Cellulose-water interactions during enzymatic hydrolysis as studied by time domain NMR
The different states and locations of water within the cellulose matrix can be studied by the use of time domain low field NMR. In this work we show how the state and location of water associated with cellulose in filter paper fibers are affected by enzymatic hydrolysis. Three locations of water wer...
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| Veröffentlicht in: | Cellulose (London) 2008, Vol.15 (5), p.703-710 |
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| creator | Felby, Claus Thygesen, Lisbeth G Kristensen, Jan B Jørgensen, Henning Elder, Thomas |
| description | The different states and locations of water within the cellulose matrix can be studied by the use of time domain low field NMR. In this work we show how the state and location of water associated with cellulose in filter paper fibers are affected by enzymatic hydrolysis. Three locations of water were identified in the filter paper; (1) bound water associated with the microfibril surfaces and (2) water in the cell wall or cellulose matrix and (3) capillary water in the lumens and between fibers. The different mechanisms of cellulase enzymes can be seen in their effect on the cellulose-water interactions and the synergistic effects between endo- and exo enzymes can be easily detected by time domain NMR. An interesting observation is that it is possible to link the state and location of water within the cellulose fiber with structural changes upon enzymatic hydrolysis. |
| doi_str_mv | 10.1007/s10570-008-9222-8 |
| format | Article |
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In this work we show how the state and location of water associated with cellulose in filter paper fibers are affected by enzymatic hydrolysis. Three locations of water were identified in the filter paper; (1) bound water associated with the microfibril surfaces and (2) water in the cell wall or cellulose matrix and (3) capillary water in the lumens and between fibers. The different mechanisms of cellulase enzymes can be seen in their effect on the cellulose-water interactions and the synergistic effects between endo- and exo enzymes can be easily detected by time domain NMR. An interesting observation is that it is possible to link the state and location of water within the cellulose fiber with structural changes upon enzymatic hydrolysis.</description><identifier>ISSN: 0969-0239</identifier><identifier>EISSN: 1572-882X</identifier><identifier>DOI: 10.1007/s10570-008-9222-8</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Bioorganic Chemistry ; Cellulase ; Cellulose ; Cellulose fibers ; Ceramics ; Chemistry ; Chemistry and Materials Science ; Composites ; enzymatic hydrolysis ; Enzymes ; Filter paper ; Glass ; Hydrolysis ; Lumens ; Natural Materials ; NMR ; Nuclear magnetic resonance ; nuclear magnetic resonance spectroscopy ; Organic Chemistry ; Physical Chemistry ; Polymer Sciences ; spectral analysis ; Sustainable Development ; Time domain analysis ; Water purification</subject><ispartof>Cellulose (London), 2008, Vol.15 (5), p.703-710</ispartof><rights>Springer Science+Business Media B.V. 2008</rights><rights>Cellulose is a copyright of Springer, (2008). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-cfc3c466b541872522a6aedb84939fb50a7f3681417d42041640865ed55d85b33</citedby><cites>FETCH-LOGICAL-c447t-cfc3c466b541872522a6aedb84939fb50a7f3681417d42041640865ed55d85b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10570-008-9222-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10570-008-9222-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Felby, Claus</creatorcontrib><creatorcontrib>Thygesen, Lisbeth G</creatorcontrib><creatorcontrib>Kristensen, Jan B</creatorcontrib><creatorcontrib>Jørgensen, Henning</creatorcontrib><creatorcontrib>Elder, Thomas</creatorcontrib><title>Cellulose-water interactions during enzymatic hydrolysis as studied by time domain NMR</title><title>Cellulose (London)</title><addtitle>Cellulose</addtitle><description>The different states and locations of water within the cellulose matrix can be studied by the use of time domain low field NMR. In this work we show how the state and location of water associated with cellulose in filter paper fibers are affected by enzymatic hydrolysis. Three locations of water were identified in the filter paper; (1) bound water associated with the microfibril surfaces and (2) water in the cell wall or cellulose matrix and (3) capillary water in the lumens and between fibers. The different mechanisms of cellulase enzymes can be seen in their effect on the cellulose-water interactions and the synergistic effects between endo- and exo enzymes can be easily detected by time domain NMR. An interesting observation is that it is possible to link the state and location of water within the cellulose fiber with structural changes upon enzymatic hydrolysis.