Hempseed protein hydrolysates’ effects on the proliferation and induced oxidative stress in normal and cancer cell lines
Food proteins from different sources can provide beneficial effects on human health by releasing the bioactive peptides that are integral part of their native structure. In this study, we tested the biological potential of hempseed protein hydrolysates (HPHs) obtained from hempseed cake protein isol...
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| Veröffentlicht in: | Molecular biology reports 2019-12, Vol.46 (6), p.6079-6085 |
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| creator | Logarušić, Marijan Slivac, Igor Radošević, Kristina Bagović, Martina Redovniković, Ivana Radojčić Srček, Višnja Gaurina |
| description | Food proteins from different sources can provide beneficial effects on human health by releasing the bioactive peptides that are integral part of their native structure. In this study, we tested the biological potential of hempseed protein hydrolysates (HPHs) obtained from hempseed cake protein isolate. The HPHs were prepared by enzyme hydrolysis using three different proteases of microbial origin: Alcalase®, Neutrase® and Protamex®. The antioxidant activity of the obtained hydrolysates was determined by oxygen radical absorbance capacity (ORAC) assay, while the proliferative effects on normal (HaCaT) and cancer (HeLa) cells were determined by the CellTiter 96
®
AQ
ueous
One Solution Reagent (MTS) assay. HPHs showed dose-dependent antiproliferative effects on HeLa cells and stimulatory effects on the proliferation of HaCaT cells. HPH obtained by Neutrase
®
(HPH-N) showed the highest antioxidant activity expressed as an ORAC value. The protective effect of HPH-N on H
2
O
2
-induced oxidative stress in normal and cancer cells was evaluated and 1 mg/mL of HPH-N significantly reduced the formation of intracellular reactive oxygen species (ROS) in both cell lines. The obtained results indicate the benefits of HPHs as potential natural antioxidants for the food industry and contribute to the growing trend of utilizing hempseed by-products. |
| doi_str_mv | 10.1007/s11033-019-05043-8 |
| format | Article |
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®
AQ
ueous
One Solution Reagent (MTS) assay. HPHs showed dose-dependent antiproliferative effects on HeLa cells and stimulatory effects on the proliferation of HaCaT cells. HPH obtained by Neutrase
®
(HPH-N) showed the highest antioxidant activity expressed as an ORAC value. The protective effect of HPH-N on H
2
O
2
-induced oxidative stress in normal and cancer cells was evaluated and 1 mg/mL of HPH-N significantly reduced the formation of intracellular reactive oxygen species (ROS) in both cell lines. The obtained results indicate the benefits of HPHs as potential natural antioxidants for the food industry and contribute to the growing trend of utilizing hempseed by-products.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-019-05043-8</identifier><identifier>PMID: 31493283</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Antioxidants ; Antioxidants - metabolism ; Biomedical and Life Sciences ; cakes ; Cancer ; Cannabis - metabolism ; Cell Line ; Cell proliferation ; Cell Proliferation - drug effects ; dose response ; Endopeptidases - metabolism ; enzymatic hydrolysis ; Food industry ; Food sources ; HeLa Cells ; Histology ; human health ; Humans ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Hydrolysates ; Hydrolysis ; Life Sciences ; Morphology ; neoplasm cells ; Original Article ; Oxidative stress ; Oxidative Stress - drug effects ; oxygen radical absorbance capacity ; Peptide Hydrolases ; peptides ; Peptides - metabolism ; Plant Extracts - metabolism ; Plant Extracts - pharmacology ; protective effect ; Protein Hydrolysates - metabolism ; Protein Hydrolysates - pharmacology ; protein isolates ; proteinases ; Proteins ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Subtilisin ; Tumor cell lines</subject><ispartof>Molecular biology reports, 2019-12, Vol.46 (6), p.6079-6085</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Molecular Biology Reports is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-e8230a8246178cbe1ee82bc4cf79c3e1a7ed9214416d8953a3ff1354c1d43ef93</citedby><cites>FETCH-LOGICAL-c408t-e8230a8246178cbe1ee82bc4cf79c3e1a7ed9214416d8953a3ff1354c1d43ef93</cites><orcidid>0000-0001-6591-3810</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-019-05043-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-019-05043-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31493283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Logarušić, Marijan</creatorcontrib><creatorcontrib>Slivac, Igor</creatorcontrib><creatorcontrib>Radošević, Kristina</creatorcontrib><creatorcontrib>Bagović, Martina</creatorcontrib><creatorcontrib>Redovniković, Ivana