Evaluation of Phytochemicals, Antioxidants, Trace Elements in Kigelia africana Fruit Extracts and Chemical Profiling Analysis Using UHPLC-qTOF-MS2 Spectrometry
The study aimed at evaluating the phytochemical composition, antioxidant potentials and the levels of trace elements in the fruit extract of Kigelia africana obtained by different extraction solvents in order to ascertain its numerous pharmacological activities and identify the different chemical co...
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| Veröffentlicht in: | Biological trace element research 2020-06, Vol.195 (2), p.679-695 |
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| creator | Fagbohun, Oladapo F. Babalola, Olusegun O. Agboola, Femi K. Joseph, Jitcy S. Malindisa, Sibusiso Msagati, Titus A. M. |
| description | The study aimed at evaluating the phytochemical composition, antioxidant potentials and the levels of trace elements in the fruit extract of
Kigelia africana
obtained by different extraction solvents in order to ascertain its numerous pharmacological activities and identify the different chemical compounds responsible for these activities. The crude extract in ethanol and four other solvent fractions (hexane, ethylacetate, butanol and aqueous) were obtained for phytochemical screening. Antioxidant potentials of
K
.
africana
fruit were investigated spectrophotometrically using hydroxyl ion scavenging (OH
−
) activity, metal ion chelating activity, anti-lipid peroxidation activity as well as total antioxidant capacity assays. Trace element (Mn, Zn, Cd, Ni, Cu, Pb, Cr, Co and Fe) levels were measured using a plasma-emission spectrometer that has an auto sampler AS 93-plus and coupled with Nebulizer CETAC U-6000AT
+
after microwave acid digestion of the fruit extracts. Chemical identification was performed using ultra-high-pressure liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-qTOF-MS
2
).
Kigelia africana
fruit extracts obtained showed a variety of bioactive phytochemical compounds including phenolic acids, flavonoids, saponins, tannins and glycosides. The total antioxidant capacity activities of the aqueous, butanol, ethanol, hexane and ethylacetate extracts are 15.04, 52.11, 44.95, 79.27 and 175.20 mg AAE/g. Metal ion chelating activity showed significant correlation with lipid peroxidation inhibition activity at
p
≤ 0.01 and with OH
−
scavenging activity at
p
≤ 0.05. PCA analysis revealed that all the extract/fractions have higher total antioxidant activities compared to aqueous extract with hexane extract exhibiting the highest radical scavenging potential. HCA showed similarities with three well-defined clusters and PLS regression was used to predict total antioxidant activity. High sensitivity by low values of limits of detection and quantification was observed ranging from 0.021 to 0.085 mg/ml and 0.063 to 0.258 mg/ml for Zn and Fe respectively. Ethylacetate extract had high concentration of Fe (0.5656 mg/kg). For the standardization of the
K
.
africana
fruit extract, 244 chemical compounds were identified by measuring
m
/
z
values with threshold override of 100,000 and analysing mass spectrometer fragmentation behaviour while 16 of these were confirmed.