</description><subject>Bioorganic Chemistry</subject><subject>Cellulase</subject><subject>Cellulose</subject><subject>Cellulose fibers</subject><subject>Ceramics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>enzymatic hydrolysis</subject><subject>Enzymes</subject><subject>Filter paper</subject><subject>Glass</subject><subject>Hydrolysis</subject><subject>Lumens</subject><subject>Natural Materials</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>Organic Chemistry</subject><subject>Physical Chemistry</subject><subject>Polymer Sciences</subject><subject>spectral analysis</subject><subject>Sustainable Development</subject><subject>Time domain analysis</subject><subject>Water purification</subject><issn>0969-0239</issn><issn>1572-882X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1LxDAQhoMouK7-AE8GPFcnadIkR1n8glVBXfEW0iZds3TbNWmR-uttqeDNy8wc3ucdeBA6JXBBAMRlJMAFJAAyUZTSRO6hGeFiOCR930czUJlKgKbqEB3FuAEAJSiZobeFq6quaqJLvkzrAvb1ME3R-qaO2HbB12vs6u9-a1pf4I_ehqbqo4_YRBzbznpncd7j1m8dts3W-Bo_Pjwfo4PSVNGd_O45Wt1cvy7ukuXT7f3iapkUjIk2KcoiLViW5ZwRKSin1GTG2Vwylaoy52BEmWaSMCIso8BIxkBm3FnOreR5ms7R-dS7C81n52KrN00X6uGlppQrqahSckiRKVWEJsbgSr0LfmtCrwnoUZ-e9OlBnx716ZGhExN3owMX_pr_g84mqDSNNuvgo169UCDpoJvKDGT6A1xde38</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Felby, Claus</creator><creator>Thygesen, Lisbeth G</creator><creator>Kristensen, Jan B</creator><creator>Jørgensen, Henning</creator><creator>Elder, Thomas</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>2008</creationdate><title>Cellulose-water interactions during enzymatic hydrolysis as studied by time domain NMR</title><author>Felby, Claus ; Thygesen, Lisbeth G ; Kristensen, Jan B ; Jørgensen, Henning ; Elder, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-cfc3c466b541872522a6aedb84939fb50a7f3681417d42041640865ed55d85b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bioorganic Chemistry</topic><topic>Cellulase</topic><topic>Cellulose</topic><topic>Cellulose fibers</topic><topic>Ceramics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>enzymatic hydrolysis</topic><topic>Enzymes</topic><topic>Filter paper</topic><topic>Glass</topic><topic>Hydrolysis</topic><topic>Lumens</topic><topic>Natural Materials</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>Organic Chemistry</topic><topic>Physical Chemistry</topic><topic>Polymer Sciences</topic><topic>spectral analysis</topic><topic>Sustainable Development</topic><topic>Time domain analysis</topic><topic>Water purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Felby, Claus</creatorcontrib><creatorcontrib>Thygesen, Lisbeth G</creatorcontrib><creatorcontrib>Kristensen, Jan B</creatorcontrib><creatorcontrib>Jørgensen, Henning</creatorcontrib><creatorcontrib>Elder, Thomas</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Cellulose (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Felby, Claus</au><au>Thygesen, Lisbeth G</au><au>Kristensen, Jan B</au><au>Jørgensen, Henning</au><au>Elder, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellulose-water interactions during enzymatic hydrolysis as studied by time domain NMR</atitle><jtitle>Cellulose (London)</jtitle><stitle>Cellulose</stitle><date>2008</date><risdate>2008</risdate><volume>15</volume><issue>5</issue><spage>703</spage><epage>710</epage><pages>703-710</pages><issn>0969-0239</issn><eissn>1572-882X</eissn><abstract>The different states and locations of water within the cellulose matrix can be studied by the use of time domain low field NMR. 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| source | Springer Nature - Complete Springer Journals |
| subjects | Bioorganic Chemistry Cellulase Cellulose Cellulose fibers Ceramics Chemistry Chemistry and Materials Science Composites enzymatic hydrolysis Enzymes Filter paper Glass Hydrolysis Lumens Natural Materials NMR Nuclear magnetic resonance nuclear magnetic resonance spectroscopy Organic Chemistry Physical Chemistry Polymer Sciences spectral analysis Sustainable Development Time domain analysis Water purification |
| title | Cellulose-water interactions during enzymatic hydrolysis as studied by time domain NMR |
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