Application of silicalite for improvement of enzyme adsorption on the stainless steel electrodes
Aim. Improvement of analytical characteristics of an enzyme biosensor based on new inexpensive perspective stainless steel electrodes using silicalite nanoparticles. Methods. Conductometric enzyme biosensor was used. Results. Three methods of glucose oxidase (GOx) immobilization were studied and com...
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| Veröffentlicht in: | Biopolimery i kletka 2014, Vol.30 (6), p.462-468 |
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| creator | Pyeshkova, V. N. Dudchenko, O. Y. Soldatkin, O. O. Kucherenko, I. S. Ozansoy Kasap, B. Akata Kurc, B. Dzyadevych, S. V. |
| description | Aim. Improvement of analytical characteristics of an enzyme biosensor based on new inexpensive perspective stainless steel electrodes using silicalite nanoparticles. Methods. Conductometric enzyme biosensor was used. Results. Three methods of glucose oxidase (GOx) immobilization were studied and compared: GOx adsorption on silicalite modified electrodes (GOx-SME); cross-linking by glutaraldehyde without silicalite (GOx-GA); GOx adsorption on SME along with cross-linking by glutaraldehyde (GOx-SME-GA). The GOx-SME-GA biosensors based on stainless steel electrodes were characterized by 12–25-fold higher sensitivity comparing with other biosensors. The developed GOx-SME-GA biosensors were characterized by good reproducibility of glucose biosensors construction (relative standard deviation (RSD) – 18 %), improved signal reproducibility (RSD of glucose determination was 7 %) and good storage stability (29 % loss of activity after 18 days). Conclusions. The method of enzyme immobilization using silicalite together with GA cross-linking sufficiently enhances the enzyme adsorption on the stainless steel electrodes and improves the analytical parameters of biosensors. This method is found to be promising for further creation of other enzyme biosensors. |
| doi_str_mv | 10.7124/bc.0008C1 |
| format | Article |
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N. ; Dudchenko, O. Y. ; Soldatkin, O. O. ; Kucherenko, I. S. ; Ozansoy Kasap, B. ; Akata Kurc, B. ; Dzyadevych, S. V.</creator><creatorcontrib>Pyeshkova, V. N. ; Dudchenko, O. Y. ; Soldatkin, O. O. ; Kucherenko, I. S. ; Ozansoy Kasap, B. ; Akata Kurc, B. ; Dzyadevych, S. V.</creatorcontrib><description>Aim. Improvement of analytical characteristics of an enzyme biosensor based on new inexpensive perspective stainless steel electrodes using silicalite nanoparticles. Methods. Conductometric enzyme biosensor was used. Results. Three methods of glucose oxidase (GOx) immobilization were studied and compared: GOx adsorption on silicalite modified electrodes (GOx-SME); cross-linking by glutaraldehyde without silicalite (GOx-GA); GOx adsorption on SME along with cross-linking by glutaraldehyde (GOx-SME-GA). The GOx-SME-GA biosensors based on stainless steel electrodes were characterized by 12–25-fold higher sensitivity comparing with other biosensors. The developed GOx-SME-GA biosensors were characterized by good reproducibility of glucose biosensors construction (relative standard deviation (RSD) – 18 %), improved signal reproducibility (RSD of glucose determination was 7 %) and good storage stability (29 % loss of activity after 18 days). Conclusions. The method of enzyme immobilization using silicalite together with GA cross-linking sufficiently enhances the enzyme adsorption on the stainless steel electrodes and improves the analytical parameters of biosensors. This method is found to be promising for further creation of other enzyme biosensors.</description><identifier>ISSN: 0233-7657</identifier><identifier>EISSN: 1993-6842</identifier><identifier>DOI: 10.7124/bc.0008C1</identifier><language>eng</language><publisher>Kiev: Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine</publisher><subject>Adsorption ; Biosensors ; Electrodes ; Enzymes ; Glucose oxidase ; Glutaraldehyde ; Immobilization ; Nanoparticles ; Shelf life ; Stainless steel</subject><ispartof>Biopolimery i kletka, 2014, Vol.30 (6), p.462-468</ispartof><rights>2014. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2071-7c680c240ff53fcdaddc82931a440188621b108c3c5adbabc441cc0402a487b63</citedby><cites>FETCH-LOGICAL-c2071-7c680c240ff53fcdaddc82931a440188621b108c3c5adbabc441cc0402a487b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Pyeshkova, V. N.</creatorcontrib><creatorcontrib>Dudchenko, O. Y.