Kigelia africana
fruit extract is a good source of antioxidant |
| doi_str_mv | 10.1007/s12011-019-01869-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2276693732</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2276693732</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-1c72d4b92cd5043c9094ab31027ed2e6af38d930ba6a457bec6e669df96b08a03</originalsourceid><addsrcrecordid>eNp9kc1KAzEUhYMoWKsv4Crg1tH8zF-WpUytWGmh7TpkMpk2ZTrTJhnpPI2vauoU3Lm4XA73O4cLB4BHjF4wQsmrxQRhHCDM_KQxC8gVGOAo8jIh6BoMEI5pELI0vAV31u4QwglhdAC-sy9RtcLppoZNCRfbzjVyq_Zaiso-w1HtLyddiNp5tTJCKphVaq-8hrqGH3qjKi2gKI131AJOTKsdzE7Oox4RdQHHlzi4ME2pK11vfKyoOqstXNuzXE8Xs3FwXM0nweeSwOVBSWeavXKmuwc3pf9EPVz2EKwn2Wo8DWbzt_fxaBZIkhIXYJmQIswZkUWEQioZYqHIKUYkUQVRsShpWjCKchGLMEpyJWMVx6woWZyjVCA6BE997sE0x1ZZx3dNa_yblhOSeJQmlHiK9JQ0jbVGlfxg9F6YjmPEz0Xwvgjui-C_RfCzifYm6-F6o8xf9D-uH9vFjRE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2276693732</pqid></control><display><type>article</type><title>Evaluation of Phytochemicals, Antioxidants, Trace Elements in Kigelia africana Fruit Extracts and Chemical Profiling Analysis Using UHPLC-qTOF-MS2 Spectrometry</title><source>SpringerLink Journals</source><creator>Fagbohun, Oladapo F. ; Babalola, Olusegun O. ; Agboola, Femi K. ; Joseph, Jitcy S. ; Malindisa, Sibusiso ; Msagati, Titus A. M.</creator><creatorcontrib>Fagbohun, Oladapo F. ; Babalola, Olusegun O. ; Agboola, Femi K. ; Joseph, Jitcy S. ; Malindisa, Sibusiso ; Msagati, Titus A. M.</creatorcontrib><description>The study aimed at evaluating the phytochemical composition, antioxidant potentials and the levels of trace elements in the fruit extract of
Kigelia africana
obtained by different extraction solvents in order to ascertain its numerous pharmacological activities and identify the different chemical compounds responsible for these activities. The crude extract in ethanol and four other solvent fractions (hexane, ethylacetate, butanol and aqueous) were obtained for phytochemical screening. Antioxidant potentials of
K
.
africana
fruit were investigated spectrophotometrically using hydroxyl ion scavenging (OH
−
) activity, metal ion chelating activity, anti-lipid peroxidation activity as well as total antioxidant capacity assays. Trace element (Mn, Zn, Cd, Ni, Cu, Pb, Cr, Co and Fe) levels were measured using a plasma-emission spectrometer that has an auto sampler AS 93-plus and coupled with Nebulizer CETAC U-6000AT
+
after microwave acid digestion of the fruit extracts. Chemical identification was performed using ultra-high-pressure liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-qTOF-MS
2
).
Kigelia africana
fruit extracts obtained showed a variety of bioactive phytochemical compounds including phenolic acids, flavonoids, saponins, tannins and glycosides. The total antioxidant capacity activities of the aqueous, butanol, ethanol, hexane and ethylacetate extracts are 15.04, 52.11, 44.95, 79.27 and 175.20 mg AAE/g. Metal ion chelating activity showed significant correlation with lipid peroxidation inhibition activity at
p
≤ 0.01 and with OH
−
scavenging activity at
p
≤ 0.05. PCA analysis revealed that all the extract/fractions have higher total antioxidant activities compared to aqueous extract with hexane extract exhibiting the highest radical scavenging potential. HCA showed similarities with three well-defined clusters and PLS regression was used to predict total antioxidant activity. High sensitivity by low values of limits of detection and quantification was observed ranging from 0.021 to 0.085 mg/ml and 0.063 to 0.258 mg/ml for Zn and Fe respectively. Ethylacetate extract had high concentration of Fe (0.5656 mg/kg). For the standardization of the
K
.
africana
fruit extract, 244 chemical compounds were identified by measuring
m
/
z
values with threshold override of 100,000 and analysing mass spectrometer fragmentation behaviour while 16 of these were confirmed.
Kigelia africana
fruit extract is a good source of antioxidant and possess maximum accepted concentration of trace elements according to European legislation (1881/2006/EC). The metabolites identified exhibited numerous pharmacological activities. The method and results suggest the applicability for commercial use of this
K
.
africana
fruit in the treatment of oxidative-related diseases.
Graphical abstract
The phytochemical, antioxidant and trace element composition of crude ethanol extract, hexane, butanol, aqueous and ethylacetate extracts of
Kigelia africana
fruit were determined. The fruit extracts were found to possess good antioxidant activity, maximum acceptable amount of essential trace elements as well as the presence of bioactive phytochemicals.
