A light-up near-infrared probe with aggregation-induced emission characteristics for highly sensitive detection of alkaline phosphatase
Developing activatable near-infrared (NIR) probes to specifically monitor and visualize the activities of cancer-related enzymes is highly significant yet challenging in early cancer diagnosis. Taking advantage of the unique photophysical characteristics of aggregation-induced emission (AIE) fluorop...
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| Veröffentlicht in: | Analyst (London) 2019-11, Vol.144 (21), p.6262-6269 |
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| creator | Zhao, Meng Gao, Yinjia Ye, Shuyue Ding, Jianan Wang, Anna Li, Pengjie Shi, Haibin |
| description | Developing activatable near-infrared (NIR) probes to specifically monitor and visualize the activities of cancer-related enzymes is highly significant yet challenging in early cancer diagnosis. Taking advantage of the unique photophysical characteristics of aggregation-induced emission (AIE) fluorophores, here we design and synthesize a novel activatable probe
QMTP
by conjugating an AIE fluorophore quinolone-malononitrile to a hydrophilic phosphate-modified phenol group. The probe was initially non-fluorescent in aqueous solution due to its good water solubility, but was readily activated to generate a strong NIR fluorescence upon treatment with alkaline phosphatase (ALP), which enables specific detection of ALP activity. Furthermore, we have employed
QMTP
to monitor and spatially map the activity of endogenous ALP both in cancer cells and in drug-treated zebrafish larvae. The experimental results reveal that the
QMTP
probe has great specificity and sensitivity for ALP detection. We thus believe that our work offers a promising tool for accurate detection of ALP-associated diseases in preclinical applications.
A new light-up near-infrared probe with aggregation-induced emission (AIE) characteristics was developed for highly specific and sensitive detection of alkaline phosphatase both
in vitro
and
in vivo
. |
| doi_str_mv | 10.1039/c9an01505a |
| format | Article |
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QMTP
by conjugating an AIE fluorophore quinolone-malononitrile to a hydrophilic phosphate-modified phenol group. The probe was initially non-fluorescent in aqueous solution due to its good water solubility, but was readily activated to generate a strong NIR fluorescence upon treatment with alkaline phosphatase (ALP), which enables specific detection of ALP activity. Furthermore, we have employed
QMTP
to monitor and spatially map the activity of endogenous ALP both in cancer cells and in drug-treated zebrafish larvae. The experimental results reveal that the
QMTP
probe has great specificity and sensitivity for ALP detection. We thus believe that our work offers a promising tool for accurate detection of ALP-associated diseases in preclinical applications.
A new light-up near-infrared probe with aggregation-induced emission (AIE) characteristics was developed for highly specific and sensitive detection of alkaline phosphatase both
in vitro
and
in vivo
.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c9an01505a</identifier><identifier>PMID: 31566642</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>3T3 Cells ; Agglomeration ; Alkaline phosphatase ; Alkaline Phosphatase - chemistry ; Alkaline Phosphatase - metabolism ; Animals ; Aqueous solutions ; Cancer ; Chemical compounds ; Emission ; Flow Cytometry ; Fluorescence ; Fluorescent Dyes - chemistry ; HeLa Cells ; Humans ; Hydrophobic and Hydrophilic Interactions ; Infrared Rays ; Larva - enzymology ; Larvae ; Limit of Detection ; Malononitrile ; Mice ; Near infrared radiation ; Nitriles - chemistry ; Optical Imaging ; Phosphatase ; Quinolones - chemistry ; Zebrafish</subject><ispartof>Analyst (London), 2019-11, Vol.144 (21), p.6262-6269</ispartof><rights>Copyright Royal Society of Chemistry 