Effect of alkali on konjac glucomannan film and its application on wound healing
Konjac glucomannan (KGM) is widely used in the food industry because of its biocompatibility, nontoxicity, and favorable film-forming ability. However, KGM has been seldom applied to biomaterials for tissue regeneration. In this paper, we investigate the effects of Ca(OH)₂on KGM film and evaluate it...
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| Veröffentlicht in: | Cellulose (London) 2015-02, Vol.22 (1), p.737-747 |
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| creator | Huang, Yi-Cheng Yang, Chi-Yu Chu, Hao-Wen Wu, Wen-Ching Tsai, Jenn-Shou |
| description | Konjac glucomannan (KGM) is widely used in the food industry because of its biocompatibility, nontoxicity, and favorable film-forming ability. However, KGM has been seldom applied to biomaterials for tissue regeneration. In this paper, we investigate the effects of Ca(OH)₂on KGM film and evaluate its potency as a wound dressing. We successfully prepared Ca(OH)₂-treated KGM film. High Ca(OH)₂concentrations created rough film surfaces. These rough surfaces resulted from the deposition of nanosized or microsized CaCO₃₍ₛ₎. Compared with commercially available 3M hydrocolloid dressings, dressings with KGM exhibited a more favorable degree of swelling and water vapor transmission rate. The film with a Ca(OH)₂/KGM weight ratio of 2.5 % exhibited the optimal tensile strength and elongation. The results of an in vitro MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] cell viability assay revealed the favorable biocompatibility of KGM films with L929 cells. For antibacterial evaluation, tetracycline-loaded KGM film exhibited strong inhibitory effects against Staphylococcus aureus with a 2.5-cm inhibition zone. In vivo animal studies have indicated that KGM films effectively promote the contractility of wounds, especially at the early healing stage. Histological examinations have also demonstrated that using KGM films to treat wounds enables considerably advanced granulation tissue and epithelial coverage to develop by the 7th and 14th days of treatment. In summary, Ca(OH)₂-treated KGM film could be considered as a promising, novel, biocompatible wound dressing. |
| doi_str_mv | 10.1007/s10570-014-0512-z |
| format | Article |
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However, KGM has been seldom applied to biomaterials for tissue regeneration. In this paper, we investigate the effects of Ca(OH)₂on KGM film and evaluate its potency as a wound dressing. We successfully prepared Ca(OH)₂-treated KGM film. High Ca(OH)₂concentrations created rough film surfaces. These rough surfaces resulted from the deposition of nanosized or microsized CaCO₃₍ₛ₎. Compared with commercially available 3M hydrocolloid dressings, dressings with KGM exhibited a more favorable degree of swelling and water vapor transmission rate. The film with a Ca(OH)₂/KGM weight ratio of 2.5 % exhibited the optimal tensile strength and elongation. The results of an in vitro MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] cell viability assay revealed the favorable biocompatibility of KGM films with L929 cells. For antibacterial evaluation, tetracycline-loaded KGM film exhibited strong inhibitory effects against Staphylococcus aureus with a 2.5-cm inhibition zone. In vivo animal studies have indicated that KGM films effectively promote the contractility of wounds, especially at the early healing stage. Histological examinations have also demonstrated that using KGM films to treat wounds enables considerably advanced granulation tissue and epithelial coverage to develop by the 7th and 14th days of treatment. In summary, Ca(OH)₂-treated KGM film could be considered as a promising, novel, biocompatible wound dressing.</description><identifier>ISSN: 0969-0239</identifier><identifier>EISSN: 1572-882X</identifier><identifier>DOI: 10.1007/s10570-014-0512-z</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>animals ; Biocompatibility ; biocompatible materials ; Biomedical materials ; Bioorganic Chemistry ; Calcium carbonate ; calcium hydroxide ; cell viability ; cellulose ; Ceramics ; Chemistry ; Chemistry and Materials Science ; Composites ; Elongation ; Food processing industry ; Glass ; Granulation ; granulation tissue ; hydrocolloids ; In vivo methods and tests ; konjac mannan ; Natural Materials ; Organic Chemistry ; Original Paper ; Physical Chemistry ; Polymer Sciences ; Regeneration ; Slaked lime ; Staphylococcus aureus ; Sustainable Development ; tensile strength ; Tissue engineering ; tissue repair ; Water vapor ; Wound healing</subject><ispartof>Cellulose (London), 2015-02, Vol.22 (1), p.737-747</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><rights>Cellulose is a copyright of Springer, (2014). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-a2590f01a51815af521783badf42d32b8b967b77d055e1745fef5bda85069a043</citedby><cites>FETCH-LOGICAL-c410t-a2590f01a51815af521783badf42d32b8b967b77d055e1745fef5bda85069a043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10570-014-0512-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10570-014-0512-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Huang, Yi-Cheng</creatorcontrib><creatorcontrib>Yang, Chi-Yu</creatorcontrib><creatorcontrib>Chu, Hao-Wen</creatorcontrib><creatorcontrib>Wu, Wen-Ching</creatorcontrib><creatorcontrib>Tsai, Jenn-Shou</creatorcontrib><title>Effect of alkali on konjac glucomannan film and its application on wound healing</title><title>Cellulose (London)</title><addtitle>Cellulose</addtitle><description>Konjac glucomannan (KGM) is widely used in the food industry because of its biocompatibility, nontoxicity, and favorable film-forming ability. However, KGM has been seldom applied to biomaterials for tissue regeneration. In this paper, we investigate the effects of Ca(OH)₂on KGM film and evaluate its potency as a wound dressing. We successfully prepared Ca(OH)₂-treated KGM film. High Ca(OH)₂concentrations created rough film surfaces. These rough surfaces resulted from the deposition of nanosized or microsized CaCO₃₍ₛ₎. Compared with commercially available 3M hydrocolloid dressings, dressings with KGM exhibited a more favorable degree of swelling and water vapor transmission rate. The film with a Ca(OH)₂/KGM weight ratio of 2.5 % exhibited the optimal tensile strength and elongation. The results of an in vitro MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] cell viability assay revealed the favorable biocompatibility of KGM films with L929 cells. For antibacterial evaluation, tetracycline-loaded KGM film exhibited strong inhibitory effects against Staphylococcus aureus with a 2.5-cm inhibition zone. In vivo animal studies have indicated that KGM films effectively promote the contractility of wounds, especially at the early healing stage. Histological examinations have also demonstrated that using KGM films to treat wounds enables considerably advanced granulation tissue and epithelial coverage to develop by the 7th and 14th days of treatment. In summary, Ca(OH)₂-treated KGM film could be considered as a promising, novel, biocompatible wound dressing.</description><subject>animals</subject><subject>Biocompatibility</subject><subject>biocompatible materials</subject><subject>Biomedical materials</subject><subject>Bioorganic Chemistry</subject><subject>Calcium carbonate</subject><subject>calcium hydroxide</subject><subject>cell viability</subject><subject>cellulose</subject><subject>Ceramics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Elongation</subject><subject>Food processing industry</subject><subject>Glass</subject><subject>Granulation</subject><subject>granulation tissue</subject><subject>hydrocolloids</subject><subject>In vivo methods and tests</subject><subject>konjac mannan</subject><subject>Natural Materials</subject><subject>Organic Chemistry</subject><subject>Original Paper</subject><subject>Physical Chemistry</subject><subject>Polymer Sciences</subject><subject>Regeneration</subject><subject>Slaked lime</subject><subject>Staphylococcus aureus</subject><subject>Sustainable Development</subject><subject>tensile strength</subject><subject>Tissue engineering</subject><subject>tissue repair</subject><subject>Water vapor</subject><subject>Wound healing</subject><issn>0969-0239</issn><issn>1572-882X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kE1LAzEQhoMoWKs_wJMBz6sz2U2zOUqpH1BQ0IK3MPuRuu02qZstYn-9KSt4EwbmMM_zDryMXSLcIIC6DQhSQQKYJSBRJPsjNkKpRJLn4v2YjUBPdAIi1afsLIQVAGglcMReZtbWZc-95dSuqW24d3zt3YpKvmx3pd-Qc-S4bdoNJ1fxpg-cttu2KalvIhvny-_i4aOOtluesxNLbagvfveYLe5nb9PHZP788DS9mydlhtAnJKQGC0gSc5RkpUCVpwVVNhNVKoq80BNVKFWBlDWqTNrayqKiXMJEE2TpmF0PudvOf-7q0JuV33UuvjQiZmuhEXWkcKDKzofQ1dZsu2ZD3bdBMIfizFCcicWZQ3FmHx0xOCGybll3f8n_SVeDZMkbWnZNMItXASghQlJmOv0BQW15ag</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Huang, Yi-Cheng</creator><creator>Yang, Chi-Yu</creator><creator>Chu, Hao-Wen</creator><creator>Wu, Wen-Ching</creator><creator>Tsai, Jenn-Shou</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20150201</creationdate><title>Effect of alkali on konjac glucomannan film and its application on