Expression patterns of genes involved in starch biosynthesis during seed development in bread wheat (Triticum aestivum)
In agricultural crops, seed growth is important for high grain yield. Starch contributes about 50–80 % of the dry weight of seed, and its quality affects both processing and nutrition quality. Despite the wider importance of starch metabolism, the genes involved have not been given much attention or...
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| Veröffentlicht in: | Molecular breeding 2015-09, Vol.35 (9), p.1-9, Article 184 |
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| creator | Singh, Anuradha Kumar, Pankaj Sharma, Monica Tuli, Rakesh Dhaliwal, Harcharan S. Chaudhury, Ashok Pal, Dharam Roy, Joy |
| description | In agricultural crops, seed growth is important for high grain yield. Starch contributes about 50–80 % of the dry weight of seed, and its quality affects both processing and nutrition quality. Despite the wider importance of starch metabolism, the genes involved have not been given much attention or exploited for their use in molecular breeding. Therefore, it is of great interest to analyze the expression of genes involved in starch metabolism for improvement of starch-related traits through molecular breeding. In this study, a quantitative gene expression analysis of 25 starch metabolism genes was conducted in three bread wheat (Triticum aestivum) genotypes differing in yield- and starch-related traits at five seed developmental stages, i.e., 7, 14, 21, 28, and 35 days after anthesis. Their sequences were physically mapped to chromosomes using the wheat genome sequence data through in silico analysis. Their expression data showed dynamic variation during seed development in wheat genotypes. The 25 genes were divided into four groups depending on their expression patterns during seed development. For example, one group was characterized by a high level of expression at early and middle stages as exhibited by different isoforms of starch synthases, starch-branching enzymes, isoamylase, and transcription factors (TaRSR1 and SPA). The enzymes of these genes are key factors in starch biosynthesis. The starch metabolism genes with high expression levels will be sequenced in a wheat germplasm set to develop single nucleotide polymorphism markers for improvement of yield- and starch-related traits through molecular breeding approaches. |
| doi_str_mv | 10.1007/s11032-015-0371-9 |
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Starch contributes about 50–80 % of the dry weight of seed, and its quality affects both processing and nutrition quality. Despite the wider importance of starch metabolism, the genes involved have not been given much attention or exploited for their use in molecular breeding. Therefore, it is of great interest to analyze the expression of genes involved in starch metabolism for improvement of starch-related traits through molecular breeding. In this study, a quantitative gene expression analysis of 25 starch metabolism genes was conducted in three bread wheat (Triticum aestivum) genotypes differing in yield- and starch-related traits at five seed developmental stages, i.e., 7, 14, 21, 28, and 35 days after anthesis. Their sequences were physically mapped to chromosomes using the wheat genome sequence data through in silico analysis. Their expression data showed dynamic variation during seed development in wheat genotypes. The 25 genes were divided into four groups depending on their expression patterns during seed development. For example, one group was characterized by a high level of expression at early and middle stages as exhibited by different isoforms of starch synthases, starch-branching enzymes, isoamylase, and transcription factors (TaRSR1 and SPA). The enzymes of these genes are key factors in starch biosynthesis. The starch metabolism genes with high expression levels will be sequenced in a wheat germplasm set to develop single nucleotide polymorphism markers for improvement of yield- and starch-related traits through molecular breeding approaches.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-015-0371-9</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Bread ; Breeding ; Chromosomes ; Crop yield ; Data analysis ; Developmental stages ; Enzymes ; Gene expression ; Gene polymorphism ; Gene sequencing ; Genes ; Genomes ; Genotypes ; Germplasm ; Isoamylase ; Isoforms ; Life Sciences ; Metabolism ; Molecular biology ; Nucleotide sequence ; Nucleotides ; Nutrition ; Nutritive value ; Plant biology ; Plant breeding ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polymorphism ; Short Communication ; Single-nucleotide polymorphism ; Starch ; Starch-branching enzymes ; Transcription factors ; Triticum aestivum ; Wheat</subject><ispartof>Molecular breeding, 2015-09, Vol.35 (9), p.1-9, Article 184</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><rights>Molecular Breeding is a copyright of Springer, (2015). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-f9659be0444a457cdf40c84f38685bd6f9a3b002528be9c2507e8747f464551c3</citedby><cites>FETCH-LOGICAL-c340t-f9659be0444a457cdf40c84f38685bd6f9a3b002528be9c2507e8747f464551c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-015-0371-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-015-0371-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Singh, Anuradha</creatorcontrib><creatorcontrib>Kumar, Pankaj</creatorcontrib><creatorcontrib>Sharma, Monica</creatorcontrib><creatorcontrib>Tuli, Rakesh</creatorcontrib><creatorcontrib>Dhaliwal, Harcharan S.</creatorcontrib><creatorcontrib>Chaudhury, Ashok</creatorcontrib><creatorcontrib>Pal, Dharam</creatorcontrib><creatorcontrib>Roy, Joy</creatorcontrib><title>Expression patterns of genes involved in starch biosynthesis during seed development in bread wheat (Triticum aestivum)</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>In agricultural crops, seed growth is important for high grain yield. Starch contributes about 50–80 % of the dry weight of seed, and its quality affects both processing and nutrition quality. Despite the wider importance of starch metabolism, the genes involved have not been given much attention or exploited for their use in molecular breeding. Therefore, it is of great interest to analyze the expression of genes involved in starch metabolism for improvement of starch-related traits through molecular breeding. In this study, a quantitative gene expression analysis of 25 starch metabolism genes was conducted in three bread wheat (Triticum aestivum) genotypes differing in yield- and starch-related traits at five seed developmental stages, i.e., 7, 14, 21, 28, and 35 days after anthesis. Their sequences were physically mapped to chromosomes using the wheat genome sequence data through in silico analysis. Their expression data showed dynamic variation during seed development in wheat genotypes. The 25 genes were divided into four groups depending on their expression patterns during seed development. For example, one group was characterized by a high level of expression at early and middle stages as exhibited by different isoforms of starch synthases, starch-branching enzymes, isoamylase, and transcription factors (TaRSR1 and SPA). The enzymes of these genes are key factors in starch biosynthesis. The starch metabolism genes with high expression levels will be sequenced in a wheat germplasm set to develop single nucleotide polymorphism markers for improvement of yield- and starch-related traits through molecular breeding approaches.</description><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Bread</subject><subject>Breeding</subject><subject>Chromosomes</subject><subject>Crop yield</subject><subject>Data analysis</subject><subject>Developmental stages</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Gene polymorphism</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genotypes</subject><subject>Germplasm</subject><subject>Isoamylase</subject><subject>Isoforms</subject><subject>Life Sciences</subject><subject>Metabolism</subject><subject>Molecular biology</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Nutrition</subject><subject>Nutritive value</subject><subject>Plant biology</subject><subject>Plant breeding</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polymorphism</subject><subject>Short Communication</subject><subject>Single-nucleotide polymorphism</subject><subject>Starch</subject><subject>Starch-branching enzymes</subject><subject>Transcription factors</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kEtv1DAUhSMEEqXwA1hhiQ0sAtevOF6iqjykSixo15aTXM-4momDrzNt_z0eBYkdq3sW5ztX-prmLYdPHMB8Js5Biha4bkEa3tpnzQXXRrTW9P3zmmUPrTRKvmxeEd1DZWzXXTQP149LRqKYZrb4UjDPxFJgO5yRWJxP6XDCqQZGxedxz4aY6Gkue6RIbFpznHeMsFYmPOEhLUecy7k-ZPQTe9ijL-zDbY4ljuuReaQST-vx4-vmRfAHwjd_72Vz9_X69up7e_Pz24-rLzftKBWUNthO2wFBKeWVNuMUFIy9CrLvej1MXbBeDgBCi35AOwoNBnujTFCd0pqP8rJ5v-0uOf1e63d3n9Y815dOCG2V1aITtcW31pgTUcbglhyPPj85Du7s121-XfXrzn6drYzYGFrOEjD_W_4f9G6Dgk_O73Ikd_dLAO8AuNTKKvkHlq-H8A</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Singh, Anuradha</creator><creator>Kumar, Pankaj</creator><creator>Sharma, Monica</creator><creator>Tuli, Rakesh</creator><creator>Dhaliwal, Harcharan S.