits protecteurs.
Méthodes
Ce modèle cutané est basé sur un explant de peau humaine irradié aux UVA. Les effets délétères sur l'épiderme sont observés et quantifiés par coloration Hématoxyline‐éosine et par des immunofluorescence des protéines ɣ‐H2Ax, cytokératine 5, involucrine et loricrine. Le détérioration du derme est évaluée par microscopie électronique à transmission et par zymographie in situ.
Résultats
Nous avons observé et quantifié des effets délétères associés aux irradiation UVA: détérioration de l'épiderme et du derme, apparition de cellules "coup de soleil", augmentation des dommages de l'ADN et l'induction de l'apoptose. L'utilisation du modèle pour tester des nouveaux composés a été premièrement validée avec l'utilisation d'un filtre solaire puis validée par le test d'un panel d'ingrédients actifs qui ont montré des effets bénéfiques sur la morphologie de l'épiderme et l'intégrité de l'ADN après exposition aux UVA.
Conclusion
Nous avons développé un modèle et un protocole standardisé basé sur l'utilisation d'un explant de peau humaine qui permet d'explorer l'effet protecteur ingrédients actifs contre des stress environnementaux comme les UVA.
(a) Representative images of H&E staining acquired from sections of paraffin‐embedded human skin explants: control (foil protected), post UVA irradiation, or UVA irradiation with sunscreen (X40, scale bars 100μm, *stratum corneum, **epidermis, ***dermis). (b) Percentage of sunburn cells. ***P‐value < 0.001. Observation on 10 skin donors for control and irradiated skin, and 5 donors for irradiated with sunscreen skin.</description><subject>Adult</subject><subject>Aged</subject><subject>Aging</subject><subject>Apoptosis</subject><subject>Apoptosis - radiation effects</subject><subject>Chemical pollution</subject><subject>Coloration</subject><subject>Cytokeratin</subject><subject>Damage assessment</subject><subject>Deoxyribonucleic acid</subject><subject>Dermis</subject><subject>Disruption</subject><subject>DNA</subject><subject>DNA Breaks, Double-Stranded - radiation effects</subject><subject>DNA damage</subject><subject>Environmental effects</subject><subject>Environmental stress</subject><subject>Epidermis</subject><subject>Explants</subject><subject>Female</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Ingredients</subject><subject>Irradiation</subject><subject>Keratinocytes - radiation effects</subject><subject>Middle Aged</subject><subject>Models, Biological</subject><subject>Morphology</subject><subject>Organic chemistry</subject><subject>Pollution</subject><subject>Radiation damage</subject><subject>safety testing</subject><subject>Skin</subject><subject>Skin - drug effects</subject><subject>Skin - radiation effects</subject><subject>skin barrier</subject><subject>skin explant</subject><subject>skin physiology/structure</subject><subject>Smoking</subject><subject>Stresses</subject><subject>Sun screens</subject><subject>Sunburn</subject><subject>Sunscreening Agents - pharmacology</subject><subject>Sunscreens</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><subject>UVA</subject><issn>0142-5463</issn><issn>1468-2494</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtPwkAQxzdGI4ge_AJmE08eCvvoY_dIiA8SEg-C12banYViH9htMXx7K0VvzmXm8Mt_Zn6E3HI25l1NstSNuQgifUaG3A-VJ3ztn5Mh477wAj-UA3Ll3JYx5mslL8lA8oAL4YdDspzSTVtASd1HVtKiMpjTpqK4h7yFBmmzQbqrqwbTJtsjRWu7iVaWplWxq9rSOApryErX0NX7lBooYI3X5MJC7vDm1Edk9fS4nL14i9fn-Wy68FIZSO1BqIQNEwSuAhVKkIaB5twkKTMKRQSR1oZHEQCkGiEQyBMbSWakTQJUkRyR-z63u_CzRdfE26qty25lLLoXdajkkXroqbSunKvRxrs6K6A-xJzFP_7izl989Nexd6fENinQ_JG_wjpg0gNfWY6H_5Pi-eytj_wGt_V5ew</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Abadie, S.</creator><creator>Bedos, P.</creator><creator>Rouquette, J.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0001-5028-7079</orcidid></search><sort><creationdate>201912</creationdate><title>A human skin model to evaluate the protective effect of compounds against UVA damage</title><author>Abadie, S. ; Bedos, P. ; Rouquette, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-a682f6bea185863a3d0a911dbc0d8e27a799d177aaac9ea52e1bf730d3fb5e873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aging</topic><topic>Apoptosis</topic><topic>Apoptosis - radiation effects</topic><topic>Chemical pollution</topic><topic>Coloration</topic><topic>Cytokeratin</topic><topic>Damage assessment</topic><topic>Deoxyribonucleic acid</topic><topic>Dermis</topic><topic>Disruption</topic><topic>DNA</topic><topic>DNA Breaks, Double-Stranded - radiation effects</topic><topic>DNA damage</topic><topic>Environmental effects</topic><topic>Environmental stress</topic><topic>Epidermis</topic><topic>Explants</topic><topic>Female</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Ingredients</topic><topic>Irradiation</topic><topic>Keratinocytes - radiation effects</topic><topic>Middle Aged</topic><topic>Models, Biological</topic><topic>Morphology</topic><topic>Organic chemistry</topic><topic>Pollution</topic><topic>Radiation damage</topic><topic>safety testing</topic><topic>Skin</topic><topic>Skin - drug effects</topic><topic>Skin - radiation effects</topic><topic>skin barrier</topic><topic>skin explant</topic><topic>skin physiology/structure</topic><topic>Smoking</topic><topic>Stresses</topic><topic>Sun screens</topic><topic>Sunburn</topic><topic>Sunscreening Agents - pharmacology</topic><topic>Sunscreens</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><topic>UVA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abadie, S.</creatorcontrib><creatorcontrib>Bedos, P.</creatorcontrib><creatorcontrib>Rouquette, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of cosmetic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abadie, S.</au><au>Bedos, P.</au><au>Rouquette, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A human skin model to evaluate the protective effect of compounds against UVA damage</atitle><jtitle>International journal of cosmetic science</jtitle><addtitle>Int J Cosmet Sci</addtitle><date>2019-12</date><risdate>2019</risdate><volume>41</volume><issue>6</issue><spage>594</spage><epage>603</epage><pages>594-603</pages><issn>0142-5463</issn><eissn>1468-2494</eissn><abstract>Objective
The main function of skin is to protect the body from external aggressions. Over time, normal skin ageing is accelerated by external stresses such as smoking, pollution, chemical products and radiation. UV light, in particular UVA, causes DNA damage, apoptosis and morphological modifications, which are responsible for both premature ageing and cancer. The aim of this study was to establish a discriminatory and sufficiently reproducible cutaneous model for evaluating UVA damage, to enable testing for effectiveness of potentially protective compounds.
