Antigenaemia and antibody response to Leishmania donovani stage-specific antigens and rk39 antigen in human immunodeficiency virus-infected patients
In order to define the possible markers for the early diagnosis of asymptomatic visceral leishmaniasis in human immunodeficiency virus (HIV)-infected individuals, the antigenaemia and antibody response to stage-specific Leishmania donovani and rk39 antigens is assessed by enzyme-linked immunosorbent...
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| Veröffentlicht in: | British journal of biomedical science 2003-01, Vol.60 (4), p.210-216 |
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| creator | Malla, N. Sengupta, C. Dubey, M.L. Sud, A. Ansari, N.A. Salotra, P. |
| description | In order to define the possible markers for the early diagnosis of asymptomatic visceral leishmaniasis in human immunodeficiency virus (HIV)-infected individuals, the antigenaemia and antibody response to stage-specific Leishmania donovani and rk39 antigens is assessed by enzyme-linked immunosorbent assay (ELISA) and immunoreactivity to stage-specific antigens analysed by Western blot. Serum samples from two out of 100 HIV-infected individuals were found positive for antigenaemia, antibody response to stage-specific L. donovani antigens and rk39 antigen, and one sample was also positive for antigenaemia and antibody response to L. donovani antigens, while antibody detection to rk39 antigen was not carried on this sample. Additionally, one sample was found positive for amastigote antigenaemia and antibody response to amastigote antigen, while in this patient promastigote antigenaemia and antibody response to promastigote L. donovani and rk39 antigen could not be detected. One sample was found positive for antigenaemia, antibody response to amastigote antigen and negative for antibody response to promastigote antigen, while in this patient response to rk39 antigen was borderline. Although antibody response to rk39 antigen could be detected in 9/88 (10%) HIV-infected individuals, in six of these nine patients neither antigenaemia nor antibody response to stage-specific L. donovani antigens could be detected. All 10 confirmed visceral leishmaniasis and HIV-negative control patients had positive antigenaemia and antibody response to L. donovani amastigote and promastigote antigens, while all the normal healthy individuals were negative. The study indicated that detection of antibody response to rk39 antigen, amastigote antigenaemia and antibody response to amastigote antigen may prove to be better markers than detection of promastigote antigenaemia, antibody response to promastigote antigen and immunoblot reactivity. |
| doi_str_mv | 10.1080/09674845.2003.11783701 |
| format | Article |
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Serum samples from two out of 100 HIV-infected individuals were found positive for antigenaemia, antibody response to stage-specific L. donovani antigens and rk39 antigen, and one sample was also positive for antigenaemia and antibody response to L. donovani antigens, while antibody detection to rk39 antigen was not carried on this sample. Additionally, one sample was found positive for amastigote antigenaemia and antibody response to amastigote antigen, while in this patient promastigote antigenaemia and antibody response to promastigote L. donovani and rk39 antigen could not be detected. One sample was found positive for antigenaemia, antibody response to amastigote antigen and negative for antibody response to promastigote antigen, while in this patient response to rk39 antigen was borderline. Although antibody response to rk39 antigen could be detected in 9/88 (10%) HIV-infected individuals, in six of these nine patients neither antigenaemia nor antibody response to stage-specific L. donovani antigens could be detected. All 10 confirmed visceral leishmaniasis and HIV-negative control patients had positive antigenaemia and antibody response to L. donovani amastigote and promastigote antigens, while all the normal healthy individuals were negative. The study indicated that detection of antibody response to rk39 antigen, amastigote antigenaemia and antibody response to amastigote antigen may prove to be better markers than detection of promastigote antigenaemia, antibody response to promastigote antigen and immunoblot reactivity.