Identification of amino acid substitutions that alter the substrate specificity of TEM-1 (beta)-lactamase

TEM-1 beta-lactamase is the most prevalent plasmid-mediated beta-lactamase in gram-negative bacteria. To identify amino acid substitutions that alter the activity of TEM-1 towards extended-spectrum cephalosporins, regions around the active-site pocket were probed by random-replacement mutagenesis.

Bibliographische Detailangaben

Veröffentlicht in:	Journal of bacteriology 1992-08, Vol.174 (16), p.5237
Hauptverfasser:	Palzkill, Timothy, Botstein, David
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics Bacteriology
Online-Zugang:	Volltext
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