A colorimetric method to measure oxidized, reduced and total glutathione levels in erythrocytes
The most important antioxidant for erythrocytes is glutathione. In this study, a non-enzymatic recycling spectrophotometric method was developed to measure oxidized and reduced glutathione (GSH) levels in erythrocytes. The newly developed method consists of two steps. In the first step, the levels o...
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| Veröffentlicht in: | Journal of laboratory medicine 2019, Vol.43 (5), p.269-277 |
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| creator | Alisik, Murat Neselioglu, Salim Erel, Ozcan |
| description | The most important antioxidant for erythrocytes is glutathione. In this study, a non-enzymatic recycling spectrophotometric method was developed to measure oxidized and reduced glutathione (GSH) levels in erythrocytes. The newly developed method consists of two steps. In the first step, the levels of GSH were determined in the sample. Subsequently, total glutathione levels were measured by reducing the oxidized glutathione (GSSG) with sodium borohydride (NaBH4). Half of the difference between total glutathione and GSH gives the GSSG.The new method was linear between 0 and 3000 μmol/L (r2 = 0.999). The percentage recovery measured by the developed method was 100.2 ± 2.4%. The total precision of the total glutathione, GSH and GSSG was 1.26%, 1.02% and 6.65%, respectively. GSH levels were significantly lower in patients with type 2 diabetes mellitus (DM), while GSSG levels were significantly higher (p |
| doi_str_mv | 10.1515/labmed-2019-0098 |
| format | Article |
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In this study, a non-enzymatic recycling spectrophotometric method was developed to measure oxidized and reduced glutathione (GSH) levels in erythrocytes. The newly developed method consists of two steps. In the first step, the levels of GSH were determined in the sample. Subsequently, total glutathione levels were measured by reducing the oxidized glutathione (GSSG) with sodium borohydride (NaBH4). Half of the difference between total glutathione and GSH gives the GSSG.The new method was linear between 0 and 3000 μmol/L (r2 = 0.999). The percentage recovery measured by the developed method was 100.2 ± 2.4%. The total precision of the total glutathione, GSH and GSSG was 1.26%, 1.02% and 6.65%, respectively. GSH levels were significantly lower in patients with type 2 diabetes mellitus (DM), while GSSG levels were significantly higher (p < 0.05). Hemoglobin A1c (HbA1c) levels were negatively correlated with GSH levels, whereas positively correlated with GSSG (p < 0.05).In conclusion, this method is easy to apply in routine practice with high accuracy, precision and reproducibility.</description><identifier>ISSN: 0342-3026</identifier><identifier>ISSN: 2567-9430</identifier><identifier>EISSN: 1439-0477</identifier><identifier>EISSN: 2567-9449</identifier><identifier>DOI: 10.1515/labmed-2019-0098</identifier><language>eng</language><publisher>Berlin: De Gruyter</publisher><subject>antioxidant ; Antioxidants ; Colorimetry ; Diabetes mellitus ; Diabetes mellitus (non-insulin dependent) ; erythrocyte ; Erythrocytes ; Glutathione ; Hemoglobin ; method ; oxidation ; oxidized glutathione (GSSG) ; reduced glutathione (GSH) ; Sodium ; Spectrophotometry</subject><ispartof>Journal of laboratory medicine, 2019, Vol.43 (5), p.269-277</ispartof><rights>2019. 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Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-adbf898d7ddd4fc048e8f97db0564d9e8f88d60dcb8af810b57147f6ddf20cf63</citedby><cites>FETCH-LOGICAL-c405t-adbf898d7ddd4fc048e8f97db0564d9e8f88d60dcb8af810b57147f6ddf20cf63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Alisik, Murat</creatorcontrib><creatorcontrib>Neselioglu, Salim</creatorcontrib><creatorcontrib>Erel, Ozcan</creatorcontrib><title>A colorimetric method to measure oxidized, reduced and total glutathione levels in erythrocytes</title><title>Journal of laboratory medicine</title><description>The most important antioxidant for erythrocytes is glutathione. In this study, a non-enzymatic recycling spectrophotometric method was developed to measure oxidized and reduced glutathione (GSH) levels in erythrocytes. The newly developed method consists of two steps. In the first step, the levels of GSH were determined in the sample. Subsequently, total glutathione levels were measured by reducing the oxidized glutathione (GSSG) with sodium borohydride (NaBH4). Half of the difference between total glutathione and GSH gives the GSSG.The new method was linear between 0 and 3000 μmol/L (r2 = 0.999). The percentage recovery measured by the developed method was 100.2 ± 2.4%. The total precision of the total glutathione, GSH and GSSG was 1.26%, 1.02% and 6.65%, respectively. GSH levels were significantly lower in patients with type 2 diabetes mellitus (DM), while GSSG levels were significantly higher (p < 0.05). Hemoglobin A1c (HbA1c) levels were negatively correlated with GSH levels, whereas positively correlated with GSSG (p < 0.05).In conclusion, this method is easy to apply in routine practice with high accuracy, precision and reproducibility.