Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe
Orientia tsutsugamushi is an obligate intracellular bacterium that causes scrub typhus in humans. Formerly thought to be confined to the “tsutsugamushi triangle” within the Asia–Pacific region, scrub typhus was recently identified in the Western Hemisphere. Moreover, a new species of Orientia bacter...
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| Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2019-11, Vol.19 (11), p.821-827 |
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| creator | Yen, Tsai-Ying Zhang, Zhiwen Chao, Chien-Chung Ching, Wei-Mei Shu, Pei-Yun Tseng, Lien-Feng Carvalho, Arlindo Vicente de Assunção Tsai, Kun-Hsien |
| description | Orientia tsutsugamushi
is an obligate intracellular bacterium that causes scrub typhus in humans. Formerly thought to be confined to the “tsutsugamushi triangle” within the Asia–Pacific region, scrub typhus was recently identified in the Western Hemisphere. Moreover, a new species of
Orientia
bacterial genus was isolated from a patient in Dubai. This study investigated
Orientia
exposure in an African country, the Democratic Republic of Sao Tome and Principe. Two sets of samples were analyzed in the study: 240 dried blood spots (DBSs) collected in 2016 and 863 serum samples from 570 pregnant women in 2003. Antibodies against
O. tsutsugamushi
were examined by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). The positive samples were further confirmed by Western blot. The results of IFA showed that 5.8% (14/240) of DBSs and 20.4% (116/570) of the serum samples contained reactive antibodies, whereas IgG ELISA yielded a positive rate of 15.4% (88/570) for the serum samples. These findings provided serologic evidence of potential
Orientia
exposure even though case of scrub typhus has never been diagnosed in the nation. Further studies are needed to determine the epidemiology and the burden of this neglected tropical disease in Africa. |
| doi_str_mv | 10.1089/vbz.2018.2426 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2311061981</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2311061981</sourcerecordid><originalsourceid>FETCH-LOGICAL-c365t-f1a03cb410e620b65faf298dd1f644ebecb1e86742d07b817edd10f464cbb47d3</originalsourceid><addsrcrecordid>eNqFkEtLxDAURoMovpduJeC6Y26TJu1SdHyAoPjYCSVJbzQy09S0FfXXmzLq1tX9uDn5Eg4hB8BmwMrq-N18zXIG5SwXuVwj21AUKlOqqNanzFnGpVRbZKfvXxnLoYRik2xxEExVkm-Tp3uMYRGevaXzd99ga5G6EOlN9NgOXtP5Rxf6MSL1LR1ekJ7hMtioh3ThDrvRLFIIjt7rQB_CEqluG3obfWt9h3tkw-lFj_s_c5c8ns8fTi-z65uLq9OT68xyWQyZA824NQIYypwZWTjt8qpsGnBSCDRoDWAplcgbpkwJCtMRc0IKa4xQDd8lR6veLoa3Efuhfg1jbNOTdc4BmISqhERlK8rG0PcRXd1Fv9TxswZWTy7r5LKeXNaTy8Qf_rSOZonNH_0rLwF8BUxr3bYLn_4ah39qvwHsj4Du</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2311061981</pqid></control><display><type>article</type><title>Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe</title><source>Alma/SFX Local Collection</source><creator>Yen, Tsai-Ying ; Zhang, Zhiwen ; Chao, Chien-Chung ; Ching, Wei-Mei ; Shu, Pei-Yun ; Tseng, Lien-Feng ; Carvalho, Arlindo Vicente de Assunção ; Tsai, Kun-Hsien</creator><creatorcontrib>Yen, Tsai-Ying ; Zhang, Zhiwen ; Chao, Chien-Chung ; Ching, Wei-Mei ; Shu, Pei-Yun ; Tseng, Lien-Feng ; Carvalho, Arlindo Vicente de Assunção ; Tsai, Kun-Hsien</creatorcontrib><description>Orientia tsutsugamushi
is an obligate intracellular bacterium that causes scrub typhus in humans. Formerly thought to be confined to the “tsutsugamushi triangle” within the Asia–Pacific region, scrub typhus was recently identified in the Western Hemisphere. Moreover, a new species of
Orientia
bacterial genus was isolated from a patient in Dubai. This study investigated
Orientia
exposure in an African country, the Democratic Republic of Sao Tome and Principe. Two sets of samples were analyzed in the study: 240 dried blood spots (DBSs) collected in 2016 and 863 serum samples from 570 pregnant women in 2003. Antibodies against
O. tsutsugamushi
were examined by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). The positive samples were further confirmed by Western blot. The results of IFA showed that 5.8% (14/240) of DBSs and 20.4% (116/570) of the serum samples contained reactive antibodies, whereas IgG ELISA yielded a positive rate of 15.4% (88/570) for the serum samples. These findings provided serologic evidence of potential