</creator><creator>Chaudhury, Ashok</creator><creator>Pal, Dharam</creator><creator>Roy, Joy</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20150901</creationdate><title>Expression patterns of genes involved in starch biosynthesis during seed development in bread wheat (Triticum aestivum)</title><author>Singh, Anuradha ; Kumar, Pankaj ; Sharma, Monica ; Tuli, Rakesh ; Dhaliwal, Harcharan S. ; Chaudhury, Ashok ; Pal, Dharam ; Roy, Joy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-f9659be0444a457cdf40c84f38685bd6f9a3b002528be9c2507e8747f464551c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Bread</topic><topic>Breeding</topic><topic>Chromosomes</topic><topic>Crop yield</topic><topic>Data analysis</topic><topic>Developmental stages</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Gene polymorphism</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genotypes</topic><topic>Germplasm</topic><topic>Isoamylase</topic><topic>Isoforms</topic><topic>Life Sciences</topic><topic>Metabolism</topic><topic>Molecular biology</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Nutrition</topic><topic>Nutritive value</topic><topic>Plant biology</topic><topic>Plant breeding</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polymorphism</topic><topic>Short Communication</topic><topic>Single-nucleotide polymorphism</topic><topic>Starch</topic><topic>Starch-branching enzymes</topic><topic>Transcription factors</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Anuradha</creatorcontrib><creatorcontrib>Kumar, Pankaj</creatorcontrib><creatorcontrib>Sharma, Monica</creatorcontrib><creatorcontrib>Tuli, Rakesh</creatorcontrib><creatorcontrib>Dhaliwal, Harcharan S.</creatorcontrib><creatorcontrib>Chaudhury, Ashok</creatorcontrib><creatorcontrib>Pal, Dharam</creatorcontrib><creatorcontrib>Roy, Joy</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Anuradha</au><au>Kumar, Pankaj</au><au>Sharma, Monica</au><au>Tuli, Rakesh</au><au>Dhaliwal, Harcharan S.</au><au>Chaudhury, Ashok</au><au>Pal, Dharam</au><au>Roy, Joy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression patterns of genes involved in starch biosynthesis during seed development in bread wheat (Triticum aestivum)</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2015-09-01</date><risdate>2015</risdate><volume>35</volume><issue>9</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><artnum>184</artnum><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>In agricultural crops, seed growth is important for high grain yield. Starch contributes about 50–80 % of the dry weight of seed, and its quality affects both processing and nutrition quality. Despite the wider importance of starch metabolism, the genes involved have not been given much attention or exploited for their use in molecular breeding. Therefore, it is of great interest to analyze the expression of genes involved in starch metabolism for improvement of starch-related traits through molecular breeding. In this study, a quantitative gene expression analysis of 25 starch metabolism genes was conducted in three bread wheat (Triticum aestivum) genotypes differing in yield- and starch-related traits at five seed developmental stages, i.e., 7, 14, 21, 28, and 35 days after anthesis. Their sequences were physically mapped to chromosomes using the wheat genome sequence data through in silico analysis. Their expression data showed dynamic variation during seed development in wheat genotypes. The 25 genes were divided into four groups depending on their expression patterns during seed development. For example, one group was characterized by a high level of expression at early and middle stages as exhibited by different isoforms of starch synthases, starch-branching enzymes, isoamylase, and transcription factors (TaRSR1 and SPA). The enzymes of these genes are key factors in starch biosynthesis. The starch metabolism genes with high expression levels will be sequenced in a wheat germplasm set to develop single nucleotide polymorphism markers for improvement of yield- and starch-related traits through molecular breeding approaches.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11032-015-0371-9</doi><tpages>9</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Biosynthesis Biotechnology Bread Breeding Chromosomes Crop yield Data analysis Developmental stages Enzymes Gene expression Gene polymorphism Gene sequencing Genes Genomes Genotypes Germplasm Isoamylase Isoforms Life Sciences Metabolism Molecular biology Nucleotide sequence Nucleotides Nutrition Nutritive value Plant biology Plant breeding Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polymorphism Short Communication Single-nucleotide polymorphism Starch Starch-branching enzymes Transcription factors Triticum aestivum Wheat |
| title | Expression patterns of genes involved in starch biosynthesis during seed development in bread wheat (Triticum aestivum) |
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