Methods
The cutaneous model is based on Human skin explants irradiated with UVA. Deleterious effects on epidermis were observed and quantified by haematoxylin–eosin staining and by immunofluorescence of ɣ‐H2Ax, cytokeratin 5, involucrin and loricrin protein. Dermis deterioration was evaluated by transmission electronic microscopy and zymography in situ.
Results
We were able to observe and quantify deleterious effects associated with UVA irradiation: epidermal and dermal disruption, appearance of Sunburn cells, increased DNA damage and induced apoptosis. The use of this model in the evaluation of protective compounds was first confirmed using sunscreens, then further validated with a panel of active ingredients which showed beneficial effects on epidermis morphology and DNA integrity after UVA exposure.
Conclusion
We have developed a model and a standardized protocol, based on the use of human skin explants, which allows us to explore the protective effect of active ingredients to environmental stresses such as UVA.
Résumé
Objectifs
La fonction principale de la peau est de protéger le corps des agressions externes. Au cours du temps, le vieillissement naturel de la peau est accéléré par des stress externes comme la cigarette, la pollution, les produits chimiques et les radiations solaires. Le rayonnement ultraviolet, en particulier les UVA, cause des dommages de l'ADN, l'apoptose et des modifications morphologiques qui sont responsables du vieillissement prématuré et de cancers. Le but de cette étude est d'établir un modèle cutané reproductible et discriminatoire pour évaluer les dommages créé par les UVA et tester l'efficacité de potentiels produits protecteurs.
Méthodes
Ce modèle cutané est basé sur un explant de peau humaine irradié aux UVA. Les effets délétères sur l'épiderme sont observés et quantifiés par coloration Hématoxyline‐éosine et par des immunofluorescence des protéines ɣ‐H2Ax, cytokératine 5, involucrine et loricrine. Le détérioration du derme est évaluée par microscopie électronique à transmission et par zymographie in situ.
Résultats
Nous avons observé et quantifié des effets délétères associés aux irradiation UVA: détérioration de l'épiderme et du derme, apparition de cellules "coup de soleil", augmentation des dommages de l'ADN et l'induction de l'apoptose. L'utilisation du modèle pour tester des nouveaux composés a été premièrement validée avec l'utilisation d'un filtre solaire puis validée par le test d'un panel d'ingrédients actifs qui ont montré des effets bénéfiques sur la morphologie de l'épiderme et l'intégrité de l'ADN après exposition aux UVA.
Conclusion
Nous avons développé un modèle et un protocole standardisé basé sur l'utilisation d'un explant de peau humaine qui permet d'explorer l'effet protecteur ingrédients actifs contre des stress environnementaux comme les UVA.
(a) Representative images of H&E staining acquired from sections of paraffin‐embedded human skin explants: control (foil protected), post UVA irradiation, or UVA irradiation with sunscreen (X40, scale bars 100μm, *stratum corneum, **epidermis, ***dermis). (b) Percentage of sunburn cells. ***P‐value < 0.001. Observation on 10 skin donors for control and irradiated skin, and 5 donors for irradiated with sunscreen skin.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31512246</pmid><doi>10.1111/ics.12579</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5028-7079</orcidid></addata></record> |
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| ispartof | International journal of cosmetic science, 2019-12, Vol.41 (6), p.594-603 |
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| subjects | Adult Aged Aging Apoptosis Apoptosis - radiation effects Chemical pollution Coloration Cytokeratin Damage assessment Deoxyribonucleic acid Dermis Disruption DNA DNA Breaks, Double-Stranded - radiation effects DNA damage Environmental effects Environmental stress Epidermis Explants Female Humans Immunofluorescence Ingredients Irradiation Keratinocytes - radiation effects Middle Aged Models, Biological Morphology Organic chemistry Pollution Radiation damage safety testing Skin Skin - drug effects Skin - radiation effects skin barrier skin explant skin physiology/structure Smoking Stresses Sun screens Sunburn Sunscreening Agents - pharmacology Sunscreens Ultraviolet radiation Ultraviolet Rays UVA |
| title | A human skin model to evaluate the protective effect of compounds against UVA damage |
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