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-924fcc84fe4ce1b1964d7ef23ab8e15b025743133a5611c5f240a89773dcdc843</citedby><cites>FETCH-LOGICAL-c363t-924fcc84fe4ce1b1964d7ef23ab8e15b025743133a5611c5f240a89773dcdc843</cites><orcidid>0000-0003-2234-9126</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2818,2819,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31566642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Meng</creatorcontrib><creatorcontrib>Gao, Yinjia</creatorcontrib><creatorcontrib>Ye, Shuyue</creatorcontrib><creatorcontrib>Ding, Jianan</creatorcontrib><creatorcontrib>Wang, Anna</creatorcontrib><creatorcontrib>Li, Pengjie</creatorcontrib><creatorcontrib>Shi, Haibin</creatorcontrib><title>A light-up near-infrared probe with aggregation-induced emission characteristics for highly sensitive detection of alkaline phosphatase</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Developing activatable near-infrared (NIR) probes to specifically monitor and visualize the activities of cancer-related enzymes is highly significant yet challenging in early cancer diagnosis. Taking advantage of the unique photophysical characteristics of aggregation-induced emission (AIE) fluorophores, here we design and synthesize a novel activatable probe
QMTP
by conjugating an AIE fluorophore quinolone-malononitrile to a hydrophilic phosphate-modified phenol group. The probe was initially non-fluorescent in aqueous solution due to its good water solubility, but was readily activated to generate a strong NIR fluorescence upon treatment with alkaline phosphatase (ALP), which enables specific detection of ALP activity. Furthermore, we have employed
QMTP
to monitor and spatially map the activity of endogenous ALP both in cancer cells and in drug-treated zebrafish larvae. The experimental results reveal that the
QMTP
probe has great specificity and sensitivity for ALP detection. We thus believe that our work offers a promising tool for accurate detection of ALP-associated diseases in preclinical applications.
A new light-up near-infrared probe with aggregation-induced emission (AIE) characteristics was developed for highly specific and sensitive detection of alkaline phosphatase both
in vitro
and
in vivo
.</description><subject>3T3 Cells</subject><subject>Agglomeration</subject><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - chemistry</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Aqueous solutions</subject><subject>Cancer</subject><subject>Chemical compounds</subject><subject>Emission</subject><subject>Flow Cytometry</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Infrared Rays</subject><subject>Larva - enzymology</subject><subject>Larvae</subject><subject>Limit of Detection</subject><subject>Malononitrile</subject><subject>Mice</subject><subject>Near infrared radiation</subject><subject>Nitriles - chemistry</subject><subject>Optical Imaging</subject><subject>Phosphatase</subject><subject>Quinolones - chemistry</subject><subject>Zebrafish</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhq0K1G4Ll96pjHpBSAF_JzmuVnxJFb2UczRxxhuXbBJsB9RfwN_Gy7ZF6oGTNX6feTUzLyHnnL3jTNbvbQ0j45ppOCIrLo0qtBbVM7JijMlCGK1OyGmMt7nkTLNjciK5NsYosSK_13Tw2z4Vy0xHhFD40QUI2NE5TC3SXz71FLbbgFtIfhqz3i02y7jzMeYPansIYBMGH5O3kbop0D5bDnc04hh98j-RdpjQ7vvp5CgM32HwI9K5n-LcQ4KIL8hzB0PEl_fvGfn28cPN5nNxdf3py2Z9VVhpZCpqoZy1lXKoLPKW10Z1JTohoa2Q65YJXSrJpQRtOLfaCcWgqstSdrbLffKMvDn45vV-LBhTk_ewOAww4rTERoi6VspUfI9ePkFvpyWMebpGSFbmG5ZMZ-rtgbJhijGga-bgdxDuGs6afTzNpl5__RvPOsMX95ZLu8PuEX3IIwOvD0CI9lH9l28zdy4zr_7HyD-3jqGr</recordid><startdate>20191107</startdate><enddate>20191107</enddate><creator>Zhao, Meng</creator><creator>Gao, Yinjia</creator><creator>Ye, Shuyue</creator><creator>Ding, Jianan</creator><creator>Wang, Anna</creator><creator>Li, Pengjie</creator><creator>Shi, Haibin</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2234-9126</orcidid></search><sort><creationdate>20191107</creationdate><title>A light-up near-infrared probe with aggregation-induced emission characteristics for highly sensitive detection of alkaline phosphatase</title><author>Zhao, Meng ; Gao, Yinjia ; Ye, Shuyue ; Ding, Jianan ; Wang, Anna ; Li, Pengjie ; Shi, Haibin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-924fcc84fe4ce1b1964d7ef23ab8e15b025743133a5611c5f240a89773dcdc843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>3T3 Cells</topic><topic>Agglomeration</topic><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - chemistry</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Aqueous solutions</topic><topic>Cancer</topic><topic>Chemical compounds</topic><topic>Emission</topic><topic>Flow Cytometry</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Infrared Rays</topic><topic>Larva - enzymology</topic><topic>Larvae</topic><topic>Limit of Detection</topic><topic>Malononitrile</topic><topic>Mice</topic><topic>Near infrared radiation</topic><topic>Nitriles - chemistry</topic><topic>Optical Imaging</topic><topic>Phosphatase</topic><topic>Quinolones - chemistry</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Meng</creatorcontrib><creatorcontrib>Gao, Yinjia</creatorcontrib><creatorcontrib>Ye, Shuyue</creatorcontrib><creatorcontrib>Ding, Jianan</creatorcontrib><creatorcontrib>Wang, Anna</creatorcontrib><creatorcontrib>Li, Pengjie</creatorcontrib><creatorcontrib>Shi, Haibin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Meng</au><au>Gao, Yinjia</au><au>Ye, Shuyue</au><au>Ding, Jianan</au><au>Wang, Anna</au><au>Li, Pengjie</au><au>Shi, Haibin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A light-up near-infrared probe with aggregation-induced emission characteristics for highly sensitive detection of alkaline phosphatase</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2019-11-07</date><risdate>2019</risdate><volume>144</volume><issue>21</issue><spage>6262</spage><epage>6269</epage><pages>6262-6269</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Developing activatable near-infrared (NIR) probes to specifically monitor and visualize the activities of cancer-related enzymes is highly significant yet challenging in early cancer diagnosis. Taking advantage of the unique photophysical characteristics of aggregation-induced emission (AIE) fluorophores, here we design and synthesize a novel activatable probe
QMTP
by conjugating an AIE fluorophore quinolone-malononitrile to a hydrophilic phosphate-modified phenol group. The probe was initially non-fluorescent in aqueous solution due to its good water solubility, but was readily activated to generate a strong NIR fluorescence upon treatment with alkaline phosphatase (ALP), which enables specific detection of ALP activity. Furthermore, we have employed
QMTP
to monitor and spatially map the activity of endogenous ALP both in cancer cells and in drug-treated zebrafish larvae. The experimental results reveal that the
QMTP
probe has great specificity and sensitivity for ALP detection. We thus believe that our work offers a promising tool for accurate detection of ALP-associated diseases in preclinical applications.
A new light-up near-infrared probe with aggregation-induced emission (AIE) characteristics was developed for highly specific and sensitive detection of alkaline phosphatase both
in vitro
and
in vivo
.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>31566642</pmid><doi>10.1039/c9an01505a</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2234-9126</orcidid></addata></record> |
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| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | 3T3 Cells Agglomeration Alkaline phosphatase Alkaline Phosphatase - chemistry Alkaline Phosphatase - metabolism Animals Aqueous solutions Cancer Chemical compounds Emission Flow Cytometry Fluorescence Fluorescent Dyes - chemistry HeLa Cells Humans Hydrophobic and Hydrophilic Interactions Infrared Rays Larva - enzymology Larvae Limit of Detection Malononitrile Mice Near infrared radiation Nitriles - chemistry Optical Imaging Phosphatase Quinolones - chemistry Zebrafish |
| title | A light-up near-infrared probe with aggregation-induced emission characteristics for highly sensitive detection of alkaline phosphatase |
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