Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen
Abstract We reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C. Fusobacterium prausnitzii and butyrate-producing bacteria comprise...
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| Veröffentlicht in: | FEMS microbiology letters 2002-02, Vol.208 (1), p.1-7 |
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| container_title | FEMS microbiology letters |
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| creator | Gong, Jianhua Forster, Robert J Yu, Hai Chambers, James R Sabour, Parviz M Wheatcroft, Roger Chen, Shu |
| description | Abstract
We reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C. Fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences. Twenty five percent of the clones had less than 95% homology to database sequences. Many sequences were related to those of uncultured bacteria identified in human feces or the bovine rumen. Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed some differences between bacterial populations present in the mucosa and lumen of ceca. Greater resolution of bacterial population was obtained using a culture-independent approach rather than a culture-based approach. |
| doi_str_mv | 10.1111/j.1574-6968.2002.tb11051.x |
| format | Article |
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We reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C. Fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences. Twenty five percent of the clones had less than 95% homology to database sequences. Many sequences were related to those of uncultured bacteria identified in human feces or the bovine rumen. Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed some differences between bacterial populations present in the mucosa and lumen of ceca. Greater resolution of bacterial population was obtained using a culture-independent approach rather than a culture-based approach.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2002.tb11051.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>16S rRNA ; Bacteria ; Cecal bacterium ; Cecum ; Chicken ; Chickens ; Culture ; Diversity ; Homology ; Microbiology ; Mucosa ; Phylogeny ; Polymorphism ; Populations ; Restriction fragment length polymorphism ; rRNA 16S</subject><ispartof>FEMS microbiology letters, 2002-02, Vol.208 (1), p.1-7</ispartof><rights>2002 Federation of European Microbiological Societies 2002</rights><rights>2002 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2351-1dba1c29d7c76f3a10736ce5f6984e6671893f4c67663ffddbd303460e9781763</citedby><cites>FETCH-LOGICAL-c2351-1dba1c29d7c76f3a10736ce5f6984e6671893f4c67663ffddbd303460e9781763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2002.tb11051.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2002.tb11051.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Gong, Jianhua</creatorcontrib><creatorcontrib>Forster, Robert J</creatorcontrib><creatorcontrib>Yu, Hai</creatorcontrib><creatorcontrib>Chambers, James R</creatorcontrib><creatorcontrib>Sabour, Parviz M</creatorcontrib><creatorcontrib>Wheatcroft, Roger</creatorcontrib><creatorcontrib>Chen, Shu</creatorcontrib><title>Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen</title><title>FEMS microbiology letters</title><description>Abstract
We reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C. Fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences. Twenty five percent of the clones had less than 95% homology to database sequences. Many sequences were related to those of uncultured bacteria identified in human feces or the bovine rumen. Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed some differences between bacterial populations present in the mucosa and lumen of ceca. Greater resolution of bacterial population was obtained using a culture-independent approach rather than a culture-based approach.</description><subject>16S rRNA</subject><subject>Bacteria</subject><subject>Cecal bacterium</subject><subject>Cecum</subject><subject>Chicken</subject><subject>Chickens</subject><subject>Culture</subject><subject>Diversity</subject><subject>Homology</subject><subject>Microbiology</subject><subject>Mucosa</subject><subject>Phylogeny</subject><subject>Polymorphism</subject><subject>Populations</subject><subject>Restriction fragment length polymorphism</subject><subject>rRNA 16S</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqVkMFOhDAQhhujievqOzR6BlsKLRgvZnXVZI0XPTelFCkCxRbc5eC7C-7Gi3qwl0k6_zeT-QA4xcjH4zsvfRyx0KMJjf0AocDvUoxRhP3NHph9t_bBDBEWexgl7BAcOVcihMIA0Rn4uNbvyjrdDVA0GWyLoTIvqlGdluOHqAanHTQ5TIXslNUC6gZ2hYJ1L40TU0cWWr6qBkolxdcMaepWWO1MA9e6K36gU7CCVV-r5hgc5KJy6mRX5-B5efO0uPNWj7f3i6uVJwMSYQ9nqcAySDImGc2JwIgRKlWU0yQOFaUMxwnJQ0kZpSTPsyzNCCIhRSphMWaUzMHZdm5rzVuvXMdL09vxPMcDghFlhART6mKbktY4Z1XOW6trYQeOEZ9085JPTvnklE-6-U4334zw5RZe60oN_yD58mGFRzza4qZv_4C939Z-AvxCl8o</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>Gong, Jianhua</creator><creator>Forster, Robert J</creator><creator>Yu, Hai</creator><creator>Chambers, James R</creator><creator>Sabour, Parviz M</creator><creator>Wheatcroft, Roger</creator><creator>Chen, Shu</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20020201</creationdate><title>Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen</title><author>Gong, Jianhua ; Forster, Robert J ; Yu, Hai ; Chambers, James R ; Sabour, Parviz M ; Wheatcroft, Roger ; Chen, Shu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2351-1dba1c29d7c76f3a10736ce5f6984e6671893f4c67663ffddbd303460e9781763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>16S rRNA</topic><topic>Bacteria</topic><topic>Cecal bacterium</topic><topic>Cecum</topic><topic>Chicken</topic><topic>Chickens</topic><topic>Culture</topic><topic>Diversity</topic><topic>Homology</topic><topic>Microbiology</topic><topic>Mucosa</topic><topic>Phylogeny</topic><topic>Polymorphism</topic><topic>Populations</topic><topic>Restriction fragment length polymorphism</topic><topic>rRNA 16S</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Jianhua</creatorcontrib><creatorcontrib>Forster, Robert J</creatorcontrib><creatorcontrib>Yu, Hai</creatorcontrib><creatorcontrib>Chambers, James R</creatorcontrib><creatorcontrib>Sabour, Parviz M</creatorcontrib><creatorcontrib>Wheatcroft, Roger</creatorcontrib><creatorcontrib>Chen, Shu</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Jianhua</au><au>Forster, Robert J</au><au>Yu, Hai</au><au>Chambers, James R</au><au>Sabour, Parviz M</au><au>Wheatcroft, Roger</au><au>Chen, Shu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen</atitle><jtitle>FEMS microbiology letters</jtitle><date>2002-02-01</date><risdate>2002</risdate><volume>208</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
We reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C. Fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences. Twenty five percent of the clones had less than 95% homology to database sequences. Many sequences were related to those of uncultured bacteria identified in human feces or the bovine rumen. Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed some differences between bacterial populations present in the mucosa and lumen of ceca. Greater resolution of bacterial population was obtained using a culture-independent approach rather than a culture-based approach.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.2002.tb11051.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | FEMS microbiology letters, 2002-02, Vol.208 (1), p.1-7 |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library - AutoHoldings Journals; Alma/SFX Local Collection |
| subjects | 16S rRNA Bacteria Cecal bacterium Cecum Chicken Chickens Culture Diversity Homology Microbiology Mucosa Phylogeny Polymorphism Populations Restriction fragment length polymorphism rRNA 16S |
| title | Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen |
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