Optimization of cold-active CMCase production by psychrotrophic Sphingomonas sp. FLX-7 from the cold region of China
Organic solid waste consists of 70 % biodegradables, such as cellulose, which is unable to be degraded quickly and efficiently in the cold region of China. Psychrotrophic cellulose-degrading bacteria play an important role in cellulose degradation at low temperatures. In this study, a psychrotrophic...
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| Veröffentlicht in: | Cellulose (London) 2016-04, Vol.23 (2), p.1335-1347 |
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| creator | Li, Dapeng Feng, Lu Liu, Keran Cheng, Yi Hou, Ning Li, Chunyan |
| description | Organic solid waste consists of 70 % biodegradables, such as cellulose, which is unable to be degraded quickly and efficiently in the cold region of China. Psychrotrophic cellulose-degrading bacteria play an important role in cellulose degradation at low temperatures. In this study, a psychrotrophic cellulose-degrading FLX-7 strain with high carboxymethyl cellulase (CMCase) activity was isolated from the soil of a solid waste landfill under the environmental temperature of −10 °C. The FLX-7 strain was identified as Sphingobacterium sp. based on morphology and physiological and biochemical properties as well as 16S rRNA gene sequence analysis. To improve the cold-active CMCase production of FLX-7, fermentation conditions were optimized using response surface methodology based on a Box–Behnken design. The maximum CMCase activity (16.95 U/mL) was obtained at optimal fermentation conditions (inoculum size 2.1 %, temperature 10.4 °C, incubation time 73.5 h, and pH 7.2). The optimum pH and temperature for enzymatic reaction of cold-active CMCase were 5.0 and 25 °C, respectively. The cold-active CMCase was stable at a wide pH range of 5.0–9.0 and temperature range of 10–30 °C. The FLX-7 strain could be distributed via a psychrotrophic cellulose-degrading strain library and applied more broadly to organic solid waste composting in cold regions. |
| doi_str_mv | 10.1007/s10570-016-0859-4 |
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FLX-7 from the cold region of China</title><source>Springer Nature - Complete Springer Journals</source><creator>Li, Dapeng ; Feng, Lu ; Liu, Keran ; Cheng, Yi ; Hou, Ning ; Li, Chunyan</creator><creatorcontrib>Li, Dapeng ; Feng, Lu ; Liu, Keran ; Cheng, Yi ; Hou, Ning ; Li, Chunyan</creatorcontrib><description>Organic solid waste consists of 70 % biodegradables, such as cellulose, which is unable to be degraded quickly and efficiently in the cold region of China. Psychrotrophic cellulose-degrading bacteria play an important role in cellulose degradation at low temperatures. In this study, a psychrotrophic cellulose-degrading FLX-7 strain with high carboxymethyl cellulase (CMCase) activity was isolated from the soil of a solid waste landfill under the environmental temperature of −10 °C. The FLX-7 strain was identified as Sphingobacterium sp. based on morphology and physiological and biochemical properties as well as 16S rRNA gene sequence analysis. To improve the cold-active CMCase production of FLX-7, fermentation conditions were optimized using response surface methodology based on a Box–Behnken design. The maximum CMCase activity (16.95 U/mL) was obtained at optimal fermentation conditions (inoculum size 2.1 %, temperature 10.4 °C, incubation time 73.5 h, and pH 7.2). The optimum pH and temperature for enzymatic reaction of cold-active CMCase were 5.0 and 25 °C, respectively. The cold-active CMCase was stable at a wide pH range of 5.0–9.0 and temperature range of 10–30 °C. The FLX-7 strain could be distributed via a psychrotrophic cellulose-degrading strain library and applied more broadly to organic solid waste composting in cold regions.</description><identifier>ISSN: 0969-0239</identifier><identifier>EISSN: 1572-882X</identifier><identifier>DOI: 10.1007/s10570-016-0859-4</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>ambient temperature ; bacteria ; Bioorganic Chemistry ; Cellulase ; Cellulose ; Ceramics ; Chemistry ; Chemistry and Materials Science ; Cold ; cold zones ; Composites ; Composting ; Degradation ; endo-1,4-beta-glucanase ; enzymatic reactions ; Fermentation ; Glass ; Inoculum ; landfills ; Morphology ; Natural Materials ; nucleotide sequences ; Optimization ; Organic Chemistry ; Original Paper ; Physical Chemistry ; Polymer Sciences ; Response surface methodology ; ribosomal RNA ; sequence analysis ; soil ; solid wastes ; Sphingobacterium ; Sphingomonas ; Sustainable Development ; Waste management industry</subject><ispartof>Cellulose (London), 2016-04, Vol.23 (2), p.1335-1347</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><rights>Cellulose is a copyright of Springer, (2016). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-b9528a654256c21d5945b4c1bd62795fcefabe6bf519fb1558e22bb1cac0b58d3</citedby><cites>FETCH-LOGICAL-c377t-b9528a654256c21d5945b4c1bd62795fcefabe6bf519fb1558e22bb1cac0b58d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10570-016-0859-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10570-016-0859-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Li, Dapeng</creatorcontrib><creatorcontrib>Feng, Lu</creatorcontrib><creatorcontrib>Liu, Keran</creatorcontrib><creatorcontrib>Cheng, Yi</creatorcontrib><creatorcontrib>Hou, Ning</creatorcontrib><creatorcontrib>Li, Chunyan</creatorcontrib><title>Optimization of cold-active CMCase production by psychrotrophic Sphingomonas sp. FLX-7 from the cold region of China</title><title>Cellulose (London)</title><addtitle>Cellulose</addtitle><description>Organic solid waste consists of 70 % biodegradables, such as cellulose, which is unable to be degraded quickly and efficiently in the cold region of China. Psychrotrophic cellulose-degrading bacteria play an important role in cellulose degradation at low temperatures. In this study, a psychrotrophic cellulose-degrading FLX-7 strain with high carboxymethyl cellulase (CMCase) activity was isolated from the soil of a solid waste landfill under the environmental temperature of −10 °C. The FLX-7 strain was identified as Sphingobacterium sp. based on morphology and physiological and biochemical properties as well as 16S rRNA gene sequence analysis. To improve the cold-active CMCase production of FLX-7, fermentation conditions were optimized using response surface methodology based on a Box–Behnken design. The maximum CMCase activity (16.95 U/mL) was obtained at optimal fermentation conditions (inoculum size 2.1 %, temperature 10.4 °C, incubation time 73.5 h, and pH 7.2). The optimum pH and temperature for enzymatic reaction of cold-active CMCase were 5.0 and 25 °C, respectively. The cold-active CMCase was stable at a wide pH range of 5.0–9.0 and temperature range of 10–30 °C. The FLX-7 strain could be distributed via a psychrotrophic cellulose-degrading strain library and applied more broadly to organic solid waste composting in cold regions.</description><subject>ambient temperature</subject><subject>bacteria</subject><subject>Bioorganic Chemistry</subject><subject>Cellulase</subject><subject>Cellulose</subject><subject>Ceramics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cold</subject><subject>cold zones</subject><subject>Composites</subject><subject>Composting</subject><subject>Degradation</subject><subject>endo-1,4-beta-glucanase</subject><subject>enzymatic reactions</subject><subject>Fermentation</subject><subject>Glass</subject><subject>Inoculum</subject><subject>landfills</subject><subject>Morphology</subject><subject>Natural Materials</subject><subject>nucleotide sequences</subject><subject>Optimization</subject><subject>Organic Chemistry</subject><subject>Original Paper</subject><subject>Physical Chemistry</subject><subject>Polymer Sciences</subject><subject>Response surface methodology</subject><subject>ribosomal RNA</subject><subject>sequence analysis</subject><subject>soil</subject><subject>solid wastes</subject><subject>Sphingobacterium</subject><subject>Sphingomonas</subject><subject>Sustainable Development</subject><subject>Waste management industry</subject><issn>0969-0239</issn><issn>1572-882X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kE1LAzEQhoMoWD9-gCcDnqOTdLPZHGXxCyo9VMFbSLJJu8Vu1mQr1F9v6grevMxcnucd5kXogsI1BRA3iQIXQICWBCouSXGAJpQLRqqKvR2iCchSEmBTeYxOUloDgBSMTtAw74d2037poQ0dDh7b8N4QbYf20-H6udbJ4T6GZmt_ALPDfdrZVQxDDP2qtXiRZ7cMm9DphFN_je9nb0RgH8MGDyv3k4ejW_7G15nWZ-jI6_fkzn_3KXq9v3upH8ls_vBU386InQoxECM5q3TJC8ZLy2jDZcFNYalpSiYk99Z5bVxpPKfSG8p55RgzhlptwfCqmZ6iqzE3f_CxdWlQ67CNXT6pGONS0oKVVaboSNkYUorOqz62Gx13ioLal6vGclUuV-3LVUV22OikzHZLF_-S_5MuR8nroPQytkm9LlgGAChUVMjpN9CYhrA</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Li, Dapeng</creator><creator>Feng, Lu</creator><creator>Liu, Keran</creator><creator>Cheng, Yi</creator><creator>Hou, Ning</creator><creator>Li, Chunyan</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20160401</creationdate><title>Optimization of cold-active