K. africana
fruit would be an ideal candidate in improving human health and thus the management of oxidative-related diseases such as diabetes, by involving in the antioxidant defense system against free radical generation.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-019-01869-2</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acid digestion ; Antioxidants ; Biochemistry ; Biological activity ; Biomedical and Life Sciences ; Biotechnology ; Butanol ; Cadmium ; Chelation ; Chemical compounds ; Chromium ; Composition ; Copper ; Detection ; Diabetes mellitus ; Diseases ; Emission measurements ; Ethanol ; Flavonoids ; Free radicals ; Fruits ; Glycosides ; Heavy metals ; High-performance liquid chromatography ; Identification ; Iron ; Kigelia africana ; Legislation ; Levels ; Life Sciences ; Lipid peroxidation ; Lipids ; Liquid chromatography ; Manganese ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Metal ions ; Nickel ; Nutrition ; Oncology ; Organic chemistry ; Peroxidation ; Pharmacology ; Phenolic acids ; Phenols ; Phytochemicals ; Regression analysis ; Saponins ; Scavenging ; Scientific imaging ; Solvent extraction ; Solvents ; Spectrophotometry ; Spectroscopy ; Standardization ; Trace elements ; Zinc</subject><ispartof>Biological trace element research, 2020-06, Vol.195 (2), p.679-695</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-1c72d4b92cd5043c9094ab31027ed2e6af38d930ba6a457bec6e669df96b08a03</citedby><cites>FETCH-LOGICAL-c282t-1c72d4b92cd5043c9094ab31027ed2e6af38d930ba6a457bec6e669df96b08a03</cites><orcidid>0000-0002-1340-6697</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-019-01869-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-019-01869-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Fagbohun, Oladapo F.</creatorcontrib><creatorcontrib>Babalola, Olusegun O.</creatorcontrib><creatorcontrib>Agboola, Femi K.</creatorcontrib><creatorcontrib>Joseph, Jitcy S.</creatorcontrib><creatorcontrib>Malindisa, Sibusiso</creatorcontrib><creatorcontrib>Msagati, Titus A. M.</creatorcontrib><title>Evaluation of Phytochemicals, Antioxidants, Trace Elements in Kigelia africana Fruit Extracts and Chemical Profiling Analysis Using UHPLC-qTOF-MS2 Spectrometry</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><description>The study aimed at evaluating the phytochemical composition, antioxidant potentials and the levels of trace elements in the fruit extract of
Kigelia africana
obtained by different extraction solvents in order to ascertain its numerous pharmacological activities and identify the different chemical compounds responsible for these activities. The crude extract in ethanol and four other solvent fractions (hexane, ethylacetate, butanol and aqueous) were obtained for phytochemical screening. Antioxidant potentials of
K
.
africana
fruit were investigated spectrophotometrically using hydroxyl ion scavenging (OH
−
) activity, metal ion chelating activity, anti-lipid peroxidation activity as well as total antioxidant capacity assays. Trace element (Mn, Zn, Cd, Ni, Cu, Pb, Cr, Co and Fe) levels were measured using a plasma-emission spectrometer that has an auto sampler AS 93-plus and coupled with Nebulizer CETAC U-6000AT
+
after microwave acid digestion of the fruit extracts. Chemical identification was performed using ultra-high-pressure liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-qTOF-MS
2
).
Kigelia africana
fruit extracts obtained showed a variety of bioactive phytochemical compounds including phenolic acids, flavonoids, saponins, tannins and glycosides. The total antioxidant capacity activities of the aqueous, butanol, ethanol, hexane and ethylacetate extracts are 15.04, 52.11, 44.95, 79.27 and 175.20 mg AAE/g. Metal ion chelating activity showed significant correlation with lipid peroxidation inhibition activity at
p
≤ 0.01 and with OH
−
scavenging activity at
p
≤ 0.05. PCA analysis revealed that all the extract/fractions have higher total antioxidant activities compared to aqueous extract with hexane extract exhibiting the highest radical scavenging potential. HCA showed similarities with three well-defined clusters and PLS regression was used to predict total antioxidant activity. High sensitivity by low values of limits of detection and quantification was observed ranging from 0.021 to 0.085 mg/ml and 0.063 to 0.258 mg/ml for Zn and Fe respectively. Ethylacetate extract had high concentration of Fe (0.5656 mg/kg). For the standardization of the
K
.
africana
fruit extract, 244 chemical compounds were identified by measuring
m
/
z
values with threshold override of 100,000 and analysing mass spectrometer fragmentation behaviour while 16 of these were confirmed.
