Ethanol bioelectrooxidation in a robust poly(methylene green-pyrrole)- mediated enzymatic biofuel cell
We prepared robust bioanodes by immobilizing the alcohol dehydrogenase (ADH) enzyme onto a one-pot electropolymerized film consisting of methylene green and pyrrole. The enzymatic film containing immobilized ADH furnished power densities as high as 183±10μWcm−2; addition of carbon nanotubes (MWCNTs)...
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| Veröffentlicht in: | Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2019-07, Vol.844, p.43-48 |
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| container_title | Journal of electroanalytical chemistry (Lausanne, Switzerland) |
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| creator | Bonfin, Carolina Souza Franco, Jefferson Honorio de Andrade, Adalgisa R. |
| description | We prepared robust bioanodes by immobilizing the alcohol dehydrogenase (ADH) enzyme onto a one-pot electropolymerized film consisting of methylene green and pyrrole. The enzymatic film containing immobilized ADH furnished power densities as high as 183±10μWcm−2; addition of carbon nanotubes (MWCNTs) increased the bioanode power density to 275±12μWcm−2. The bioanode was very stable, with a mean decay of 36% of its initial power density after 20weeks of storage. Long-term ethanol electrolysis (12h) followed by HPLC-UV/RID confirmed that the system was highly selective and generated a two-electron product. MWNCT introduction provided five times higher acetaldehyde production. Compared to literature data, results were promising and showed that the system can be used to construct biofuel cells. In addition, the film obtained by simultaneous methylene green and pyrrole electropolymerization afforded interesting results and can be applied in other systems, such as biosensors.
[Display omitted]
•One-pot electropolymerized methylene green and pyrrole can catalyze ethanol oxidation.•Poly-(MG-PYR)ADH film bioanode has good stability.•HPLC confirms two electrons per ethanol molecule after 12h of electrolysis.•MWCNT addition to the bioanode increases acetaldehyde production. |
| doi_str_mv | 10.1016/j.jelechem.2019.04.075 |
| format | Article |
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[Display omitted]
•One-pot electropolymerized methylene green and pyrrole can catalyze ethanol oxidation.•Poly-(MG-PYR)ADH film bioanode has good stability.•HPLC confirms two electrons per ethanol molecule after 12h of electrolysis.•MWCNT addition to the bioanode increases acetaldehyde production.</description><identifier>ISSN: 1572-6657</identifier><identifier>EISSN: 1873-2569</identifier><identifier>DOI: 10.1016/j.jelechem.2019.04.075</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetaldehyde ; Alcohol dehydrogenase ; Biochemical fuel cells ; Biodiesel fuels ; Biomass ; Biosensors ; Electrolysis ; Electrolytic cells ; Enzymatic biofuel cell ; Ethanol ; Fuels ; Methylene ; Multi wall carbon nanotubes ; Poly(methylene green) ; Poly(pyrrole) ; Polymerization ; Simultaneous electropolymerization</subject><ispartof>Journal of electroanalytical chemistry (Lausanne, Switzerland), 2019-07, Vol.844, p.43-48</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright Elsevier Science Ltd. Jul 1, 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-ebc8e4b9bfe8ca628f2117b0734220fc1d06cfb267b35af35417a576262e16f63</citedby><cites>FETCH-LOGICAL-c454t-ebc8e4b9bfe8ca628f2117b0734220fc1d06cfb267b35af35417a576262e16f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S157266571930339X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Bonfin, Carolina Souza</creatorcontrib><creatorcontrib>Franco, Jefferson Honorio</creatorcontrib><creatorcontrib>de Andrade, Adalgisa R.</creatorcontrib><title>Ethanol bioelectrooxidation in a robust poly(methylene green-pyrrole)- mediated enzymatic biofuel cell</title><title>Journal of electroanalytical chemistry (Lausanne, Switzerland)</title><description>We prepared robust bioanodes by immobilizing the alcohol dehydrogenase (ADH) enzyme onto a one-pot electropolymerized film consisting of methylene green and pyrrole. The enzymatic film containing immobilized ADH furnished power densities as high as 183±10μWcm−2; addition of carbon nanotubes (MWCNTs) increased the bioanode power density to 275±12μWcm−2. The bioanode was very stable, with a mean decay of 36% of its initial power density after 20weeks of storage. Long-term ethanol electrolysis (12h) followed by HPLC-UV/RID confirmed that the system was highly selective and generated a two-electron product. MWNCT introduction provided five times higher acetaldehyde production. Compared to literature data, results were promising and showed that the system can be used to construct biofuel cells. In addition, the film obtained by simultaneous methylene green and pyrrole electropolymerization afforded interesting results and can be applied in other systems, such as biosensors.
