The tumor suppressor protein menin interacts with NF-[kappa]B proteins and inhibits NF-[kappa]B-mediated transactivation
Multiple endocrine neoplasia type 1 is an autosomal dominant tumor syndrome. Manifestations include neoplasms of the parathyroid glands, enteropancreatic neuroendocrine cells, and the anterior pituitary gland. The MEN1 tumor suppressor gene encodes menin, a 610 amino acid nuclear protein without seq...
Gespeichert in:
| Veröffentlicht in: | Oncogene 2001-08, Vol.20 (36), p.4917 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 36 |
| container_start_page | 4917 |
| container_title | Oncogene |
| container_volume | 20 |
| creator | Heppner, Christina Bilimoria, Karl Y Agarwal, Sunita K Kester, MaryBeth Whitty, Leslie J Guru, Siradanahalli C Chandrasekharappa, Settara C Collins, Francis S Spiegel, Allen M Marx, Stephen J A Lee Burns |
| description | Multiple endocrine neoplasia type 1 is an autosomal dominant tumor syndrome. Manifestations include neoplasms of the parathyroid glands, enteropancreatic neuroendocrine cells, and the anterior pituitary gland. The MEN1 tumor suppressor gene encodes menin, a 610 amino acid nuclear protein without sequence homology to other proteins. To elucidate menin function, we used immunoprecipitation to identify interacting proteins. The NF-kappaB proteins p50, p52 and p65 were found to interact specifically and directly with menin in vitro and in vivo. The region of NF-kappaB proteins sufficient for binding to menin is the N-terminus. Furthermore, amino acids 305-381 of menin are essential for this binding. Menin represses p65-mediated transcriptional activation on NF-kappaB sites in a dose-dependent and specific manner. Also, PMA (phorbol 12-myristate 13-acetate)-stimulated NF-kappaB activation is suppressed by menin. These observations suggest that menin's ability to interact with NF-kappaB proteins and its modulation of NF-kappaB transactivation contribute to menin's tumor suppressor function. |
| doi_str_mv | 10.1038/sj.onc.1204529 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_227322933</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1012933591</sourcerecordid><originalsourceid>FETCH-proquest_journals_2273229333</originalsourceid><addsrcrecordid>eNqNzDFPwzAQBWALgUSgrMwWu4NzTgheQVRMnbohVLnNoTgQ2_guwM_HA0iMLO-edJ-eEJeNrhttbq9pqmM41A3otgN7JKqm7W9U19n2WFTadlpZMHAqzogmrXVvNVTiazui5GWOWdKSUkaiUlOOjD7IGUNJHxizOzDJT8-j3KzV06tLyT3f_UKSLgzFjX7vC_sj1IyDd4yD5OwClRX_4djHsBInL-6N8OLnnour9cP2_lGVyfcFiXdTXHIorx1AbwCsMeZf6BsmZ1Ta</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>227322933</pqid></control><display><type>article</type><title>The tumor suppressor protein menin interacts with NF-[kappa]B proteins and inhibits NF-[kappa]B-mediated transactivation</title><source>SpringerLink Journals</source><source>Nature Journals Online</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Heppner, Christina ; Bilimoria, Karl Y ; Agarwal, Sunita K ; Kester, MaryBeth ; Whitty, Leslie J ; Guru, Siradanahalli C ; Chandrasekharappa, Settara C ; Collins, Francis S ; Spiegel, Allen M ; Marx, Stephen J ; A Lee Burns</creator><creatorcontrib>Heppner, Christina ; Bilimoria, Karl Y ; Agarwal, Sunita K ; Kester, MaryBeth ; Whitty, Leslie J ; Guru, Siradanahalli C ; Chandrasekharappa, Settara C ; Collins, Francis S ; Spiegel, Allen M ; Marx, Stephen J ; A Lee Burns</creatorcontrib><description>Multiple endocrine neoplasia type 1 is an autosomal dominant tumor syndrome. Manifestations include neoplasms of the parathyroid glands, enteropancreatic neuroendocrine cells, and the anterior pituitary gland. The MEN1 tumor suppressor gene encodes menin, a 610 amino acid nuclear protein without sequence homology to other proteins. To elucidate menin function, we used immunoprecipitation to identify interacting proteins. The NF-kappaB proteins p50, p52 and p65 were found to interact specifically and directly with menin in vitro and in vivo. The region of NF-kappaB proteins sufficient for binding to menin is the N-terminus. Furthermore, amino acids 305-381 of menin are essential for this binding. Menin represses p65-mediated transcriptional activation on NF-kappaB sites in a dose-dependent and specific manner. Also, PMA (phorbol 12-myristate 13-acetate)-stimulated NF-kappaB activation is suppressed by menin. These observations suggest that menin's ability to interact with NF-kappaB proteins and its modulation of NF-kappaB transactivation contribute to menin's tumor suppressor function.