wound healing</title><author>Huang, Yi-Cheng ; Yang, Chi-Yu ; Chu, Hao-Wen ; Wu, Wen-Ching ; Tsai, Jenn-Shou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-a2590f01a51815af521783badf42d32b8b967b77d055e1745fef5bda85069a043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>animals</topic><topic>Biocompatibility</topic><topic>biocompatible materials</topic><topic>Biomedical materials</topic><topic>Bioorganic Chemistry</topic><topic>Calcium carbonate</topic><topic>calcium hydroxide</topic><topic>cell viability</topic><topic>cellulose</topic><topic>Ceramics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Elongation</topic><topic>Food processing industry</topic><topic>Glass</topic><topic>Granulation</topic><topic>granulation tissue</topic><topic>hydrocolloids</topic><topic>In vivo methods and tests</topic><topic>konjac mannan</topic><topic>Natural Materials</topic><topic>Organic Chemistry</topic><topic>Original Paper</topic><topic>Physical Chemistry</topic><topic>Polymer Sciences</topic><topic>Regeneration</topic><topic>Slaked lime</topic><topic>Staphylococcus aureus</topic><topic>Sustainable Development</topic><topic>tensile strength</topic><topic>Tissue engineering</topic><topic>tissue repair</topic><topic>Water vapor</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yi-Cheng</creatorcontrib><creatorcontrib>Yang, Chi-Yu</creatorcontrib><creatorcontrib>Chu, Hao-Wen</creatorcontrib><creatorcontrib>Wu, Wen-Ching</creatorcontrib><creatorcontrib>Tsai, Jenn-Shou</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Cellulose (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yi-Cheng</au><au>Yang, Chi-Yu</au><au>Chu, Hao-Wen</au><au>Wu, Wen-Ching</au><au>Tsai, Jenn-Shou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of alkali on konjac glucomannan film and its application on wound healing</atitle><jtitle>Cellulose (London)</jtitle><stitle>Cellulose</stitle><date>2015-02-01</date><risdate>2015</risdate><volume>22</volume><issue>1</issue><spage>737</spage><epage>747</epage><pages>737-747</pages><issn>0969-0239</issn><eissn>1572-882X</eissn><abstract>Konjac glucomannan (KGM) is widely used in the food industry because of its biocompatibility, nontoxicity, and favorable film-forming ability. However, KGM has been seldom applied to biomaterials for tissue regeneration. In this paper, we investigate the effects of Ca(OH)₂on KGM film and evaluate its potency as a wound dressing. We successfully prepared Ca(OH)₂-treated KGM film. High Ca(OH)₂concentrations created rough film surfaces. These rough surfaces resulted from the deposition of nanosized or microsized CaCO₃₍ₛ₎. Compared with commercially available 3M hydrocolloid dressings, dressings with KGM exhibited a more favorable degree of swelling and water vapor transmission rate. The film with a Ca(OH)₂/KGM weight ratio of 2.5 % exhibited the optimal tensile strength and elongation. The results of an in vitro MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] cell viability assay revealed the favorable biocompatibility of KGM films with L929 cells. For antibacterial evaluation, tetracycline-loaded KGM film exhibited strong inhibitory effects against Staphylococcus aureus with a 2.5-cm inhibition zone. In vivo animal studies have indicated that KGM films effectively promote the contractility of wounds, especially at the early healing stage. Histological examinations have also demonstrated that using KGM films to treat wounds enables considerably advanced granulation tissue and epithelial coverage to develop by the 7th and 14th days of treatment. In summary, Ca(OH)₂-treated KGM film could be considered as a promising, novel, biocompatible wound dressing.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><doi>10.1007/s10570-014-0512-z</doi><tpages>11</tpages></addata></record> |
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| subjects | animals Biocompatibility biocompatible materials Biomedical materials Bioorganic Chemistry Calcium carbonate calcium hydroxide cell viability cellulose Ceramics Chemistry Chemistry and Materials Science Composites Elongation Food processing industry Glass Granulation granulation tissue hydrocolloids In vivo methods and tests konjac mannan Natural Materials Organic Chemistry Original Paper Physical Chemistry Polymer Sciences Regeneration Slaked lime Staphylococcus aureus Sustainable Development tensile strength Tissue engineering tissue repair Water vapor Wound healing |
| title | Effect of alkali on konjac glucomannan film and its application on wound healing |
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