</description><identifier>ISSN: 0967-4845</identifier><identifier>EISSN: 2474-0896</identifier><identifier>DOI: 10.1080/09674845.2003.11783701</identifier><identifier>PMID: 14725337</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Adolescent ; Adult ; AIDS-Related Opportunistic Infections - diagnosis ; Animals ; Antibodies, Protozoan - biosynthesis ; Antigens, Protozoan - blood ; Female ; HIV-1 ; Human immunodeficiency virus ; Humans ; Leishmania donovani ; Leishmania donovani - immunology ; Leishmaniasis, Visceral - diagnosis ; Male ; Middle Aged ; Visceral leishmaniasis ; Western blot</subject><ispartof>British journal of biomedical science, 2003-01, Vol.60 (4), p.210-216</ispartof><rights>Copyright 2003 Taylor and Francis Group LLC 2003</rights><rights>Copyright Step Publishing Ltd. 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-bf88d3aa1d2ab787599bcd381f78344cb1e99008321d639b91cd090e6cdcd1583</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14725337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Malla, N.</creatorcontrib><creatorcontrib>Sengupta, C.</creatorcontrib><creatorcontrib>Dubey, M.L.</creatorcontrib><creatorcontrib>Sud, A.</creatorcontrib><creatorcontrib>Ansari, N.A.</creatorcontrib><creatorcontrib>Salotra, P.</creatorcontrib><title>Antigenaemia and antibody response to Leishmania donovani stage-specific antigens and rk39 antigen in human immunodeficiency virus-infected patients</title><title>British journal of biomedical science</title><addtitle>Br J Biomed Sci</addtitle><description>In order to define the possible markers for the early diagnosis of asymptomatic visceral leishmaniasis in human immunodeficiency virus (HIV)-infected individuals, the antigenaemia and antibody response to stage-specific Leishmania donovani and rk39 antigens is assessed by enzyme-linked immunosorbent assay (ELISA) and immunoreactivity to stage-specific antigens analysed by Western blot. Serum samples from two out of 100 HIV-infected individuals were found positive for antigenaemia, antibody response to stage-specific L. donovani antigens and rk39 antigen, and one sample was also positive for antigenaemia and antibody response to L. donovani antigens, while antibody detection to rk39 antigen was not carried on this sample. Additionally, one sample was found positive for amastigote antigenaemia and antibody response to amastigote antigen, while in this patient promastigote antigenaemia and antibody response to promastigote L. donovani and rk39 antigen could not be detected. One sample was found positive for antigenaemia, antibody response to amastigote antigen and negative for antibody response to promastigote antigen, while in this patient response to rk39 antigen was borderline. Although antibody response to rk39 antigen could be detected in 9/88 (10%) HIV-infected individuals, in six of these nine patients neither antigenaemia nor antibody response to stage-specific L. donovani antigens could be detected. All 10 confirmed visceral leishmaniasis and HIV-negative control patients had positive antigenaemia and antibody response to L. donovani amastigote and promastigote antigens, while all the normal healthy individuals were negative. The study indicated that detection of antibody response to rk39 antigen, amastigote antigenaemia and antibody response to amastigote antigen may prove to be better markers than detection of promastigote antigenaemia, antibody response to promastigote antigen and immunoblot reactivity.</description><subject>Adolescent</subject><subject>Adult</subject><subject>AIDS-Related Opportunistic Infections - diagnosis</subject><subject>Animals</subject><subject>Antibodies, Protozoan - biosynthesis</subject><subject>Antigens, Protozoan - blood</subject><subject>Female</subject><subject>HIV-1</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - immunology</subject><subject>Leishmaniasis, Visceral - diagnosis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Visceral leishmaniasis</subject><subject>Western blot</subject><issn>0967-4845</issn><issn>2474-0896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc2KFDEUhYMoTjv6CkNw4a7a_FRVkmUz-AcNbnQdUsmtmYxVSZukRvo9fGBT090Iblxccrl859xwD0I3lGwpkeQ9Ub1oZdttGSF8S6mQXBD6DG1YK9qGSNU_R5sValbqCr3K-YEQqpjoX6Ir2grWcS426PcuFH8HwcDsDTbB1Sp-iO6IE-RDDBlwiXgPPt_PJlTGxRAfa4dzMXfQ5ANYP3r7pKtG-ckk_eDqMsE-4PulirGf5yVEBxX3EOwRP_q05MaHEWwBhw-m1HnJr9GL0UwZ3pzfa_T944dvt5-b_ddPX253-8ZyxUozjFI6bgx1zAxCik6pwTou6Viv0bZ2oKAUIZIz6nquBkWtI4pAb511tJP8Gr07-R5S_LlALnr22cI0mQBxyVrQjjDathV8-w_4EJcU6t80Y0LKTtLVrT9BNsWcE4z6kPxs0lFTotfQ9CU0vYamL6FV4c3ZfRlmcH9l55QqsDsB9VIxzeZXTJPTxRynmMZkgvVZ8_8s-QMXT6li</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Malla, N.</creator><creator>Sengupta, C.</creator><creator>Dubey, M.L.</creator><creator>Sud, A.