Isolation of Arcobacter species from animal feces

A previously developed Arcobacter isolation protocol for poultry skin and meat was validated for the isolation of Arcobacter from feces of livestock animals. Good repeatability, in-lab reproducibility and sensitivity were achieved and the specificity was improved by additional incorporation of cyclo...

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Veröffentlicht in:FEMS microbiology letters 2003-12, Vol.229 (2), p.243-248
Hauptverfasser: Ellen Van Driessche, Houf, Kurt, Jan Van Hoof, De Zutter, Lieven, Vandamme, Peter
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Houf, Kurt
Jan Van Hoof
De Zutter, Lieven
Vandamme, Peter
description A previously developed Arcobacter isolation protocol for poultry skin and meat was validated for the isolation of Arcobacter from feces of livestock animals. Good repeatability, in-lab reproducibility and sensitivity were achieved and the specificity was improved by additional incorporation of cycloheximide and increase of the novobiocin concentration in the selective supplement. The limit of detection of quantitative and qualitative analysis was 102 and 100 cfu g−1 feces, respectively. From fecal samples collected at slaughterhouse. Arcobacter was isolated from 43.9% of porcine, 39.2% of bovine, 16.1% of ovine and 15.4% of equine samples. All three animal-associated Arcobacter species were isolated and levels up to 103 cfu g−1 feces were determined.
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source Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection
subjects Abattoirs
Arcobacter
Cycloheximide
Feces
Livestock
Meat
Microbiology
Novobiocin
Qualitative analysis
Reproducibility
title Isolation of Arcobacter species from animal feces
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