Radojčić</creatorcontrib><creatorcontrib>Srček, Višnja Gaurina</creatorcontrib><title>Hempseed protein hydrolysates’ effects on the proliferation and induced oxidative stress in normal and cancer cell lines</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Food proteins from different sources can provide beneficial effects on human health by releasing the bioactive peptides that are integral part of their native structure. In this study, we tested the biological potential of hempseed protein hydrolysates (HPHs) obtained from hempseed cake protein isolate. The HPHs were prepared by enzyme hydrolysis using three different proteases of microbial origin: Alcalase®, Neutrase® and Protamex®. The antioxidant activity of the obtained hydrolysates was determined by oxygen radical absorbance capacity (ORAC) assay, while the proliferative effects on normal (HaCaT) and cancer (HeLa) cells were determined by the CellTiter 96
®
AQ
ueous
One Solution Reagent (MTS) assay. HPHs showed dose-dependent antiproliferative effects on HeLa cells and stimulatory effects on the proliferation of HaCaT cells. HPH obtained by Neutrase
®
(HPH-N) showed the highest antioxidant activity expressed as an ORAC value. The protective effect of HPH-N on H
2
O
2
-induced oxidative stress in normal and cancer cells was evaluated and 1 mg/mL of HPH-N significantly reduced the formation of intracellular reactive oxygen species (ROS) in both cell lines. The obtained results indicate the benefits of HPHs as potential natural antioxidants for the food industry and contribute to the growing trend of utilizing hempseed by-products.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>cakes</subject><subject>Cancer</subject><subject>Cannabis - metabolism</subject><subject>Cell Line</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>dose response</subject><subject>Endopeptidases - metabolism</subject><subject>enzymatic hydrolysis</subject><subject>Food industry</subject><subject>Food sources</subject><subject>HeLa Cells</subject><subject>Histology</subject><subject>human health</subject><subject>Humans</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Hydrolysates</subject><subject>Hydrolysis</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>neoplasm cells</subject><subject>Original Article</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>oxygen radical absorbance capacity</subject><subject>Peptide Hydrolases</subject><subject>peptides</subject><subject>Peptides - metabolism</subject><subject>Plant Extracts - metabolism</subject><subject>Plant Extracts - pharmacology</subject><subject>protective effect</subject><subject>Protein Hydrolysates - metabolism</subject><subject>Protein Hydrolysates - pharmacology</subject><subject>protein isolates</subject><subject>proteinases</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Subtilisin</subject><subject>Tumor cell lines</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1OHDEQha2IKAw_F8gissQmmwZX2552LyNEAhJSNmRteexyaNTtnri6UYYV18j1OEk8DAlSFllZevXV81M9xt6DOAUhmjMCEFJWAtpKaKFkZd6wBehGVqptzB5bCCmgUkbDPjsguhNCKGj0O7YvQbWyNnLBHi5xWBNi4Os8TtglfrsJeew35Cakp8dfHGNEPxEfE59ucYv1XcTspq4oLgXepTD7YjD-7EJR75HTlJGoDHga8-D6Z8y75DFzj33P-y4hHbG30fWExy_vIfv2-eLm_LK6_vrl6vzTdeWVMFOFppbCmVotoTF-hYBFWXnlY9N6ieAaDG0NSsEymFZLJ2MEqZWHoCTGVh6yjzvfEv3HjDTZoaNtDJdwnMnWWkNbDmO26Mk_6N0451TS2bo2WoFsllCoekf5PBJljHadu8HljQVht83YXTO2NGOfm7GmLH14sZ5XA4a_K3-qKIDcAVRG6Tvm17__Y_sb_ZGbWQ</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Logarušić, Marijan</creator><creator>Slivac, Igor</creator><creator>Radošević, Kristina</creator><creator>Bagović, Martina</creator><creator>Redovniković, Ivana Radojčić</creator><creator>Srček, Višnja Gaurina</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-6591-3810</orcidid></search><sort><creationdate>20191201</creationdate><title>Hempseed protein hydrolysates’ effects on the proliferation and induced oxidative stress in normal and cancer cell lines</title><author>Logarušić, Marijan ; Slivac, Igor ; Radošević, Kristina ; Bagović, Martina ; Redovniković, Ivana Radojčić ; Srček, Višnja Gaurina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-e8230a8246178cbe1ee82bc4cf79c3e1a7ed9214416d8953a3ff1354c1d43ef93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Antioxidants</topic><topic>Antioxidants - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>cakes</topic><topic>Cancer</topic><topic>Cannabis - metabolism</topic><topic>Cell Line</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>dose