Expression of a small RNA, BS203 RNA, from the yocI–yocJ intergenic region of Bacillus subtilis genome
Abstract We isolated and characterized a novel small RNA from Bacillus subtilis. We termed this molecule BS203 RNA from the length of its mature form (203 nt) and located the corresponding gene at the yocI–yocJ intergenic region on the B. subtilis genome. Northern blotting revealed that it is transc...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 2002-01, Vol.207 (1), p.29-33 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 33 |
|---|---|
| container_issue | 1 |
| container_start_page | 29 |
| container_title | FEMS microbiology letters |
| container_volume | 207 |
| creator | Ando, Yoshinari Asari, Sayaka Suzuma, Satoru Yamane, Kunio Nakamura, Kouji |
| description | Abstract
We isolated and characterized a novel small RNA from Bacillus subtilis. We termed this molecule BS203 RNA from the length of its mature form (203 nt) and located the corresponding gene at the yocI–yocJ intergenic region on the B. subtilis genome. Northern blotting revealed that it is transcribed in vegetative growing cells and that the amount of BS203 RNA decreased in the middle of the vegetative phase. A computer-aided prediction of the BS203 RNA secondary structure revealed three characteristic stem–loop structures. Despite active expression during the vegetative phase, growth of the knockout mutant was not affected by depletion of BS203 RNA. A phylogenetic comparison of the sequence of the BS203 RNA with other Bacillus species including B. cereus and B. halodurans C-125, or Clostridium perfringens suggests that the sequence is unique to Bacillus subtilis. |
| doi_str_mv | 10.1111/j.1574-6968.2002.tb11023.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_wiley</sourceid><recordid>TN_cdi_proquest_journals_2310666838</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6968.2002.tb11023.x</oup_id><sourcerecordid>2310666838</sourcerecordid><originalsourceid>FETCH-LOGICAL-o2139-c6733fae0cd5cdab630fbb15125c6f63f2f03c88bfaafe1f2544d35cb3f8e6d53</originalsourceid><addsrcrecordid>eNp1kE1OwzAQhS0EEqVwBwu2JPgncZIFixa1UFRA4mdtOY7dJkriYiei3XEHbshJSJSKFczmjTTvvZE-AM4x8nE3V4WPwyjwWMJinyBE_CbFGBHqbw_A6Pd0CEaIRrGHURIdgxPnCoRQQBAbgfVsu7HKudzU0GgooKtEWcLnx8klnL4QRIdVW1PBZq3gzsjF9-dXJ_cwrxtlV6rOJbRqtW-YCpmXZeuga9MmL3MHO4ep1Ck40qJ06myvY_A2n73e3HnLp9vFzWTpGYJp4kkWUaqFQjILZSZSRpFOUxxiEkqmGdVEIyrjONVCaIU1CYMgo6FMqY4Vy0I6BhdD78aa91a5hhemtXX3khOKEWMspnHnuh5cH3mpdnxj80rYHceI91h5wXt2vGfHe6x8j5Vv-fxhSZIuHw55027-SXt_pOkPyjR-rg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2310666838</pqid></control><display><type>article</type><title>Expression of a small RNA, BS203 RNA, from the yocI–yocJ intergenic region of Bacillus subtilis genome</title><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Ando, Yoshinari ; Asari, Sayaka ; Suzuma, Satoru ; Yamane, Kunio ; Nakamura, Kouji</creator><creatorcontrib>Ando, Yoshinari ; Asari, Sayaka ; Suzuma, Satoru ; Yamane, Kunio ; Nakamura, Kouji</creatorcontrib><description>Abstract
We isolated and characterized a novel small RNA from Bacillus subtilis. We termed this molecule BS203 RNA from the length of its mature form (203 nt) and located the corresponding gene at the yocI–yocJ intergenic region on the B. subtilis genome. Northern blotting revealed that it is transcribed in vegetative growing cells and that the amount of BS203 RNA decreased in the middle of the vegetative phase. A computer-aided prediction of the BS203 RNA secondary structure revealed three characteristic stem–loop structures. Despite active expression during the vegetative phase, growth of the knockout mutant was not affected by depletion of BS203 RNA. A phylogenetic comparison of the sequence of the BS203 RNA with other Bacillus species including B. cereus and B. halodurans C-125, or Clostridium perfringens suggests that the sequence is unique to Bacillus subtilis.