Improved silica xerogel film processing for MALDI-TOF–MS quantitative analysis of peptides and small molecules
In this work, sol–gel derived silica films were prepared for direct desorption/ionization of organic compounds in MALDI-TOF–MS analysis with the aim of improving method precision and of reducing interfering signals at low m/z values. Two commonly used MALDI matrices, 2,5-dihydroxybenzoic acid (DHB)...
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| Veröffentlicht in: | Journal of sol-gel science and technology 2011-12, Vol.60 (3), p.359-365 |
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| creator | Careri, M. Elviri, L. Lorenzi, A. Mangia, A. Penna, A. Predieri, G. |
| description | In this work, sol–gel derived silica films were prepared for direct desorption/ionization of organic compounds in MALDI-TOF–MS analysis with the aim of improving method precision and of reducing interfering signals at low m/z values. Two commonly used MALDI matrices, 2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (CHCA), were incorporated into the sol–gel network in order to absorb laser energy and to induce analyte desorption/ionization with low or absent background signals in the mass spectra. To achieve a reproducible xerogel film formation, experimental parameters for its deposition were optimized. The gel matrices were characterized by Fourier Transform Infrared (FT-IR) spectroscopy, X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) analysis. The results proved the embedding of the matrix molecules in a disperse form into the homogeneous sol–gel material. The sol–gel matrix was then tested for the qualitative and quantitative analysis of two reference peptides, such as Bradykinin and P
14
R. In addition, spectral quality and method performance were assessed for quantitation of melamine, a low-molecular weight compound of food safety concern. In all cases, high quality spectra and excellent mass accuracy (between 3.5 and 13 ppm) were observed. Furthermore, the experimental results evidenced a significant improvement of the measurement repeatability on spot and between spots (relative standard deviation |
| doi_str_mv | 10.1007/s10971-011-2555-z |
| format | Article |
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14
R. In addition, spectral quality and method performance were assessed for quantitation of melamine, a low-molecular weight compound of food safety concern. In all cases, high quality spectra and excellent mass accuracy (between 3.5 and 13 ppm) were observed. Furthermore, the experimental results evidenced a significant improvement of the measurement repeatability on spot and between spots (relative standard deviation <10%), with respect to the traditional dried-droplet sample deposition method. Good sensitivity and linearity in the concentration range explored were obtained for peptides and melamine, demonstrating the suitability of the sol–gel-based matrix to be used for quantitative analysis.</description><identifier>ISSN: 0928-0707</identifier><identifier>EISSN: 1573-4846</identifier><identifier>DOI: 10.1007/s10971-011-2555-z</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Ceramics ; Chemistry and Materials Science ; Composites ; Deposition ; Desorption ; Dihydroxybenzoic acid ; Fourier transforms ; Glass ; Hydroxycinnamic acid ; Infrared analysis ; Infrared spectroscopy ; Inorganic Chemistry ; Ionization ; Ions ; Linearity ; Mass spectra ; Materials Science ; Melamine ; Nanotechnology ; Natural Materials ; Optical and Electronic Materials ; Organic compounds ; Original Paper ; Peptides ; Product safety ; Qualitative analysis ; Quantitative analysis ; Reproducibility ; Scanning electron microscopy ; Silica gel ; Silicon dioxide ; Sol-gel processes ; Spectrum analysis ; X-ray diffraction ; Xerogels</subject><ispartof>Journal of sol-gel science and technology, 2011-12, Vol.60 (3), p.359-365</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><rights>Journal of Sol-Gel Science and Technology is a copyright of Springer, (2011). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-804b811205f8c645217bf635641ad1d71b2f17479f01f20696bb490d96bfe1853</citedby><cites>FETCH-LOGICAL-c316t-804b811205f8c645217bf635641ad1d71b2f17479f01f20696bb490d96bfe1853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10971-011-2555-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10971-011-2555-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Careri, M.</creatorcontrib><creatorcontrib>Elviri, L.</creatorcontrib><creatorcontrib>Lorenzi, A.</creatorcontrib><creatorcontrib>Mangia, A.</creatorcontrib><creatorcontrib>Penna, A.