</creatorcontrib><creatorcontrib>Soldatkin, O. O.</creatorcontrib><creatorcontrib>Kucherenko, I. 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The developed GOx-SME-GA biosensors were characterized by good reproducibility of glucose biosensors construction (relative standard deviation (RSD) – 18 %), improved signal reproducibility (RSD of glucose determination was 7 %) and good storage stability (29 % loss of activity after 18 days). Conclusions. The method of enzyme immobilization using silicalite together with GA cross-linking sufficiently enhances the enzyme adsorption on the stainless steel electrodes and improves the analytical parameters of biosensors. This method is found to be promising for further creation of other enzyme biosensors.</description><subject>Adsorption</subject><subject>Biosensors</subject><subject>Electrodes</subject><subject>Enzymes</subject><subject>Glucose oxidase</subject><subject>Glutaraldehyde</subject><subject>Immobilization</subject><subject>Nanoparticles</subject><subject>Shelf life</subject><subject>Stainless steel</subject><issn>0233-7657</issn><issn>1993-6842</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNotUMtOwzAQtBBIlMKBP7DEiUPK-tHEOVYVL6kSFzgbZ7MRrpI42ClS-XpShdPOamZnR8PYrYBVIaR-qHAFAGYrzthClKXKcqPlOVuAVCor8nVxya5S2gPk2ki5YJ-bYWg9utGHnoeGJ3_aWj8Sb0Lkvhti-KGO-vHEUv977Ii7OoU4zCc9H7-Ip9H5vqWUJkTUcmoJxxhqStfsonFtopv_uWQfT4_v25ds9_b8ut3sMpRQiKzA3ABKDU2zVg3Wrq7RyFIJpzUIY3IpKgEGFa5dXbkKtRaIoEE6bYoqV0t2N_tOgb8PlEa7D4fYTy-tVJBLU0otJ9X9rMIYUorU2CH6zsWjFWBPBdoK7Vyg-gMwf2Qk</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Pyeshkova, V. 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S. ; Ozansoy Kasap, B. ; Akata Kurc, B. ; Dzyadevych, S. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2071-7c680c240ff53fcdaddc82931a440188621b108c3c5adbabc441cc0402a487b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adsorption</topic><topic>Biosensors</topic><topic>Electrodes</topic><topic>Enzymes</topic><topic>Glucose oxidase</topic><topic>Glutaraldehyde</topic><topic>Immobilization</topic><topic>Nanoparticles</topic><topic>Shelf life</topic><topic>Stainless steel</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pyeshkova, V. N.</creatorcontrib><creatorcontrib>Dudchenko, O. Y.</creatorcontrib><creatorcontrib>Soldatkin, O. O.</creatorcontrib><creatorcontrib>Kucherenko, I. 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N.</au><au>Dudchenko, O. Y.</au><au>Soldatkin, O. O.</au><au>Kucherenko, I. S.</au><au>Ozansoy Kasap, B.</au><au>Akata Kurc, B.</au><au>Dzyadevych, S. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of silicalite for improvement of enzyme adsorption on the stainless steel electrodes</atitle><jtitle>Biopolimery i kletka</jtitle><date>2014</date><risdate>2014</risdate><volume>30</volume><issue>6</issue><spage>462</spage><epage>468</epage><pages>462-468</pages><issn>0233-7657</issn><eissn>1993-6842</eissn><abstract>Aim. Improvement of analytical characteristics of an enzyme biosensor based on new inexpensive perspective stainless steel electrodes using silicalite nanoparticles. Methods. Conductometric enzyme biosensor was used. Results. Three methods of glucose oxidase (GOx) immobilization were studied and compared: GOx adsorption on silicalite modified electrodes (GOx-SME); cross-linking by glutaraldehyde without silicalite (GOx-GA); GOx adsorption on SME along with cross-linking by glutaraldehyde (GOx-SME-GA). The GOx-SME-GA biosensors based on stainless steel electrodes were characterized by 12–25-fold higher sensitivity comparing with other biosensors. The developed GOx-SME-GA biosensors were characterized by good reproducibility of glucose biosensors construction (relative standard deviation (RSD) – 18 %), improved signal reproducibility (RSD of glucose determination was 7 %) and good storage stability (29 % loss of activity after 18 days). Conclusions. The method of enzyme immobilization using silicalite together with GA cross-linking sufficiently enhances the enzyme adsorption on the stainless steel electrodes and improves the analytical parameters of biosensors. 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| subjects | Adsorption Biosensors Electrodes Enzymes Glucose oxidase Glutaraldehyde Immobilization Nanoparticles Shelf life Stainless steel |
| title | Application of silicalite for improvement of enzyme adsorption on the stainless steel electrodes |
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