Antimicrobial activity of Luma apiculata against resistant strains of Staphylococcus aureus and Klebsiella pneumoniae
Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents. Studies have reported an antimicrobial action of Luma apiculata, however, there are no in vitro studies demonstrating the effect of this plant against multidrug-resistant bacteria. The o...
Gespeichert in:
| Veröffentlicht in: | Pure and applied biology 2019-09, Vol.8 (3), p.1962-1974 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1974 |
|---|---|
| container_issue | 3 |
| container_start_page | 1962 |
| container_title | Pure and applied biology |
| container_volume | 8 |
| creator | Treulen, Favián F |
| description | Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents. Studies have reported an antimicrobial action of Luma apiculata, however, there are no in vitro studies demonstrating the effect of this plant against multidrug-resistant bacteria. The objective of this study was to explore the antimicrobial properties of alcoholic extract of Luma apiculata against standard multi-resistant bacterial pathogens. Multidrug-resistant strains of Staphylococcus aureus ATCC 43300 (MRSA) and Klebsiella pneumoniae ATCC BAA 1705 (CRKP) were incubated independently for 72 h at 37°C with different concentrations (1-10,000 pg/mL) of alcoholic extract of Luma apiculata. The growth curve was studied up to 72 hours. The minimum inhibitory concentration (MIC) was determined by broth macrodilution method. The agar diffusion method was used to examine bacteriostatic activity and bactericidal activity was evaluated by a commercial kit and analyzed by flow cytometry. Analyses of chromatographic data, as well as mass spectrometry, were performed to determine the main compounds present in the extracts. Luma apiculata leaves extracts showed bactericidal activity and was effective attenuating growth in both bacterial strains. Thus, Luma apiculata could represent a natural alternative against multidrug-resistant bacterial strains. However, further studies should be undertaken to test their clinical utility. |
| doi_str_mv | 10.19045/bspab.2019.80140 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2296009364</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2296009364</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1610-590efa769428321dce57d5a3f24b04978ebe5afac0bd58c3b607c1a8283251d3</originalsourceid><addsrcrecordid>eNpNkMlKA0EQhhtRMMQ8gLcGzxN7neUYgksw4MHcm-qeHu0wm70IeXtnEg_W5f8pPqrgQ-iekjWtiJCPOoyg14zQal0SKsgVWjBORMZEUV7_67doFcKRTFMVnBRygdKmj65zxg_aQYvBRPfj4gkPDd6nDjCMzqQW4tQ-wfUhYm-DCxH6iEP082pmPyKMX6d2MIMxKWBI3s7R1_ittTo427aAx96mbugd2Dt000Ab7Oovl-jw_HTYvmb795fddrPPDM0pyWRFbANFXglWckZrY2VRS-ANE5qIqiitthIaMETXsjRc56QwFMqZlrTmS_RwOTv64TvZENVxSL6fPirGqnyywHMxUfRCTRJC8LZRo3cd-JOiRJ39qrNfNftVZ7_8F1l3cRw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2296009364</pqid></control><display><type>article</type><title>Antimicrobial activity of Luma apiculata against resistant strains of Staphylococcus aureus and Klebsiella pneumoniae</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Treulen, Favián F</creator><creatorcontrib>Treulen, Favián F</creatorcontrib><description>Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents. Studies have reported an antimicrobial action of Luma apiculata, however, there are no in vitro studies demonstrating the effect of this plant against multidrug-resistant bacteria. The objective of this study was to explore the antimicrobial properties of alcoholic extract of Luma apiculata against standard multi-resistant bacterial pathogens. Multidrug-resistant strains of Staphylococcus aureus ATCC 43300 (MRSA) and Klebsiella pneumoniae ATCC BAA 1705 (CRKP) were incubated independently for 72 h at 37°C with different concentrations (1-10,000 pg/mL) of alcoholic extract of Luma apiculata. The growth curve was studied up to 72 hours. The minimum inhibitory concentration (MIC) was determined by broth macrodilution method. The agar diffusion method was used to examine bacteriostatic activity and bactericidal activity was evaluated by a commercial kit and analyzed by flow cytometry. Analyses of chromatographic data, as well as mass spectrometry, were performed to determine the main compounds present in the extracts. Luma apiculata leaves extracts showed bactericidal activity and was effective attenuating growth in both bacterial strains. Thus, Luma apiculata could represent a natural alternative against multidrug-resistant bacterial strains. However, further studies should be undertaken to test their clinical utility.