Evidence for a functional calcium-sensing receptor that modulates myogenic tone in rat subcutaneous small arteries
Myogenic tone of small arteries is dependent on the presence of extracellular calcium (), and, recently, a receptor that senses changes in Ca2+, the calcium-sensing receptor (CaR), has been detected in vascular tissue. We investigated whether the CaR is involved in the regulation of myogenic tone in...
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| Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 2005-04, Vol.57 (4), p.H1756-H1762 |
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| container_title | American journal of physiology. Heart and circulatory physiology |
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| creator | OHANIAN, Jacqueline GATFIELD, Kelly M WARD, Donald T OHANIAN, Vasken |
| description | Myogenic tone of small arteries is dependent on the presence of extracellular calcium (), and, recently, a receptor that senses changes in Ca2+, the calcium-sensing receptor (CaR), has been detected in vascular tissue. We investigated whether the CaR is involved in the regulation of myogenic tone in rat subcutaneous small arteries. Immunoblot analysis using a monoclonal antibody against the CaR demonstrated its presence in rat subcutaneous arteries. To determine whether the CaR was functionally active, segments of artery ( |
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We investigated whether the CaR is involved in the regulation of myogenic tone in rat subcutaneous small arteries. Immunoblot analysis using a monoclonal antibody against the CaR demonstrated its presence in rat subcutaneous arteries. To determine whether the CaR was functionally active, segments of artery (<250 mm internal diameter) mounted in a pressure myograph with an intraluminal pressure of 70 mmHg were studied after the development of myogenic tone. Increasing concentration ([Ca2+]o) cumulatively from 0.5 to 10 mM induced an initial constriction (0.5-2 mM) followed by dilation (42 plus or minus 5% loss of tone). The dose-dependent dilation was mimicked by other known CaR agonists including magnesium (1-10 mM) and the aminoglycosides neomycin (0.003-10 mM) and kanamycin (0.003-3 mM). PKC activation with the phorbol ester phorbol-12,13-dibutyrate (20nM) inhibited the dilation induced by high [Ca2+]o or neomycin, whereas inhibition of PKC with GF109203X (10 mM) increased the responses to or neomycin, consistent with the role of PKC as a negative regulator of the CaR. We conclude that rat subcutaneous arteries express a functionally active CaR that may be involved in the modulation of myogenic tone and hence the regulation of peripheral vascular resistance. [PUBLICATION ABSTRACT]</description><identifier>ISSN: 0363-6135</identifier><identifier>EISSN: 1522-1539</identifier><identifier>CODEN: AJPPDI</identifier><language>eng</language><publisher>Bethesda, MD: American Physiological Society</publisher><subject>Biological and medical sciences ; Blood vessels ; Calcium ; Fundamental and applied biological sciences. Psychology ; Magnesium ; Muscular system ; Rodents ; Vertebrates: cardiovascular system</subject><ispartof>American journal of physiology. Heart and circulatory physiology, 2005-04, Vol.57 (4), p.H1756-H1762</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright American Physiological Society Apr 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16610227$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>OHANIAN, Jacqueline</creatorcontrib><creatorcontrib>GATFIELD, Kelly M</creatorcontrib><creatorcontrib>WARD, Donald T</creatorcontrib><creatorcontrib>OHANIAN, Vasken</creatorcontrib><title>Evidence for a functional calcium-sensing receptor that modulates myogenic tone in rat subcutaneous small arteries</title><title>American journal of physiology. Heart and circulatory physiology</title><description>Myogenic tone of small arteries is dependent on the presence of extracellular calcium (), and, recently, a receptor that senses changes in Ca2+, the calcium-sensing receptor (CaR), has been detected in vascular tissue. We investigated whether the CaR is involved in the regulation of myogenic tone in rat subcutaneous small arteries. Immunoblot analysis using a monoclonal antibody against the CaR demonstrated its presence in rat subcutaneous arteries. To determine whether the CaR was functionally active, segments of artery (<250 mm internal diameter) mounted in a pressure myograph with an intraluminal pressure of 70 mmHg were studied after the development of myogenic tone. Increasing concentration ([Ca2+]o) cumulatively from 0.5 to 10 mM induced an initial constriction (0.5-2 mM) followed by dilation (42 plus or minus 5% loss of tone). The dose-dependent dilation was mimicked by other known CaR agonists including magnesium (1-10 mM) and the aminoglycosides neomycin (0.003-10 mM) and kanamycin (0.003-3 mM). PKC activation with the phorbol ester phorbol-12,13-dibutyrate (20nM) inhibited the dilation induced by high [Ca2+]o or neomycin, whereas inhibition of PKC with GF109203X (10 mM) increased the responses to or neomycin, consistent