Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)
SUMMARY The unicellular green alga Dunaliella salina is an attractive organism for studying photoacclimation responses. Changes in irradiance level during cell growth affect the organization and structure of the photosystem and the composition of pigments. In this study, D. salina was exposed to dif...
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| Veröffentlicht in: | Phycological research 2019-10, Vol.67 (4), p.291-302 |
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| creator | Li, Yuanxiang Gu, Wenhui Huang, Aiyou Xie, Xiujun Wu, Songcui Wang, Guangce |
| description | SUMMARY
The unicellular green alga Dunaliella salina is an attractive organism for studying photoacclimation responses. Changes in irradiance level during cell growth affect the organization and structure of the photosystem and the composition of pigments. In this study, D. salina was exposed to different irradiance levels, and cell growth, photosynthetic activity, pigments, and neutral lipids were analyzed. Photosystem II activity and chlorophyll content were reduced, carotenoids content and neutral lipids increased as light intensity increased, suggesting that photosynthetic apparatus was reduced and photoprotection mechanisms were activated. The RNA of D. salina was sequenced to investigate the transcriptomic response of the organism after transitioning from normal light conditions to higher light intensity. Genes encoding for enzymes involved in photosynthesis were downregulated, whereas genes involved in the metabolism of carotenoid and triacylglycerol were upregulated. Genes encoding for photoprotective enzymes related to reactive oxygen species scavenging and to the xanthophyll cycle were also upregulated at higher irradiance levels. The present transcriptomic study would assist in the comprehensive understanding of photoacclimation mechanisms of D. salina. |
| doi_str_mv | 10.1111/pre.12379 |
| format | Article |
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The unicellular green alga Dunaliella salina is an attractive organism for studying photoacclimation responses. Changes in irradiance level during cell growth affect the organization and structure of the photosystem and the composition of pigments. In this study, D. salina was exposed to different irradiance levels, and cell growth, photosynthetic activity, pigments, and neutral lipids were analyzed. Photosystem II activity and chlorophyll content were reduced, carotenoids content and neutral lipids increased as light intensity increased, suggesting that photosynthetic apparatus was reduced and photoprotection mechanisms were activated. The RNA of D. salina was sequenced to investigate the transcriptomic response of the organism after transitioning from normal light conditions to higher light intensity. Genes encoding for enzymes involved in photosynthesis were downregulated, whereas genes involved in the metabolism of carotenoid and triacylglycerol were upregulated. Genes encoding for photoprotective enzymes related to reactive oxygen species scavenging and to the xanthophyll cycle were also upregulated at higher irradiance levels. The present transcriptomic study would assist in the comprehensive understanding of photoacclimation mechanisms of D. salina.</description><identifier>ISSN: 1322-0829</identifier><identifier>EISSN: 1440-1835</identifier><identifier>DOI: 10.1111/pre.12379</identifier><language>eng</language><publisher>Kyoto, Japan: John Wiley & Sons Australia, Ltd</publisher><subject>Acclimation ; Aquatic plants ; carotenoid ; Carotenoids ; Cell growth ; Chlorophyll ; Chlorophylls ; Dunaliella salina ; Enzymes ; Gene expression ; gene expression regulation ; Genes ; Irradiance ; Levels ; Light effects ; Light intensity ; light treatment ; Lipids ; Luminous intensity ; lutein ; Metabolism ; microalgae ; Nucleic acids ; Organizations ; Photosynthesis ; photosynthetic activity ; Photosynthetic apparatus ; Photosystem II ; Pigments ; radiation resistance ; Reactive oxygen species ; Ribonucleic acid ; RNA ; Scavenging ; transcriptomics ; triacylglycerols ; Triglycerides ; Xanthophylls</subject><ispartof>Phycological research, 2019-10, Vol.67 (4), p.291-302</ispartof><rights>2019 Japanese Society