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1204529</identifier><identifier>CODEN: ONCNES</identifier><language>eng</language><publisher>New York: Nature Publishing Group</publisher><subject>Amino acids ; Antibodies ; Molecular weight ; Mutation ; Proteins ; Transcription factors ; Tumors</subject><ispartof>Oncogene, 2001-08, Vol.20 (36), p.4917</ispartof><rights>Copyright Nature Publishing Group Aug 16, 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Heppner, Christina</creatorcontrib><creatorcontrib>Bilimoria, Karl Y</creatorcontrib><creatorcontrib>Agarwal, Sunita K</creatorcontrib><creatorcontrib>Kester, MaryBeth</creatorcontrib><creatorcontrib>Whitty, Leslie J</creatorcontrib><creatorcontrib>Guru, Siradanahalli C</creatorcontrib><creatorcontrib>Chandrasekharappa, Settara C</creatorcontrib><creatorcontrib>Collins, Francis S</creatorcontrib><creatorcontrib>Spiegel, Allen M</creatorcontrib><creatorcontrib>Marx, Stephen J</creatorcontrib><creatorcontrib>A Lee Burns</creatorcontrib><title>The tumor suppressor protein menin interacts with NF-[kappa]B proteins and inhibits NF-[kappa]B-mediated transactivation</title><title>Oncogene</title><description>Multiple endocrine neoplasia type 1 is an autosomal dominant tumor syndrome. Manifestations include neoplasms of the parathyroid glands, enteropancreatic neuroendocrine cells, and the anterior pituitary gland. The MEN1 tumor suppressor gene encodes menin, a 610 amino acid nuclear protein without sequence homology to other proteins. To elucidate menin function, we used immunoprecipitation to identify interacting proteins. The NF-kappaB proteins p50, p52 and p65 were found to interact specifically and directly with menin in vitro and in vivo. The region of NF-kappaB proteins sufficient for binding to menin is the N-terminus. Furthermore, amino acids 305-381 of menin are essential for this binding. Menin represses p65-mediated transcriptional activation on NF-kappaB sites in a dose-dependent and specific manner. Also, PMA (phorbol 12-myristate 13-acetate)-stimulated NF-kappaB activation is suppressed by menin. These observations suggest that menin's ability to interact with NF-kappaB proteins and its modulation of NF-kappaB transactivation contribute to menin's tumor suppressor function.</description><subject>Amino acids</subject><subject>Antibodies</subject><subject>Molecular weight</subject><subject>Mutation</subject><subject>Proteins</subject><subject>Transcription factors</subject><subject>Tumors</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNzDFPwzAQBWALgUSgrMwWu4NzTgheQVRMnbohVLnNoTgQ2_guwM_HA0iMLO-edJ-eEJeNrhttbq9pqmM41A3otgN7JKqm7W9U19n2WFTadlpZMHAqzogmrXVvNVTiazui5GWOWdKSUkaiUlOOjD7IGUNJHxizOzDJT8-j3KzV06tLyT3f_UKSLgzFjX7vC_sj1IyDd4yD5OwClRX_4djHsBInL-6N8OLnnour9cP2_lGVyfcFiXdTXHIorx1AbwCsMeZf6BsmZ1Ta</recordid><startdate>20010816</startdate><enddate>20010816</enddate><creator>Heppner, Christina</creator><creator>Bilimoria, Karl Y</creator><creator>Agarwal, Sunita K</creator><creator>Kester, MaryBeth</creator><creator>Whitty, Leslie J</creator><creator>Guru, Siradanahalli C</creator><creator>Chandrasekharappa, Settara C</creator><creator>Collins, Francis S</creator><creator>Spiegel, Allen M</creator><creator>Marx, Stephen J</creator><creator>A Lee Burns</creator><general>Nature Publishing Group</general><scope>3V.