with the role of PKC as a negative regulator of the CaR. We conclude that rat subcutaneous arteries express a functionally active CaR that may be involved in the modulation of myogenic tone and hence the regulation of peripheral vascular resistance. [PUBLICATION ABSTRACT]</description><subject>Biological and medical sciences</subject><subject>Blood vessels</subject><subject>Calcium</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Magnesium</subject><subject>Muscular system</subject><subject>Rodents</subject><subject>Vertebrates: cardiovascular system</subject><issn>0363-6135</issn><issn>1522-1539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNotj0tLxDAURosoOI7-hyC4LOTRJM1ShvEBAy50X27T2zFDm9Q8hPn3FpzVt_gOB85VtWGS85pJYa6rDRVK1IoJeVvdpXSilEqtxKaK-183oLdIxhAJkLF4m13wMBELk3VlrhP65PyRRLS45JXK35DJHIYyQcZE5nM4oneW5OCROE_ieqfS25LBYyiJpBmmiUDMGB2m--pmhCnhw2W31efL_mv3Vh8-Xt93z4d6kbqpYWwHIxCgtRpaqmxrNOWCoeDCtJz3XHHdMEm1HqziRlBheW90P6BpsBXb6vHfusTwUzDl7hRKXLtSx7lRTCrVrNDTBYK05o4RvHWpW6KbIZ47phSjnGvxBz51ZLo</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>OHANIAN, Jacqueline</creator><creator>GATFIELD, Kelly M</creator><creator>WARD, Donald T</creator><creator>OHANIAN, Vasken</creator><general>American Physiological Society</general><scope>IQODW</scope><scope>7QP</scope><scope>7QR</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050401</creationdate><title>Evidence for a functional calcium-sensing receptor that modulates myogenic tone in rat subcutaneous small arteries</title><author>OHANIAN, Jacqueline ; GATFIELD, Kelly M ; WARD, Donald T ; OHANIAN, Vasken</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p574-af8d93eaa8c7a806c8970231e3239822b2627415077dc629303c2b97bde94e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biological and medical sciences</topic><topic>Blood vessels</topic><topic>Calcium</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Magnesium</topic><topic>Muscular system</topic><topic>Rodents</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OHANIAN, Jacqueline</creatorcontrib><creatorcontrib>GATFIELD, Kelly M</creatorcontrib><creatorcontrib>WARD, Donald T</creatorcontrib><creatorcontrib>OHANIAN, Vasken</creatorcontrib><collection>Pascal-Francis</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OHANIAN, Jacqueline</au><au>GATFIELD, Kelly M</au><au>WARD, Donald T</au><au>OHANIAN, Vasken</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for a functional calcium-sensing receptor that modulates myogenic tone in rat subcutaneous small arteries</atitle><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle><date>2005-04-01</date><risdate>2005</risdate><volume>57</volume><issue>4</issue><spage>H1756</spage><epage>H1762</epage><pages>H1756-H1762</pages><issn>0363-6135</issn><eissn>1522-1539</eissn><coden>AJPPDI</coden><abstract>Myogenic tone of small arteries is dependent on the presence of extracellular calcium (), and, recently, a receptor that senses changes in Ca2+, the calcium-sensing receptor (CaR), has been detected in vascular tissue. We investigated whether the CaR is involved in the regulation of myogenic tone in rat subcutaneous small arteries. Immunoblot analysis using a monoclonal antibody against the CaR demonstrated its presence in rat subcutaneous arteries. To determine whether the CaR was functionally active, segments of artery (<250 mm internal diameter) mounted in a pressure myograph with an intraluminal pressure of 70 mmHg were studied after the development of myogenic tone. Increasing concentration ([Ca2+]o) cumulatively from 0.5 to 10 mM induced an initial constriction (0.5-2 mM) followed by dilation (42 plus or minus 5% loss of tone). The dose-dependent dilation was mimicked by other known CaR agonists including magnesium (1-10 mM) and the aminoglycosides neomycin (0.003-10 mM) and kanamycin (0.003-3 mM). PKC activation with the phorbol ester phorbol-12,13-dibutyrate (20nM) inhibited the dilation induced by high [Ca2+]o or neomycin, whereas inhibition of PKC with GF109203X (10 mM) increased the responses to or neomycin, consistent with the role of PKC as a negative regulator of the CaR. We conclude that rat subcutaneous arteries express a functionally active CaR that may be involved in the modulation of myogenic tone and hence the regulation of peripheral vascular resistance. [PUBLICATION ABSTRACT]</abstract><cop>Bethesda, MD</cop><pub>American Physiological Society</pub></addata></record> |
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| ispartof | American journal of physiology. Heart and circulatory physiology, 2005-04, Vol.57 (4), p.H1756-H1762 |
| issn | 0363-6135 1522-1539 |
| language | eng |
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| source | American Physiological Society; EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Blood vessels Calcium Fundamental and applied biological sciences. Psychology Magnesium Muscular system Rodents Vertebrates: cardiovascular system |
| title | Evidence for a functional calcium-sensing receptor that modulates myogenic tone in rat subcutaneous small arteries |
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