Extracellular ATP can activate autonomic signal transduction pathways in cultured equine sweat gland epithelial cells
Changes in intracellular free calcium concentration ([Ca2+]i) were monitored in a cell line that was derived from the equine sweat gland epithelium. ATP and closely related compounds could increase [Ca2+]i with a rank order of potency of UTP > or = ATP > ADP >> AMP = adenosine = alpha,be...
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| Veröffentlicht in: | Journal of experimental biology 1994-05, Vol.190 (1), p.239-252 |
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| container_title | Journal of experimental biology |
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| creator | WING HUNG KO O'DOWD, J. J. M PEDIANI, J. D BOVELL, D. L ELDER, H. Y MCEWAN JENKINSON, D WILSON, S. M |
| description | Changes in intracellular free calcium concentration ([Ca2+]i) were monitored in a cell line that was derived from the equine sweat gland epithelium. ATP and closely related compounds could increase [Ca2+]i with a rank order of potency of UTP > or = ATP > ADP >> AMP = adenosine = alpha,beta-methylene-ATP. The responses to ATP and to UTP were initiated by the release of calcium from an internal store and subsequently sustained by calcium influx. The rise in [Ca2+]i thus seems to be mediated by P2U receptors that are coupled to phosphoinositidase C. Some desensitisation of this response developed during repeated stimulation with ATP and this was blocked by staurosporine, an inhibitor of protein kinase C, and augmented by a phorbol ester which acts as an exogenous activator of this enzyme. A protein-kinase-C-dependent inhibitory pathway thus seems to become active during repeated stimulation with ATP. ATP and related compounds could also raise cellular cyclic AMP content. The order of potency was ATP > ADP = AMP = adenosine >> UTP, suggesting that this response is mediated via a separate subclass of P2 receptor. The present results demonstrate that ATP can activate autonomic signal-transduction pathways in cultured equine sweat gland cells and suggest that there may be a purinergic component to the control of secretory activity in the equine sweat gland. |
| doi_str_mv | 10.1242/jeb.190.1.239 |
| format | Article |
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J. M ; PEDIANI, J. D ; BOVELL, D. L ; ELDER, H. Y ; MCEWAN JENKINSON, D ; WILSON, S. M</creator><creatorcontrib>WING HUNG KO ; O'DOWD, J. J. M ; PEDIANI, J. D ; BOVELL, D. L ; ELDER, H. Y ; MCEWAN JENKINSON, D ; WILSON, S. M</creatorcontrib><description>Changes in intracellular free calcium concentration ([Ca2+]i) were monitored in a cell line that was derived from the equine sweat gland epithelium. ATP and closely related compounds could increase [Ca2+]i with a rank order of potency of UTP > or = ATP > ADP >> AMP = adenosine = alpha,beta-methylene-ATP. The responses to ATP and to UTP were initiated by the release of calcium from an internal store and subsequently sustained by calcium influx. The rise in [Ca2+]i thus seems to be mediated by P2U receptors that are coupled to phosphoinositidase C. Some desensitisation of this response developed during repeated stimulation with ATP and this was blocked by staurosporine, an inhibitor of protein kinase C, and augmented by a phorbol ester which acts as an exogenous activator of this enzyme. A protein-kinase-C-dependent inhibitory pathway thus seems to become active during repeated stimulation with ATP. ATP and related compounds could also raise cellular cyclic AMP content. The order of potency was ATP > ADP = AMP = adenosine >> UTP, suggesting that this response is mediated via a separate subclass of P2 receptor. The present results demonstrate that ATP can activate autonomic signal-transduction pathways in cultured equine sweat gland cells and suggest that there may be a purinergic component to the control of secretory activity in the equine sweat gland.</description><identifier>ISSN: 0022-0949</identifier><identifier>EISSN: 1477-9145</identifier><identifier>DOI: 10.1242/jeb.190.1.239</identifier><identifier>PMID: 7964393</identifier><identifier>CODEN: JEBIAM</identifier><language>eng</language><publisher>Cambridge: Company of Biologists</publisher><subject>Adenosine Triphosphate - pharmacology ; Alkaloids - pharmacology ; Animals ; Biological and medical sciences ; Calcium - metabolism ; Cell Line ; Cell physiology ; Cells, Cultured ; Cellular biology ; Cyclic AMP - metabolism ; Epithelium - metabolism ; Fundamental and applied biological sciences. Psychology ; Horses ; Horses - physiology ; Molecular and cellular biology ; Phorbol Esters - pharmacology ; Phosphoric Diester Hydrolases - metabolism ; Receptors, Purinergic - metabolism ; Signal transduction ; Signal Transduction - drug effects ; Staurosporine ; Sweat Glands - cytology ; Sweat Glands - metabolism</subject><ispartof>Journal of experimental biology, 1994-05, Vol.190 (1), p.239-252</ispartof><rights>1994 INIST-CNRS</rights><rights>Copyright Company of Biologists Limited, Department of Zoology May 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-e1bd381dff84bd799f17f9a94a829c75c959c5db4d00bdade92a4094ef066c503</citedby><cites>FETCH-LOGICAL-c419t-e1bd381dff84bd799f17f9a94a829c75c959c5db4d00bdade92a4094ef066c503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3665,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4071551$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7964393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WING HUNG KO</creatorcontrib><creatorcontrib>O'DOWD, J. 