Nicotiana benthamiana exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans
The oomycete Phytophthora infestans is the causal agent of potato late blight, one of the most devastating pathogens for potato. To investigate the plant mechanisms for resistance against P. infestans , the Solanaceae model plant Nicotiana benthamiana was employed in this study. Previously, we repor...
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| Veröffentlicht in: | Journal of general plant pathology : JGPP 2019-09, Vol.85 (5), p.347-355 |
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| creator | Mizuno, Yuri Imano, Sayaka Camagna, Maurizio Suzuki, Takamasa Tanaka, Aiko Sato, Ikuo Chiba, Sotaro Kawakita, Kazuhito Takemoto, Daigo |
| description | The oomycete
Phytophthora infestans
is the causal agent of potato late blight, one of the most devastating pathogens for potato. To investigate the plant mechanisms for resistance against
P. infestans
, the Solanaceae model plant
Nicotiana benthamiana
was employed in this study. Previously, we reported that NbNup75, a nuclear pore complex protein, is required for the resistance against
P. infestans
, suggesting that nuclear-pore-mediated transport is involved in the induction of defense responses. In this study, we investigated the role of
N. benthamiana
exportin 1
NbXpo1
in disease resistance. Mammalian and yeast exportin 1 proteins are known as a regulator for nuclear-pore-mediated export of proteins and RNAs.
NbXpo1
-silenced
N. benthamiana
showed minor growth defects and significantly decreased resistance to
P. infestans
. Gene silencing of
NbXpo1
compromised defense responses induced by the elicitor INF1 (a secretory protein of
P. infestans
), such as production of the phytoalexin capsidiol and induction of cell death. In
NbXpo1
-silenced plants, the genes for capsidiol biosynthesis,
NbEAS
, and defense-related MAP kinase,
NbWIPK
, were significantly downregulated, while genes encoding the plant defensin,
NbPDF
, and anti-microbial protein thionin,
NbTHI
, were upregulated. These results indicate that
N. benthamiana
Xpo1 is involved in activating a specific group of defense-related genes. |
| doi_str_mv | 10.1007/s10327-019-00855-9 |
| format | Article |
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Phytophthora infestans
is the causal agent of potato late blight, one of the most devastating pathogens for potato. To investigate the plant mechanisms for resistance against
P. infestans
, the Solanaceae model plant
Nicotiana benthamiana
was employed in this study. Previously, we reported that NbNup75, a nuclear pore complex protein, is required for the resistance against
P. infestans
, suggesting that nuclear-pore-mediated transport is involved in the induction of defense responses. In this study, we investigated the role of
N. benthamiana
exportin 1
NbXpo1
in disease resistance. Mammalian and yeast exportin 1 proteins are known as a regulator for nuclear-pore-mediated export of proteins and RNAs.
NbXpo1
-silenced
N. benthamiana
showed minor growth defects and significantly decreased resistance to
P. infestans
. Gene silencing of
NbXpo1
compromised defense responses induced by the elicitor INF1 (a secretory protein of
P. infestans
), such as production of the phytoalexin capsidiol and induction of cell death. In
NbXpo1
-silenced plants, the genes for capsidiol biosynthesis,
NbEAS
, and defense-related MAP kinase,
NbWIPK
, were significantly downregulated, while genes encoding the plant defensin,
NbPDF
, and anti-microbial protein thionin,
NbTHI
, were upregulated. These results indicate that
N. benthamiana
Xpo1 is involved in activating a specific group of defense-related genes.</description><identifier>ISSN: 1345-2630</identifier><identifier>EISSN: 1610-739X</identifier><identifier>DOI: 10.1007/s10327-019-00855-9</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Agriculture ; Antiinfectives and antibacterials ; Apoptosis ; Biomedical and Life Sciences ; Biosynthesis ; Blight ; Cell death ; Disease resistance ; Gene silencing ; Genes ; Host Responses ; Kinases ; Late blight ; Life Sciences ; MAP kinase ; Microbiology ; Microorganisms ; Nicotiana benthamiana ; Pathogens ; Phytophthora infestans ; Plant Pathology ; Plants (botany) ; Potatoes ; Proteins ; Yeast ; Yeasts</subject><ispartof>Journal of general plant pathology : JGPP, 2019-09, Vol.85 (5), p.347-355</ispartof><rights>The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019</rights><rights>Journal of General Plant Pathology is a copyright of Springer, (2019). All Rights Reserved.