</creatorcontrib><creatorcontrib>Predieri, G.</creatorcontrib><title>Improved silica xerogel film processing for MALDI-TOF–MS quantitative analysis of peptides and small molecules</title><title>Journal of sol-gel science and technology</title><addtitle>J Sol-Gel Sci Technol</addtitle><description>In this work, sol–gel derived silica films were prepared for direct desorption/ionization of organic compounds in MALDI-TOF–MS analysis with the aim of improving method precision and of reducing interfering signals at low m/z values. Two commonly used MALDI matrices, 2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (CHCA), were incorporated into the sol–gel network in order to absorb laser energy and to induce analyte desorption/ionization with low or absent background signals in the mass spectra. To achieve a reproducible xerogel film formation, experimental parameters for its deposition were optimized. The gel matrices were characterized by Fourier Transform Infrared (FT-IR) spectroscopy, X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) analysis. The results proved the embedding of the matrix molecules in a disperse form into the homogeneous sol–gel material. The sol–gel matrix was then tested for the qualitative and quantitative analysis of two reference peptides, such as Bradykinin and P
14
R. In addition, spectral quality and method performance were assessed for quantitation of melamine, a low-molecular weight compound of food safety concern. In all cases, high quality spectra and excellent mass accuracy (between 3.5 and 13 ppm) were observed. Furthermore, the experimental results evidenced a significant improvement of the measurement repeatability on spot and between spots (relative standard deviation <10%), with respect to the traditional dried-droplet sample deposition method. Good sensitivity and linearity in the concentration range explored were obtained for peptides and melamine, demonstrating the suitability of the sol–gel-based matrix to be used for quantitative analysis.</description><subject>Ceramics</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Deposition</subject><subject>Desorption</subject><subject>Dihydroxybenzoic acid</subject><subject>Fourier transforms</subject><subject>Glass</subject><subject>Hydroxycinnamic acid</subject><subject>Infrared analysis</subject><subject>Infrared spectroscopy</subject><subject>Inorganic Chemistry</subject><subject>Ionization</subject><subject>Ions</subject><subject>Linearity</subject><subject>Mass spectra</subject><subject>Materials Science</subject><subject>Melamine</subject><subject>Nanotechnology</subject><subject>Natural Materials</subject><subject>Optical and Electronic Materials</subject><subject>Organic compounds</subject><subject>Original Paper</subject><subject>Peptides</subject><subject>Product safety</subject><subject>Qualitative analysis</subject><subject>Quantitative analysis</subject><subject>Reproducibility</subject><subject>Scanning electron microscopy</subject><subject>Silica gel</subject><subject>Silicon dioxide</subject><subject>Sol-gel processes</subject><subject>Spectrum analysis</subject><subject>X-ray diffraction</subject><subject>Xerogels</subject><issn>0928-0707</issn><issn>1573-4846</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kE1OwzAQhS0EEuXnAOwssTbMuHacLBG_lYpYAGvLSezKyGmCnSLoijtwQ06CqyKxYvWkmfeeZj5CThDOEECdJ4RKIQNExqWUbL1DJijVlIlSFLtkAhUvGShQ--QgpRcAkALVhAyzboj9m21p8sE3hr7b2C9soM6HjuZVY1PyywV1faT3F_OrGXt6uPn-_Lp_pK8rsxz9aEb_ZqlZmvCRfKK9o4MdRt_alIe5tzMh0K4PtlkFm47InjMh2eNfPSTPN9dPl3ds_nA7u7yYs2aKxchKEHWJyEG6simE5KhqV0xlIdC02CqsuUMlVOUAHYeiKupaVNBmdRZLOT0kp9ve_MPryqZRv_SrmI9MmnNZSQHINy7cuprYpxSt00P0nYkfGkFvwOotWJ3B6g1Yvc4Zvs2k7F0ubPxr_j_0A70SfHg</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Careri, M.</creator><creator>Elviri, L.</creator><creator>Lorenzi, A.</creator><creator>Mangia, A.</creator><creator>Penna, A.</creator><creator>Predieri, G.