</description><identifier>ISSN: 2304-2478</identifier><identifier>EISSN: 2304-2478</identifier><identifier>DOI: 10.19045/bspab.2019.80140</identifier><language>eng</language><publisher>Quetta: International Society of Pure and Applied Biology</publisher><subject>Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial activity ; Antimicrobial agents ; Apoptosis ; Bacteria ; Bactericidal activity ; Drug resistance ; E coli ; Flavonoids ; Flow cytometry ; Herbal medicine ; Kinases ; Klebsiella ; Klebsiella pneumoniae ; Mass spectrometry ; Mass spectroscopy ; Minimum inhibitory concentration ; Multidrug resistance ; Multidrug resistant organisms ; Nosocomial infections ; Oils & fats ; Pathogens ; Staphylococcus aureus ; Staphylococcus infections ; Strains (organisms) ; Studies</subject><ispartof>Pure and applied biology, 2019-09, Vol.8 (3), p.1962-1974</ispartof><rights>2019. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1610-590efa769428321dce57d5a3f24b04978ebe5afac0bd58c3b607c1a8283251d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Treulen, Favián F</creatorcontrib><title>Antimicrobial activity of Luma apiculata against resistant strains of Staphylococcus aureus and Klebsiella pneumoniae</title><title>Pure and applied biology</title><description>Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents. Studies have reported an antimicrobial action of Luma apiculata, however, there are no in vitro studies demonstrating the effect of this plant against multidrug-resistant bacteria. The objective of this study was to explore the antimicrobial properties of alcoholic extract of Luma apiculata against standard multi-resistant bacterial pathogens. Multidrug-resistant strains of Staphylococcus aureus ATCC 43300 (MRSA) and Klebsiella pneumoniae ATCC BAA 1705 (CRKP) were incubated independently for 72 h at 37°C with different concentrations (1-10,000 pg/mL) of alcoholic extract of Luma apiculata. The growth curve was studied up to 72 hours. The minimum inhibitory concentration (MIC) was determined by broth macrodilution method. The agar diffusion method was used to examine bacteriostatic activity and bactericidal activity was evaluated by a commercial kit and analyzed by flow cytometry. Analyses of chromatographic data, as well as mass spectrometry, were performed to determine the main compounds present in the extracts. Luma apiculata leaves extracts showed bactericidal activity and was effective attenuating growth in both bacterial strains. Thus, Luma apiculata could represent a natural alternative against multidrug-resistant bacterial strains. However, further studies should be undertaken to test their clinical utility.</description><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Apoptosis</subject><subject>Bacteria</subject><subject>Bactericidal activity</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Flavonoids</subject><subject>Flow cytometry</subject><subject>Herbal medicine</subject><subject>Kinases</subject><subject>Klebsiella</subject><subject>Klebsiella pneumoniae</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Minimum inhibitory concentration</subject><subject>Multidrug resistance</subject><subject>Multidrug resistant organisms</subject><subject>Nosocomial infections</subject><subject>Oils & fats</subject><subject>Pathogens</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Strains (organisms)</subject><subject>Studies</subject><issn>2304-2478</issn><issn>2304-2478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpNkMlKA0EQhhtRMMQ8gLcGzxN7neUYgksw4MHcm-qeHu0wm70IeXtnEg_W5f8pPqrgQ-iekjWtiJCPOoyg14zQal0SKsgVWjBORMZEUV7_67doFcKRTFMVnBRygdKmj65zxg_aQYvBRPfj4gkPDd6nDjCMzqQW4tQ-wfUhYm-DCxH6iEP082pmPyKMX6d2MIMxKWBI3s7R1_ittTo427aAx96mbugd2Dt000Ab7Oovl-jw_HTYvmb795fddrPPDM0pyWRFbANFXglWckZrY2VRS-ANE5qIqiitthIaMETXsjRc56QwFMqZlrTmS_RwOTv64TvZENVxSL6fPirGqnyywHMxUfRCTRJC8LZRo3cd-JOiRJ39qrNfNftVZ7_8F1l3cRw</recordid><startdate>20190910</startdate><enddate>20190910</enddate><creator>Treulen, Favián F</creator><general>International Society of Pure and Applied Biology</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20190910</creationdate><title>Antimicrobial activity of Luma apiculata against resistant strains of Staphylococcus aureus and Klebsiella pneumoniae</title><author>Treulen, Favián