Orientia
exposure even though case of scrub typhus has never been diagnosed in the nation. Further studies are needed to determine the epidemiology and the burden of this neglected tropical disease in Africa.</description><identifier>ISSN: 1530-3667</identifier><identifier>EISSN: 1557-7759</identifier><identifier>DOI: 10.1089/vbz.2018.2426</identifier><identifier>PMID: 31407963</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Antibodies ; Enzyme-linked immunosorbent assay ; Epidemiology ; Exposure ; Health risk assessment ; Immunofluorescence ; Immunoglobulin G ; New species ; Orientia tsutsugamushi ; Original Articles ; Pregnancy ; Scrub typhus ; Typhus ; Western Hemisphere</subject><ispartof>Vector borne and zoonotic diseases (Larchmont, N.Y.), 2019-11, Vol.19 (11), p.821-827</ispartof><rights>2019, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. Nov 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-f1a03cb410e620b65faf298dd1f644ebecb1e86742d07b817edd10f464cbb47d3</citedby><cites>FETCH-LOGICAL-c365t-f1a03cb410e620b65faf298dd1f644ebecb1e86742d07b817edd10f464cbb47d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31407963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yen, Tsai-Ying</creatorcontrib><creatorcontrib>Zhang, Zhiwen</creatorcontrib><creatorcontrib>Chao, Chien-Chung</creatorcontrib><creatorcontrib>Ching, Wei-Mei</creatorcontrib><creatorcontrib>Shu, Pei-Yun</creatorcontrib><creatorcontrib>Tseng, Lien-Feng</creatorcontrib><creatorcontrib>Carvalho, Arlindo Vicente de Assunção</creatorcontrib><creatorcontrib>Tsai, Kun-Hsien</creatorcontrib><title>Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe</title><title>Vector borne and zoonotic diseases (Larchmont, N.Y.)</title><addtitle>Vector Borne Zoonotic Dis</addtitle><description>Orientia tsutsugamushi
is an obligate intracellular bacterium that causes scrub typhus in humans. Formerly thought to be confined to the “tsutsugamushi triangle” within the Asia–Pacific region, scrub typhus was recently identified in the Western Hemisphere. Moreover, a new species of
Orientia
bacterial genus was isolated from a patient in Dubai. This study investigated
Orientia
exposure in an African country, the Democratic Republic of Sao Tome and Principe. Two sets of samples were analyzed in the study: 240 dried blood spots (DBSs) collected in 2016 and 863 serum samples from 570 pregnant women in 2003. Antibodies against
O. tsutsugamushi
were examined by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). The positive samples were further confirmed by Western blot. The results of IFA showed that 5.8% (14/240) of DBSs and 20.4% (116/570) of the serum samples contained reactive antibodies, whereas IgG ELISA yielded a positive rate of 15.4% (88/570) for the serum samples. These findings provided serologic evidence of potential
Orientia
exposure even though case of scrub typhus has never been diagnosed in the nation. Further studies are needed to determine the epidemiology and the burden of this neglected tropical disease in Africa.</description><subject>Antibodies</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Epidemiology</subject><subject>Exposure</subject><subject>Health risk assessment</subject><subject>Immunofluorescence</subject><subject>Immunoglobulin G</subject><subject>New species</subject><subject>Orientia tsutsugamushi</subject><subject>Original Articles</subject><subject>Pregnancy</subject><subject>Scrub typhus</subject><subject>Typhus</subject><subject>Western Hemisphere</subject><issn>1530-3667</issn><issn>1557-7759</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkEtLxDAURoMovpduJeC6Y26TJu1SdHyAoPjYCSVJbzQy09S0FfXXmzLq1tX9uDn5Eg4hB8BmwMrq-N18zXIG5SwXuVwj21AUKlOqqNanzFnGpVRbZKfvXxnLoYRik2xxEExVkm-Tp3uMYRGevaXzd99ga5G6EOlN9NgOXtP5Rxf6MSL1LR1ekJ7hMtioh3ThDrvRLFIIjt7rQB_CEqluG3obfWt9h3tkw-lFj_s_c5c8ns8fTi-z65uLq9OT68xyWQyZA824NQIYypwZWTjt8qpsGnBSCDRoDWAplcgbpkwJCtMRc0IKa4xQDd8lR6veLoa3Efuhfg1jbNOTdc4BmISqhERlK8rG0PcRXd1Fv9TxswZWTy7r5LKeXNaTy8Qf_rSOZonNH_0rLwF8BUxr3bYLn_4ah39qvwHsj4Du</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Yen, Tsai-Ying</creator><creator>Zhang, Zhiwen</creator><creator>Chao, Chien-Chung</creator><creator>Ching, Wei-Mei</creator><creator>Shu, Pei-Yun</creator><creator>Tseng, Lien-Feng</creator><creator>Carvalho, Arlindo