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>L6V</scope><scope>M7S</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20111201</creationdate><title>Improved silica xerogel film processing for MALDI-TOF–MS quantitative analysis of peptides and small molecules</title><author>Careri, M. ; Elviri, L. ; Lorenzi, A. ; Mangia, A. ; Penna, A. ; Predieri, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-804b811205f8c645217bf635641ad1d71b2f17479f01f20696bb490d96bfe1853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Ceramics</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Deposition</topic><topic>Desorption</topic><topic>Dihydroxybenzoic acid</topic><topic>Fourier transforms</topic><topic>Glass</topic><topic>Hydroxycinnamic acid</topic><topic>Infrared analysis</topic><topic>Infrared spectroscopy</topic><topic>Inorganic Chemistry</topic><topic>Ionization</topic><topic>Ions</topic><topic>Linearity</topic><topic>Mass spectra</topic><topic>Materials Science</topic><topic>Melamine</topic><topic>Nanotechnology</topic><topic>Natural Materials</topic><topic>Optical and Electronic Materials</topic><topic>Organic compounds</topic><topic>Original Paper</topic><topic>Peptides</topic><topic>Product safety</topic><topic>Qualitative analysis</topic><topic>Quantitative analysis</topic><topic>Reproducibility</topic><topic>Scanning electron microscopy</topic><topic>Silica gel</topic><topic>Silicon dioxide</topic><topic>Sol-gel processes</topic><topic>Spectrum analysis</topic><topic>X-ray diffraction</topic><topic>Xerogels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Careri, M.</creatorcontrib><creatorcontrib>Elviri, L.</creatorcontrib><creatorcontrib>Lorenzi, A.</creatorcontrib><creatorcontrib>Mangia, A.</creatorcontrib><creatorcontrib>Penna, A.</creatorcontrib><creatorcontrib>Predieri, G.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>Engineering Database</collection><collection>Materials Science Collection</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><jtitle>Journal of sol-gel science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Careri, M.</au><au>Elviri, L.</au><au>Lorenzi, A.</au><au>Mangia, A.</au><au>Penna, A.</au><au>Predieri, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved silica xerogel film processing for MALDI-TOF–MS quantitative analysis of peptides and small molecules</atitle><jtitle>Journal of sol-gel science and technology</jtitle><stitle>J Sol-Gel Sci Technol</stitle><date>2011-12-01</date><risdate>2011</risdate><volume>60</volume><issue>3</issue><spage>359</spage><epage>365</epage><pages>359-365</pages><issn>0928-0707</issn><eissn>1573-4846</eissn><abstract>In this work, sol–gel derived silica films were prepared for direct desorption/ionization of organic compounds in MALDI-TOF–MS analysis with the aim of improving method precision and of reducing interfering signals at low m/z values. Two commonly used MALDI matrices, 2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (CHCA), were incorporated into the sol–gel network in order to absorb laser energy and to induce analyte desorption/ionization with low or absent background signals in the mass spectra. To achieve a reproducible xerogel film formation, experimental parameters for its deposition were optimized. The gel matrices were characterized by Fourier Transform Infrared (FT-IR) spectroscopy, X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) analysis. The results proved the embedding of the matrix molecules in a disperse form into the homogeneous sol–gel material. The sol–gel matrix was then tested for the qualitative and quantitative analysis of two reference peptides, such as Bradykinin and P
14
R. In addition, spectral quality and method performance were assessed for quantitation of melamine, a low-molecular weight compound of food safety concern. In all cases, high quality spectra and excellent mass accuracy (between 3.5 and 13 ppm) were observed. Furthermore, the experimental results evidenced a significant improvement of the measurement repeatability on spot and between spots (relative standard deviation <10%), with respect to the traditional dried-droplet sample deposition method. Good sensitivity and linearity in the concentration range explored were obtained for peptides and melamine, demonstrating the suitability of the sol–gel-based matrix to be used for quantitative analysis.</abstract><cop>Boston</cop><pub>Springer US</pub><doi>10.1007/s10971-011-2555-z</doi><tpages>7</tpages></addata></record> |
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| subjects | Ceramics Chemistry and Materials Science Composites Deposition Desorption Dihydroxybenzoic acid Fourier transforms Glass Hydroxycinnamic acid Infrared analysis Infrared spectroscopy Inorganic Chemistry Ionization Ions Linearity Mass spectra Materials Science Melamine Nanotechnology Natural Materials Optical and Electronic Materials Organic compounds Original Paper Peptides Product safety Qualitative analysis Quantitative analysis Reproducibility Scanning electron microscopy Silica gel Silicon dioxide Sol-gel processes Spectrum analysis X-ray diffraction Xerogels |
| title | Improved silica xerogel film processing for MALDI-TOF–MS quantitative analysis of peptides and small molecules |
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