Development of microsatellite markers in canary seed (Phalaris canariensis L.)
Annual canarygrass, commonly known as canary seed (Phalaris canariensis L.), is a self-pollinated diploid cereal (2n = 12) with a genome size of 3,800 Mbp. Canary seed is presently used for bird-feed with a potential to develop it for human consumption. Marker-assisted selection can be used to accel...
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| Veröffentlicht in: | Molecular breeding 2011-12, Vol.28 (4), p.611-621 |
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| creator | Li, Jingzhao Båga, Monica Hucl, Pierre Chibbar, Ravindra N |
| description | Annual canarygrass, commonly known as canary seed (Phalaris canariensis L.), is a self-pollinated diploid cereal (2n = 12) with a genome size of 3,800 Mbp. Canary seed is presently used for bird-feed with a potential to develop it for human consumption. Marker-assisted selection can be used to accelerate breeding of new canary seed cultivars. Microsatellites or simple sequence repeat (SSR) markers generally show a high degree of polymorphism in different plant genera. FIASCO (Fast Isolation by AFLP of Sequences COntaining repeats) was used to generate microsatellite markers specific for canary seed. An enriched SSR (AG)17 library derived from DNA isolated from a canary seed cultivar (CDC Togo) was produced. Analysis and DNA sequencing of the library resulted in 744 clones from which 132 primer pairs were designed. Seventy-eight functional markers amplified unique products from canary seed DNA. These SSR markers revealed the biodiversity among a panel of 48 canary seed accessions. Polymorphic information content (PIC) values of 37 polymorphic microsatellites ranged from 0.08 to 0.73 with an average of 0.36. |
| doi_str_mv | 10.1007/s11032-010-9513-2 |
| format | Article |
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Canary seed is presently used for bird-feed with a potential to develop it for human consumption. Marker-assisted selection can be used to accelerate breeding of new canary seed cultivars. Microsatellites or simple sequence repeat (SSR) markers generally show a high degree of polymorphism in different plant genera. FIASCO (Fast Isolation by AFLP of Sequences COntaining repeats) was used to generate microsatellite markers specific for canary seed. An enriched SSR (AG)17 library derived from DNA isolated from a canary seed cultivar (CDC Togo) was produced. Analysis and DNA sequencing of the library resulted in 744 clones from which 132 primer pairs were designed. Seventy-eight functional markers amplified unique products from canary seed DNA. These SSR markers revealed the biodiversity among a panel of 48 canary seed accessions. Polymorphic information content (PIC) values of 37 polymorphic microsatellites ranged from 0.08 to 0.73 with an average of 0.36.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-010-9513-2</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Amplified fragment length polymorphism ; Biodiversity ; Biomedical and Life Sciences ; Biotechnology ; clones ; Cultivars ; Deoxyribonucleic acid ; diploidy ; DNA ; DNA libraries ; DNA sequencing ; Gene polymorphism ; Genetic markers ; genome ; Genomes ; Life Sciences ; Marker-assisted selection ; Markers ; microsatellite repeats ; Microsatellites ; Molecular biology ; Phalaris canariensis ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polymorphism ; sequence analysis</subject><ispartof>Molecular breeding, 2011-12, Vol.28 (4), p.611-621</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>Molecular Breeding is a copyright of Springer, (2010). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-55e6304b6cb1feb37d00ef61fbdf1800dd747be61a521ed7167193778bc3d88f3</citedby><cites>FETCH-LOGICAL-c340t-55e6304b6cb1feb37d00ef61fbdf1800dd747be61a521ed7167193778bc3d88f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-010-9513-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-010-9513-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Li, Jingzhao</creatorcontrib><creatorcontrib>Båga, Monica</creatorcontrib><creatorcontrib>Hucl, Pierre</creatorcontrib><creatorcontrib>Chibbar, Ravindra N</creatorcontrib><title>Development of microsatellite markers in canary seed (Phalaris canariensis L.)</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>Annual canarygrass, commonly known as canary seed (Phalaris canariensis L.), is a self-pollinated diploid cereal (2n = 12) with a genome size of 3,800 Mbp. Canary seed is presently used for bird-feed with a potential to develop it for human consumption. Marker-assisted selection can be used to accelerate breeding of new canary seed cultivars. Microsatellites or simple sequence repeat (SSR) markers generally show a high degree of polymorphism in different plant genera. FIASCO (Fast Isolation by AFLP of Sequences COntaining repeats) was used to generate microsatellite markers specific for canary seed. An enriched SSR (AG)17 library derived from DNA isolated from a canary seed cultivar (CDC Togo) was produced. Analysis and DNA sequencing of the library resulted in 744 clones from which 132 primer pairs were designed. Seventy-eight functional markers amplified unique products from canary seed DNA. These SSR markers revealed the biodiversity among a panel of 48 canary seed accessions. Polymorphic information content (PIC) values of 37 polymorphic microsatellites ranged from 0.08 to 0.73 with an average of 0.36.