Kigelia africana
fruit extract is a good source of antioxidant and possess maximum accepted concentration of trace elements according to European legislation (1881/2006/EC). The metabolites identified exhibited numerous pharmacological activities. The method and results suggest the applicability for commercial use of this
K
.
africana
fruit in the treatment of oxidative-related diseases.
Graphical abstract
The phytochemical, antioxidant and trace element composition of crude ethanol extract, hexane, butanol, aqueous and ethylacetate extracts of
Kigelia africana
fruit were determined. The fruit extracts were found to possess good antioxidant activity, maximum acceptable amount of essential trace elements as well as the presence of bioactive phytochemicals.
K. africana
fruit would be an ideal candidate in improving human health and thus the management of oxidative-related diseases such as diabetes, by involving in the antioxidant defense system against free radical generation.</description><subject>Acid digestion</subject><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Butanol</subject><subject>Cadmium</subject><subject>Chelation</subject><subject>Chemical compounds</subject><subject>Chromium</subject><subject>Composition</subject><subject>Copper</subject><subject>Detection</subject><subject>Diabetes mellitus</subject><subject>Diseases</subject><subject>Emission measurements</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Free radicals</subject><subject>Fruits</subject><subject>Glycosides</subject><subject>Heavy metals</subject><subject>High-performance liquid chromatography</subject><subject>Identification</subject><subject>Iron</subject><subject>Kigelia africana</subject><subject>Legislation</subject><subject>Levels</subject><subject>Life Sciences</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>Manganese</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Metal ions</subject><subject>Nickel</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Organic chemistry</subject><subject>Peroxidation</subject><subject>Pharmacology</subject><subject>Phenolic acids</subject><subject>Phenols</subject><subject>Phytochemicals</subject><subject>Regression analysis</subject><subject>Saponins</subject><subject>Scavenging</subject><subject>Scientific imaging</subject><subject>Solvent extraction</subject><subject>Solvents</subject><subject>Spectrophotometry</subject><subject>Spectroscopy</subject><subject>Standardization</subject><subject>Trace elements</subject><subject>Zinc</subject><issn>0163-4984</issn><issn>1559-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1KAzEUhYMoWKsv4Crg1tH8zF-WpUytWGmh7TpkMpk2ZTrTJhnpPI2vauoU3Lm4XA73O4cLB4BHjF4wQsmrxQRhHCDM_KQxC8gVGOAo8jIh6BoMEI5pELI0vAV31u4QwglhdAC-sy9RtcLppoZNCRfbzjVyq_Zaiso-w1HtLyddiNp5tTJCKphVaq-8hrqGH3qjKi2gKI131AJOTKsdzE7Oox4RdQHHlzi4ME2pK11vfKyoOqstXNuzXE8Xs3FwXM0nweeSwOVBSWeavXKmuwc3pf9EPVz2EKwn2Wo8DWbzt_fxaBZIkhIXYJmQIswZkUWEQioZYqHIKUYkUQVRsShpWjCKchGLMEpyJWMVx6woWZyjVCA6BE997sE0x1ZZx3dNa_yblhOSeJQmlHiK9JQ0jbVGlfxg9F6YjmPEz0Xwvgjui-C_RfCzifYm6-F6o8xf9D-uH9vFjRE</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Fagbohun, Oladapo F.</creator><creator>Babalola, Olusegun O.</creator><creator>Agboola, Femi K.</creator><creator>Joseph, Jitcy S.</creator><creator>Malindisa, Sibusiso</creator><creator>Msagati, Titus A. M.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><orcidid>https://orcid.org/0000-0002-1340-6697</orcidid></search><sort><creationdate>20200601</creationdate><title>Evaluation of Phytochemicals, Antioxidants, Trace Elements in Kigelia africana Fruit Extracts and Chemical Profiling Analysis Using UHPLC-qTOF-MS2 Spectrometry</title><author>Fagbohun, Oladapo F. ; Babalola, Olusegun O. ; Agboola, Femi K. ; Joseph, Jitcy S. ; Malindisa, Sibusiso ; Msagati, Titus A. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-1c72d4b92cd5043c9094ab31027ed2e6af38d930ba6a457bec6e669df96b08a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acid digestion</topic><topic>Antioxidants</topic><topic>Biochemistry</topic><topic>Biological activity</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Butanol</topic><topic>Cadmium</topic><topic>Chelation</topic><topic>Chemical compounds</topic><topic>Chromium</topic><topic>Composition</topic><topic>Copper</topic><topic>Detection</topic><topic>Diabetes mellitus</topic><topic>Diseases</topic><topic>Emission measurements</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>Free radicals</topic><topic>Fruits</topic><topic>Glycosides</topic><topic>Heavy metals</topic><topic>High-performance liquid chromatography</topic><topic>Identification</topic><topic>Iron</topic><topic>Kigelia africana</topic><topic>Legislation</topic><topic>Levels</topic><topic>Life Sciences</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Liquid chromatography</topic><topic>Manganese</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Metal ions</topic><topic>Nickel</topic><topic>Nutrition</topic><topic>Oncology</topic><topic>Organic chemistry</topic><topic>Peroxidation</topic><topic>Pharmacology</topic><topic>Phenolic acids</topic><topic>Phenols</topic><topic>Phytochemicals</topic><topic>Regression analysis</topic><topic>Saponins</topic><topic>Scavenging</topic><topic>Scientific imaging</topic><topic>Solvent extraction</topic><topic>Solvents</topic><topic>Spectrophotometry</topic><topic>Spectroscopy</topic><topic>Standardization</topic><topic>Trace elements</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fagbohun, Oladapo F.</creatorcontrib><creatorcontrib>Babalola, Olusegun O.</creatorcontrib><creatorcontrib>Agboola, Femi K.</creatorcontrib><creatorcontrib>Joseph, Jitcy S.</creatorcontrib><creatorcontrib>Malindisa, Sibusiso</creatorcontrib><creatorcontrib>Msagati, Titus A. M.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Biological trace element research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fagbohun, Oladapo F.</au><au>Babalola, Olusegun O.</au><au>Agboola, Femi K.</au><au>Joseph, Jitcy S.</au><au>Malindisa, Sibusiso</au><au>Msagati, Titus A. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Phytochemicals, Antioxidants, Trace Elements in Kigelia africana Fruit Extracts and Chemical Profiling Analysis Using UHPLC-qTOF-MS2 Spectrometry</atitle><jtitle>Biological trace element research</jtitle><stitle>Biol Trace Elem Res</stitle><date>2020-06-01</date><risdate>2020</risdate><volume>195</volume><issue>2</issue><spage>679</spage><epage>695</epage><pages>679-695</pages><issn>0163-4984</issn><eissn>1559-0720</eissn><abstract>The study aimed at evaluating the phytochemical composition, antioxidant potentials and the levels of trace elements in the fruit extract of
Kigelia africana
obtained by different extraction solvents in order to ascertain its numerous pharmacological activities and identify the different chemical compounds responsible for these activities. The crude extract in ethanol and four other solvent fractions (hexane, ethylacetate, butanol and aqueous) were obtained for phytochemical screening. Antioxidant potentials of
K
.
africana
fruit were investigated spectrophotometrically using hydroxyl ion scavenging (OH
−
) activity, metal ion chelating activity, anti-lipid peroxidation activity as well as total antioxidant capacity assays. Trace element (Mn, Zn, Cd, Ni, Cu, Pb, Cr, Co and Fe) levels were measured using a plasma-emission spectrometer that has an auto sampler AS 93-plus and coupled with Nebulizer CETAC U-6000AT
+
after microwave acid digestion of the fruit extracts. Chemical identification was performed using ultra-high-pressure liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-qTOF-MS
2
).