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2002.tb11023.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacillus subtilis ; Depletion ; Gene expression ; Genomes ; Intergenic region ; Microbiology ; Non‐coding RNA ; Northern blotting ; Nucleotide sequence ; Pathogens ; Phylogeny ; Protein structure ; Ribonucleic acid ; Riboregulation ; RNA ; Secondary structure ; Small RNA</subject><ispartof>FEMS microbiology letters, 2002-01, Vol.207 (1), p.29-33</ispartof><rights>2002 Federation of European Microbiological Societies 2002</rights><rights>2002 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2002.tb11023.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2002.tb11023.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Ando, Yoshinari</creatorcontrib><creatorcontrib>Asari, Sayaka</creatorcontrib><creatorcontrib>Suzuma, Satoru</creatorcontrib><creatorcontrib>Yamane, Kunio</creatorcontrib><creatorcontrib>Nakamura, Kouji</creatorcontrib><title>Expression of a small RNA, BS203 RNA, from the yocI–yocJ intergenic region of Bacillus subtilis genome</title><title>FEMS microbiology letters</title><description>Abstract
We isolated and characterized a novel small RNA from Bacillus subtilis. We termed this molecule BS203 RNA from the length of its mature form (203 nt) and located the corresponding gene at the yocI–yocJ intergenic region on the B. subtilis genome. Northern blotting revealed that it is transcribed in vegetative growing cells and that the amount of BS203 RNA decreased in the middle of the vegetative phase. A computer-aided prediction of the BS203 RNA secondary structure revealed three characteristic stem–loop structures. Despite active expression during the vegetative phase, growth of the knockout mutant was not affected by depletion of BS203 RNA. A phylogenetic comparison of the sequence of the BS203 RNA with other Bacillus species including B. cereus and B. halodurans C-125, or Clostridium perfringens suggests that the sequence is unique to Bacillus subtilis.</description><subject>Bacillus subtilis</subject><subject>Depletion</subject><subject>Gene expression</subject><subject>Genomes</subject><subject>Intergenic region</subject><subject>Microbiology</subject><subject>Non‐coding RNA</subject><subject>Northern blotting</subject><subject>Nucleotide sequence</subject><subject>Pathogens</subject><subject>Phylogeny</subject><subject>Protein structure</subject><subject>Ribonucleic acid</subject><subject>Riboregulation</subject><subject>RNA</subject><subject>Secondary structure</subject><subject>Small RNA</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE1OwzAQhS0EEqVwBwu2JPgncZIFixa1UFRA4mdtOY7dJkriYiei3XEHbshJSJSKFczmjTTvvZE-AM4x8nE3V4WPwyjwWMJinyBE_CbFGBHqbw_A6Pd0CEaIRrGHURIdgxPnCoRQQBAbgfVsu7HKudzU0GgooKtEWcLnx8klnL4QRIdVW1PBZq3gzsjF9-dXJ_cwrxtlV6rOJbRqtW-YCpmXZeuga9MmL3MHO4ep1Ck40qJ06myvY_A2n73e3HnLp9vFzWTpGYJp4kkWUaqFQjILZSZSRpFOUxxiEkqmGdVEIyrjONVCaIU1CYMgo6FMqY4Vy0I6BhdD78aa91a5hhemtXX3khOKEWMspnHnuh5cH3mpdnxj80rYHceI91h5wXt2vGfHe6x8j5Vv-fxhSZIuHw55027-SXt_pOkPyjR-rg</recordid><startdate>200201</startdate><enddate>200201</enddate><creator>Ando, Yoshinari</creator><creator>Asari, Sayaka</creator><creator>Suzuma, Satoru</creator><creator>Yamane, Kunio</creator><creator>Nakamura, Kouji</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>200201</creationdate><title>Expression of a small RNA, BS203 RNA, from the yocI–yocJ intergenic region of Bacillus subtilis genome</title><author>Ando, Yoshinari ; Asari, Sayaka ; Suzuma, Satoru ; Yamane, Kunio ; Nakamura, Kouji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-o2139-c6733fae0cd5cdab630fbb15125c6f63f2f03c88bfaafe1f2544d35cb3f8e6d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Bacillus subtilis</topic><topic>Depletion</topic><topic>Gene expression</topic><topic>Genomes</topic><topic>Intergenic region</topic><topic>Microbiology</topic><topic>Non‐coding RNA</topic><topic>Northern blotting</topic><topic>Nucleotide sequence</topic><topic>Pathogens</topic><topic>Phylogeny</topic><topic>Protein structure</topic><topic>Ribonucleic acid</topic><topic>Riboregulation</topic><topic>RNA</topic><topic>Secondary structure</topic><topic>Small RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ando, Yoshinari</creatorcontrib><creatorcontrib>Asari, Sayaka</creatorcontrib><creatorcontrib>Suzuma, Satoru</creatorcontrib><creatorcontrib>Yamane, Kunio</creatorcontrib><creatorcontrib>Nakamura, Kouji</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ando, Yoshinari</au><au>Asari, Sayaka</au><au>Suzuma, Satoru</au><au>Yamane, Kunio</au><au>Nakamura, Kouji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a small RNA, BS203 RNA, from the yocI–yocJ intergenic region of Bacillus subtilis genome</atitle><jtitle>FEMS microbiology letters</jtitle><date>2002-01</date><risdate>2002</risdate><volume>207</volume><issue>1</issue><spage>29</spage><epage>33</epage><pages>29-33</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
We isolated and characterized a novel small RNA from Bacillus subtilis. We termed this molecule BS203 RNA from the length of its mature form (203 nt) and located the corresponding gene at the yocI–yocJ intergenic region on the B. subtilis genome. Northern blotting revealed that it is transcribed in vegetative growing cells and that the amount of BS203 RNA decreased in the middle of the vegetative phase. A computer-aided prediction of the BS203 RNA secondary structure revealed three characteristic stem–loop structures. Despite active expression during the vegetative phase, growth of the knockout mutant was not affected by depletion of BS203 RNA. A phylogenetic comparison of the sequence of the BS203 RNA with other Bacillus species including B. cereus and B. halodurans C-125, or Clostridium perfringens suggests that the sequence is unique to Bacillus subtilis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.2002.tb11023.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2002-01, Vol.207 (1), p.29-33 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_journals_2310666838 |
| source | Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Bacillus subtilis Depletion Gene expression Genomes Intergenic region Microbiology Non‐coding RNA Northern blotting Nucleotide sequence Pathogens Phylogeny Protein structure Ribonucleic acid Riboregulation RNA Secondary structure Small RNA |
| title | Expression of a small RNA, BS203 RNA, from the yocI–yocJ intergenic region of Bacillus subtilis genome |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T18%3A24%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_wiley&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20a%20small%20RNA,%20BS203%20RNA,%20from%20the%20yocI%E2%80%93yocJ%20intergenic%20region%20of%20Bacillus%20subtilis%20genome&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Ando,%20Yoshinari&rft.date=2002-01&rft.volume=207&rft.issue=1&rft.spage=29&rft.epage=33&rft.pages=29-33&rft.issn=0378-1097&rft.eissn=1574-6968&rft_id=info:doi/10.1111/j.1574-6968.2002.tb11023.x&rft_dat=%3Cproquest_wiley%3E2310666838%3C/proquest_wiley%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2310666838&rft_id=info:pmid/&rft_oup_id=10.1111/j.1574-6968.2002.tb11023.x&rfr_iscdi=true |