Identification of amino acid substitutions that alter the substrate specificity of TEM-1 (beta)-lactamase

Identification of amino acid substitutions that alter the substrate specificity of TEM-1 (beta)-lactamase

TEM-1 beta-lactamase is the most prevalent plasmid-mediated beta-lactamase in gram-negative bacteria. To identify amino acid substitutions that alter the activity of TEM-1 towards extended-spectrum cephalosporins, regions around the active-site pocket were probed by random-replacement mutagenesis.

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Veröffentlicht in:Journal of bacteriology 1992-08, Vol.174 (16), p.5237
Hauptverfasser: Palzkill, Timothy, Botstein, David
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Sprache:eng
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Antibiotics
Bacteriology
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container_title Journal of bacteriology
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creator Palzkill, Timothy
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description TEM-1 beta-lactamase is the most prevalent plasmid-mediated beta-lactamase in gram-negative bacteria. To identify amino acid substitutions that alter the activity of TEM-1 towards extended-spectrum cephalosporins, regions around the active-site pocket were probed by random-replacement mutagenesis.
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Antibiotics
Bacteriology
title Identification of amino acid substitutions that alter the substrate specificity of TEM-1 (beta)-lactamase
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