</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-2ddfefce4c29a0419ab300107e3bbd2896d738f5af351572a495675d22340b9d3</citedby><cites>FETCH-LOGICAL-c457t-2ddfefce4c29a0419ab300107e3bbd2896d738f5af351572a495675d22340b9d3</cites><orcidid>0000-0002-9816-7882</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10327-019-00855-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10327-019-00855-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Mizuno, Yuri</creatorcontrib><creatorcontrib>Imano, Sayaka</creatorcontrib><creatorcontrib>Camagna, Maurizio</creatorcontrib><creatorcontrib>Suzuki, Takamasa</creatorcontrib><creatorcontrib>Tanaka, Aiko</creatorcontrib><creatorcontrib>Sato, Ikuo</creatorcontrib><creatorcontrib>Chiba, Sotaro</creatorcontrib><creatorcontrib>Kawakita, Kazuhito</creatorcontrib><creatorcontrib>Takemoto, Daigo</creatorcontrib><title>Nicotiana benthamiana exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans</title><title>Journal of general plant pathology : JGPP</title><addtitle>J Gen Plant Pathol</addtitle><description>The oomycete
Phytophthora infestans
is the causal agent of potato late blight, one of the most devastating pathogens for potato. To investigate the plant mechanisms for resistance against
P. infestans
, the Solanaceae model plant
Nicotiana benthamiana
was employed in this study. Previously, we reported that NbNup75, a nuclear pore complex protein, is required for the resistance against
P. infestans
, suggesting that nuclear-pore-mediated transport is involved in the induction of defense responses. In this study, we investigated the role of
N. benthamiana
exportin 1
NbXpo1
in disease resistance. Mammalian and yeast exportin 1 proteins are known as a regulator for nuclear-pore-mediated export of proteins and RNAs.
NbXpo1
-silenced
N. benthamiana
showed minor growth defects and significantly decreased resistance to
P. infestans
. Gene silencing of
NbXpo1
compromised defense responses induced by the elicitor INF1 (a secretory protein of
P. infestans
), such as production of the phytoalexin capsidiol and induction of cell death. In
NbXpo1
-silenced plants, the genes for capsidiol biosynthesis,
NbEAS
, and defense-related MAP kinase,
NbWIPK
, were significantly downregulated, while genes encoding the plant defensin,
NbPDF
, and anti-microbial protein thionin,
NbTHI
, were upregulated. These results indicate that
N. benthamiana
Xpo1 is involved in activating a specific group of defense-related genes.</description><subject>Agriculture</subject><subject>Antiinfectives and antibacterials</subject><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Blight</subject><subject>Cell death</subject><subject>Disease resistance</subject><subject>Gene silencing</subject><subject>Genes</subject><subject>Host Responses</subject><subject>Kinases</subject><subject>Late blight</subject><subject>Life Sciences</subject><subject>MAP kinase</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Nicotiana benthamiana</subject><subject>Pathogens</subject><subject>Phytophthora infestans</subject><subject>Plant Pathology</subject><subject>Plants (botany)</subject><subject>Potatoes</subject><subject>Proteins</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>1345-2630</issn><issn>1610-739X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kU9rHSEUxYfSQtOkX6ArodvaXnUcn8sS-g9C20UD2ckddd4YJjpRHyQfLd-uvjeF7rLyevidc7mcrnvH4CMDUJ8KA8EVBaYpwE5Kql90Z2xgQJXQNy_bLHpJ-SDgdfemlFsAzoSSZ93Tz2BTDRiRjD7WGe9Os39YU64hEkZCIdnfH0L2jkwpE78EG2rKNER3sE1c58eacPEPDV9zamINKX4g1i8LcR7rTE7opmJ0La-EUjFaT3CPIZZK1lSxJrJg9WRcwn5uUnOmvY_k93HBOrdfxhY1-aO3XHSvJlyKf_vvPe-uv375c_mdXv369uPy8xW1vVSVcucmP1nfW64ReqZxFAAMlBfj6PhOD06J3SRxEpJJxbHXclDScS56GLUT5937Lbfddn9ou81tOuTYVhrOdzBwIQGep5jsmVJcNIpvlM2plOwns-Zwh_nRMDDHIs1WpGlFmlORRjeT2EylwXHv8__oZ1x_AZMJpRw</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Mizuno, Yuri</creator><creator>Imano, Sayaka</creator><creator>Camagna, Maurizio</creator><creator>Suzuki, Takamasa</creator><creator>Tanaka, Aiko</creator><creator>Sato, Ikuo</creator><creator>Chiba, Sotaro</creator><creator>Kawakita, Kazuhito</creator><creator>Takemoto, Daigo</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-9816-7882</orcidid></search><sort><creationdate>20190901</creationdate><title>Nicotiana benthamiana exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans</title><author>Mizuno, Yuri ; Imano, Sayaka ; Camagna, Maurizio ; Suzuki, Takamasa ; Tanaka, Aiko ; Sato, Ikuo ; Chiba, Sotaro ; Kawakita, Kazuhito ; Takemoto, Daigo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-2ddfefce4c29a0419ab300107e3bbd2896d738f5af351572a495675d22340b9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agriculture</topic><topic>Antiinfectives and antibacterials</topic><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Blight</topic><topic>Cell death</topic><topic>Disease resistance</topic><topic>Gene silencing</topic><topic>Genes</topic><topic>Host Responses</topic><topic>Kinases</topic><topic>Late blight</topic><topic>Life Sciences</topic><topic>MAP kinase</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Nicotiana benthamiana</topic><topic>Pathogens</topic><topic>Phytophthora infestans</topic><topic>Plant Pathology</topic><topic>Plants (botany)</topic><topic>Potatoes</topic><topic>Proteins</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mizuno, Yuri</creatorcontrib><creatorcontrib>Imano, Sayaka</creatorcontrib><creatorcontrib>Camagna, Maurizio</creatorcontrib><creatorcontrib>Suzuki, Takamasa</creatorcontrib><creatorcontrib>Tanaka, Aiko</creatorcontrib><creatorcontrib>Sato, Ikuo</creatorcontrib><creatorcontrib>Chiba, Sotaro</creatorcontrib><creatorcontrib>Kawakita, Kazuhito</creatorcontrib><creatorcontrib>Takemoto, Daigo</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of general plant pathology : JGPP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mizuno, Yuri</au><au>Imano, Sayaka</au><au>Camagna, Maurizio</au><au>Suzuki, Takamasa</au><au>Tanaka, Aiko</au><au>Sato, Ikuo</au><au>Chiba, Sotaro</au><au>Kawakita, Kazuhito</au><au>Takemoto, Daigo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nicotiana benthamiana exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans</atitle><jtitle>Journal of general plant pathology : JGPP</jtitle><stitle>J Gen Plant Pathol</stitle><date>2019-09-01</date><risdate>2019</risdate><volume>85</volume><issue>5</issue><spage>347</spage><epage>355</epage><pages>347-355</pages><issn>1345-2630</issn><eissn>1610-739X</eissn><abstract>The oomycete
Phytophthora infestans
is the causal agent of potato late blight, one of the most devastating pathogens for potato. To investigate the plant mechanisms for resistance against
P. infestans
, the Solanaceae model plant
Nicotiana benthamiana
was employed in this study. Previously, we reported that NbNup75, a nuclear pore complex protein, is required for the resistance against
P. infestans
, suggesting that nuclear-pore-mediated transport is involved in the induction of defense responses. In this study, we investigated the role of
N. benthamiana
exportin 1
NbXpo1
in disease resistance. Mammalian and yeast exportin 1 proteins are known as a regulator for nuclear-pore-mediated export of proteins and RNAs.
NbXpo1
-silenced
N. benthamiana
showed minor growth defects and significantly decreased resistance to
P. infestans
. Gene silencing of
NbXpo1
compromised defense responses induced by the elicitor INF1 (a secretory protein of
P. infestans
), such as production of the phytoalexin capsidiol and induction of cell death. In
NbXpo1
-silenced plants, the genes for capsidiol biosynthesis,
NbEAS
, and defense-related MAP kinase,
NbWIPK
, were significantly downregulated, while genes encoding the plant defensin,
NbPDF
, and anti-microbial protein thionin,
NbTHI
, were upregulated. These results indicate that
N. benthamiana
Xpo1 is involved in activating a specific group of defense-related genes.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><doi>10.1007/s10327-019-00855-9</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9816-7882</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 1345-2630 1610-739X |
| language | eng |
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| source | SpringerNature Journals |
| subjects | Agriculture Antiinfectives and antibacterials Apoptosis Biomedical and Life Sciences Biosynthesis Blight Cell death Disease resistance Gene silencing Genes Host Responses Kinases Late blight Life Sciences MAP kinase Microbiology Microorganisms Nicotiana benthamiana Pathogens Phytophthora infestans Plant Pathology Plants (botany) Potatoes Proteins Yeast Yeasts |
| title | Nicotiana benthamiana exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans |
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