Ameliorative effect of acetylshikonin on ovalbumin (OVA)‐induced allergic rhinitis in mice through the inhibition of Th2 cytokine production and mast cell histamine release
Acetylshikonin has long been known as an anti‐inflammatory and antioxidative reagent. However, the anti‐allergic effect has not been studied. The aim of this study was to evaluate the effect of acetylshikonin on allergic rhinitis (AR) in mice. Mice were sensitized by intraperitoneal injection of OVA...
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| Veröffentlicht in: | APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2019-10, Vol.127 (10), p.688-695 |
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| description | Acetylshikonin has long been known as an anti‐inflammatory and antioxidative reagent. However, the anti‐allergic effect has not been studied. The aim of this study was to evaluate the effect of acetylshikonin on allergic rhinitis (AR) in mice. Mice were sensitized by intraperitoneal injection of OVA and aluminum hydroxide and challenged with intranasal instillation of OVA. Acetylshikonin was administered orally after nasal cavities challenge. Severity of allergic rhinitis was assessed according to nasal symptoms; serum OVA‐specific immunoglobulin E (IgE), IgG1, and IgG2a level; and interleukin (IL)‐4, IL‐10, IL‐5, IL‐13, TNF‐α, IL‐12, and interferon (INF)‐γ levels in nasal lavage fluid (NALF). Additionally, the histological change and the release of histamine in serum and nasal lavage fluid were evaluated by acid‐Schiff stain and ELISA. Acetylshikonin attenuated manifestation of nasal symptoms in sensitized mice and inhibited production of Th2‐related OVA‐specific IgE, IgG1, and Th2 cell‐produced IL‐4, IL‐5, IL‐13, and mast cell produced histamine; however, it had no effect on Th1 cell‐produced cytokines, like INF‐γ. In addition, the degree of inflammatory cell infiltration and goblet cell hyperplasia was attenuated by acetylshikonin treatment. Our results suggest that acetylshikonin effectively reduces allergic inflammation in a mouse model of allergic rhinitis by its anti‐allergic and anti‐inflammatory properties. |
| doi_str_mv | 10.1111/apm.12984 |
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However, the anti‐allergic effect has not been studied. The aim of this study was to evaluate the effect of acetylshikonin on allergic rhinitis (AR) in mice. Mice were sensitized by intraperitoneal injection of OVA and aluminum hydroxide and challenged with intranasal instillation of OVA. Acetylshikonin was administered orally after nasal cavities challenge. Severity of allergic rhinitis was assessed according to nasal symptoms; serum OVA‐specific immunoglobulin E (IgE), IgG1, and IgG2a level; and interleukin (IL)‐4, IL‐10, IL‐5, IL‐13, TNF‐α, IL‐12, and interferon (INF)‐γ levels in nasal lavage fluid (NALF). Additionally, the histological change and the release of histamine in serum and nasal lavage fluid were evaluated by acid‐Schiff stain and ELISA. Acetylshikonin attenuated manifestation of nasal symptoms in sensitized mice and inhibited production of Th2‐related OVA‐specific IgE, IgG1, and Th2 cell‐produced IL‐4, IL‐5, IL‐13, and mast cell produced histamine; however, it had no effect on Th1 cell‐produced cytokines, like INF‐γ. In addition, the degree of inflammatory cell infiltration and goblet cell hyperplasia was attenuated by acetylshikonin treatment. Our results suggest that acetylshikonin effectively reduces allergic inflammation in a mouse model of allergic rhinitis by its anti‐allergic and anti‐inflammatory properties.