Molecular Cloning and Functional Characterization of Mouse Innate Immune Sensor RIG-I
Innate immunity functions as the first line against infection, which is mediated by series of innate immune receptors. RIG-I like receptors (RLR) recognize the cytosolic nucleic acids mainly from viruses, trigger the type I interferon (IFN) production, and thus play essential roles in the anti-viral...
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| Veröffentlicht in: | Cytology and genetics 2019-07, Vol.53 (4), p.325-329 |
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| creator | Tangjie Zhang Sarkar, Saumendra N. Zhu, Jianzhong |
| description | Innate immunity functions as the first line against infection, which is mediated by series of innate immune receptors. RIG-I like receptors (RLR) recognize the cytosolic nucleic acids mainly from viruses, trigger the type I interferon (IFN) production, and thus play essential roles in the anti-viral immunity. Here we cloned the mouse RIG-I (mRIG-I) cDNA into the pENTR vector using the TOPO cloning technology, and transferred the FLAG tagged mRIG-I gene from pENTR vector to destination pLenti viral vector. The expression of mRIG-I in transfected cells was detected by immunoblotting using anti-FLAG and anti-RIG-I antibodies. The transfected mRIG-I was active capable of inducing downstream IFN stimulated gene (ISG) gene transcription as well as protein expression. |
| doi_str_mv | 10.3103/S0095452719040121 |
| format | Article |
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RIG-I like receptors (RLR) recognize the cytosolic nucleic acids mainly from viruses, trigger the type I interferon (IFN) production, and thus play essential roles in the anti-viral immunity. Here we cloned the mouse RIG-I (mRIG-I) cDNA into the pENTR vector using the TOPO cloning technology, and transferred the FLAG tagged mRIG-I gene from pENTR vector to destination pLenti viral vector. The expression of mRIG-I in transfected cells was detected by immunoblotting using anti-FLAG and anti-RIG-I antibodies. The transfected mRIG-I was active capable of inducing downstream IFN stimulated gene (ISG) gene transcription as well as protein expression.</description><identifier>ISSN: 0095-4527</identifier><identifier>EISSN: 1934-9440</identifier><identifier>DOI: 10.3103/S0095452719040121</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Antiviral agents ; Biomedical and Life Sciences ; Biomedicine ; Cloning ; Cloning vectors ; Human Genetics ; Immunoblotting ; Innate immunity ; Interferon ; Transcription</subject><ispartof>Cytology and genetics, 2019-07, Vol.53 (4), p.325-329</ispartof><rights>Allerton Press, Inc. 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c268t-6bff8dd70f85c9edea8078fbe734f1b28616bdd49dacb1742589821255138b5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3103/S0095452719040121$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3103/S0095452719040121$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids></links><search><creatorcontrib>Tangjie Zhang</creatorcontrib><creatorcontrib>Sarkar, Saumendra N.</creatorcontrib><creatorcontrib>Zhu, Jianzhong</creatorcontrib><title>Molecular Cloning and Functional Characterization of Mouse Innate Immune Sensor RIG-I</title><title>Cytology and genetics</title><addtitle>Cytol. Genet</addtitle><description>Innate immunity functions as the first line against infection, which is mediated by series of innate immune receptors. RIG-I like receptors (RLR) recognize the cytosolic nucleic acids mainly from viruses, trigger the type I interferon (IFN) production, and thus play essential roles in the anti-viral immunity. Here we cloned the mouse RIG-I (mRIG-I) cDNA into the pENTR vector using the TOPO cloning technology, and transferred the FLAG tagged mRIG-I gene from pENTR vector to destination pLenti viral vector. The expression of mRIG-I in transfected cells was detected by immunoblotting using anti-FLAG and anti-RIG-I antibodies. The transfected mRIG-I was active capable of inducing downstream IFN stimulated gene (ISG) gene transcription as well as protein expression.</description><subject>Antiviral agents</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cloning</subject><subject>Cloning vectors</subject><subject>Human Genetics</subject><subject>Immunoblotting</subject><subject>Innate immunity</subject><subject>Interferon</subject><subject>Transcription</subject><issn>0095-4527</issn><issn>1934-9440</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kE1Lw0AYhBdRsFZ_gLcFz9H9THaPEmwNtAjWnsNmP2pKult3k4P-ehMqeBBPA-_MM7wMALcY3VOM6MMGIckZJwWWiCFM8BmYYUlZJhlD52A22dnkX4KrlPYI8ZxQOgPbdeisHjoVYdkF3_odVN7AxeB13wavOli-q6h0b2P7paYTDA6uw5AsrLxX_SiHw-At3FifQoSv1TKrrsGFU12yNz86B9vF01v5nK1ellX5uMo0yUWf5Y1zwpgCOcG1tMYqgQrhGltQ5nBDRI7zxhgmjdINLhjhQgqCCeeYioZbOgd3p95jDB-DTX29D0Mcv041IbkcAYzyMYVPKR1DStG6-hjbg4qfNUb1tF79Z72RIScmjVm_s_G3-X_oG76AcEA</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Tangjie Zhang</creator><creator>Sarkar, Saumendra N.</creator><creator>Zhu, Jianzhong</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20190701</creationdate><title>Molecular Cloning and Functional Characterization of Mouse Innate Immune Sensor RIG-I</title><author>Tangjie Zhang ; Sarkar, Saumendra N. ; Zhu, Jianzhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-6bff8dd70f85c9edea8078fbe734f1b28616bdd49dacb1742589821255138b5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antiviral agents</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cloning</topic><topic>Cloning vectors</topic><topic>Human Genetics</topic><topic>Immunoblotting</topic><topic>Innate immunity</topic><topic>Interferon</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tangjie Zhang</creatorcontrib><creatorcontrib>Sarkar, Saumendra N.</creatorcontrib><creatorcontrib>Zhu, Jianzhong</creatorcontrib><collection>CrossRef</collection><jtitle>Cytology and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tangjie Zhang</au><au>Sarkar, Saumendra N.</au><au>Zhu, Jianzhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Cloning and Functional Characterization of Mouse Innate Immune Sensor RIG-I</atitle><jtitle>Cytology and genetics</jtitle><stitle>Cytol. 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The transfected mRIG-I was active capable of inducing downstream IFN stimulated gene (ISG) gene transcription as well as protein expression.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.3103/S0095452719040121</doi><tpages>5</tpages></addata></record> |
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| ispartof | Cytology and genetics, 2019-07, Vol.53 (4), p.325-329 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Antiviral agents Biomedical and Life Sciences Biomedicine Cloning Cloning vectors Human Genetics Immunoblotting Innate immunity Interferon Transcription |
| title | Molecular Cloning and Functional Characterization of Mouse Innate Immune Sensor RIG-I |
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