Overproduction of human α-2b interferon in a soluble form in Escherichia colicells using the bacteriophage T7 RNA polymerase-base expression system

Overproduction of human α-2b interferon in a soluble form in Escherichia colicells using the bacteriophage T7 RNA polymerase-base expression system

An effective technology of soluble human α-2b interferon (IFN) production using the bacteriophage T7 polymerase-base expression system have been developed. For this purpose an artificial gene for human α-2b interferon (IFN) has been chemically synthesized and cloned into the expression vector pET-24...

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Veröffentlicht in:Biopolimery i kletka 2003-01, Vol.19 (4), p.367
Hauptverfasser: Yu, Slavchenko I, Boreyko, E V, Vorobey, N V, Chernykh, S I, Kordyum, V A
Format: Artikel
Sprache:eng
Schlagworte:
DNA-directed RNA polymerase
Interferon
RNA phages
RNA polymerase
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Zusammenfassung:An effective technology of soluble human α-2b interferon (IFN) production using the bacteriophage T7 polymerase-base expression system have been developed. For this purpose an artificial gene for human α-2b interferon (IFN) has been chemically synthesized and cloned into the expression vector pET-24a containing T7 promoter and terminator. A recombinant plasmid named pET-alFN was transformed into
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.000669