Molecular Screening, Isolation, and Characterization of Enterohemorrhagic Escherichia coli O157:H7 from Retail Shrimp
Foodborne outbreaks attributed to the contamination of foods with enterohemorrhagic Escherichia coli (EHEC) O157:H7 are a growing global concern. Fish and shrimp samples obtained from different retail fish markets in Cochin, India, were screened by direct PCR assays targeting three important virulen...
Gespeichert in:
| Veröffentlicht in: | Journal of food protection 2010, Vol.73 (1), p.97-103 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 103 |
|---|---|
| container_issue | 1 |
| container_start_page | 97 |
| container_title | Journal of food protection |
| container_volume | 73 |
| creator | Surendraraj, Alagarsamy Thampuran, Nirmala Joseph, Toms C |
| description | Foodborne outbreaks attributed to the contamination of foods with enterohemorrhagic Escherichia coli (EHEC) O157:H7 are a growing global concern. Fish and shrimp samples obtained from different retail fish markets in Cochin, India, were screened by direct PCR assays targeting three important virulence markers of EHEC, the intimin protein (eaeA gene), enterohemolysin (hlyA gene), and Shiga toxin (stx gene). One shrimp (Fenneropenaeus indicus) sample was positive for all these virulence markers, and seven typical E. coli O157:H7 isolates were recovered from the marker-positive shrimp sample. This is the first report of recovery of typical E. coli O157:H7 from fish or shellfish in India. All the typical EHEC isolates had a characteristic reaction in eosin methylene blue agar and belonged to IMViC (indole, methyl red, Voges Proskauer, Simmons citrate reactions) biotype I. These isolates also were negative for sorbitol and methylumbelliferyl-β-glucuronide and exhibited β-hemolytic activity. One isolate showed self-agglutination for E. coli O157 antisera and produced a false-positive reaction with CHROMagar O157. These typical EHEC isolates belonged to a restricted biotype group and had a very low multiple antibiotic resistance index. Isolation of E. coli O157:H7 in fish and shellfish indicates that strict adherence to hygienic handling methods and proper cooking or processing is needed before consumption of these products. |
| doi_str_mv | 10.4315/0362-028X-73.1.97 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_231327361</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1952074881</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-797e7d0be0e8e21a46df694ab0d922eeacc5f1c6dd3ac94af3411f153e70a5ac3</originalsourceid><addsrcrecordid>eNpF0FFv0zAQB3BrGtrK4APsZbOQeFuKz07ieG-oKmzS0CTKJN6sq2M3ntK4s5MH-PS4tIwny-ff3Vl_Qi6BzUsB1Scmal4w3vwspJjDXMkTMgNVloViSp6S2ev7OXmb0jNjjCten5FzzlgFHGBGpm-ht2bqMdKVidYOftjc0PsUehx9GG4oDi1ddBjRjDb633-rNDi6HPI9dHYbYuxw4w1dJtNlYjqP1ITe00eo5O2dpC6GLf1uR_Q9XXXRb3fvyBuHfbLvj-cFefqy_LG4Kx4ev94vPj8URig-FlJJK1u2tsw2lgOWdetqVeKatYpza9GYyoGp21agyXUnSgAHlbCSYYVGXJAPh7m7GF4mm0b9HKY45JWaCxBcihoyggMyMaQUrdO7_EeMvzQwvc9Z73PU-xy1FBq0krnn6jh4Wm9t-9rxL9gMPh4BJoO9izgYn_47LkQtoczu-uAcBo2bmM3TijMQDKRqGtaIPwiJj3U</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>231327361</pqid></control><display><type>article</type><title>Molecular Screening, Isolation, and Characterization of Enterohemorrhagic Escherichia coli O157:H7 from Retail Shrimp</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Surendraraj, Alagarsamy ; Thampuran, Nirmala ; Joseph, Toms C</creator><creatorcontrib>Surendraraj, Alagarsamy ; Thampuran, Nirmala ; Joseph, Toms C</creatorcontrib><description>Foodborne outbreaks attributed to the contamination of foods with enterohemorrhagic Escherichia coli (EHEC) O157:H7 are a growing global concern. Fish and shrimp samples obtained from different retail fish markets in Cochin, India, were screened by direct PCR assays targeting three important virulence markers of EHEC, the intimin protein (eaeA gene), enterohemolysin (hlyA gene), and Shiga toxin (stx gene). One shrimp (Fenneropenaeus indicus) sample was positive for all these virulence markers, and seven typical E. coli O157:H7 isolates were recovered from the marker-positive shrimp sample. This is the first report of recovery of typical E. coli O157:H7 from fish or shellfish in India. All the typical EHEC isolates had a characteristic reaction in eosin methylene blue agar and belonged to IMViC (indole, methyl red, Voges Proskauer, Simmons citrate reactions) biotype I. These isolates also were negative for sorbitol and methylumbelliferyl-β-glucuronide and exhibited β-hemolytic activity. One isolate showed self-agglutination for E. coli O157 antisera and produced a false-positive reaction with CHROMagar O157. These typical EHEC isolates belonged to a restricted biotype group and had a very low multiple antibiotic resistance index. Isolation of E. coli O157:H7 in fish and shellfish indicates that strict adherence to hygienic handling methods and proper cooking or processing is needed before consumption of these products.