Fabrication and Characterization of Silk Fibroin Films by a Spin-Coating Method Toward the Application to Field-Effect Transistor-Based Biosensors
Recently, we have succeeded in developing stable and long-term operation of an extended-gate field effect transistor-type glucose sensor using a silk-fibroin film for the immobilization membrane of glucose oxidase. In this paper, we report the fabrication process and characterization of the fibroin...
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| Veröffentlicht in: | Journal of the Society of Materials Science, Japan Japan, 2019/10/15, Vol.68(10), pp.751-756 |
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| container_end_page | 756 |
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| container_title | Journal of the Society of Materials Science, Japan |
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| creator | KOIKE, Kazuto IKE, Kodai ONISHI, Yusuke SASAKI, Taihou HIROFUJI, Yuichi YANO, Mitsuaki |
| description | Recently, we have succeeded in developing stable and long-term operation of an extended-gate field effect transistor-type glucose sensor using a silk-fibroin film for the immobilization membrane of glucose oxidase. In this paper, we report the fabrication process and characterization of the fibroin film. High-purity silk fibroin powder was used as the solute material in water. The advantage of this material is high solubility in water, non-toxicity to human body, and capability of stable immobilization of enzyme molecules. The fibroin film was formed on a glass substrate using a spin coating method followed by an insolubilation treatment in 80 wt% ethanol aqueous solution. It was revealed that the bond strength between the film and the substrate was dramatically improved by applying a silane coupling treatment to the substrate. The obtained film showed β-sheet structure and contained dense nanopores on the surface. |
| doi_str_mv | 10.2472/jsms.68.751 |
| format | Article |
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In this paper, we report the fabrication process and characterization of the fibroin film. High-purity silk fibroin powder was used as the solute material in water. The advantage of this material is high solubility in water, non-toxicity to human body, and capability of stable immobilization of enzyme molecules. The fibroin film was formed on a glass substrate using a spin coating method followed by an insolubilation treatment in 80 wt% ethanol aqueous solution. It was revealed that the bond strength between the film and the substrate was dramatically improved by applying a silane coupling treatment to the substrate. The obtained film showed β-sheet structure and contained dense nanopores on the surface.</description><identifier>ISSN: 0514-5163</identifier><identifier>EISSN: 1880-7488</identifier><identifier>DOI: 10.2472/jsms.68.751</identifier><language>eng ; jpn</language><publisher>Kyoto: The Society of Materials Science, Japan</publisher><subject>Aqueous solutions ; Biosensors ; Bond strength ; Bonding strength ; Coupling (molecular) ; Ethanol ; Field effect transistors ; Glass substrates ; Glucose oxidase ; Immobilization ; Porosity ; Semiconductor devices ; Silk fibroin ; Silk fibroin, Spin-coating, Insolubilation treatment, Enzyme immobilization, FET-based Biosensors ; Spin coating ; Toxicity ; Transistors</subject><ispartof>Journal of the Society of Materials Science, Japan, 2019/10/15, Vol.68(10), pp.751-756</ispartof><rights>2019 by The Society of Materials Science, Japan</rights><rights>Copyright Japan Science and Technology Agency 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2691-7c2a43c24ebc03f9120cc8cad09771bcc3e141eae8488b71b952d0b40d3cab503</citedby><cites>FETCH-LOGICAL-c2691-7c2a43c24ebc03f9120cc8cad09771bcc3e141eae8488b71b952d0b40d3cab503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1881,27922,27923</link.rule.ids></links><search><creatorcontrib>KOIKE, Kazuto</creatorcontrib><creatorcontrib>IKE, Kodai</creatorcontrib><creatorcontrib>ONISHI, Yusuke</creatorcontrib><creatorcontrib>SASAKI, Taihou</creatorcontrib><creatorcontrib>HIROFUJI, Yuichi</creatorcontrib><creatorcontrib>YANO, Mitsuaki</creatorcontrib><title>Fabrication and Characterization of Silk Fibroin Films by a Spin-Coating Method Toward the Application to Field-Effect Transistor-Based Biosensors</title><title>Journal of the Society of Materials Science, Japan</title><addtitle>J. Soc. Mat. Sci., Japan</addtitle><description>Recently, we have succeeded in developing stable and long-term operation of an extended-gate field effect transistor-type glucose sensor using a silk-fibroin film for the immobilization membrane of glucose oxidase. In this paper, we report the fabrication process and characterization of the fibroin film. High-purity silk fibroin powder was used as the solute material in water. The advantage of this material is high solubility in water, non-toxicity to human body, and capability of stable immobilization of enzyme molecules. The fibroin film was formed on a glass substrate using a spin coating method followed by an insolubilation treatment in 80 wt% ethanol aqueous solution. It was revealed that the bond strength between the film and the substrate was dramatically improved by applying a silane coupling treatment to the substrate. The obtained film showed β-sheet structure and contained dense nanopores on the surface.