F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1610-590efa769428321dce57d5a3f24b04978ebe5afac0bd58c3b607c1a8283251d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Apoptosis</topic><topic>Bacteria</topic><topic>Bactericidal activity</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Flavonoids</topic><topic>Flow cytometry</topic><topic>Herbal medicine</topic><topic>Kinases</topic><topic>Klebsiella</topic><topic>Klebsiella pneumoniae</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Minimum inhibitory concentration</topic><topic>Multidrug resistance</topic><topic>Multidrug resistant organisms</topic><topic>Nosocomial infections</topic><topic>Oils & fats</topic><topic>Pathogens</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus infections</topic><topic>Strains (organisms)</topic><topic>Studies</topic><toplevel>online_resources</toplevel><creatorcontrib>Treulen, Favián F</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Pure and applied biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Treulen, Favián F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial activity of Luma apiculata against resistant strains of Staphylococcus aureus and Klebsiella pneumoniae</atitle><jtitle>Pure and applied biology</jtitle><date>2019-09-10</date><risdate>2019</risdate><volume>8</volume><issue>3</issue><spage>1962</spage><epage>1974</epage><pages>1962-1974</pages><issn>2304-2478</issn><eissn>2304-2478</eissn><abstract>Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents. Studies have reported an antimicrobial action of Luma apiculata, however, there are no in vitro studies demonstrating the effect of this plant against multidrug-resistant bacteria. The objective of this study was to explore the antimicrobial properties of alcoholic extract of Luma apiculata against standard multi-resistant bacterial pathogens. Multidrug-resistant strains of Staphylococcus aureus ATCC 43300 (MRSA) and Klebsiella pneumoniae ATCC BAA 1705 (CRKP) were incubated independently for 72 h at 37°C with different concentrations (1-10,000 pg/mL) of alcoholic extract of Luma apiculata. The growth curve was studied up to 72 hours. The minimum inhibitory concentration (MIC) was determined by broth macrodilution method. The agar diffusion method was used to examine bacteriostatic activity and bactericidal activity was evaluated by a commercial kit and analyzed by flow cytometry. Analyses of chromatographic data, as well as mass spectrometry, were performed to determine the main compounds present in the extracts. Luma apiculata leaves extracts showed bactericidal activity and was effective attenuating growth in both bacterial strains. Thus, Luma apiculata could represent a natural alternative against multidrug-resistant bacterial strains. However, further studies should be undertaken to test their clinical utility.</abstract><cop>Quetta</cop><pub>International Society of Pure and Applied Biology</pub><doi>10.19045/bspab.2019.80140</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2304-2478 |
| ispartof | Pure and applied biology, 2019-09, Vol.8 (3), p.1962-1974 |
| issn | 2304-2478 2304-2478 |
| language | eng |
| recordid | cdi_proquest_journals_2296009364 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Antibiotics Antiinfectives and antibacterials Antimicrobial activity Antimicrobial agents Apoptosis Bacteria Bactericidal activity Drug resistance E coli Flavonoids Flow cytometry Herbal medicine Kinases Klebsiella Klebsiella pneumoniae Mass spectrometry Mass spectroscopy Minimum inhibitory concentration Multidrug resistance Multidrug resistant organisms Nosocomial infections Oils & fats Pathogens Staphylococcus aureus Staphylococcus infections Strains (organisms) Studies |
| title | Antimicrobial activity of Luma apiculata against resistant strains of Staphylococcus aureus and Klebsiella pneumoniae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T13%3A18%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antimicrobial%20activity%20of%20Luma%20apiculata%20against%20resistant%20strains%20of%20Staphylococcus%20aureus%20and%20Klebsiella%20pneumoniae&rft.jtitle=Pure%20and%20applied%20biology&rft.au=Treulen,%20Favi%C3%A1n%20F&rft.date=2019-09-10&rft.volume=8&rft.issue=3&rft.spage=1962&rft.epage=1974&rft.pages=1962-1974&rft.issn=2304-2478&rft.eissn=2304-2478&rft_id=info:doi/10.19045/bspab.2019.80140&rft_dat=%3Cproquest_cross%3E2296009364%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2296009364&rft_id=info:pmid/&rfr_iscdi=true |