</description><identifier>ISSN: 0362-028X</identifier><identifier>EISSN: 1944-9097</identifier><identifier>DOI: 10.4315/0362-028X-73.1.97</identifier><identifier>PMID: 20051211</identifier><identifier>CODEN: JFPRDR</identifier><language>eng</language><publisher>Des Moines, IA: International Association for Food Protection</publisher><subject>Animals ; Antibiotic resistance ; bacterial contamination ; Biological and medical sciences ; biotypes ; Colony Count, Microbial ; Consumer Product Safety ; E coli ; enrichment culture ; enterohemorrhagic Escherichia coli ; Enzymes ; Epidemics ; Escherichia coli O157 - classification ; Escherichia coli O157 - genetics ; Escherichia coli O157 - isolation & purification ; Escherichia coli O157:H7 ; Fenneropenaeus indicus ; Fish ; Fish and seafood industries ; Food contamination & poisoning ; Food Contamination - analysis ; Food Handling - methods ; Food industries ; Food Microbiology ; food pathogens ; Food safety ; Fundamental and applied biological sciences. Psychology ; Genes ; hemolysis ; Humans ; India ; isolation ; microbial detection ; Molecular biology ; molecular systematics ; Penaeidae - microbiology ; Polymerase Chain Reaction ; raw fish ; retail marketing ; ribotypes ; seafoods ; serotypes ; Shellfish ; Shellfish - microbiology ; shrimp ; Toxins ; Virulence ; Virulence Factors - analysis ; Virulence Factors - genetics ; virulence genes</subject><ispartof>Journal of food protection, 2010, Vol.73 (1), p.97-103</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright International Association for Food Protection Jan 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-797e7d0be0e8e21a46df694ab0d922eeacc5f1c6dd3ac94af3411f153e70a5ac3</citedby><cites>FETCH-LOGICAL-c392t-797e7d0be0e8e21a46df694ab0d922eeacc5f1c6dd3ac94af3411f153e70a5ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22336714$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20051211$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Surendraraj, Alagarsamy</creatorcontrib><creatorcontrib>Thampuran, Nirmala</creatorcontrib><creatorcontrib>Joseph, Toms C</creatorcontrib><title>Molecular Screening, Isolation, and Characterization of Enterohemorrhagic Escherichia coli O157:H7 from Retail Shrimp</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>Foodborne outbreaks attributed to the contamination of foods with enterohemorrhagic Escherichia coli (EHEC) O157:H7 are a growing global concern. Fish and shrimp samples obtained from different retail fish markets in Cochin, India, were screened by direct PCR assays targeting three important virulence markers of EHEC, the intimin protein (eaeA gene), enterohemolysin (hlyA gene), and Shiga toxin (stx gene). One shrimp (Fenneropenaeus indicus) sample was positive for all these virulence markers, and seven typical E. coli O157:H7 isolates were recovered from the marker-positive shrimp sample. This is the first report of recovery of typical E. coli O157:H7 from fish or shellfish in India. All the typical EHEC isolates had a characteristic reaction in eosin methylene blue agar and belonged to IMViC (indole, methyl red, Voges Proskauer, Simmons citrate reactions) biotype I. These isolates also were negative for sorbitol and methylumbelliferyl-β-glucuronide and exhibited β-hemolytic activity. One isolate showed self-agglutination for E. coli O157 antisera and produced a false-positive reaction with CHROMagar O157. These typical EHEC isolates belonged to a restricted biotype group and had a very low multiple antibiotic resistance index. Isolation of E. coli O157:H7 in fish and shellfish indicates that strict adherence to hygienic handling methods and proper cooking or processing is needed before consumption of these products.