[Display omitted]
•One-pot electropolymerized methylene green and pyrrole can catalyze ethanol oxidation.•Poly-(MG-PYR)ADH film bioanode has good stability.•HPLC confirms two electrons per ethanol molecule after 12h of electrolysis.•MWCNT addition to the bioanode increases acetaldehyde production.</description><subject>Acetaldehyde</subject><subject>Alcohol dehydrogenase</subject><subject>Biochemical fuel cells</subject><subject>Biodiesel fuels</subject><subject>Biomass</subject><subject>Biosensors</subject><subject>Electrolysis</subject><subject>Electrolytic cells</subject><subject>Enzymatic biofuel cell</subject><subject>Ethanol</subject><subject>Fuels</subject><subject>Methylene</subject><subject>Multi wall carbon nanotubes</subject><subject>Poly(methylene green)</subject><subject>Poly(pyrrole)</subject><subject>Polymerization</subject><subject>Simultaneous electropolymerization</subject><issn>1572-6657</issn><issn>1873-2569</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LxDAQhosouK7-BQl40UNrkjZJe1OW9QMWvOg5pOnETUmbNe2K9debsnr2NHN432eYJ0kuCc4IJvy2zVpwoLfQZRSTKsNFhgU7ShakFHlKGa-O484ETTln4jQ5G4YWY1qWhC4Ssx63qvcO1dbPlDF4_2UbNVrfI9sjhYKv98OIdt5N1x2M28lBD-g9APTpbgrBO7hJUQeNVSM0CPrvqYt1PRPNHhzS4Nx5cmKUG-Didy6Tt4f16-op3bw8Pq_uN6kuWDGmUOsSirqqDZRacVoaSoioscgLSrHRpMFcm5pyUedMmZwVRCgmOOUUCDc8XyZXB-4u-I89DKNs_T708aSklFcVK3NMY4ofUjr4YQhg5C7YToVJEixnp7KVf07l7FTiQkansXh3KEL84dNCkIO20Ov4fIjuZOPtf4gfKsyE7w</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Bonfin, Carolina Souza</creator><creator>Franco, Jefferson Honorio</creator><creator>de Andrade, Adalgisa R.</creator><general>Elsevier B.V</general><general>Elsevier Science Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20190701</creationdate><title>Ethanol bioelectrooxidation in a robust poly(methylene green-pyrrole)- mediated enzymatic biofuel cell</title><author>Bonfin, Carolina Souza ; Franco, Jefferson Honorio ; de Andrade, Adalgisa R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-ebc8e4b9bfe8ca628f2117b0734220fc1d06cfb267b35af35417a576262e16f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetaldehyde</topic><topic>Alcohol dehydrogenase</topic><topic>Biochemical fuel cells</topic><topic>Biodiesel fuels</topic><topic>Biomass</topic><topic>Biosensors</topic><topic>Electrolysis</topic><topic>Electrolytic cells</topic><topic>Enzymatic biofuel cell</topic><topic>Ethanol</topic><topic>Fuels</topic><topic>Methylene</topic><topic>Multi wall carbon nanotubes</topic><topic>Poly(methylene green)</topic><topic>Poly(pyrrole)</topic><topic>Polymerization</topic><topic>Simultaneous electropolymerization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bonfin, Carolina Souza</creatorcontrib><creatorcontrib>Franco, Jefferson Honorio</creatorcontrib><creatorcontrib>de Andrade, Adalgisa R.</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Journal of electroanalytical chemistry (Lausanne, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bonfin, Carolina Souza</au><au>Franco, Jefferson Honorio</au><au>de Andrade, Adalgisa R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ethanol bioelectrooxidation in a robust poly(methylene green-pyrrole)- mediated enzymatic biofuel cell</atitle><jtitle>Journal of electroanalytical chemistry (Lausanne, Switzerland)</jtitle><date>2019-07-01</date><risdate>2019</risdate><volume>844</volume><spage>43</spage><epage>48</epage><pages>43-48</pages><issn>1572-6657</issn><eissn>1873-2569</eissn><abstract>We prepared robust bioanodes by immobilizing the alcohol dehydrogenase (ADH) enzyme onto a one-pot electropolymerized film consisting of methylene green and pyrrole. The enzymatic film containing immobilized ADH furnished power densities as high as 183±10μWcm−2; addition of carbon nanotubes (MWCNTs) increased the bioanode power density to 275±12μWcm−2. The bioanode was very stable, with a mean decay of 36% of its initial power density after 20weeks of storage. Long-term ethanol electrolysis (12h) followed by HPLC-UV/RID confirmed that the system was highly selective and generated a two-electron product. MWNCT introduction provided five times higher acetaldehyde production. Compared to literature data, results were promising and showed that the system can be used to construct biofuel cells. In addition, the film obtained by simultaneous methylene green and pyrrole electropolymerization afforded interesting results and can be applied in other systems, such as biosensors.
[Display omitted]
•One-pot electropolymerized methylene green and pyrrole can catalyze ethanol oxidation.•Poly-(MG-PYR)ADH film bioanode has good stability.•HPLC confirms two electrons per ethanol molecule after 12h of electrolysis.•MWCNT addition to the bioanode increases acetaldehyde production.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.jelechem.2019.04.075</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1572-6657 |
| ispartof | Journal of electroanalytical chemistry (Lausanne, Switzerland), 2019-07, Vol.844, p.43-48 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Acetaldehyde Alcohol dehydrogenase Biochemical fuel cells Biodiesel fuels Biomass Biosensors Electrolysis Electrolytic cells Enzymatic biofuel cell Ethanol Fuels Methylene Multi wall carbon nanotubes Poly(methylene green) Poly(pyrrole) Polymerization Simultaneous electropolymerization |
| title | Ethanol bioelectrooxidation in a robust poly(methylene green-pyrrole)- mediated enzymatic biofuel cell |
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