</description><subject>antioxidant</subject><subject>Antioxidants</subject><subject>Colorimetry</subject><subject>Diabetes mellitus</subject><subject>Diabetes mellitus (non-insulin dependent)</subject><subject>erythrocyte</subject><subject>Erythrocytes</subject><subject>Glutathione</subject><subject>Hemoglobin</subject><subject>method</subject><subject>oxidation</subject><subject>oxidized glutathione (GSSG)</subject><subject>reduced glutathione (GSH)</subject><subject>Sodium</subject><subject>Spectrophotometry</subject><issn>0342-3026</issn><issn>2567-9430</issn><issn>1439-0477</issn><issn>2567-9449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kEFr3DAQhUVpodu09x4FvcbNSJZt-RJYliYNBHJJz0LWjHa9OFYiyUm3v75aNpBTTm8G3nvDfIx9F_BTNKK5mOzwQFhJEH0F0OsPbCVUXWbVdR_ZCmolqxpk-5l9SWkPILpewoqZNXdhCnF8oBxHx4vsAvIcymTTEomHvyOO_wjPeSRcHCG389GQ7cS305Jt3o1hJj7RM02JjzOneMi7GNwhU_rKPnk7Jfr2qmfsz9Wv-83v6vbu-mazvq2cgiZXFgeve40dIirvQGnSvu9wgKZV2JdFa2wB3aCt1wKGphOq8y2il-B8W5-xH6fexxieFkrZ7MMS53LSyLowqWUrobjg5HIxpBTJm8fyuY0HI8AcMZoTRnPEaI4YS-TyFHmxU6aItI1LeSy-9b8XVXUj277-D1tQffU</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>Alisik, Murat</creator><creator>Neselioglu, Salim</creator><creator>Erel, Ozcan</creator><general>De Gruyter</general><general>Walter de Gruyter GmbH</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>2019</creationdate><title>A colorimetric method to measure oxidized, reduced and total glutathione levels in erythrocytes</title><author>Alisik, Murat ; Neselioglu, Salim ; Erel, Ozcan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-adbf898d7ddd4fc048e8f97db0564d9e8f88d60dcb8af810b57147f6ddf20cf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>antioxidant</topic><topic>Antioxidants</topic><topic>Colorimetry</topic><topic>Diabetes mellitus</topic><topic>Diabetes mellitus (non-insulin dependent)</topic><topic>erythrocyte</topic><topic>Erythrocytes</topic><topic>Glutathione</topic><topic>Hemoglobin</topic><topic>method</topic><topic>oxidation</topic><topic>oxidized glutathione (GSSG)</topic><topic>reduced glutathione (GSH)</topic><topic>Sodium</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alisik, Murat</creatorcontrib><creatorcontrib>Neselioglu, Salim</creatorcontrib><creatorcontrib>Erel, Ozcan</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alisik, Murat</au><au>Neselioglu, Salim</au><au>Erel, Ozcan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A colorimetric method to measure oxidized, reduced and total glutathione levels in erythrocytes</atitle><jtitle>Journal of laboratory medicine</jtitle><date>2019</date><risdate>2019</risdate><volume>43</volume><issue>5</issue><spage>269</spage><epage>277</epage><pages>269-277</pages><issn>0342-3026</issn><issn>2567-9430</issn><eissn>1439-0477</eissn><eissn>2567-9449</eissn><abstract>The most important antioxidant for erythrocytes is glutathione. In this study, a non-enzymatic recycling spectrophotometric method was developed to measure oxidized and reduced glutathione (GSH) levels in erythrocytes. The newly developed method consists of two steps. In the first step, the levels of GSH were determined in the sample. Subsequently, total glutathione levels were measured by reducing the oxidized glutathione (GSSG) with sodium borohydride (NaBH4). Half of the difference between total glutathione and GSH gives the GSSG.The new method was linear between 0 and 3000 μmol/L (r2 = 0.999). The percentage recovery measured by the developed method was 100.2 ± 2.4%. The total precision of the total glutathione, GSH and GSSG was 1.26%, 1.02% and 6.65%, respectively. GSH levels were significantly lower in patients with type 2 diabetes mellitus (DM), while GSSG levels were significantly higher (p < 0.05). Hemoglobin A1c (HbA1c) levels were negatively correlated with GSH levels, whereas positively correlated with GSSG (p < 0.05).In conclusion, this method is easy to apply in routine practice with high accuracy, precision and reproducibility.</abstract><cop>Berlin</cop><pub>De Gruyter</pub><doi>10.1515/labmed-2019-0098</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Walter De Gruyter: Open Access Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | antioxidant Antioxidants Colorimetry Diabetes mellitus Diabetes mellitus (non-insulin dependent) erythrocyte Erythrocytes Glutathione Hemoglobin method oxidation oxidized glutathione (GSSG) reduced glutathione (GSH) Sodium Spectrophotometry |
| title | A colorimetric method to measure oxidized, reduced and total glutathione levels in erythrocytes |
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