CMCase production by psychrotrophic Sphingomonas sp. FLX-7 from the cold region of China</title><author>Li, Dapeng ; Feng, Lu ; Liu, Keran ; Cheng, Yi ; Hou, Ning ; Li, Chunyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-b9528a654256c21d5945b4c1bd62795fcefabe6bf519fb1558e22bb1cac0b58d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>ambient temperature</topic><topic>bacteria</topic><topic>Bioorganic Chemistry</topic><topic>Cellulase</topic><topic>Cellulose</topic><topic>Ceramics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cold</topic><topic>cold zones</topic><topic>Composites</topic><topic>Composting</topic><topic>Degradation</topic><topic>endo-1,4-beta-glucanase</topic><topic>enzymatic reactions</topic><topic>Fermentation</topic><topic>Glass</topic><topic>Inoculum</topic><topic>landfills</topic><topic>Morphology</topic><topic>Natural Materials</topic><topic>nucleotide sequences</topic><topic>Optimization</topic><topic>Organic Chemistry</topic><topic>Original Paper</topic><topic>Physical Chemistry</topic><topic>Polymer Sciences</topic><topic>Response surface methodology</topic><topic>ribosomal RNA</topic><topic>sequence analysis</topic><topic>soil</topic><topic>solid wastes</topic><topic>Sphingobacterium</topic><topic>Sphingomonas</topic><topic>Sustainable Development</topic><topic>Waste management industry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Dapeng</creatorcontrib><creatorcontrib>Feng, Lu</creatorcontrib><creatorcontrib>Liu, Keran</creatorcontrib><creatorcontrib>Cheng, Yi</creatorcontrib><creatorcontrib>Hou, Ning</creatorcontrib><creatorcontrib>Li, Chunyan</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Cellulose (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Dapeng</au><au>Feng, Lu</au><au>Liu, Keran</au><au>Cheng, Yi</au><au>Hou, Ning</au><au>Li, Chunyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of cold-active CMCase production by psychrotrophic Sphingomonas sp. FLX-7 from the cold region of China</atitle><jtitle>Cellulose (London)</jtitle><stitle>Cellulose</stitle><date>2016-04-01</date><risdate>2016</risdate><volume>23</volume><issue>2</issue><spage>1335</spage><epage>1347</epage><pages>1335-1347</pages><issn>0969-0239</issn><eissn>1572-882X</eissn><abstract>Organic solid waste consists of 70 % biodegradables, such as cellulose, which is unable to be degraded quickly and efficiently in the cold region of China. Psychrotrophic cellulose-degrading bacteria play an important role in cellulose degradation at low temperatures. In this study, a psychrotrophic cellulose-degrading FLX-7 strain with high carboxymethyl cellulase (CMCase) activity was isolated from the soil of a solid waste landfill under the environmental temperature of −10 °C. The FLX-7 strain was identified as Sphingobacterium sp. based on morphology and physiological and biochemical properties as well as 16S rRNA gene sequence analysis. To improve the cold-active CMCase production of FLX-7, fermentation conditions were optimized using response surface methodology based on a Box–Behnken design. The maximum CMCase activity (16.95 U/mL) was obtained at optimal fermentation conditions (inoculum size 2.1 %, temperature 10.4 °C, incubation time 73.5 h, and pH 7.2). The optimum pH and temperature for enzymatic reaction of cold-active CMCase were 5.0 and 25 °C, respectively. The cold-active CMCase was stable at a wide pH range of 5.0–9.0 and temperature range of 10–30 °C. The FLX-7 strain could be distributed via a psychrotrophic cellulose-degrading strain library and applied more broadly to organic solid waste composting in cold regions.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10570-016-0859-4</doi><tpages>13</tpages></addata></record> |
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| subjects | ambient temperature bacteria Bioorganic Chemistry Cellulase Cellulose Ceramics Chemistry Chemistry and Materials Science Cold cold zones Composites Composting Degradation endo-1,4-beta-glucanase enzymatic reactions Fermentation Glass Inoculum landfills Morphology Natural Materials nucleotide sequences Optimization Organic Chemistry Original Paper Physical Chemistry Polymer Sciences Response surface methodology ribosomal RNA sequence analysis soil solid wastes Sphingobacterium Sphingomonas Sustainable Development Waste management industry |
| title | Optimization of cold-active CMCase production by psychrotrophic Sphingomonas sp. FLX-7 from the cold region of China |
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