</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20010816</creationdate><title>The tumor suppressor protein menin interacts with NF-[kappa]B proteins and inhibits NF-[kappa]B-mediated transactivation</title><author>Heppner, Christina ; Bilimoria, Karl Y ; Agarwal, Sunita K ; Kester, MaryBeth ; Whitty, Leslie J ; Guru, Siradanahalli C ; Chandrasekharappa, Settara C ; Collins, Francis S ; Spiegel, Allen M ; Marx, Stephen J ; A Lee Burns</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_2273229333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino acids</topic><topic>Antibodies</topic><topic>Molecular weight</topic><topic>Mutation</topic><topic>Proteins</topic><topic>Transcription factors</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heppner, Christina</creatorcontrib><creatorcontrib>Bilimoria, Karl Y</creatorcontrib><creatorcontrib>Agarwal, Sunita K</creatorcontrib><creatorcontrib>Kester, MaryBeth</creatorcontrib><creatorcontrib>Whitty, Leslie J</creatorcontrib><creatorcontrib>Guru, Siradanahalli C</creatorcontrib><creatorcontrib>Chandrasekharappa, Settara C</creatorcontrib><creatorcontrib>Collins, Francis S</creatorcontrib><creatorcontrib>Spiegel, Allen M</creatorcontrib><creatorcontrib>Marx, Stephen J</creatorcontrib><creatorcontrib>A Lee Burns</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Oncogene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heppner, Christina</au><au>Bilimoria, Karl Y</au><au>Agarwal, Sunita K</au><au>Kester, MaryBeth</au><au>Whitty, Leslie J</au><au>Guru, Siradanahalli C</au><au>Chandrasekharappa, Settara C</au><au>Collins, Francis S</au><au>Spiegel, Allen M</au><au>Marx, Stephen J</au><au>A Lee Burns</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The tumor suppressor protein menin interacts with NF-[kappa]B proteins and inhibits NF-[kappa]B-mediated transactivation</atitle><jtitle>Oncogene</jtitle><date>2001-08-16</date><risdate>2001</risdate><volume>20</volume><issue>36</issue><spage>4917</spage><pages>4917-</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><coden>ONCNES</coden><abstract>Multiple endocrine neoplasia type 1 is an autosomal dominant tumor syndrome. Manifestations include neoplasms of the parathyroid glands, enteropancreatic neuroendocrine cells, and the anterior pituitary gland. The MEN1 tumor suppressor gene encodes menin, a 610 amino acid nuclear protein without sequence homology to other proteins. To elucidate menin function, we used immunoprecipitation to identify interacting proteins. The NF-kappaB proteins p50, p52 and p65 were found to interact specifically and directly with menin in vitro and in vivo. The region of NF-kappaB proteins sufficient for binding to menin is the N-terminus. Furthermore, amino acids 305-381 of menin are essential for this binding. Menin represses p65-mediated transcriptional activation on NF-kappaB sites in a dose-dependent and specific manner. Also, PMA (phorbol 12-myristate 13-acetate)-stimulated NF-kappaB activation is suppressed by menin. These observations suggest that menin's ability to interact with NF-kappaB proteins and its modulation of NF-kappaB transactivation contribute to menin's tumor suppressor function.</abstract><cop>New York</cop><pub>Nature Publishing Group</pub><doi>10.1038/sj.onc.1204529</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-9232 |
| ispartof | Oncogene, 2001-08, Vol.20 (36), p.4917 |
| issn | 0950-9232 1476-5594 |
| language | eng |
| recordid | cdi_proquest_journals_227322933 |
| source | SpringerLink Journals; Nature Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino acids Antibodies Molecular weight Mutation Proteins Transcription factors Tumors |
| title | The tumor suppressor protein menin interacts with NF-[kappa]B proteins and inhibits NF-[kappa]B-mediated transactivation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T01%3A23%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20tumor%20suppressor%20protein%20menin%20interacts%20with%20NF-%5Bkappa%5DB%20proteins%20and%20inhibits%20NF-%5Bkappa%5DB-mediated%20transactivation&rft.jtitle=Oncogene&rft.au=Heppner,%20Christina&rft.date=2001-08-16&rft.volume=20&rft.issue=36&rft.spage=4917&rft.pages=4917-&rft.issn=0950-9232&rft.eissn=1476-5594&rft.coden=ONCNES&rft_id=info:doi/10.1038/sj.onc.1204529&rft_dat=%3Cproquest%3E1012933591%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=227322933&rft_id=info:pmid/&rfr_iscdi=true |