Enzyme Maceration, Fluorescent Staining, and FISH of rDNA of Pineapple (Ananas comosus (L.) Merr.) Chromosomes
Chromosome analysis of pineapple (Ananas comosus (L.) Merr.), one of the most important tropical fruit trees, was conducted. All experiments were carried out using root tips derived from three cross combinations as plant materials. Good preparations, with all 50 chromosomes relatively extended and w...
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| Veröffentlicht in: | Horticulture journal 2019, Vol.88(4), pp.455-461 |
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| creator | Yamamoto, Masashi Takeuchi, Makoto Nashima, Kenji Yamamoto, Toshiya |
| description | Chromosome analysis of pineapple (Ananas comosus (L.) Merr.), one of the most important tropical fruit trees, was conducted. All experiments were carried out using root tips derived from three cross combinations as plant materials. Good preparations, with all 50 chromosomes relatively extended and well-spread without cytoplasm, were observed under enzyme conditions of “2% Cellulase Onozuka RS and 0.5% Pectolyase Y-23” without pretreatment with 2 mM 8-hydroxyquinoline. CMA-positive (+) bands were observed in telomeric positions of two chromosomes. DAPI-negative bands (−) corresponded with CMA+ bands. The numbers and positions of CMA+ bands were stable. Fluorescence in situ hybridization of rDNA was performed. The 18S-5.8S-25S rDNA sites were detected in telomeric positions of two chromosomes. The 5S rDNA sites were also detected in telomeric positions of two chromosomes. The 5S and 18S-5.8S-25S rDNA sites were located on different chromosomes. The 18S-5.8S-25S rDNA sites corresponded with the CMA+/DAPI− bands. The numbers and positions of rDNA sites were stable. |
| doi_str_mv | 10.2503/hortj.UTD-102 |
| format | Article |
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Merr.) Chromosomes</title><source>J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Yamamoto, Masashi ; Takeuchi, Makoto ; Nashima, Kenji ; Yamamoto, Toshiya</creator><creatorcontrib>Yamamoto, Masashi ; Takeuchi, Makoto ; Nashima, Kenji ; Yamamoto, Toshiya</creatorcontrib><description>Chromosome analysis of pineapple (Ananas comosus (L.) Merr.), one of the most important tropical fruit trees, was conducted. All experiments were carried out using root tips derived from three cross combinations as plant materials. Good preparations, with all 50 chromosomes relatively extended and well-spread without cytoplasm, were observed under enzyme conditions of “2% Cellulase Onozuka RS and 0.5% Pectolyase Y-23” without pretreatment with 2 mM 8-hydroxyquinoline. CMA-positive (+) bands were observed in telomeric positions of two chromosomes. DAPI-negative bands (−) corresponded with CMA+ bands. The numbers and positions of CMA+ bands were stable. Fluorescence in situ hybridization of rDNA was performed. The 18S-5.8S-25S rDNA sites were detected in telomeric positions of two chromosomes. The 5S rDNA sites were also detected in telomeric positions of two chromosomes. The 5S and 18S-5.8S-25S rDNA sites were located on different chromosomes. The 18S-5.8S-25S rDNA sites corresponded with the CMA+/DAPI− bands. The numbers and positions of rDNA sites were stable.</description><identifier>ISSN: 2189-0102</identifier><identifier>EISSN: 2189-0110</identifier><identifier>DOI: 10.2503/hortj.UTD-102</identifier><language>eng</language><publisher>Tokyo: The Japanese Society for Horticultural Science</publisher><subject>8-Hydroxyquinoline ; Ananas comosus ; Cellulase ; Chromosomes ; CMA ; Cytoplasm ; DAPI ; EMA ; Enzymes ; Fluorescence ; Fluorescence in situ hybridization ; Fruit trees ; Hydroxyquinoline ; Maceration ; Pectin lyase ; Pineapples ; Pretreatment ; ribosomal RNA gene</subject><ispartof>The Horticulture Journal, 2019, Vol.88(4), pp.455-461</ispartof><rights>2019 The Japanese Society for Horticultural Science (JSHS), All rights reserved.</rights><rights>Copyright Japan Science and Technology Agency 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-4306f939e9312613f0c0721d36de6302bdeb19baed783468afc9e99c67f5d213</citedby><cites>FETCH-LOGICAL-c514t-4306f939e9312613f0c0721d36de6302bdeb19baed783468afc9e99c67f5d213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids></links><search><creatorcontrib>Yamamoto, Masashi</creatorcontrib><creatorcontrib>Takeuchi, Makoto</creatorcontrib><creatorcontrib>Nashima, Kenji</creatorcontrib><creatorcontrib>Yamamoto, Toshiya</creatorcontrib><title>Enzyme Maceration, Fluorescent Staining, and FISH of rDNA of Pineapple (Ananas comosus (L.) Merr.) Chromosomes</title><title>Horticulture journal</title><addtitle>Hort. J.</addtitle><description>Chromosome analysis of pineapple (Ananas comosus (L.) Merr.), one of the most important tropical fruit trees, was conducted. All experiments were carried out using root tips derived from three cross combinations as plant materials. Good preparations, with all 50 chromosomes relatively extended and well-spread without cytoplasm, were observed under enzyme conditions of “2% Cellulase Onozuka RS and 0.5% Pectolyase Y-23” without pretreatment with 2 mM 8-hydroxyquinoline. CMA-positive (+) bands were observed in telomeric positions of two chromosomes. DAPI-negative bands (−) corresponded with CMA+ bands. The numbers and positions of CMA+ bands were stable. Fluorescence in situ hybridization of rDNA was performed. The 18S-5.8S-25S rDNA sites were detected in telomeric positions of two chromosomes. The 5S rDNA sites were also detected in telomeric positions of two chromosomes. The 5S and 18S-5.8S-25S rDNA sites were located on different chromosomes. The 18S-5.8S-25S rDNA sites corresponded with the CMA+/DAPI− bands. The numbers and positions of rDNA sites were stable.