Kigelia africana
fruit extracts obtained showed a variety of bioactive phytochemical compounds including phenolic acids, flavonoids, saponins, tannins and glycosides. The total antioxidant capacity activities of the aqueous, butanol, ethanol, hexane and ethylacetate extracts are 15.04, 52.11, 44.95, 79.27 and 175.20 mg AAE/g. Metal ion chelating activity showed significant correlation with lipid peroxidation inhibition activity at
p
≤ 0.01 and with OH
−
scavenging activity at
p
≤ 0.05. PCA analysis revealed that all the extract/fractions have higher total antioxidant activities compared to aqueous extract with hexane extract exhibiting the highest radical scavenging potential. HCA showed similarities with three well-defined clusters and PLS regression was used to predict total antioxidant activity. High sensitivity by low values of limits of detection and quantification was observed ranging from 0.021 to 0.085 mg/ml and 0.063 to 0.258 mg/ml for Zn and Fe respectively. Ethylacetate extract had high concentration of Fe (0.5656 mg/kg). For the standardization of the
K
.
africana
fruit extract, 244 chemical compounds were identified by measuring
m
/
z
values with threshold override of 100,000 and analysing mass spectrometer fragmentation behaviour while 16 of these were confirmed.
Kigelia africana
fruit extract is a good source of antioxidant and possess maximum accepted concentration of trace elements according to European legislation (1881/2006/EC). The metabolites identified exhibited numerous pharmacological activities. The method and results suggest the applicability for commercial use of this
K
.
africana
fruit in the treatment of oxidative-related diseases.
Graphical abstract
The phytochemical, antioxidant and trace element composition of crude ethanol extract, hexane, butanol, aqueous and ethylacetate extracts of
Kigelia africana
fruit were determined. The fruit extracts were found to possess good antioxidant activity, maximum acceptable amount of essential trace elements as well as the presence of bioactive phytochemicals.
K. africana
fruit would be an ideal candidate in improving human health and thus the management of oxidative-related diseases such as diabetes, by involving in the antioxidant defense system against free radical generation.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12011-019-01869-2</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-1340-6697</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0163-4984 |
| ispartof | Biological trace element research, 2020-06, Vol.195 (2), p.679-695 |
| issn | 0163-4984 1559-0720 |
| language | eng |
| recordid | cdi_proquest_journals_2276693732 |
| source | SpringerLink Journals |
| subjects | Acid digestion Antioxidants Biochemistry Biological activity Biomedical and Life Sciences Biotechnology Butanol Cadmium Chelation Chemical compounds Chromium Composition Copper Detection Diabetes mellitus Diseases Emission measurements Ethanol Flavonoids Free radicals Fruits Glycosides Heavy metals High-performance liquid chromatography Identification Iron Kigelia africana Legislation Levels Life Sciences Lipid peroxidation Lipids Liquid chromatography Manganese Mass spectrometry Mass spectroscopy Metabolites Metal ions Nickel Nutrition Oncology Organic chemistry Peroxidation Pharmacology Phenolic acids Phenols Phytochemicals Regression analysis Saponins Scavenging Scientific imaging Solvent extraction Solvents Spectrophotometry Spectroscopy Standardization Trace elements Zinc |
| title | Evaluation of Phytochemicals, Antioxidants, Trace Elements in Kigelia africana Fruit Extracts and Chemical Profiling Analysis Using UHPLC-qTOF-MS2 Spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T16%3A50%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20Phytochemicals,%20Antioxidants,%20Trace%20Elements%20in%20Kigelia%20africana%20Fruit%20Extracts%20and%20Chemical%20Profiling%20Analysis%20Using%20UHPLC-qTOF-MS2%20Spectrometry&rft.jtitle=Biological%20trace%20element%20research&rft.au=Fagbohun,%20Oladapo%20F.&rft.date=2020-06-01&rft.volume=195&rft.issue=2&rft.spage=679&rft.epage=695&rft.pages=679-695&rft.issn=0163-4984&rft.eissn=1559-0720&rft_id=info:doi/10.1007/s12011-019-01869-2&rft_dat=%3Cproquest_cross%3E2276693732%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2276693732&rft_id=info:pmid/&rfr_iscdi=true |