</description><subject>Animals</subject><subject>Antibiotic resistance</subject><subject>bacterial contamination</subject><subject>Biological and medical sciences</subject><subject>biotypes</subject><subject>Colony Count, Microbial</subject><subject>Consumer Product Safety</subject><subject>E coli</subject><subject>enrichment culture</subject><subject>enterohemorrhagic Escherichia coli</subject><subject>Enzymes</subject><subject>Epidemics</subject><subject>Escherichia coli O157 - classification</subject><subject>Escherichia coli O157 - genetics</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Escherichia coli O157:H7</subject><subject>Fenneropenaeus indicus</subject><subject>Fish</subject><subject>Fish and seafood industries</subject><subject>Food contamination & poisoning</subject><subject>Food Contamination - analysis</subject><subject>Food Handling - methods</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>Food safety</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>hemolysis</subject><subject>Humans</subject><subject>India</subject><subject>isolation</subject><subject>microbial detection</subject><subject>Molecular biology</subject><subject>molecular systematics</subject><subject>Penaeidae - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>raw fish</subject><subject>retail marketing</subject><subject>ribotypes</subject><subject>seafoods</subject><subject>serotypes</subject><subject>Shellfish</subject><subject>Shellfish - microbiology</subject><subject>shrimp</subject><subject>Toxins</subject><subject>Virulence</subject><subject>Virulence Factors - analysis</subject><subject>Virulence Factors - genetics</subject><subject>virulence genes</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpF0FFv0zAQB3BrGtrK4APsZbOQeFuKz07ieG-oKmzS0CTKJN6sq2M3ntK4s5MH-PS4tIwny-ff3Vl_Qi6BzUsB1Scmal4w3vwspJjDXMkTMgNVloViSp6S2ev7OXmb0jNjjCten5FzzlgFHGBGpm-ht2bqMdKVidYOftjc0PsUehx9GG4oDi1ddBjRjDb633-rNDi6HPI9dHYbYuxw4w1dJtNlYjqP1ITe00eo5O2dpC6GLf1uR_Q9XXXRb3fvyBuHfbLvj-cFefqy_LG4Kx4ev94vPj8URig-FlJJK1u2tsw2lgOWdetqVeKatYpza9GYyoGp21agyXUnSgAHlbCSYYVGXJAPh7m7GF4mm0b9HKY45JWaCxBcihoyggMyMaQUrdO7_EeMvzQwvc9Z73PU-xy1FBq0krnn6jh4Wm9t-9rxL9gMPh4BJoO9izgYn_47LkQtoczu-uAcBo2bmM3TijMQDKRqGtaIPwiJj3U</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Surendraraj, Alagarsamy</creator><creator>Thampuran, Nirmala</creator><creator>Joseph, Toms C</creator><general>International Association for Food Protection</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7WY</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>883</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0F</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope></search><sort><creationdate>2010</creationdate><title>Molecular Screening, Isolation, and Characterization of Enterohemorrhagic Escherichia coli O157:H7 from Retail Shrimp</title><author>Surendraraj, Alagarsamy ; Thampuran, Nirmala ; Joseph, Toms C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-797e7d0be0e8e21a46df694ab0d922eeacc5f1c6dd3ac94af3411f153e70a5ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibiotic resistance</topic><topic>bacterial contamination</topic><topic>Biological and medical sciences</topic><topic>biotypes</topic><topic>Colony Count, Microbial</topic><topic>Consumer Product Safety</topic><topic>E coli</topic><topic>enrichment culture</topic><topic>enterohemorrhagic Escherichia coli</topic><topic>Enzymes</topic><topic>Epidemics</topic><topic>Escherichia coli O157 - classification</topic><topic>Escherichia coli O157 - genetics</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>Escherichia coli O157:H7</topic><topic>Fenneropenaeus indicus</topic><topic>Fish</topic><topic>Fish and seafood industries</topic><topic>Food contamination & poisoning</topic><topic>Food Contamination - analysis</topic><topic>Food Handling - methods</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>Food safety</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>hemolysis</topic><topic>Humans</topic><topic>India</topic><topic>isolation</topic><topic>microbial detection</topic><topic>Molecular biology</topic><topic>molecular systematics</topic><topic>Penaeidae - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>raw fish</topic><topic>retail marketing</topic><topic>ribotypes</topic><topic>seafoods</topic><topic>serotypes</topic><topic>Shellfish</topic><topic>Shellfish - microbiology</topic><topic>shrimp</topic><topic>Toxins</topic><topic>Virulence</topic><topic>Virulence Factors - analysis</topic><topic>Virulence Factors - genetics</topic><topic>virulence genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Surendraraj, Alagarsamy</creatorcontrib><creatorcontrib>Thampuran, Nirmala</creatorcontrib><creatorcontrib>Joseph, Toms