Isolation and characterization of polymorphic microsatellite markers in lesser kestrel (Falco naumanni) and cross-amplification in common kestrel (Falco tinnunculus)
Lesser kestrel, Falco naumanni, is a colonial and migratory species breeding in part of the Mediterranean Basin and part of central Asia and north-east of China and Mongolia. This species is catalogued in IUCN red list category as vulnerable. Twenty microsatellite loci were selected from libraries e...
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| Veröffentlicht in: | Conservation genetics 2009-10, Vol.10 (5), p.1357-1360 |
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| creator | Padilla, J. A Parejo, J. C Salazar, J Martínez-Trancón, M Rabasco, A Sansinforiano, E Quesada, A |
| description | Lesser kestrel, Falco naumanni, is a colonial and migratory species breeding in part of the Mediterranean Basin and part of central Asia and north-east of China and Mongolia. This species is catalogued in IUCN red list category as vulnerable. Twenty microsatellite loci were selected from libraries enriched in AC or AG tandem repeats and specific PCR were devised from their flanking sequences. Most microsatellites (14) were found polymorphic among 30 individuals of F. naumanni representing 20 reproduction areas of the species in the region of Extremadura, Spain. Polymorphisms were detected by size variation of the amplified loci, which allele number and observed heterozygosity ranged from 3 to 20 and from 0.300 to 0.933, respectively. Cross-species amplification showed that 13 of selected loci were also found polymorphic in common kestrel species (Falco tinnunculus). Novel polymorphic microsatellites will serve to conservation studies in lesser kestrel. |
| doi_str_mv | 10.1007/s10592-008-9711-1 |
| format | Article |
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A ; Parejo, J. C ; Salazar, J ; Martínez-Trancón, M ; Rabasco, A ; Sansinforiano, E ; Quesada, A</creator><creatorcontrib>Padilla, J. A ; Parejo, J. C ; Salazar, J ; Martínez-Trancón, M ; Rabasco, A ; Sansinforiano, E ; Quesada, A</creatorcontrib><description>Lesser kestrel, Falco naumanni, is a colonial and migratory species breeding in part of the Mediterranean Basin and part of central Asia and north-east of China and Mongolia. This species is catalogued in IUCN red list category as vulnerable. Twenty microsatellite loci were selected from libraries enriched in AC or AG tandem repeats and specific PCR were devised from their flanking sequences. Most microsatellites (14) were found polymorphic among 30 individuals of F. naumanni representing 20 reproduction areas of the species in the region of Extremadura, Spain. Polymorphisms were detected by size variation of the amplified loci, which allele number and observed heterozygosity ranged from 3 to 20 and from 0.300 to 0.933, respectively. Cross-species amplification showed that 13 of selected loci were also found polymorphic in common kestrel species (Falco tinnunculus). 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C</creatorcontrib><creatorcontrib>Salazar, J</creatorcontrib><creatorcontrib>Martínez-Trancón, M</creatorcontrib><creatorcontrib>Rabasco, A</creatorcontrib><creatorcontrib>Sansinforiano, E</creatorcontrib><creatorcontrib>Quesada, A</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Conservation genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Padilla, J. A</au><au>Parejo, J. C</au><au>Salazar, J</au><au>Martínez-Trancón, M</au><au>Rabasco, A</au><au>Sansinforiano, E</au><au>Quesada, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of polymorphic microsatellite markers in lesser kestrel (Falco naumanni) and cross-amplification in common kestrel (Falco tinnunculus)</atitle><jtitle>Conservation genetics</jtitle><stitle>Conserv Genet</stitle><date>2009-10-01</date><risdate>2009</risdate><volume>10</volume><issue>5</issue><spage>1357</spage><epage>1360</epage><pages>1357-1360</pages><issn>1566-0621</issn><eissn>1572-9737</eissn><abstract>Lesser kestrel, Falco naumanni, is a colonial and migratory species breeding in part of the Mediterranean Basin and part of central Asia and north-east of China and Mongolia. This species is catalogued in IUCN red list category as vulnerable. 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| subjects | Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Birds Conservation Biology/Ecology Ecology Evolutionary Biology Genetic markers Life Sciences Migratory species Plant Genetics and Genomics Polymorphism Population genetics Short Communication |
| title | Isolation and characterization of polymorphic microsatellite markers in lesser kestrel (Falco naumanni) and cross-amplification in common kestrel (Falco tinnunculus) |
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