Impedimetric Biosensor for the Detection of Bacterial Lipopolysaccharide Based on Lectin-functionalized Gold Nanoparticle?Graphene Composites
We developed a simple impedimetric biosensor for the detection of bacterial lipopolysaccharide (LPS) based on lectin-functionalized gold nanoparticle (AuNP)–graphene (G) composites. The effects of AuNPs on G were investigated by comparison with the sensing surface in the absence of AuNPs. The result...
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| Veröffentlicht in: | Sensors and materials 2019-01, Vol.31 (9), p.2917 |
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| creator | Qin, Jianfang Sun, Hong Hao, Haoyong Jia, Lijuan Yao, Chenzhong Wang, Qianqian Yang, Haiying |
| description | We developed a simple impedimetric biosensor for the detection of bacterial lipopolysaccharide (LPS) based on lectin-functionalized gold nanoparticle (AuNP)–graphene (G) composites. The effects of AuNPs on G were investigated by comparison with the sensing surface in the absence of AuNPs. The results showed that the AuNPs formed a 3D micro/ nanocomposite on G, which increased the electroactive area from 7.03 × 10−2 to 1.41 × 10−1 cm2, and also increased the heterogeneous electron transfer rate, which can improve the sensitivity of the biosensor. Concanavalin A (Con A) lectin was used as a molecular recognition element because of its specific interaction toward the carbohydrate groups in LPS. The increase in electron transfer resistance was in a logarithmically direct proportion to the LPS concentration and lowered the detection limit to 600 pg/L. The fabricated biosensor achieved the detection of LPS with good sensitivity, acceptable reproducibility, and stability. This method may hold promise for potential applications in glycomic biosensing. |
| doi_str_mv | 10.18494/SAM.2019.2421 |
| format | Article |
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The effects of AuNPs on G were investigated by comparison with the sensing surface in the absence of AuNPs. The results showed that the AuNPs formed a 3D micro/ nanocomposite on G, which increased the electroactive area from 7.03 × 10−2 to 1.41 × 10−1 cm2, and also increased the heterogeneous electron transfer rate, which can improve the sensitivity of the biosensor. Concanavalin A (Con A) lectin was used as a molecular recognition element because of its specific interaction toward the carbohydrate groups in LPS. The increase in electron transfer resistance was in a logarithmically direct proportion to the LPS concentration and lowered the detection limit to 600 pg/L. The fabricated biosensor achieved the detection of LPS with good sensitivity, acceptable reproducibility, and stability. This method may hold promise for potential applications in glycomic biosensing.</description><identifier>ISSN: 0914-4935</identifier><identifier>DOI: 10.18494/SAM.2019.2421</identifier><language>eng</language><publisher>Tokyo: MYU Scientific Publishing Division</publisher><subject>Biosensors ; Carbohydrates ; Concanavalin A ; Electron transfer ; Gold ; Graphene ; Lectins ; Nanocomposites ; Nanoparticles ; Sensitivity</subject><ispartof>Sensors and materials, 2019-01, Vol.31 (9), p.2917</ispartof><rights>Copyright MYU Scientific Publishing Division 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-8027b3ec69fd594f81a041b1c8a908f11678474ffecccba189c908002af32fdb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids></links><search><creatorcontrib>Qin, Jianfang</creatorcontrib><creatorcontrib>Sun, Hong</creatorcontrib><creatorcontrib>Hao, Haoyong</creatorcontrib><creatorcontrib>Jia, Lijuan</creatorcontrib><creatorcontrib>Yao, Chenzhong</creatorcontrib><creatorcontrib>Wang, Qianqian</creatorcontrib><creatorcontrib>Yang, Haiying</creatorcontrib><title>Impedimetric Biosensor for the Detection of Bacterial Lipopolysaccharide Based on Lectin-functionalized Gold Nanoparticle?Graphene Composites</title><title>Sensors and materials</title><description>We developed a simple impedimetric biosensor for the detection of bacterial lipopolysaccharide (LPS) based on lectin-functionalized gold nanoparticle (AuNP)–graphene (G) composites. The effects of AuNPs on G were investigated by comparison with the sensing surface in the absence of AuNPs. The results showed that the AuNPs formed a 3D micro/ nanocomposite on G, which increased the electroactive area from 7.03 × 10−2 to 1.41 × 10−1 cm2, and also increased the heterogeneous electron transfer rate, which can improve the sensitivity of the biosensor. Concanavalin A (Con A) lectin was used as a molecular recognition element because of its specific interaction toward the carbohydrate groups in LPS. The increase in electron transfer resistance was in a logarithmically direct proportion to the LPS concentration and lowered the detection limit to 600 pg/L. The fabricated biosensor achieved the detection of LPS with good sensitivity, acceptable reproducibility, and stability. This method may hold promise for potential applications in glycomic biosensing.