Application of the slope‐ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats
The slope‐ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp‐supplemented diets to establish the Trp concentration range over which th...
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| Veröffentlicht in: | Animal science journal 2019-09, Vol.90 (9), p.1270-1277 |
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| creator | Sato, Hiroyuki Miura, Makoto Fujieda, Takeshi Toyomizu, Masaaki |
| description | The slope‐ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp‐supplemented diets to establish the Trp concentration range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp‐supplemented diets from three different sources: l‐Trp alone, SBM, or l‐Trp mixed with other AAs to reflect AA levels in the test SBM (AA‐mix). Weight gain in rats increased linearly with supplemental Trp intake (p |
| doi_str_mv | 10.1111/asj.13279 |
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In a preliminary study, rats were fed graded levels of Trp‐supplemented diets to establish the Trp concentration range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp‐supplemented diets from three different sources: l‐Trp alone, SBM, or l‐Trp mixed with other AAs to reflect AA levels in the test SBM (AA‐mix). Weight gain in rats increased linearly with supplemental Trp intake (p < .05) for all Trp sources. Compared to the slope achieved with l‐Trp alone, the estimated availability of Trp in SBM was 84.4%, while for the AA‐mix it was 93.4%. It is evident that the 6.6% reduction in l‐Trp availability in AA‐mix is due to metabolic costs derived from excess levels of other AAs beside Trp, given that the absorption of crystalline l‐Trp in the small intestine is 100%. In conclusion, the Trp availability of SBM was estimated to be around 90.4% (i.e., 84.4/93.4 × 100) after correcting for the effects of the other AAs in SBM.</description><identifier>ISSN: 1344-3941</identifier><identifier>EISSN: 1740-0929</identifier><identifier>DOI: 10.1111/asj.13279</identifier><identifier>PMID: 31381237</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Amino acids ; Animal Feed ; Animals ; Availability ; Biological Availability ; Body weight gain ; Diet ; Dietary Supplements ; Glycine max - chemistry ; Male ; Rats ; Rodents ; slope ratio assay ; Small intestine ; Soy Foods - analysis ; soybean meal ; Soybeans ; Tryptophan ; Weight Gain</subject><ispartof>Animal science journal, 2019-09, Vol.90 (9), p.1270-1277</ispartof><rights>2019 Japanese Society of Animal Science</rights><rights>2019 Japanese Society of Animal Science.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3779-14a64fe4538ced8359dfc283b4f1c3a449153ddfd1fdf1a8125f193878e1b4963</citedby><cites>FETCH-LOGICAL-c3779-14a64fe4538ced8359dfc283b4f1c3a449153ddfd1fdf1a8125f193878e1b4963</cites><orcidid>0000-0001-7339-8281</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fasj.13279$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fasj.13279$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31381237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sato, Hiroyuki</creatorcontrib><creatorcontrib>Miura, Makoto</creatorcontrib><creatorcontrib>Fujieda, Takeshi</creatorcontrib><creatorcontrib>Toyomizu, Masaaki</creatorcontrib><title>Application of the slope‐ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats</title><title>Animal science journal</title><addtitle>Anim Sci J</addtitle><description>The slope‐ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp‐supplemented diets to establish the Trp concentration range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp‐supplemented diets from three different sources: l‐Trp alone, SBM, or l‐Trp mixed with other AAs to reflect AA levels in the test SBM (AA‐mix). Weight gain in rats increased linearly with supplemental Trp intake (p < .05) for all Trp sources. Compared to the slope achieved with l‐Trp alone, the estimated availability of Trp in SBM was 84.4%, while for the AA‐mix it was 93.4%. It is evident that the 6.6% reduction in l‐Trp availability in AA‐mix is due to metabolic costs derived from excess levels of other AAs beside Trp, given that the absorption of crystalline l‐Trp in the small intestine is 100%. In conclusion, the Trp availability of SBM was estimated to be around 90.4% (i.e., 84.4/93.4 × 100) after correcting for the effects of the other AAs in SBM.