ChiIV3 Acts as a Novel Target of WRKY40 to Mediate Pepper Immunity Against Ralstonia solanacearum Infection
ChiIV3, a chitinase of pepper ( ), stimulates cell death in pepper plants. However, there are only scarce reports on its role in resistance against bacterial wilt disease such as that caused by and their transcriptional regulation. In this study, the silencing of in pepper plants significantly reduc...
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| Veröffentlicht in: | Molecular plant-microbe interactions 2019-09, Vol.32 (9), p.MPMI11180313R-1133 |
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| creator | Liu, Zhiqin Shi, Lanping Weng, Yahong Zou, Huasong Li, Xia Yang, Sheng Qiu, Shanshan Huang, Xueying Huang, Jinfeng Hussain, Ansar Zhang, Kan Guan, Deyi He, Shuilin |
| description | ChiIV3, a chitinase of pepper (
), stimulates cell death in pepper plants. However, there are only scarce reports on its role in resistance against bacterial wilt disease such as that caused by
and their transcriptional regulation. In this study, the silencing of
in pepper plants significantly reduced the resistance to
. The transcript of
was induced by
inoculation (RSI) as well as exogenous application of methyl jasmonate and abscisic acid. The bioinformatics analysis revealed that the
promoter consists of multiple stress-related
elements, including six W-boxes and one MYB1AT. With the 5' deletion assay in the
promoter, the W4-box located from -640 to -635 bp was identified as the
element that is required for the response to RSI. In addition, the W4-box element was shown to be essential for the binding of the
promoter by the WRKY40 transcription factor, which is known to positively regulate the defense response to
. Site-directed mutagenesis in the W4-box sequence impaired the binding of WRKY40 to the
promoter. Subsequently, the transcription of
decreased in
-silenced pepper plants. These results demonstrated that the expression of the defense gene
is controlled through multiple modes of regulation, and WRKY40 directly binds to the W4-box element of the
promoter region for its transcriptional regulation. |
| doi_str_mv | 10.1094/MPMI-11-18-0313-R |
| format | Article |
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), stimulates cell death in pepper plants. However, there are only scarce reports on its role in resistance against bacterial wilt disease such as that caused by
and their transcriptional regulation. In this study, the silencing of
in pepper plants significantly reduced the resistance to
. The transcript of
was induced by
inoculation (RSI) as well as exogenous application of methyl jasmonate and abscisic acid. The bioinformatics analysis revealed that the
promoter consists of multiple stress-related
elements, including six W-boxes and one MYB1AT. With the 5' deletion assay in the
promoter, the W4-box located from -640 to -635 bp was identified as the
element that is required for the response to RSI. In addition, the W4-box element was shown to be essential for the binding of the
promoter by the WRKY40 transcription factor, which is known to positively regulate the defense response to
. Site-directed mutagenesis in the W4-box sequence impaired the binding of WRKY40 to the
promoter. Subsequently, the transcription of
decreased in
-silenced pepper plants. These results demonstrated that the expression of the defense gene
is controlled through multiple modes of regulation, and WRKY40 directly binds to the W4-box element of the
promoter region for its transcriptional regulation.</description><identifier>ISSN: 0894-0282</identifier><identifier>EISSN: 1943-7706</identifier><identifier>DOI: 10.1094/MPMI-11-18-0313-R</identifier><identifier>PMID: 31039081</identifier><language>eng</language><publisher>United States: American Phytopathological Society</publisher><subject>Abscisic acid ; Binding ; Bioinformatics ; Capsicum annuum ; Cell death ; Chitinase ; Clonal deletion ; Disease resistance ; Gene deletion ; Gene expression ; Gene regulation ; Immunity ; Inoculation ; Methyl jasmonate ; Peppers ; Ralstonia solanacearum ; Site-directed mutagenesis ; Tungsten ; Vegetables ; Wilt</subject><ispartof>Molecular plant-microbe interactions, 2019-09, Vol.32 (9), p.MPMI11180313R-1133</ispartof><rights>Copyright American Phytopathological Society Sep 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-fc2afda2aba96e9b865f83677abf175439eb32c2ed3e79b804de90f504a5e0553</citedby><cites>FETCH-LOGICAL-c372t-fc2afda2aba96e9b865f83677abf175439eb32c2ed3e79b804de90f504a5e0553</cites><orcidid>0000-0001-5758-1975 ; 0000-0001-5032-7560</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31039081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Zhiqin</creatorcontrib><creatorcontrib>Shi, Lanping</creatorcontrib><creatorcontrib>Weng, Yahong</creatorcontrib><creatorcontrib>Zou, Huasong</creatorcontrib><creatorcontrib>Li, Xia</creatorcontrib><creatorcontrib>Yang, Sheng</creatorcontrib><creatorcontrib>Qiu, Shanshan</creatorcontrib><creatorcontrib>Huang, Xueying</creatorcontrib><creatorcontrib>Huang, Jinfeng</creatorcontrib><creatorcontrib>Hussain, Ansar</creatorcontrib><creatorcontrib>Zhang, Kan</creatorcontrib><creatorcontrib>Guan, Deyi</creatorcontrib><creatorcontrib>He, Shuilin</creatorcontrib><title>ChiIV3 Acts as a Novel Target of WRKY40 to Mediate Pepper Immunity Against Ralstonia solanacearum Infection</title><title>Molecular plant-microbe interactions</title><addtitle>Mol Plant Microbe Interact</addtitle><description>ChiIV3, a chitinase of pepper (
), stimulates cell death in pepper plants. However, there are only scarce reports on its role in resistance against bacterial wilt disease such as that caused by
and their transcriptional regulation. In this study, the silencing of
in pepper plants significantly reduced the resistance to
. The transcript of
was induced by
inoculation (RSI) as well as exogenous application of methyl jasmonate and abscisic acid. The bioinformatics analysis revealed that the
promoter consists of multiple stress-related
elements, including six W-boxes and one MYB1AT. With the 5' deletion assay in the
promoter, the W4-box located from -640 to -635 bp was identified as the
element that is required for the response to RSI. In addition, the W4-box element was shown to be essential for the binding of the
promoter by the WRKY40 transcription factor, which is known to positively regulate the defense response to
. Site-directed mutagenesis in the W4-box sequence impaired the binding of WRKY40 to the
promoter. Subsequently, the transcription of
decreased in
-silenced pepper plants. These results demonstrated that the expression of the defense gene
is controlled through multiple modes of regulation, and WRKY40 directly binds to the W4-box element of the
promoter region for its transcriptional regulation.</description><subject>Abscisic acid</subject><subject>Binding</subject><subject>Bioinformatics</subject><subject>Capsicum annuum</subject><subject>Cell death</subject><subject>Chitinase</subject><subject>Clonal deletion</subject><subject>Disease resistance</subject><subject>Gene deletion</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Immunity</subject><subject>Inoculation</subject><subject>Methyl jasmonate</subject><subject>Peppers</subject><subject>Ralstonia solanacearum</subject><subject>Site-directed mutagenesis</subject><subject>Tungsten</subject><subject>Vegetables</subject><subject>Wilt</subject><issn>0894-0282</issn><issn>1943-7706</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo9kFtLJDEQRsOyso66P2BflsA-R3PpdJLHYfDS6KgMXvAppLsrbut0Z0zSgv_eHrxAQT3U-b6Cg9AfRg8ZNcXR8npZEcYI04QKJsjqB5oxUwiiFC1_ohnVpiCUa76L9lJ6opSZUspfaFcwKgzVbIaeF_-76k7geZMTdtPgy_AKa3zj4iNkHDy-X50_FBTngJfQdi4DvobNBiKu-n4cuvyG54-uG1LGK7dOOQydwyms3eAacHHscTV4aHIXhgO04ycEfn_ufXR7cnyzOCMXV6fVYn5BGqF4Jr7hzreOu9qZEkytS-m1KJVytWdKFsJALXjDoRWgpjMtWjDUS1o4CVRKsY_-ffRuYngZIWX7FMY4TC8t51qXShplJop9UE0MKUXwdhO73sU3y6jd6rVbvZYxy7Td6rWrKfP3s3mse2i_E18-xTtLKHWF</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Liu, Zhiqin</creator><creator>Shi, Lanping</creator><creator>Weng, Yahong</creator><creator>Zou, Huasong</creator><creator>Li, Xia</creator><creator>Yang, Sheng</creator><creator>Qiu, Shanshan</creator><creator>Huang, Xueying</creator><creator>Huang, Jinfeng</creator><creator>Hussain, Ansar</creator><creator>Zhang, Kan</creator><creator>Guan, Deyi</creator><creator>He, Shuilin</creator><general>American Phytopathological Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0001-5758-1975</orcidid><orcidid>https://orcid.org/0000-0001-5032-7560</orcidid></search><sort><creationdate>20190901</creationdate><title>ChiIV3 Acts as a Novel Target of WRKY40 to Mediate Pepper Immunity Against Ralstonia solanacearum Infection</title><author>Liu, Zhiqin ; Shi, Lanping ; Weng, Yahong ; Zou, Huasong ; Li, Xia ; Yang, Sheng ; Qiu, Shanshan ; Huang, Xueying ; Huang, Jinfeng ; Hussain, Ansar ; Zhang, Kan ; Guan, Deyi ; He, Shuilin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-fc2afda2aba96e9b865f83677abf175439eb32c2ed3e79b804de90f504a5e0553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abscisic acid</topic><topic>Binding</topic><topic>Bioinformatics</topic><topic>Capsicum annuum</topic><topic>Cell death</topic><topic>Chitinase</topic><topic>Clonal deletion</topic><topic>Disease resistance</topic><topic>Gene deletion</topic><topic>Gene expression</topic><topic>Gene regulation</topic><topic>Immunity</topic><topic>Inoculation</topic><topic>Methyl jasmonate</topic><topic>Peppers</topic><topic>Ralstonia solanacearum</topic><topic>Site-directed mutagenesis</topic><topic>Tungsten</topic><topic>Vegetables</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Zhiqin</creatorcontrib><creatorcontrib>Shi, Lanping</creatorcontrib><creatorcontrib>Weng, Yahong</creatorcontrib><creatorcontrib>Zou, Huasong</creatorcontrib><creatorcontrib>Li, Xia</creatorcontrib><creatorcontrib>Yang, Sheng</creatorcontrib><creatorcontrib>Qiu, Shanshan</creatorcontrib><creatorcontrib>Huang, Xueying</creatorcontrib><creatorcontrib>Huang, Jinfeng</creatorcontrib><creatorcontrib>Hussain, Ansar</creatorcontrib><creatorcontrib>Zhang, Kan</creatorcontrib><creatorcontrib>Guan, Deyi</creatorcontrib><creatorcontrib>He, Shuilin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Molecular plant-microbe interactions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Zhiqin</au><au>Shi, Lanping</au><au>Weng, Yahong</au><au>Zou, Huasong</au><au>Li, Xia</au><au>Yang, Sheng</au><au>Qiu, Shanshan</au><au>Huang, Xueying</au><au>Huang, Jinfeng</au><au>Hussain, Ansar</au><au>Zhang, Kan</au><au>Guan, Deyi</au><au>He, Shuilin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ChiIV3 Acts as a Novel Target of WRKY40 to Mediate Pepper Immunity Against Ralstonia solanacearum Infection</atitle><jtitle>Molecular plant-microbe interactions</jtitle><addtitle>Mol Plant Microbe Interact</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>32</volume><issue>9</issue><spage>MPMI11180313R</spage><epage>1133</epage><pages>MPMI11180313R-1133</pages><issn>0894-0282</issn><eissn>1943-7706</eissn><abstract>ChiIV3, a chitinase of pepper (
), stimulates cell death in pepper plants. However, there are only scarce reports on its role in resistance against bacterial wilt disease such as that caused by
and their transcriptional regulation. In this study, the silencing of
in pepper plants significantly reduced the resistance to
. The transcript of
was induced by
inoculation (RSI) as well as exogenous application of methyl jasmonate and abscisic acid. The bioinformatics analysis revealed that the
promoter consists of multiple stress-related
elements, including six W-boxes and one MYB1AT. With the 5' deletion assay in the
promoter, the W4-box located from -640 to -635 bp was identified as the
element that is required for the response to RSI. In addition, the W4-box element was shown to be essential for the binding of the
promoter by the WRKY40 transcription factor, which is known to positively regulate the defense response to
. Site-directed mutagenesis in the W4-box sequence impaired the binding of WRKY40 to the
promoter. Subsequently, the transcription of
decreased in
-silenced pepper plants. These results demonstrated that the expression of the defense gene
is controlled through multiple modes of regulation, and WRKY40 directly binds to the W4-box element of the
promoter region for its transcriptional regulation.</abstract><cop>United States</cop><pub>American Phytopathological Society</pub><pmid>31039081</pmid><doi>10.1094/MPMI-11-18-0313-R</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-5758-1975</orcidid><orcidid>https://orcid.org/0000-0001-5032-7560</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 0894-0282 1943-7706 |
| language | eng |
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| source | EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Abscisic acid Binding Bioinformatics Capsicum annuum Cell death Chitinase Clonal deletion Disease resistance Gene deletion Gene expression Gene regulation Immunity Inoculation Methyl jasmonate Peppers Ralstonia solanacearum Site-directed mutagenesis Tungsten Vegetables Wilt |
| title | ChiIV3 Acts as a Novel Target of WRKY40 to Mediate Pepper Immunity Against Ralstonia solanacearum Infection |
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