</description><subject>Biosensors</subject><subject>Carbohydrates</subject><subject>Concanavalin A</subject><subject>Electron transfer</subject><subject>Gold</subject><subject>Graphene</subject><subject>Lectins</subject><subject>Nanocomposites</subject><subject>Nanoparticles</subject><subject>Sensitivity</subject><issn>0914-4935</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNotkD1PwzAQhj2ARFW6MltiTvBXvibUFiiVAgzAHDnOWXWVxMF2B_gP_GfcluF00r3PnU4PQjeUpLQUlbh7X76kjNAqZYLRCzQjFRWJqHh2hRbe7wkhtMxIzvIZ-t0OE3RmgOCMwitjPYzeOqxjhR3gBwiggrEjthqvpArgjOxxbSY72f7bS6V20pkOYuihwxGsjwtjog_jaVH25icGG9t3-FWOdpIuGNXD_cbJaQcj4LUdJutNAH-NLrXsPSz--xx9Pj1-rJ-T-m2zXS_rRPGCh6QkrGg5qLzSXVYJXVJJBG2pKmVFSk1pXpSiEFqDUqqVtKxUnBPCpOZMdy2fo9vz3cnZrwP40OztwcVXfcN4JCnPGIlUeqaUs9470M3kzCDdd0NJczLdRNPN0XRzNM3_ALxZdb4</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Qin, Jianfang</creator><creator>Sun, Hong</creator><creator>Hao, Haoyong</creator><creator>Jia, Lijuan</creator><creator>Yao, Chenzhong</creator><creator>Wang, Qianqian</creator><creator>Yang, Haiying</creator><general>MYU Scientific Publishing Division</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20190101</creationdate><title>Impedimetric Biosensor for the Detection of Bacterial Lipopolysaccharide Based on Lectin-functionalized Gold Nanoparticle?Graphene Composites</title><author>Qin, Jianfang ; Sun, Hong ; Hao, Haoyong ; Jia, Lijuan ; Yao, Chenzhong ; Wang, Qianqian ; Yang, Haiying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-8027b3ec69fd594f81a041b1c8a908f11678474ffecccba189c908002af32fdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biosensors</topic><topic>Carbohydrates</topic><topic>Concanavalin A</topic><topic>Electron transfer</topic><topic>Gold</topic><topic>Graphene</topic><topic>Lectins</topic><topic>Nanocomposites</topic><topic>Nanoparticles</topic><topic>Sensitivity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qin, Jianfang</creatorcontrib><creatorcontrib>Sun, Hong</creatorcontrib><creatorcontrib>Hao, Haoyong</creatorcontrib><creatorcontrib>Jia, Lijuan</creatorcontrib><creatorcontrib>Yao, Chenzhong</creatorcontrib><creatorcontrib>Wang, Qianqian</creatorcontrib><creatorcontrib>Yang, Haiying</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qin, Jianfang</au><au>Sun, Hong</au><au>Hao, Haoyong</au><au>Jia, Lijuan</au><au>Yao, Chenzhong</au><au>Wang, Qianqian</au><au>Yang, Haiying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impedimetric Biosensor for the Detection of Bacterial Lipopolysaccharide Based on Lectin-functionalized Gold Nanoparticle?Graphene Composites</atitle><jtitle>Sensors and materials</jtitle><date>2019-01-01</date><risdate>2019</risdate><volume>31</volume><issue>9</issue><spage>2917</spage><pages>2917-</pages><issn>0914-4935</issn><abstract>We developed a simple impedimetric biosensor for the detection of bacterial lipopolysaccharide (LPS) based on lectin-functionalized gold nanoparticle (AuNP)–graphene (G) composites. The effects of AuNPs on G were investigated by comparison with the sensing surface in the absence of AuNPs. The results showed that the AuNPs formed a 3D micro/ nanocomposite on G, which increased the electroactive area from 7.03 × 10−2 to 1.41 × 10−1 cm2, and also increased the heterogeneous electron transfer rate, which can improve the sensitivity of the biosensor. Concanavalin A (Con A) lectin was used as a molecular recognition element because of its specific interaction toward the carbohydrate groups in LPS. The increase in electron transfer resistance was in a logarithmically direct proportion to the LPS concentration and lowered the detection limit to 600 pg/L. The fabricated biosensor achieved the detection of LPS with good sensitivity, acceptable reproducibility, and stability. This method may hold promise for potential applications in glycomic biosensing.</abstract><cop>Tokyo</cop><pub>MYU Scientific Publishing Division</pub><doi>10.18494/SAM.2019.2421</doi><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Biosensors Carbohydrates Concanavalin A Electron transfer Gold Graphene Lectins Nanocomposites Nanoparticles Sensitivity |
| title | Impedimetric Biosensor for the Detection of Bacterial Lipopolysaccharide Based on Lectin-functionalized Gold Nanoparticle?Graphene Composites |
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