</description><identifier>ISSN: 0903-4641</identifier><identifier>EISSN: 1600-0463</identifier><identifier>DOI: 10.1111/apm.12984</identifier><identifier>PMID: 31344274</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Acetylshikonin ; Administration, Oral ; Allergens - administration & dosage ; Allergic rhinitis ; Aluminum ; Aluminum hydroxide ; Animals ; Anthraquinones - administration & dosage ; cytokine ; Cytokines ; Cytokines - antagonists & inhibitors ; Disease Models, Animal ; Enzyme-linked immunosorbent assay ; Helper cells ; Histamine ; Histamine Release - drug effects ; Hyperplasia ; Hypersensitivity ; IL‐4 ; Immunoglobulin E ; Immunoglobulin G ; Immunologic Factors - administration & dosage ; Inflammation ; Injections, Intraperitoneal ; Interferon ; Interleukins ; Lymphocytes T ; Mast Cells - drug effects ; Mice ; Nose ; Oral administration ; Ovalbumin ; Ovalbumin - administration & dosage ; Reagents ; Rhinitis ; Rhinitis, Allergic - chemically induced ; Rhinitis, Allergic - drug therapy ; Rhinitis, Allergic - pathology ; Th2 Cells - drug effects ; Treatment Outcome ; Tumor necrosis factor</subject><ispartof>APMIS : acta pathologica, microbiologica et immunologica Scandinavica, 2019-10, Vol.127 (10), p.688-695</ispartof><rights>2019 APMIS. Published by John Wiley & Sons Ltd</rights><rights>2019 APMIS. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 APMIS Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3884-d88554fee9b41b5a9d9ac612a9f1d4ed13caeaa6e0444ed81a8291821f8eab173</citedby><cites>FETCH-LOGICAL-c3884-d88554fee9b41b5a9d9ac612a9f1d4ed13caeaa6e0444ed81a8291821f8eab173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fapm.12984$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fapm.12984$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31344274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fan, Xi‐Hui</creatorcontrib><creatorcontrib>Cheng, Lei</creatorcontrib><creatorcontrib>Yan, Ai‐Hui</creatorcontrib><title>Ameliorative effect of acetylshikonin on ovalbumin (OVA)‐induced allergic rhinitis in mice through the inhibition of Th2 cytokine production and mast cell histamine release</title><title>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</title><addtitle>APMIS</addtitle><description>Acetylshikonin has long been known as an anti‐inflammatory and antioxidative reagent. However, the anti‐allergic effect has not been studied. The aim of this study was to evaluate the effect of acetylshikonin on allergic rhinitis (AR) in mice. Mice were sensitized by intraperitoneal injection of OVA and aluminum hydroxide and challenged with intranasal instillation of OVA. Acetylshikonin was administered orally after nasal cavities challenge. Severity of allergic rhinitis was assessed according to nasal symptoms; serum OVA‐specific immunoglobulin E (IgE), IgG1, and IgG2a level; and interleukin (IL)‐4, IL‐10, IL‐5, IL‐13, TNF‐α, IL‐12, and interferon (INF)‐γ levels in nasal lavage fluid (NALF). Additionally, the histological change and the release of histamine in serum and nasal lavage fluid were evaluated by acid‐Schiff stain and ELISA. Acetylshikonin attenuated manifestation of nasal symptoms in sensitized mice and inhibited production of Th2‐related OVA‐specific IgE, IgG1, and Th2 cell‐produced IL‐4, IL‐5, IL‐13, and mast cell produced histamine; however, it had no effect on Th1 cell‐produced cytokines, like INF‐γ. In addition, the degree of inflammatory cell infiltration and goblet cell hyperplasia was attenuated by acetylshikonin treatment. Our results suggest that acetylshikonin effectively reduces allergic inflammation in a mouse model of allergic rhinitis by its anti‐allergic and anti‐inflammatory properties.