</description><subject>Aqueous solutions</subject><subject>Biosensors</subject><subject>Bond strength</subject><subject>Bonding strength</subject><subject>Coupling (molecular)</subject><subject>Ethanol</subject><subject>Field effect transistors</subject><subject>Glass substrates</subject><subject>Glucose oxidase</subject><subject>Immobilization</subject><subject>Porosity</subject><subject>Semiconductor devices</subject><subject>Silk fibroin</subject><subject>Silk fibroin, Spin-coating, Insolubilation treatment, Enzyme immobilization, FET-based Biosensors</subject><subject>Spin coating</subject><subject>Toxicity</subject><subject>Transistors</subject><issn>0514-5163</issn><issn>1880-7488</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo9kM1O3DAURq2qSIyAFS9gqcsqU_8lcVYVjJgWCcSCYW3Zzg3jacZObaMKHoMnxqOUWV3pu-e7VzoIXVKyZKJlP3Zpn5aNXLY1_YIWVEpStULKr2hBaiqqmjb8FF2k5AwhjDEuRbdA72ttorM6u-Cx9j1ebXXUNkN0b3MYBvzoxj947UwMzpc57hM2r1jjx8n5ahUK55_xPeRt6PEm_NOxx3kL-Gqaxs_TOZQijH11MwxgM95E7ZNLOcTqWifo8bULCXwKMZ2jk0GPCS7-zzP0tL7ZrH5Xdw-_bldXd5VlTUer1jItuGUCjCV86Cgj1kqre9K1LTXWcqCCggZZJJiSdDXriRGk51abmvAz9G2-O8Xw9wVSVrvwEn15qRinlDWEiQP1faZsDClFGNQU3V7HV0WJOnhXB--qkap4L_TPmd6lrJ_hyOqYnR3hyJbu3DhubPGuwPMPkJGPRA</recordid><startdate>20191015</startdate><enddate>20191015</enddate><creator>KOIKE, Kazuto</creator><creator>IKE, Kodai</creator><creator>ONISHI, Yusuke</creator><creator>SASAKI, Taihou</creator><creator>HIROFUJI, Yuichi</creator><creator>YANO, Mitsuaki</creator><general>The Society of Materials Science, Japan</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20191015</creationdate><title>Fabrication and Characterization of Silk Fibroin Films by a Spin-Coating Method Toward the Application to Field-Effect Transistor-Based Biosensors</title><author>KOIKE, Kazuto ; IKE, Kodai ; ONISHI, Yusuke ; SASAKI, Taihou ; HIROFUJI, Yuichi ; YANO, Mitsuaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2691-7c2a43c24ebc03f9120cc8cad09771bcc3e141eae8488b71b952d0b40d3cab503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; jpn</language><creationdate>2019</creationdate><topic>Aqueous solutions</topic><topic>Biosensors</topic><topic>Bond strength</topic><topic>Bonding strength</topic><topic>Coupling (molecular)</topic><topic>Ethanol</topic><topic>Field effect transistors</topic><topic>Glass substrates</topic><topic>Glucose oxidase</topic><topic>Immobilization</topic><topic>Porosity</topic><topic>Semiconductor devices</topic><topic>Silk fibroin</topic><topic>Silk fibroin, Spin-coating, Insolubilation treatment, Enzyme immobilization, FET-based Biosensors</topic><topic>Spin coating</topic><topic>Toxicity</topic><topic>Transistors</topic><toplevel>online_resources</toplevel><creatorcontrib>KOIKE, Kazuto</creatorcontrib><creatorcontrib>IKE, Kodai</creatorcontrib><creatorcontrib>ONISHI, Yusuke</creatorcontrib><creatorcontrib>SASAKI, Taihou</creatorcontrib><creatorcontrib>HIROFUJI, Yuichi</creatorcontrib><creatorcontrib>YANO, Mitsuaki</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Journal of the Society of Materials Science, Japan</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KOIKE, Kazuto</au><au>IKE, Kodai</au><au>ONISHI, Yusuke</au><au>SASAKI, Taihou</au><au>HIROFUJI, Yuichi</au><au>YANO, Mitsuaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fabrication and Characterization of Silk Fibroin Films by a Spin-Coating Method Toward the Application to Field-Effect Transistor-Based Biosensors</atitle><jtitle>Journal of the Society of Materials Science, Japan</jtitle><addtitle>J. Soc. Mat. Sci., Japan</addtitle><date>2019-10-15</date><risdate>2019</risdate><volume>68</volume><issue>10</issue><spage>751</spage><epage>756</epage><pages>751-756</pages><issn>0514-5163</issn><eissn>1880-7488</eissn><abstract>Recently, we have succeeded in developing stable and long-term operation of an extended-gate field effect transistor-type glucose sensor using a silk-fibroin film for the immobilization membrane of glucose oxidase. In this paper, we report the fabrication process and characterization of the fibroin film. High-purity silk fibroin powder was used as the solute material in water. The advantage of this material is high solubility in water, non-toxicity to human body, and capability of stable immobilization of enzyme molecules. The fibroin film was formed on a glass substrate using a spin coating method followed by an insolubilation treatment in 80 wt% ethanol aqueous solution. It was revealed that the bond strength between the film and the substrate was dramatically improved by applying a silane coupling treatment to the substrate. The obtained film showed β-sheet structure and contained dense nanopores on the surface.</abstract><cop>Kyoto</cop><pub>The Society of Materials Science, Japan</pub><doi>10.2472/jsms.68.751</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of the Society of Materials Science, Japan, 2019/10/15, Vol.68(10), pp.751-756 |
| issn | 0514-5163 1880-7488 |
| language | eng ; jpn |
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| source | J-STAGE Free; EZB-FREE-00999 freely available EZB journals |
| subjects | Aqueous solutions Biosensors Bond strength Bonding strength Coupling (molecular) Ethanol Field effect transistors Glass substrates Glucose oxidase Immobilization Porosity Semiconductor devices Silk fibroin Silk fibroin, Spin-coating, Insolubilation treatment, Enzyme immobilization, FET-based Biosensors Spin coating Toxicity Transistors |
| title | Fabrication and Characterization of Silk Fibroin Films by a Spin-Coating Method Toward the Application to Field-Effect Transistor-Based Biosensors |
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