Vicente de Assunção</creator><creator>Tsai, Kun-Hsien</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope></search><sort><creationdate>20191101</creationdate><title>Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe</title><author>Yen, Tsai-Ying ; Zhang, Zhiwen ; Chao, Chien-Chung ; Ching, Wei-Mei ; Shu, Pei-Yun ; Tseng, Lien-Feng ; Carvalho, Arlindo Vicente de Assunção ; Tsai, Kun-Hsien</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-f1a03cb410e620b65faf298dd1f644ebecb1e86742d07b817edd10f464cbb47d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibodies</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Epidemiology</topic><topic>Exposure</topic><topic>Health risk assessment</topic><topic>Immunofluorescence</topic><topic>Immunoglobulin G</topic><topic>New species</topic><topic>Orientia tsutsugamushi</topic><topic>Original Articles</topic><topic>Pregnancy</topic><topic>Scrub typhus</topic><topic>Typhus</topic><topic>Western Hemisphere</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yen, Tsai-Ying</creatorcontrib><creatorcontrib>Zhang, Zhiwen</creatorcontrib><creatorcontrib>Chao, Chien-Chung</creatorcontrib><creatorcontrib>Ching, Wei-Mei</creatorcontrib><creatorcontrib>Shu, Pei-Yun</creatorcontrib><creatorcontrib>Tseng, Lien-Feng</creatorcontrib><creatorcontrib>Carvalho, Arlindo Vicente de Assunção</creatorcontrib><creatorcontrib>Tsai, Kun-Hsien</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yen, Tsai-Ying</au><au>Zhang, Zhiwen</au><au>Chao, Chien-Chung</au><au>Ching, Wei-Mei</au><au>Shu, Pei-Yun</au><au>Tseng, Lien-Feng</au><au>Carvalho, Arlindo Vicente de Assunção</au><au>Tsai, Kun-Hsien</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe</atitle><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle><addtitle>Vector Borne Zoonotic Dis</addtitle><date>2019-11-01</date><risdate>2019</risdate><volume>19</volume><issue>11</issue><spage>821</spage><epage>827</epage><pages>821-827</pages><issn>1530-3667</issn><eissn>1557-7759</eissn><abstract>Orientia tsutsugamushi
is an obligate intracellular bacterium that causes scrub typhus in humans. Formerly thought to be confined to the “tsutsugamushi triangle” within the Asia–Pacific region, scrub typhus was recently identified in the Western Hemisphere. Moreover, a new species of
Orientia
bacterial genus was isolated from a patient in Dubai. This study investigated
Orientia
exposure in an African country, the Democratic Republic of Sao Tome and Principe. Two sets of samples were analyzed in the study: 240 dried blood spots (DBSs) collected in 2016 and 863 serum samples from 570 pregnant women in 2003. Antibodies against
O. tsutsugamushi
were examined by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). The positive samples were further confirmed by Western blot. The results of IFA showed that 5.8% (14/240) of DBSs and 20.4% (116/570) of the serum samples contained reactive antibodies, whereas IgG ELISA yielded a positive rate of 15.4% (88/570) for the serum samples. These findings provided serologic evidence of potential
Orientia
exposure even though case of scrub typhus has never been diagnosed in the nation. Further studies are needed to determine the epidemiology and the burden of this neglected tropical disease in Africa.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>31407963</pmid><doi>10.1089/vbz.2018.2426</doi><tpages>7</tpages></addata></record> |
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| source | Alma/SFX Local Collection |
| subjects | Antibodies Enzyme-linked immunosorbent assay Epidemiology Exposure Health risk assessment Immunofluorescence Immunoglobulin G New species Orientia tsutsugamushi Original Articles Pregnancy Scrub typhus Typhus Western Hemisphere |
| title | Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe |
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