response</topic><topic>Endopeptidases - metabolism</topic><topic>enzymatic hydrolysis</topic><topic>Food industry</topic><topic>Food sources</topic><topic>HeLa Cells</topic><topic>Histology</topic><topic>human health</topic><topic>Humans</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Hydrolysates</topic><topic>Hydrolysis</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>neoplasm cells</topic><topic>Original Article</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>oxygen radical absorbance capacity</topic><topic>Peptide Hydrolases</topic><topic>peptides</topic><topic>Peptides - metabolism</topic><topic>Plant Extracts - metabolism</topic><topic>Plant Extracts - pharmacology</topic><topic>protective effect</topic><topic>Protein Hydrolysates - metabolism</topic><topic>Protein Hydrolysates - pharmacology</topic><topic>protein isolates</topic><topic>proteinases</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Subtilisin</topic><topic>Tumor cell lines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Logarušić, Marijan</creatorcontrib><creatorcontrib>Slivac, Igor</creatorcontrib><creatorcontrib>Radošević, Kristina</creatorcontrib><creatorcontrib>Bagović, Martina</creatorcontrib><creatorcontrib>Redovniković, Ivana Radojčić</creatorcontrib><creatorcontrib>Srček, Višnja Gaurina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Logarušić, Marijan</au><au>Slivac, Igor</au><au>Radošević, Kristina</au><au>Bagović, Martina</au><au>Redovniković, Ivana Radojčić</au><au>Srček, Višnja Gaurina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hempseed protein hydrolysates’ effects on the proliferation and induced oxidative stress in normal and cancer cell lines</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>46</volume><issue>6</issue><spage>6079</spage><epage>6085</epage><pages>6079-6085</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Food proteins from different sources can provide beneficial effects on human health by releasing the bioactive peptides that are integral part of their native structure. In this study, we tested the biological potential of hempseed protein hydrolysates (HPHs) obtained from hempseed cake protein isolate. The HPHs were prepared by enzyme hydrolysis using three different proteases of microbial origin: Alcalase®, Neutrase® and Protamex®. The antioxidant activity of the obtained hydrolysates was determined by oxygen radical absorbance capacity (ORAC) assay, while the proliferative effects on normal (HaCaT) and cancer (HeLa) cells were determined by the CellTiter 96
®
AQ
ueous
One Solution Reagent (MTS) assay. HPHs showed dose-dependent antiproliferative effects on HeLa cells and stimulatory effects on the proliferation of HaCaT cells. HPH obtained by Neutrase
®
(HPH-N) showed the highest antioxidant activity expressed as an ORAC value. The protective effect of HPH-N on H
2
O
2
-induced oxidative stress in normal and cancer cells was evaluated and 1 mg/mL of HPH-N significantly reduced the formation of intracellular reactive oxygen species (ROS) in both cell lines. The obtained results indicate the benefits of HPHs as potential natural antioxidants for the food industry and contribute to the growing trend of utilizing hempseed by-products.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31493283</pmid><doi>10.1007/s11033-019-05043-8</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-6591-3810</orcidid></addata></record> |
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| issn | 0301-4851 1573-4978 |
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| subjects | Animal Anatomy Animal Biochemistry Antioxidants Antioxidants - metabolism Biomedical and Life Sciences cakes Cancer Cannabis - metabolism Cell Line Cell proliferation Cell Proliferation - drug effects dose response Endopeptidases - metabolism enzymatic hydrolysis Food industry Food sources HeLa Cells Histology human health Humans Hydrogen peroxide Hydrogen Peroxide - pharmacology Hydrolysates Hydrolysis Life Sciences Morphology neoplasm cells Original Article Oxidative stress Oxidative Stress - drug effects oxygen radical absorbance capacity Peptide Hydrolases peptides Peptides - metabolism Plant Extracts - metabolism Plant Extracts - pharmacology protective effect Protein Hydrolysates - metabolism Protein Hydrolysates - pharmacology protein isolates proteinases Proteins Reactive oxygen species Reactive Oxygen Species - metabolism Subtilisin Tumor cell lines |
| title | Hempseed protein hydrolysates’ effects on the proliferation and induced oxidative stress in normal and cancer cell lines |
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