</description><subject>Amino acids</subject><subject>Animal Feed</subject><subject>Animals</subject><subject>Availability</subject><subject>Biological Availability</subject><subject>Body weight gain</subject><subject>Diet</subject><subject>Dietary Supplements</subject><subject>Glycine max - chemistry</subject><subject>Male</subject><subject>Rats</subject><subject>Rodents</subject><subject>slope ratio assay</subject><subject>Small intestine</subject><subject>Soy Foods - analysis</subject><subject>soybean meal</subject><subject>Soybeans</subject><subject>Tryptophan</subject><subject>Weight Gain</subject><issn>1344-3941</issn><issn>1740-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL9OwzAQhy0EoqUw8ALIEhND2lzsNPFYVfxVJQZgjpzEblylcbBdqgxIPALPyJPgksKGF59On7_z_RA6h3AM_ky4XY2BRAk7QENIaBiELGKHviaUBoRRGKATa1dhCAkL42M0IEBSiEgyRO-ztq1VwZ3SDdYSu0pgW-tWfH18ml0XL43eugpza3mHnSiqRr1uBHYaC-vUmjtfm651uq14g_kbVzXPVa1ch1WDre5y4ftrwWssRbl71-lNs8Tebk_RkeS1FWf7e4Rebq6f53fB4vH2fj5bBAVJEhYA5VMqBY1JWogyJTErZRGlJKcSCsIpZRCTspQlyFIC96vFEhhJk1RATtmUjNBl722N9p-3LlvpjWn8yCzynoiGQMFTVz1VGG2tETJrjV_QdBmE2S7ozAed_QTt2Yu9cZOvRflH_ibrgUkPbFUtuv9N2ezpoVd-A95Yimc</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Sato, Hiroyuki</creator><creator>Miura, Makoto</creator><creator>Fujieda, Takeshi</creator><creator>Toyomizu, Masaaki</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0001-7339-8281</orcidid></search><sort><creationdate>201909</creationdate><title>Application of the slope‐ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats</title><author>Sato, Hiroyuki ; Miura, Makoto ; Fujieda, Takeshi ; Toyomizu, Masaaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3779-14a64fe4538ced8359dfc283b4f1c3a449153ddfd1fdf1a8125f193878e1b4963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Amino acids</topic><topic>Animal Feed</topic><topic>Animals</topic><topic>Availability</topic><topic>Biological Availability</topic><topic>Body weight gain</topic><topic>Diet</topic><topic>Dietary Supplements</topic><topic>Glycine max - chemistry</topic><topic>Male</topic><topic>Rats</topic><topic>Rodents</topic><topic>slope ratio assay</topic><topic>Small intestine</topic><topic>Soy Foods - analysis</topic><topic>soybean meal</topic><topic>Soybeans</topic><topic>Tryptophan</topic><topic>Weight Gain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sato, Hiroyuki</creatorcontrib><creatorcontrib>Miura, Makoto</creatorcontrib><creatorcontrib>Fujieda, Takeshi</creatorcontrib><creatorcontrib>Toyomizu, Masaaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Animal science journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sato, Hiroyuki</au><au>Miura, Makoto</au><au>Fujieda, Takeshi</au><au>Toyomizu, Masaaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of the slope‐ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats</atitle><jtitle>Animal science journal</jtitle><addtitle>Anim Sci J</addtitle><date>2019-09</date><risdate>2019</risdate><volume>90</volume><issue>9</issue><spage>1270</spage><epage>1277</epage><pages>1270-1277</pages><issn>1344-3941</issn><eissn>1740-0929</eissn><abstract>The slope‐ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp‐supplemented diets to establish the Trp concentration range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp‐supplemented diets from three different sources: l‐Trp alone, SBM, or l‐Trp mixed with other AAs to reflect AA levels in the test SBM (AA‐mix). Weight gain in rats increased linearly with supplemental Trp intake (p < .05) for all Trp sources. Compared to the slope achieved with l‐Trp alone, the estimated availability of Trp in SBM was 84.4%, while for the AA‐mix it was 93.4%. It is evident that the 6.6% reduction in l‐Trp availability in AA‐mix is due to metabolic costs derived from excess levels of other AAs beside Trp, given that the absorption of crystalline l‐Trp in the small intestine is 100%. 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| subjects | Amino acids Animal Feed Animals Availability Biological Availability Body weight gain Diet Dietary Supplements Glycine max - chemistry Male Rats Rodents slope ratio assay Small intestine Soy Foods - analysis soybean meal Soybeans Tryptophan Weight Gain |
| title | Application of the slope‐ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats |
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