The efficient method for DNA encapsulation into reconstituted sendai virus envelopes
The freezing-thawing method is used for encapsulation up to 17000 base pairs DNA into reconstituted Sendai virus envelopes (RSVE). It permits encapsulating up to 30 % of DNA added to the solution. The interaction RSVE loaded with plasmid DNA and Krebs-2 ascites tumours cells results in up to 30 % pe...
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Veröffentlicht in: | Biopolimery i kletka 1988-09, Vol.4 (5), p.250-254 |
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container_title | Biopolimery i kletka |
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creator | Vlassov, V. V. Krendelev, Yu. D. Ovander, M. N. Ryte, A. S. Repin, V. E. Svinarchuk, F. P. |
description | The freezing-thawing method is used for encapsulation up to 17000 base pairs DNA into reconstituted Sendai virus envelopes (RSVE). It permits encapsulating up to 30 % of DNA added to the solution. The interaction RSVE loaded with plasmid DNA and Krebs-2 ascites tumours cells results in up to 30 % penetration of the encapsulated material into the cells. |
doi_str_mv | 10.7124/bc.000233 |
format | Article |
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source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
subjects | Ascites Deoxyribonucleic acid DNA Encapsulation Envelopes Freezing Thawing |
title | The efficient method for DNA encapsulation into reconstituted sendai virus envelopes |
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