C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>ABI/INFORM Collection</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Trade & Industry (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Trade & Industry</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of food protection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Surendraraj, Alagarsamy</au><au>Thampuran, Nirmala</au><au>Joseph, Toms C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Screening, Isolation, and Characterization of Enterohemorrhagic Escherichia coli O157:H7 from Retail Shrimp</atitle><jtitle>Journal of food protection</jtitle><addtitle>J Food Prot</addtitle><date>2010</date><risdate>2010</risdate><volume>73</volume><issue>1</issue><spage>97</spage><epage>103</epage><pages>97-103</pages><issn>0362-028X</issn><eissn>1944-9097</eissn><coden>JFPRDR</coden><abstract>Foodborne outbreaks attributed to the contamination of foods with enterohemorrhagic Escherichia coli (EHEC) O157:H7 are a growing global concern. Fish and shrimp samples obtained from different retail fish markets in Cochin, India, were screened by direct PCR assays targeting three important virulence markers of EHEC, the intimin protein (eaeA gene), enterohemolysin (hlyA gene), and Shiga toxin (stx gene). One shrimp (Fenneropenaeus indicus) sample was positive for all these virulence markers, and seven typical E. coli O157:H7 isolates were recovered from the marker-positive shrimp sample. This is the first report of recovery of typical E. coli O157:H7 from fish or shellfish in India. All the typical EHEC isolates had a characteristic reaction in eosin methylene blue agar and belonged to IMViC (indole, methyl red, Voges Proskauer, Simmons citrate reactions) biotype I. These isolates also were negative for sorbitol and methylumbelliferyl-β-glucuronide and exhibited β-hemolytic activity. One isolate showed self-agglutination for E. coli O157 antisera and produced a false-positive reaction with CHROMagar O157. These typical EHEC isolates belonged to a restricted biotype group and had a very low multiple antibiotic resistance index. Isolation of E. coli O157:H7 in fish and shellfish indicates that strict adherence to hygienic handling methods and proper cooking or processing is needed before consumption of these products.</abstract><cop>Des Moines, IA</cop><pub>International Association for Food Protection</pub><pmid>20051211</pmid><doi>10.4315/0362-028X-73.1.97</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0362-028X |
| ispartof | Journal of food protection, 2010, Vol.73 (1), p.97-103 |
| issn | 0362-028X 1944-9097 |
| language | eng |
| recordid | cdi_proquest_journals_231327361 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antibiotic resistance bacterial contamination Biological and medical sciences biotypes Colony Count, Microbial Consumer Product Safety E coli enrichment culture enterohemorrhagic Escherichia coli Enzymes Epidemics Escherichia coli O157 - classification Escherichia coli O157 - genetics Escherichia coli O157 - isolation & purification Escherichia coli O157:H7 Fenneropenaeus indicus Fish Fish and seafood industries Food contamination & poisoning Food Contamination - analysis Food Handling - methods Food industries Food Microbiology food pathogens Food safety Fundamental and applied biological sciences. Psychology Genes hemolysis Humans India isolation microbial detection Molecular biology molecular systematics Penaeidae - microbiology Polymerase Chain Reaction raw fish retail marketing ribotypes seafoods serotypes Shellfish Shellfish - microbiology shrimp Toxins Virulence Virulence Factors - analysis Virulence Factors - genetics virulence genes |
| title | Molecular Screening, Isolation, and Characterization of Enterohemorrhagic Escherichia coli O157:H7 from Retail Shrimp |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-16T10%3A32%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Screening,%20Isolation,%20and%20Characterization%20of%20Enterohemorrhagic%20Escherichia%20coli%20O157:H7%20from%20Retail%20Shrimp&rft.jtitle=Journal%20of%20food%20protection&rft.au=Surendraraj,%20Alagarsamy&rft.date=2010&rft.volume=73&rft.issue=1&rft.spage=97&rft.epage=103&rft.pages=97-103&rft.issn=0362-028X&rft.eissn=1944-9097&rft.coden=JFPRDR&rft_id=info:doi/10.4315/0362-028X-73.1.97&rft_dat=%3Cproquest_cross%3E1952074881%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=231327361&rft_id=info:pmid/20051211&rfr_iscdi=true |