</description><subject>8-Hydroxyquinoline</subject><subject>Ananas comosus</subject><subject>Cellulase</subject><subject>Chromosomes</subject><subject>CMA</subject><subject>Cytoplasm</subject><subject>DAPI</subject><subject>EMA</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Fruit trees</subject><subject>Hydroxyquinoline</subject><subject>Maceration</subject><subject>Pectin lyase</subject><subject>Pineapples</subject><subject>Pretreatment</subject><subject>ribosomal RNA gene</subject><issn>2189-0102</issn><issn>2189-0110</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo9kM1rwkAQxUNpoWI99r7QiwVj9yNZk6NErYK2Be15WTcTTUh20914sH99kype5g1vfjMDz_OeCR7TELO3o7FNMf7ezXyC6Z3XoySKfUwIvr_1mD56A-cKjDEJOA8Z7Xl6rn_PFaCNVGBlkxs9QovyZCw4BbpB20bmOteHEZI6RYvVdolMhuzsY9rpV65B1nUJaDjVUkuHlKmMOzk0XI9f0QasbSU52s41Fbgn7yGTpYPBVfvebjHfJUt__fm-SqZrX4UkaPyAYZ7FLIaYEcoJy7DCE0pSxlPgDNN9CnsS7yWkk4gFPJKZatlY8UkWppSwvvdyOVtb83MC14jCnKxuPwrKCKEBD0jcUv6FUtY4ZyETtc0rac-CYNGFKv5DFW2orUNbPrnwhWvkAW60tE2uSrjSUSSCrly3blN1lFaAZn-2CoIN</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Yamamoto, Masashi</creator><creator>Takeuchi, Makoto</creator><creator>Nashima, Kenji</creator><creator>Yamamoto, Toshiya</creator><general>The Japanese Society for Horticultural Science</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20190101</creationdate><title>Enzyme Maceration, Fluorescent Staining, and FISH of rDNA of Pineapple (Ananas comosus (L.) Merr.) Chromosomes</title><author>Yamamoto, Masashi ; Takeuchi, Makoto ; Nashima, Kenji ; Yamamoto, Toshiya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-4306f939e9312613f0c0721d36de6302bdeb19baed783468afc9e99c67f5d213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>8-Hydroxyquinoline</topic><topic>Ananas comosus</topic><topic>Cellulase</topic><topic>Chromosomes</topic><topic>CMA</topic><topic>Cytoplasm</topic><topic>DAPI</topic><topic>EMA</topic><topic>Enzymes</topic><topic>Fluorescence</topic><topic>Fluorescence in situ hybridization</topic><topic>Fruit trees</topic><topic>Hydroxyquinoline</topic><topic>Maceration</topic><topic>Pectin lyase</topic><topic>Pineapples</topic><topic>Pretreatment</topic><topic>ribosomal RNA gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamamoto, Masashi</creatorcontrib><creatorcontrib>Takeuchi, Makoto</creatorcontrib><creatorcontrib>Nashima, Kenji</creatorcontrib><creatorcontrib>Yamamoto, Toshiya</creatorcontrib><collection>CrossRef</collection><jtitle>Horticulture journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamamoto, Masashi</au><au>Takeuchi, Makoto</au><au>Nashima, Kenji</au><au>Yamamoto, Toshiya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme Maceration, Fluorescent Staining, and FISH of rDNA of Pineapple (Ananas comosus (L.) Merr.) Chromosomes</atitle><jtitle>Horticulture journal</jtitle><addtitle>Hort. J.</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>88</volume><issue>4</issue><spage>455</spage><epage>461</epage><pages>455-461</pages><issn>2189-0102</issn><eissn>2189-0110</eissn><abstract>Chromosome analysis of pineapple (Ananas comosus (L.) Merr.), one of the most important tropical fruit trees, was conducted. All experiments were carried out using root tips derived from three cross combinations as plant materials. Good preparations, with all 50 chromosomes relatively extended and well-spread without cytoplasm, were observed under enzyme conditions of “2% Cellulase Onozuka RS and 0.5% Pectolyase Y-23” without pretreatment with 2 mM 8-hydroxyquinoline. CMA-positive (+) bands were observed in telomeric positions of two chromosomes. DAPI-negative bands (−) corresponded with CMA+ bands. The numbers and positions of CMA+ bands were stable. Fluorescence in situ hybridization of rDNA was performed. The 18S-5.8S-25S rDNA sites were detected in telomeric positions of two chromosomes. The 5S rDNA sites were also detected in telomeric positions of two chromosomes. The 5S and 18S-5.8S-25S rDNA sites were located on different chromosomes. The 18S-5.8S-25S rDNA sites corresponded with the CMA+/DAPI− bands. The numbers and positions of rDNA sites were stable.</abstract><cop>Tokyo</cop><pub>The Japanese Society for Horticultural Science</pub><doi>10.2503/hortj.UTD-102</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; EZB-FREE-00999 freely available EZB journals |
| subjects | 8-Hydroxyquinoline Ananas comosus Cellulase Chromosomes CMA Cytoplasm DAPI EMA Enzymes Fluorescence Fluorescence in situ hybridization Fruit trees Hydroxyquinoline Maceration Pectin lyase Pineapples Pretreatment ribosomal RNA gene |
| title | Enzyme Maceration, Fluorescent Staining, and FISH of rDNA of Pineapple (Ananas comosus (L.) Merr.) Chromosomes |
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