of Phycology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3309-598b9187e58571686d73751cb6f50ac9ac60122fdab680b310014dee5d4db6563</citedby><cites>FETCH-LOGICAL-c3309-598b9187e58571686d73751cb6f50ac9ac60122fdab680b310014dee5d4db6563</cites><orcidid>0000-0001-9307-3994</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpre.12379$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpre.12379$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids></links><search><creatorcontrib>Li, Yuanxiang</creatorcontrib><creatorcontrib>Gu, Wenhui</creatorcontrib><creatorcontrib>Huang, Aiyou</creatorcontrib><creatorcontrib>Xie, Xiujun</creatorcontrib><creatorcontrib>Wu, Songcui</creatorcontrib><creatorcontrib>Wang, Guangce</creatorcontrib><title>Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)</title><title>Phycological research</title><description>SUMMARY
The unicellular green alga Dunaliella salina is an attractive organism for studying photoacclimation responses. Changes in irradiance level during cell growth affect the organization and structure of the photosystem and the composition of pigments. In this study, D. salina was exposed to different irradiance levels, and cell growth, photosynthetic activity, pigments, and neutral lipids were analyzed. Photosystem II activity and chlorophyll content were reduced, carotenoids content and neutral lipids increased as light intensity increased, suggesting that photosynthetic apparatus was reduced and photoprotection mechanisms were activated. The RNA of D. salina was sequenced to investigate the transcriptomic response of the organism after transitioning from normal light conditions to higher light intensity. Genes encoding for enzymes involved in photosynthesis were downregulated, whereas genes involved in the metabolism of carotenoid and triacylglycerol were upregulated. Genes encoding for photoprotective enzymes related to reactive oxygen species scavenging and to the xanthophyll cycle were also upregulated at higher irradiance levels. The present transcriptomic study would assist in the comprehensive understanding of photoacclimation mechanisms of D. salina.</description><subject>Acclimation</subject><subject>Aquatic plants</subject><subject>carotenoid</subject><subject>Carotenoids</subject><subject>Cell growth</subject><subject>Chlorophyll</subject><subject>Chlorophylls</subject><subject>Dunaliella salina</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Genes</subject><subject>Irradiance</subject><subject>Levels</subject><subject>Light effects</subject><subject>Light intensity</subject><subject>light treatment</subject><subject>Lipids</subject><subject>Luminous intensity</subject><subject>lutein</subject><subject>Metabolism</subject><subject>microalgae</subject><subject>Nucleic acids</subject><subject>Organizations</subject><subject>Photosynthesis</subject><subject>photosynthetic activity</subject><subject>Photosynthetic apparatus</subject><subject>Photosystem II</subject><subject>Pigments</subject><subject>radiation resistance</subject><subject>Reactive oxygen species</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Scavenging</subject><subject>transcriptomics</subject><subject>triacylglycerols</subject><subject>Triglycerides</subject><subject>Xanthophylls</subject><issn>1322-0829</issn><issn>1440-1835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kU1LxDAQhosouH4c_AcBL-uhbpI2bXOUdf2ABUX0XNJ0uo1kk5q06v4O_7Cp9SQYAjMJz7xvMhNFZwRfkrAWnYNLQpOc70UzkqY4JkXC9kOeUBrjgvLD6Mj7V4wxZQWfRV_PThgvnep6uwUkjNA7rzxy8A5Cj3EzaNEra5Bt0AYMIPgMJt6PV_XglNmgrrW9FVJqtZ3Q3qJWbVqknBO1EkYC0kEw6CmDrodgokBrgXxIjEDzZauts127kyDg4iQ6aII3nP7G4-jlZvW8vIvXD7f3y6t1LJME85jxouKkyIEVLCdZkdV5kjMiq6xhWEguZIYJpU0tqqzAVUIwJmkNwOq0rjKWJcfRfNLtnH0bwPflVnk5PsyAHXwZ2pgSPu6Anv9BX-3gwj9GCmcMU8ppoC4mSjrrvYOm7FxoiduVBJfjeMIZyp_xBHYxsR9Kw-5_sHx8Wk0V386ck9s</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Li, Yuanxiang</creator><creator>Gu, Wenhui</creator><creator>Huang, Aiyou</creator><creator>Xie, Xiujun</creator><creator>Wu, Songcui</creator><creator>Wang, Guangce</creator><general>John Wiley & Sons Australia, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-9307-3994</orcidid></search><sort><creationdate>201910</creationdate><title>Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)</title><author>Li, Yuanxiang ; Gu, Wenhui ; Huang, Aiyou ; Xie, Xiujun ; Wu, Songcui ; Wang, Guangce</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3309-598b9187e58571686d73751cb6f50ac9ac60122fdab680b310014dee5d4db6563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acclimation</topic><topic>Aquatic plants</topic><topic>carotenoid</topic><topic>Carotenoids</topic><topic>Cell growth</topic><topic>Chlorophyll</topic><topic>Chlorophylls</topic><topic>Dunaliella salina</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>Genes</topic><topic>Irradiance</topic><topic>Levels</topic><topic>Light effects</topic><topic>Light intensity</topic><topic>light treatment</topic><topic>Lipids</topic><topic>Luminous intensity</topic><topic>lutein</topic><topic>Metabolism</topic><topic>microalgae</topic><topic>Nucleic acids</topic><topic>Organizations</topic><topic>Photosynthesis</topic><topic>photosynthetic activity</topic><topic>Photosynthetic apparatus</topic><topic>Photosystem II</topic><topic>Pigments</topic><topic>radiation resistance</topic><topic>Reactive oxygen species</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Scavenging</topic><topic>transcriptomics</topic><topic>triacylglycerols</topic><topic>Triglycerides</topic><topic>Xanthophylls</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yuanxiang</creatorcontrib><creatorcontrib>Gu, Wenhui</creatorcontrib><creatorcontrib>Huang, Aiyou</creatorcontrib><creatorcontrib>Xie, Xiujun</creatorcontrib><creatorcontrib>Wu, Songcui</creatorcontrib><creatorcontrib>Wang, Guangce</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Phycological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yuanxiang</au><au>Gu, Wenhui</au><au>Huang, Aiyou</au><au>Xie, Xiujun</au><au>Wu, Songcui</au><au>Wang, Guangce</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)</atitle><jtitle>Phycological research</jtitle><date>2019-10</date><risdate>2019</risdate><volume>67</volume><issue>4</issue><spage>291</spage><epage>302</epage><pages>291-302</pages><issn>1322-0829</issn><eissn>1440-1835</eissn><abstract>SUMMARY
The unicellular green alga Dunaliella salina is an attractive organism for studying photoacclimation responses. Changes in irradiance level during cell growth affect the organization and structure of the photosystem and the composition of pigments. In this study, D. salina was exposed to different irradiance levels, and cell growth, photosynthetic activity, pigments, and neutral lipids were analyzed. Photosystem II activity and chlorophyll content were reduced, carotenoids content and neutral lipids increased as light intensity increased, suggesting that photosynthetic apparatus was reduced and photoprotection mechanisms were activated. The RNA of D. salina was sequenced to investigate the transcriptomic response of the organism after transitioning from normal light conditions to higher light intensity. Genes encoding for enzymes involved in photosynthesis were downregulated, whereas genes involved in the metabolism of carotenoid and triacylglycerol were upregulated. Genes encoding for photoprotective enzymes related to reactive oxygen species scavenging and to the xanthophyll cycle were also upregulated at higher irradiance levels. The present transcriptomic study would assist in the comprehensive understanding of photoacclimation mechanisms of D. salina.</abstract><cop>Kyoto, Japan</cop><pub>John Wiley & Sons Australia, Ltd</pub><doi>10.1111/pre.12379</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9307-3994</orcidid></addata></record> |
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| subjects | Acclimation Aquatic plants carotenoid Carotenoids Cell growth Chlorophyll Chlorophylls Dunaliella salina Enzymes Gene expression gene expression regulation Genes Irradiance Levels Light effects Light intensity light treatment Lipids Luminous intensity lutein Metabolism microalgae Nucleic acids Organizations Photosynthesis photosynthetic activity Photosynthetic apparatus Photosystem II Pigments radiation resistance Reactive oxygen species Ribonucleic acid RNA Scavenging transcriptomics triacylglycerols Triglycerides Xanthophylls |
| title | Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae) |
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