Expression profiles of Br MYB transcription factors related to glucosinolate biosynthesis and stress response in eight subspecies of Brassica rapa
Brassica rapa is a polyploid species with phenotypically diverse cultivated subspecies. Glucosinolates ( GSL s) are secondary metabolites that contribute to anticarcinogenic activity and plant defense in Brassicaceae. Previously, complete coding sequences of 13 Br MYB transcription factors ( TF s) r...
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Veröffentlicht in: | FEBS open bio 2017-11, Vol.7 (11), p.1646-1659 |
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Zusammenfassung: | Brassica rapa
is a polyploid species with phenotypically diverse cultivated subspecies. Glucosinolates (
GSL
s) are secondary metabolites that contribute to anticarcinogenic activity and plant defense in Brassicaceae. Previously, complete coding sequences of 13
Br
MYB
transcription factors (
TF
s) related to
GSL
biosynthesis were identified in the
B. rapa
genome. In the present study, we investigated
GSL
content and expression levels of these
Br
MYB
TF
s in 38 accessions belonging to eight subspecies of
B. rapa
. Twelve identified
GSL
s were detected and were classified into three chemical groups based on patterns of
GSL
content and expression profiles of the
Br
MYB
TF
s.
GSL
content and
Br
MYB
TF
expression levels differed among genotypes, including
B. rapa
subspecies
pekinensis
,
chinensis
and
rapa
.
Br
MYB
28.3
,
Br
MYB
51.1
and
Br
MYB
122.2
positively regulated
GSL
content in 38 accessions. Furthermore, expression levels of
Br
MYB
28
s and
Br
MYB
34.3
increased under most abiotic and biotic stress treatments. The three
Br
MYB
51
paralogs also showed drastically increased expression levels after infection with
Pectobacterium carotovorum
. The results of the present study improve our understanding of the functional diversity of these 13
Br
MYB
TF
s during the evolution of polyploid
B. rapa
. |
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ISSN: | 2211-5463 2211-5463 |
DOI: | 10.1002/2211-5463.12231 |