</description><subject>Acetylshikonin</subject><subject>Administration, Oral</subject><subject>Allergens - administration & dosage</subject><subject>Allergic rhinitis</subject><subject>Aluminum</subject><subject>Aluminum hydroxide</subject><subject>Animals</subject><subject>Anthraquinones - administration & dosage</subject><subject>cytokine</subject><subject>Cytokines</subject><subject>Cytokines - antagonists & inhibitors</subject><subject>Disease Models, Animal</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Helper cells</subject><subject>Histamine</subject><subject>Histamine Release - drug effects</subject><subject>Hyperplasia</subject><subject>Hypersensitivity</subject><subject>IL‐4</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin G</subject><subject>Immunologic Factors - administration & dosage</subject><subject>Inflammation</subject><subject>Injections, Intraperitoneal</subject><subject>Interferon</subject><subject>Interleukins</subject><subject>Lymphocytes T</subject><subject>Mast Cells - drug effects</subject><subject>Mice</subject><subject>Nose</subject><subject>Oral administration</subject><subject>Ovalbumin</subject><subject>Ovalbumin - administration & dosage</subject><subject>Reagents</subject><subject>Rhinitis</subject><subject>Rhinitis, Allergic - chemically induced</subject><subject>Rhinitis, Allergic - drug therapy</subject><subject>Rhinitis, Allergic - pathology</subject><subject>Th2 Cells - drug effects</subject><subject>Treatment Outcome</subject><subject>Tumor necrosis factor</subject><issn>0903-4641</issn><issn>1600-0463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9u1DAQhy0EokvhwAsgS1zoIa3H8Wad46riT6VW7aFwjSbOuHHrJIvtFO2NR-BJeCieBG-3cKtlaTSeT99Y-jH2FsQx5HOCm-EYZK3VM7aASohCqKp8zhaiFmWhKgUH7FWMt0KA1NXqJTsooVRKrtSC_V4P5N0UMLl74mQtmcQny9FQ2vrYu7tpdCOf8r1H385Dbj5cflsf_fn5y43dbKjj6D2FG2d46N3okos8Q4MzxFMfpvmmz5XyW-_aPN2pLL_uJTfbNN25kfgmTNn0MMKx4wPGxA15z3sXEw47JJAnjPSavbDoI715rIfs66eP16dfivPLz2en6_PClFqrotN6uVSWqG4VtEusuxpNBRJrC52iDkqDhFiRUCq3GlDLGrQEqwlbWJWH7P3em7_2faaYmttpDmNe2UipV6UUoCFTR3vKhCnGQLbZBDdg2DYgml0yTU6meUgms-8ejXM7UPef_BdFBk72wA_nafu0qVlfXeyVfwG8-5yT</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Fan, Xi‐Hui</creator><creator>Cheng, Lei</creator><creator>Yan, Ai‐Hui</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>201910</creationdate><title>Ameliorative effect of acetylshikonin on ovalbumin (OVA)‐induced allergic rhinitis in mice through the inhibition of Th2 cytokine production and mast cell histamine release</title><author>Fan, Xi‐Hui ; Cheng, Lei ; Yan, Ai‐Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3884-d88554fee9b41b5a9d9ac612a9f1d4ed13caeaa6e0444ed81a8291821f8eab173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetylshikonin</topic><topic>Administration, Oral</topic><topic>Allergens - administration & dosage</topic><topic>Allergic rhinitis</topic><topic>Aluminum</topic><topic>Aluminum hydroxide</topic><topic>Animals</topic><topic>Anthraquinones - administration & dosage</topic><topic>cytokine</topic><topic>Cytokines</topic><topic>Cytokines - antagonists & inhibitors</topic><topic>Disease Models, Animal</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Helper cells</topic><topic>Histamine</topic><topic>Histamine Release - drug effects</topic><topic>Hyperplasia</topic><topic>Hypersensitivity</topic><topic>IL‐4</topic><topic>Immunoglobulin E</topic><topic>Immunoglobulin G</topic><topic>Immunologic Factors - administration & dosage</topic><topic>Inflammation</topic><topic>Injections, Intraperitoneal</topic><topic>Interferon</topic><topic>Interleukins</topic><topic>Lymphocytes T</topic><topic>Mast Cells - drug effects</topic><topic>Mice</topic><topic>Nose</topic><topic>Oral administration</topic><topic>Ovalbumin</topic><topic>Ovalbumin - administration & dosage</topic><topic>Reagents</topic><topic>Rhinitis</topic><topic>Rhinitis, Allergic - chemically induced</topic><topic>Rhinitis, Allergic - drug therapy</topic><topic>Rhinitis, Allergic - pathology</topic><topic>Th2 Cells - drug effects</topic><topic>Treatment Outcome</topic><topic>Tumor necrosis factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Xi‐Hui</creatorcontrib><creatorcontrib>Cheng, Lei</creatorcontrib><creatorcontrib>Yan, Ai‐Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, Xi‐Hui</au><au>Cheng, Lei</au><au>Yan, Ai‐Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ameliorative effect of acetylshikonin on ovalbumin (OVA)‐induced allergic rhinitis in mice through the inhibition of Th2 cytokine production and mast cell histamine release</atitle><jtitle>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</jtitle><addtitle>APMIS</addtitle><date>2019-10</date><risdate>2019</risdate><volume>127</volume><issue>10</issue><spage>688</spage><epage>695</epage><pages>688-695</pages><issn>0903-4641</issn><eissn>1600-0463</eissn><abstract>Acetylshikonin has long been known as an anti‐inflammatory and antioxidative reagent. However, the anti‐allergic effect has not been studied. The aim of this study was to evaluate the effect of acetylshikonin on allergic rhinitis (AR) in mice. Mice were sensitized by intraperitoneal injection of OVA and aluminum hydroxide and challenged with intranasal instillation of OVA. Acetylshikonin was administered orally after nasal cavities challenge. Severity of allergic rhinitis was assessed according to nasal symptoms; serum OVA‐specific immunoglobulin E (IgE), IgG1, and IgG2a level; and interleukin (IL)‐4, IL‐10, IL‐5, IL‐13, TNF‐α, IL‐12, and interferon (INF)‐γ levels in nasal lavage fluid (NALF). Additionally, the histological change and the release of histamine in serum and nasal lavage fluid were evaluated by acid‐Schiff stain and ELISA. Acetylshikonin attenuated manifestation of nasal symptoms in sensitized mice and inhibited production of Th2‐related OVA‐specific IgE, IgG1, and Th2 cell‐produced IL‐4, IL‐5, IL‐13, and mast cell produced histamine; however, it had no effect on Th1 cell‐produced cytokines, like INF‐γ. In addition, the degree of inflammatory cell infiltration and goblet cell hyperplasia was attenuated by acetylshikonin treatment. Our results suggest that acetylshikonin effectively reduces allergic inflammation in a mouse model of allergic rhinitis by its anti‐allergic and anti‐inflammatory properties.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31344274</pmid><doi>10.1111/apm.12984</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylshikonin Administration, Oral Allergens - administration & dosage Allergic rhinitis Aluminum Aluminum hydroxide Animals Anthraquinones - administration & dosage cytokine Cytokines Cytokines - antagonists & inhibitors Disease Models, Animal Enzyme-linked immunosorbent assay Helper cells Histamine Histamine Release - drug effects Hyperplasia Hypersensitivity IL‐4 Immunoglobulin E Immunoglobulin G Immunologic Factors - administration & dosage Inflammation Injections, Intraperitoneal Interferon Interleukins Lymphocytes T Mast Cells - drug effects Mice Nose Oral administration Ovalbumin Ovalbumin - administration & dosage Reagents Rhinitis Rhinitis, Allergic - chemically induced Rhinitis, Allergic - drug therapy Rhinitis, Allergic - pathology Th2 Cells - drug effects Treatment Outcome Tumor necrosis factor |
| title | Ameliorative effect of acetylshikonin on ovalbumin (OVA)‐induced allergic rhinitis in mice through the inhibition of Th2 cytokine production and mast cell histamine release |
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