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The present results demonstrate that ATP can activate autonomic signal-transduction pathways in cultured equine sweat gland cells and suggest that there may be a purinergic component to the control of secretory activity in the equine sweat gland.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Alkaloids - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Cyclic AMP - metabolism</subject><subject>Epithelium - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Horses</subject><subject>Horses - physiology</subject><subject>Molecular and cellular biology</subject><subject>Phorbol Esters - pharmacology</subject><subject>Phosphoric Diester Hydrolases - metabolism</subject><subject>Receptors, Purinergic - metabolism</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>Staurosporine</subject><subject>Sweat Glands - cytology</subject><subject>Sweat Glands - metabolism</subject><issn>0022-0949</issn><issn>1477-9145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEQhoMotVaPHoUgXrcmm2y3cxSpHyDoQc_LbJKtKeluzYe1_95Ii7mE5H1mhnkIueRsyktZ3q5MO-WQH9NSwBEZc1nXBXBZHZMxY2VZMJBwSs5CWLF8ZpUckVENMylAjEla_ESPyjiXHHp69_5GFfYUVbTfGA3FFId-WFtFg1326Gim-6BTzoeebjB-bnEXqO2pSi4mbzQ1X8n2hoatwUiXDvv8tbHx0zib6_9GhXNy0qEL5uJwT8jHw-L9_ql4eX18vr97KZTkEAvDWy3mXHfdXLa6Buh43QGCxHkJqq4UVKAq3UrNWKtRGyhR5nVNx2YzVTExIdf7vhs_fCUTYrMaks9rhKYUTPCqkiJDxR5SfgjBm67ZeLtGv2s4a_4UN1lxkxU3PFdB5q8OTVO7NvqfPjjN-c0hx6DQdVmYsuEfk6zOc7n4BY1NhnM</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>WING HUNG KO</creator><creator>O'DOWD, J. 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Psychology</topic><topic>Horses</topic><topic>Horses - physiology</topic><topic>Molecular and cellular biology</topic><topic>Phorbol Esters - pharmacology</topic><topic>Phosphoric Diester Hydrolases - metabolism</topic><topic>Receptors, Purinergic - metabolism</topic><topic>Signal transduction</topic><topic>Signal Transduction - drug effects</topic><topic>Staurosporine</topic><topic>Sweat Glands - cytology</topic><topic>Sweat Glands - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WING HUNG KO</creatorcontrib><creatorcontrib>O'DOWD, J. J. M</creatorcontrib><creatorcontrib>PEDIANI, J. D</creatorcontrib><creatorcontrib>BOVELL, D. L</creatorcontrib><creatorcontrib>ELDER, H. Y</creatorcontrib><creatorcontrib>MCEWAN JENKINSON, D</creatorcontrib><creatorcontrib>WILSON, S. 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J. M</au><au>PEDIANI, J. D</au><au>BOVELL, D. L</au><au>ELDER, H. Y</au><au>MCEWAN JENKINSON, D</au><au>WILSON, S. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extracellular ATP can activate autonomic signal transduction pathways in cultured equine sweat gland epithelial cells</atitle><jtitle>Journal of experimental biology</jtitle><addtitle>J Exp Biol</addtitle><date>1994-05-01</date><risdate>1994</risdate><volume>190</volume><issue>1</issue><spage>239</spage><epage>252</epage><pages>239-252</pages><issn>0022-0949</issn><eissn>1477-9145</eissn><coden>JEBIAM</coden><abstract>Changes in intracellular free calcium concentration ([Ca2+]i) were monitored in a cell line that was derived from the equine sweat gland epithelium. ATP and closely related compounds could increase [Ca2+]i with a rank order of potency of UTP > or = ATP > ADP >> AMP = adenosine = alpha,beta-methylene-ATP. The responses to ATP and to UTP were initiated by the release of calcium from an internal store and subsequently sustained by calcium influx. The rise in [Ca2+]i thus seems to be mediated by P2U receptors that are coupled to phosphoinositidase C. Some desensitisation of this response developed during repeated stimulation with ATP and this was blocked by staurosporine, an inhibitor of protein kinase C, and augmented by a phorbol ester which acts as an exogenous activator of this enzyme. A protein-kinase-C-dependent inhibitory pathway thus seems to become active during repeated stimulation with ATP. ATP and related compounds could also raise cellular cyclic AMP content. The order of potency was ATP > ADP = AMP = adenosine >> UTP, suggesting that this response is mediated via a separate subclass of P2 receptor. 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| ispartof | Journal of experimental biology, 1994-05, Vol.190 (1), p.239-252 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Company of Biologists |
| subjects | Adenosine Triphosphate - pharmacology Alkaloids - pharmacology Animals Biological and medical sciences Calcium - metabolism Cell Line Cell physiology Cells, Cultured Cellular biology Cyclic AMP - metabolism Epithelium - metabolism Fundamental and applied biological sciences. Psychology Horses Horses - physiology Molecular and cellular biology Phorbol Esters - pharmacology Phosphoric Diester Hydrolases - metabolism Receptors, Purinergic - metabolism Signal transduction Signal Transduction - drug effects Staurosporine Sweat Glands - cytology Sweat Glands - metabolism |
| title | Extracellular ATP can activate autonomic signal transduction pathways in cultured equine sweat gland epithelial cells |
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