</creator><creator>Ansari, N.A.</creator><creator>Salotra, P.</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Antigenaemia and antibody response to Leishmania donovani stage-specific antigens and rk39 antigen in human immunodeficiency virus-infected patients</title><author>Malla, N. ; Sengupta, C. ; Dubey, M.L. ; Sud, A. ; Ansari, N.A. ; Salotra, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-bf88d3aa1d2ab787599bcd381f78344cb1e99008321d639b91cd090e6cdcd1583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>AIDS-Related Opportunistic Infections - diagnosis</topic><topic>Animals</topic><topic>Antibodies, Protozoan - biosynthesis</topic><topic>Antigens, Protozoan - blood</topic><topic>Female</topic><topic>HIV-1</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - immunology</topic><topic>Leishmaniasis, Visceral - diagnosis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Visceral leishmaniasis</topic><topic>Western blot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Malla, N.</creatorcontrib><creatorcontrib>Sengupta, C.</creatorcontrib><creatorcontrib>Dubey, M.L.</creatorcontrib><creatorcontrib>Sud, A.</creatorcontrib><creatorcontrib>Ansari, N.A.</creatorcontrib><creatorcontrib>Salotra, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of biomedical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malla, N.</au><au>Sengupta, C.</au><au>Dubey, M.L.</au><au>Sud, A.</au><au>Ansari, N.A.</au><au>Salotra, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigenaemia and antibody response to Leishmania donovani stage-specific antigens and rk39 antigen in human immunodeficiency virus-infected patients</atitle><jtitle>British journal of biomedical science</jtitle><addtitle>Br J Biomed Sci</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>60</volume><issue>4</issue><spage>210</spage><epage>216</epage><pages>210-216</pages><issn>0967-4845</issn><eissn>2474-0896</eissn><abstract>In order to define the possible markers for the early diagnosis of asymptomatic visceral leishmaniasis in human immunodeficiency virus (HIV)-infected individuals, the antigenaemia and antibody response to stage-specific Leishmania donovani and rk39 antigens is assessed by enzyme-linked immunosorbent assay (ELISA) and immunoreactivity to stage-specific antigens analysed by Western blot. Serum samples from two out of 100 HIV-infected individuals were found positive for antigenaemia, antibody response to stage-specific L. donovani antigens and rk39 antigen, and one sample was also positive for antigenaemia and antibody response to L. donovani antigens, while antibody detection to rk39 antigen was not carried on this sample. Additionally, one sample was found positive for amastigote antigenaemia and antibody response to amastigote antigen, while in this patient promastigote antigenaemia and antibody response to promastigote L. donovani and rk39 antigen could not be detected. One sample was found positive for antigenaemia, antibody response to amastigote antigen and negative for antibody response to promastigote antigen, while in this patient response to rk39 antigen was borderline. Although antibody response to rk39 antigen could be detected in 9/88 (10%) HIV-infected individuals, in six of these nine patients neither antigenaemia nor antibody response to stage-specific L. donovani antigens could be detected. All 10 confirmed visceral leishmaniasis and HIV-negative control patients had positive antigenaemia and antibody response to L. donovani amastigote and promastigote antigens, while all the normal healthy individuals were negative. The study indicated that detection of antibody response to rk39 antigen, amastigote antigenaemia and antibody response to amastigote antigen may prove to be better markers than detection of promastigote antigenaemia, antibody response to promastigote antigen and immunoblot reactivity.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>14725337</pmid><doi>10.1080/09674845.2003.11783701</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Adult AIDS-Related Opportunistic Infections - diagnosis Animals Antibodies, Protozoan - biosynthesis Antigens, Protozoan - blood Female HIV-1 Human immunodeficiency virus Humans Leishmania donovani Leishmania donovani - immunology Leishmaniasis, Visceral - diagnosis Male Middle Aged Visceral leishmaniasis Western blot |
| title | Antigenaemia and antibody response to Leishmania donovani stage-specific antigens and rk39 antigen in human immunodeficiency virus-infected patients |
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