</description><subject>Amplified fragment length polymorphism</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>clones</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>diploidy</subject><subject>DNA</subject><subject>DNA libraries</subject><subject>DNA sequencing</subject><subject>Gene polymorphism</subject><subject>Genetic markers</subject><subject>genome</subject><subject>Genomes</subject><subject>Life Sciences</subject><subject>Marker-assisted selection</subject><subject>Markers</subject><subject>microsatellite repeats</subject><subject>Microsatellites</subject><subject>Molecular biology</subject><subject>Phalaris canariensis</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polymorphism</subject><subject>sequence analysis</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LxDAQhoMouK7-AE8WvOih60zSJulR_IZFBd1zSNvJ2rXbrkkV_PdmqeDN0wzD-8wwD2PHCDMEUBcBEQRPASEtchQp32ETzBVPC6X1buyFhlSoTOyzgxBWEJlCygl7vKYvavvNmroh6V2ybirfBztQ2zYDJWvr38mHpOmSynbWfyeBqE7Ont9sa30TxmlDXYj9fHZ-yPacbQMd_dYpW9zevF7dp_Onu4ery3laiQyGNM9JCshKWZXoqBSqBiAn0ZW1Qw1Q1ypTJUm0OUeqFUqFhVBKl5WotXZiyk7HvRvff3xSGMyq__RdPGk4z4sMgRcypnBMbX8KnpzZ-Ca-9G0QzFabGbWZqM1stRkeGT4yIWa7Jfm_zf9BJyPkbG_sMooxixcOmEXPUmMmxA8OmXg8</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Li, Jingzhao</creator><creator>Båga, Monica</creator><creator>Hucl, Pierre</creator><creator>Chibbar, Ravindra N</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20111201</creationdate><title>Development of microsatellite markers in canary seed (Phalaris canariensis L.)</title><author>Li, Jingzhao ; Båga, Monica ; Hucl, Pierre ; Chibbar, Ravindra N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-55e6304b6cb1feb37d00ef61fbdf1800dd747be61a521ed7167193778bc3d88f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amplified fragment length polymorphism</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>clones</topic><topic>Cultivars</topic><topic>Deoxyribonucleic acid</topic><topic>diploidy</topic><topic>DNA</topic><topic>DNA libraries</topic><topic>DNA sequencing</topic><topic>Gene polymorphism</topic><topic>Genetic markers</topic><topic>genome</topic><topic>Genomes</topic><topic>Life Sciences</topic><topic>Marker-assisted selection</topic><topic>Markers</topic><topic>microsatellite repeats</topic><topic>Microsatellites</topic><topic>Molecular biology</topic><topic>Phalaris canariensis</topic><topic>Plant biology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polymorphism</topic><topic>sequence analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jingzhao</creatorcontrib><creatorcontrib>Båga, Monica</creatorcontrib><creatorcontrib>Hucl, Pierre</creatorcontrib><creatorcontrib>Chibbar, Ravindra N</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jingzhao</au><au>Båga, Monica</au><au>Hucl, Pierre</au><au>Chibbar, Ravindra N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of microsatellite markers in canary seed (Phalaris canariensis L.)</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2011-12-01</date><risdate>2011</risdate><volume>28</volume><issue>4</issue><spage>611</spage><epage>621</epage><pages>611-621</pages><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Annual canarygrass, commonly known as canary seed (Phalaris canariensis L.), is a self-pollinated diploid cereal (2n = 12) with a genome size of 3,800 Mbp. Canary seed is presently used for bird-feed with a potential to develop it for human consumption. Marker-assisted selection can be used to accelerate breeding of new canary seed cultivars. Microsatellites or simple sequence repeat (SSR) markers generally show a high degree of polymorphism in different plant genera. FIASCO (Fast Isolation by AFLP of Sequences COntaining repeats) was used to generate microsatellite markers specific for canary seed. An enriched SSR (AG)17 library derived from DNA isolated from a canary seed cultivar (CDC Togo) was produced. Analysis and DNA sequencing of the library resulted in 744 clones from which 132 primer pairs were designed. Seventy-eight functional markers amplified unique products from canary seed DNA. These SSR markers revealed the biodiversity among a panel of 48 canary seed accessions. Polymorphic information content (PIC) values of 37 polymorphic microsatellites ranged from 0.08 to 0.73 with an average of 0.36.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><doi>10.1007/s11032-010-9513-2</doi><tpages>11</tpages></addata></record> |
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| subjects | Amplified fragment length polymorphism Biodiversity Biomedical and Life Sciences Biotechnology clones Cultivars Deoxyribonucleic acid diploidy DNA DNA libraries DNA sequencing Gene polymorphism Genetic markers genome Genomes Life Sciences Marker-assisted selection Markers microsatellite repeats Microsatellites Molecular biology Phalaris canariensis Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polymorphism sequence analysis |
| title | Development of microsatellite markers in canary seed (Phalaris canariensis L.) |
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