Nitrogen-doped graphene quantum dots doped silica nanoparticles as enhancers for electrochemiluminescence thrombin aptasensors based on 3D graphene
A sensitive electrochemiluminescence (ECL) aptasensor based on 3D graphene is described for thrombin detection utilizing nitrogen-doped graphene quantum dots doped silica (NGQDs@SiO 2 ) nanocomposite labeling as an enhancer. Ru(bpy) 3 2+ -Pt nanoparticles (Ru-PtNPs) with an ECL signal were dropped o...
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| Veröffentlicht in: | Journal of solid state electrochemistry 2019-08, Vol.23 (8), p.2579-2588 |
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| container_title | Journal of solid state electrochemistry |
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| creator | Du, Fangkai Zhang, Hui Tan, Xuecai Ai, Chenhao Li, Mengru Yan, Jun Liu, Min Wu, Yeyu Feng, Defen Liu, Shaogang Han, Heyou |
| description | A sensitive electrochemiluminescence (ECL) aptasensor based on 3D graphene is described for thrombin detection utilizing nitrogen-doped graphene quantum dots doped silica (NGQDs@SiO
2
) nanocomposite labeling as an enhancer. Ru(bpy)
3
2+
-Pt nanoparticles (Ru-PtNPs) with an ECL signal were dropped onto a 3D graphene-modified electrode, and then, a complementary thrombin aptamer (cDNA) was conjugated to the Ru-PtNPs. Finally, NGQDs@SiO
2
, coupled with the thrombin aptamer as the booster, was attached to the electrode surface by using hybridization with the cDNA, and the ECL intensity was efficiently enhanced. The target thrombin induced the NGQDs@SiO
2
nanocomposites labeled with the thrombin (TB) aptamer to be extracted because of their strong binding with thrombin. The immobilization amount of NGQDs@SiO
2
on the modified electrode decreased. Thus, the presence of thrombin could result in a decrease in the ECL signal. On the basis of the signal amplification factors, the fabricated ECL aptasensor demonstrated excellent properties with concentrations varying from 2.0 pM to 50 nM and a detection limit of 23.1 fM. |
| doi_str_mv | 10.1007/s10008-019-04352-z |
| format | Article |
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2
) nanocomposite labeling as an enhancer. Ru(bpy)
3
2+
-Pt nanoparticles (Ru-PtNPs) with an ECL signal were dropped onto a 3D graphene-modified electrode, and then, a complementary thrombin aptamer (cDNA) was conjugated to the Ru-PtNPs. Finally, NGQDs@SiO
2
, coupled with the thrombin aptamer as the booster, was attached to the electrode surface by using hybridization with the cDNA, and the ECL intensity was efficiently enhanced. The target thrombin induced the NGQDs@SiO
2
nanocomposites labeled with the thrombin (TB) aptamer to be extracted because of their strong binding with thrombin. The immobilization amount of NGQDs@SiO
2
on the modified electrode decreased. Thus, the presence of thrombin could result in a decrease in the ECL signal. On the basis of the signal amplification factors, the fabricated ECL aptasensor demonstrated excellent properties with concentrations varying from 2.0 pM to 50 nM and a detection limit of 23.1 fM.</description><identifier>ISSN: 1432-8488</identifier><identifier>EISSN: 1433-0768</identifier><identifier>DOI: 10.1007/s10008-019-04352-z</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Condensed Matter Physics ; Electrochemiluminescence ; Electrochemistry ; Electrodes ; Electrons ; Energy Storage ; Graphene ; Nanocomposites ; Nanoparticles ; Original Paper ; Physical Chemistry ; Quantum dots ; Ruthenium ; Silicon dioxide ; Thrombin</subject><ispartof>Journal of solid state electrochemistry, 2019-08, Vol.23 (8), p.2579-2588</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-a9223f2b0e40a41b1029fa5c99258d65ba29172f27309e63c03f46f5098822d63</citedby><cites>FETCH-LOGICAL-c356t-a9223f2b0e40a41b1029fa5c99258d65ba29172f27309e63c03f46f5098822d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10008-019-04352-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10008-019-04352-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Du, Fangkai</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Tan, Xuecai</creatorcontrib><creatorcontrib>Ai, Chenhao</creatorcontrib><creatorcontrib>Li, Mengru</creatorcontrib><creatorcontrib>Yan, Jun</creatorcontrib><creatorcontrib>Liu, Min</creatorcontrib><creatorcontrib>Wu, Yeyu</creatorcontrib><creatorcontrib>Feng, Defen</creatorcontrib><creatorcontrib>Liu, Shaogang</creatorcontrib><creatorcontrib>Han, Heyou</creatorcontrib><title>Nitrogen-doped graphene quantum dots doped silica nanoparticles as enhancers for electrochemiluminescence thrombin aptasensors based on 3D graphene</title><title>Journal of solid state electrochemistry</title><addtitle>J Solid State Electrochem</addtitle><description>A sensitive electrochemiluminescence (ECL) aptasensor based on 3D graphene is described for thrombin detection utilizing nitrogen-doped graphene quantum dots doped silica (NGQDs@SiO
2
) nanocomposite labeling as an enhancer. Ru(bpy)
3
2+
-Pt nanoparticles (Ru-PtNPs) with an ECL signal were dropped onto a 3D graphene-modified electrode, and then, a complementary thrombin aptamer (cDNA) was conjugated to the Ru-PtNPs. Finally, NGQDs@SiO
2
, coupled with the thrombin aptamer as the booster, was attached to the electrode surface by using hybridization with the cDNA, and the ECL intensity was efficiently enhanced. The target thrombin induced the NGQDs@SiO
2
nanocomposites labeled with the thrombin (TB) aptamer to be extracted because of their strong binding with thrombin. The immobilization amount of NGQDs@SiO
2
on the modified electrode decreased. Thus, the presence of thrombin could result in a decrease in the ECL signal. On the basis of the signal amplification factors, the fabricated ECL aptasensor demonstrated excellent properties with concentrations varying from 2.0 pM to 50 nM and a detection limit of 23.1 fM.</description><subject>Analytical Chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Condensed Matter Physics</subject><subject>Electrochemiluminescence</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Electrons</subject><subject>Energy Storage</subject><subject>Graphene</subject><subject>Nanocomposites</subject><subject>Nanoparticles</subject><subject>Original Paper</subject><subject>Physical Chemistry</subject><subject>Quantum dots</subject><subject>Ruthenium</subject><subject>Silicon dioxide</subject><subject>Thrombin</subject><issn>1432-8488</issn><issn>1433-0768</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9ULtOwzAUjRBIlMIPMFliNlzbeY6oPKUKFpgtx7lpUiV2aicD_Q1-GJcg2FjuPdJ5SSeKLhlcM4DsxocLOQVWUIhFwun-KFqwWAgKWZoff2NO8zjPT6Mz77cALEsZLKLPl3Z0doOGVnbAimycGho0SHaTMuPUk8qOnsycb7tWK2KUsYNyY6s79ER5gqZRRqPzpLaOYIc6ROoG-7ab-tag1xhoMjbO9mVriBpG5dF4GxxlQBWxhoi73-7z6KRWnceLn7-M3h_u31ZPdP36-Ly6XVMtknSkquBc1LwEjEHFrGTAi1oluih4kldpUipesIzXPBNQYCo0iDpO6wSKPOe8SsUyuppzB2d3E_pRbu3kTKiUnGeQZCyFg4rPKu2s9w5rObi2V-5DMpCH8eU8vgzjy-_x5T6YxGzyQWw26P6i_3F9AS1rirs</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Du, Fangkai</creator><creator>Zhang, Hui</creator><creator>Tan, Xuecai</creator><creator>Ai, Chenhao</creator><creator>Li, Mengru</creator><creator>Yan, Jun</creator><creator>Liu, Min</creator><creator>Wu, Yeyu</creator><creator>Feng, Defen</creator><creator>Liu, Shaogang</creator><creator>Han, Heyou</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20190801</creationdate><title>Nitrogen-doped graphene quantum dots doped silica nanoparticles as enhancers for electrochemiluminescence thrombin aptasensors based on 3D graphene</title><author>Du, Fangkai ; Zhang, Hui ; Tan, Xuecai ; Ai, Chenhao ; Li, Mengru ; Yan, Jun ; Liu, Min ; Wu, Yeyu ; Feng, Defen ; Liu, Shaogang ; Han, Heyou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-a9223f2b0e40a41b1029fa5c99258d65ba29172f27309e63c03f46f5098822d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analytical Chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Condensed Matter Physics</topic><topic>Electrochemiluminescence</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Electrons</topic><topic>Energy Storage</topic><topic>Graphene</topic><topic>Nanocomposites</topic><topic>Nanoparticles</topic><topic>Original Paper</topic><topic>Physical Chemistry</topic><topic>Quantum dots</topic><topic>Ruthenium</topic><topic>Silicon dioxide</topic><topic>Thrombin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Fangkai</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Tan, Xuecai</creatorcontrib><creatorcontrib>Ai, Chenhao</creatorcontrib><creatorcontrib>Li, Mengru</creatorcontrib><creatorcontrib>Yan, Jun</creatorcontrib><creatorcontrib>Liu, Min</creatorcontrib><creatorcontrib>Wu, Yeyu</creatorcontrib><creatorcontrib>Feng, Defen</creatorcontrib><creatorcontrib>Liu, Shaogang</creatorcontrib><creatorcontrib>Han, Heyou</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of solid state electrochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Fangkai</au><au>Zhang, Hui</au><au>Tan, Xuecai</au><au>Ai, Chenhao</au><au>Li, Mengru</au><au>Yan, Jun</au><au>Liu, Min</au><au>Wu, Yeyu</au><au>Feng, Defen</au><au>Liu, Shaogang</au><au>Han, Heyou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitrogen-doped graphene quantum dots doped silica nanoparticles as enhancers for electrochemiluminescence thrombin aptasensors based on 3D graphene</atitle><jtitle>Journal of solid state electrochemistry</jtitle><stitle>J Solid State Electrochem</stitle><date>2019-08-01</date><risdate>2019</risdate><volume>23</volume><issue>8</issue><spage>2579</spage><epage>2588</epage><pages>2579-2588</pages><issn>1432-8488</issn><eissn>1433-0768</eissn><abstract>A sensitive electrochemiluminescence (ECL) aptasensor based on 3D graphene is described for thrombin detection utilizing nitrogen-doped graphene quantum dots doped silica (NGQDs@SiO
2
) nanocomposite labeling as an enhancer. Ru(bpy)
3
2+
-Pt nanoparticles (Ru-PtNPs) with an ECL signal were dropped onto a 3D graphene-modified electrode, and then, a complementary thrombin aptamer (cDNA) was conjugated to the Ru-PtNPs. Finally, NGQDs@SiO
2
, coupled with the thrombin aptamer as the booster, was attached to the electrode surface by using hybridization with the cDNA, and the ECL intensity was efficiently enhanced. The target thrombin induced the NGQDs@SiO
2
nanocomposites labeled with the thrombin (TB) aptamer to be extracted because of their strong binding with thrombin. The immobilization amount of NGQDs@SiO
2
on the modified electrode decreased. Thus, the presence of thrombin could result in a decrease in the ECL signal. On the basis of the signal amplification factors, the fabricated ECL aptasensor demonstrated excellent properties with concentrations varying from 2.0 pM to 50 nM and a detection limit of 23.1 fM.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s10008-019-04352-z</doi><tpages>10</tpages></addata></record> |
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| subjects | Analytical Chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Condensed Matter Physics Electrochemiluminescence Electrochemistry Electrodes Electrons Energy Storage Graphene Nanocomposites Nanoparticles Original Paper Physical Chemistry Quantum dots Ruthenium Silicon dioxide Thrombin |
| title | Nitrogen-doped graphene quantum dots doped silica nanoparticles as enhancers for electrochemiluminescence thrombin aptasensors based on 3D graphene |
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