Isolation of Arcobacter species from animal feces
A previously developed Arcobacter isolation protocol for poultry skin and meat was validated for the isolation of Arcobacter from feces of livestock animals. Good repeatability, in-lab reproducibility and sensitivity were achieved and the specificity was improved by additional incorporation of cyclo...
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Veröffentlicht in: | FEMS microbiology letters 2003-12, Vol.229 (2), p.243-248 |
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creator | Ellen Van Driessche Houf, Kurt Jan Van Hoof De Zutter, Lieven Vandamme, Peter |
description | A previously developed Arcobacter isolation protocol for poultry skin and meat was validated for the isolation of Arcobacter from feces of livestock animals. Good repeatability, in-lab reproducibility and sensitivity were achieved and the specificity was improved by additional incorporation of cycloheximide and increase of the novobiocin concentration in the selective supplement. The limit of detection of quantitative and qualitative analysis was 102 and 100 cfu g−1 feces, respectively. From fecal samples collected at slaughterhouse. Arcobacter was isolated from 43.9% of porcine, 39.2% of bovine, 16.1% of ovine and 15.4% of equine samples. All three animal-associated Arcobacter species were isolated and levels up to 103 cfu g−1 feces were determined. |
doi_str_mv | 10.1016/S0378-10970300840-1 |
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Good repeatability, in-lab reproducibility and sensitivity were achieved and the specificity was improved by additional incorporation of cycloheximide and increase of the novobiocin concentration in the selective supplement. The limit of detection of quantitative and qualitative analysis was 102 and 100 cfu g−1 feces, respectively. From fecal samples collected at slaughterhouse. Arcobacter was isolated from 43.9% of porcine, 39.2% of bovine, 16.1% of ovine and 15.4% of equine samples. 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Good repeatability, in-lab reproducibility and sensitivity were achieved and the specificity was improved by additional incorporation of cycloheximide and increase of the novobiocin concentration in the selective supplement. The limit of detection of quantitative and qualitative analysis was 102 and 100 cfu g−1 feces, respectively. From fecal samples collected at slaughterhouse. Arcobacter was isolated from 43.9% of porcine, 39.2% of bovine, 16.1% of ovine and 15.4% of equine samples. All three animal-associated Arcobacter species were isolated and levels up to 103 cfu g−1 feces were determined.</description><subject>Abattoirs</subject><subject>Arcobacter</subject><subject>Cycloheximide</subject><subject>Feces</subject><subject>Livestock</subject><subject>Meat</subject><subject>Microbiology</subject><subject>Novobiocin</subject><subject>Qualitative analysis</subject><subject>Reproducibility</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNyr0KwjAUQOGLKFh_nsAl4By9aWKSjiKKzrqXGBJoaZuatO-vgzg7neF8ABuGO4ZM7u_IlaYMC4UcUQukbAIZOyhBZSH1FLIfmMMipRoRRY4yA3ZLoTFDFToSPDlGG57GDi6S1DtbuUR8DC0xXdWahnhnXVrBzJsmufW3S9hezo_TlfYxvEaXhrIOY-w-q8w5FlwprQX_T70BtdM6Pw</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>Ellen Van Driessche</creator><creator>Houf, Kurt</creator><creator>Jan Van Hoof</creator><creator>De Zutter, Lieven</creator><creator>Vandamme, Peter</creator><general>Oxford University Press</general><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20031201</creationdate><title>Isolation of Arcobacter species from animal feces</title><author>Ellen Van Driessche ; 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source | Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
subjects | Abattoirs Arcobacter Cycloheximide Feces Livestock Meat Microbiology Novobiocin Qualitative analysis Reproducibility |
title | Isolation of Arcobacter species from animal feces |
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