RETRACTED ARTICLE: In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts
Canscora decussata Schult., popularly known as ‘shankhpushpi’, is a threatened medicinal plant. The whole plant is used in Ayurveda for curing insanity, epilepsy and nervous debility. Seasonal and limited availability along with the demand in herbal industries justify the need for developing in vitr...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2015-08, Vol.122 (2), p.509-516 |
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| creator | Gaikwad, Nitin Kundlikrao Moon, Utkarsh Ravindra Bhadoria, Pratapbhanu Singh Mitra, Adinpunya |
| description | Canscora decussata
Schult., popularly known as ‘shankhpushpi’, is a threatened medicinal plant. The whole plant is used in Ayurveda for curing insanity, epilepsy and nervous debility. Seasonal and limited availability along with the demand in herbal industries justify the need for developing in vitro plantlets for sustainable utilization of this plant. In the present study, an efficient protocol for in vitro propagation of
C. decussata
has been developed. Highest shoot induction was observed in the Murashige and Skoog (MS) basal medium supplemented with 0.2 mg l
−1
benzylaminopurine (BAP) when nodal explants were used. Enhanced shoot induction (83.34 %) and multiplication (3.85 ± 0.23 average shoots per explant) were achieved when in vitro grown nodal buds were cultured on MS medium containing optimized BAP along with 1 mg l
−1
naphthaleneacetic acid. Different solvent extracts of in vitro grown plantlets and wild plants were compared for acetylcholinesterase (AChE) inhibitory and antioxidant capacities. Highest AChE inhibitory activity was observed in ethanolic extract of wild plant biomass (IC
50
, 31.70 ± 0.16 µg ml
−1
) followed by ethanolic extract of in vitro plantlets (IC
50
, 44.17 ± 0.42 µg ml
−1
). On a whole, extracts of wild plant as well as in vitro plantlets exhibited the ability to scavenge DPPH and ABTS radicals, reduce ferric iron and protect DNA. These findings suggest that in vitro plantlets of
C. decussata
represent a promising alternative source for the botanicals ‘sankhapushpi’ for alleviating nervous ailments. |
| doi_str_mv | 10.1007/s11240-015-0770-y |
| format | Article |
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Schult., popularly known as ‘shankhpushpi’, is a threatened medicinal plant. The whole plant is used in Ayurveda for curing insanity, epilepsy and nervous debility. Seasonal and limited availability along with the demand in herbal industries justify the need for developing in vitro plantlets for sustainable utilization of this plant. In the present study, an efficient protocol for in vitro propagation of
C. decussata
has been developed. Highest shoot induction was observed in the Murashige and Skoog (MS) basal medium supplemented with 0.2 mg l
−1
benzylaminopurine (BAP) when nodal explants were used. Enhanced shoot induction (83.34 %) and multiplication (3.85 ± 0.23 average shoots per explant) were achieved when in vitro grown nodal buds were cultured on MS medium containing optimized BAP along with 1 mg l
−1
naphthaleneacetic acid. Different solvent extracts of in vitro grown plantlets and wild plants were compared for acetylcholinesterase (AChE) inhibitory and antioxidant capacities. Highest AChE inhibitory activity was observed in ethanolic extract of wild plant biomass (IC
50
, 31.70 ± 0.16 µg ml
−1
) followed by ethanolic extract of in vitro plantlets (IC
50
, 44.17 ± 0.42 µg ml
−1
). On a whole, extracts of wild plant as well as in vitro plantlets exhibited the ability to scavenge DPPH and ABTS radicals, reduce ferric iron and protect DNA. These findings suggest that in vitro plantlets of
C. decussata
represent a promising alternative source for the botanicals ‘sankhapushpi’ for alleviating nervous ailments.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-015-0770-y</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antioxidants ; Cholinesterase ; Life Sciences ; Medicinal plants ; Plant biomass ; Plant extracts ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant propagation ; Plant Sciences ; Research Note</subject><ispartof>Plant cell, tissue and organ culture, 2015-08, Vol.122 (2), p.509-516</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2015). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c161y-bf951009b4631ac85f72447de71add1096a3fb112d5c146e78567cf72fce13723</citedby><cites>FETCH-LOGICAL-c161y-bf951009b4631ac85f72447de71add1096a3fb112d5c146e78567cf72fce13723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-015-0770-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-015-0770-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Gaikwad, Nitin Kundlikrao</creatorcontrib><creatorcontrib>Moon, Utkarsh Ravindra</creatorcontrib><creatorcontrib>Bhadoria, Pratapbhanu Singh</creatorcontrib><creatorcontrib>Mitra, Adinpunya</creatorcontrib><title>RETRACTED ARTICLE: In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Canscora decussata
Schult., popularly known as ‘shankhpushpi’, is a threatened medicinal plant. The whole plant is used in Ayurveda for curing insanity, epilepsy and nervous debility. Seasonal and limited availability along with the demand in herbal industries justify the need for developing in vitro plantlets for sustainable utilization of this plant. In the present study, an efficient protocol for in vitro propagation of
C. decussata
has been developed. Highest shoot induction was observed in the Murashige and Skoog (MS) basal medium supplemented with 0.2 mg l
−1
benzylaminopurine (BAP) when nodal explants were used. Enhanced shoot induction (83.34 %) and multiplication (3.85 ± 0.23 average shoots per explant) were achieved when in vitro grown nodal buds were cultured on MS medium containing optimized BAP along with 1 mg l
−1
naphthaleneacetic acid. Different solvent extracts of in vitro grown plantlets and wild plants were compared for acetylcholinesterase (AChE) inhibitory and antioxidant capacities. Highest AChE inhibitory activity was observed in ethanolic extract of wild plant biomass (IC
50
, 31.70 ± 0.16 µg ml
−1
) followed by ethanolic extract of in vitro plantlets (IC
50
, 44.17 ± 0.42 µg ml
−1
). On a whole, extracts of wild plant as well as in vitro plantlets exhibited the ability to scavenge DPPH and ABTS radicals, reduce ferric iron and protect DNA. These findings suggest that in vitro plantlets of
C. decussata
represent a promising alternative source for the botanicals ‘sankhapushpi’ for alleviating nervous ailments.</description><subject>Antioxidants</subject><subject>Cholinesterase</subject><subject>Life Sciences</subject><subject>Medicinal plants</subject><subject>Plant biomass</subject><subject>Plant extracts</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant propagation</subject><subject>Plant Sciences</subject><subject>Research Note</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kc1uEzEURi0EEqHwAOwssXax588z7KIhhUiRkEJYWze2J3E1sQdfp21ekOfC01RixepK1jnX9vcR8lHwW8G5_IxCFBVnXNSMS8nZ5RVZiFqWrOZV9ZosuGgka9paviXvEO85501ZiQX5s13ttst-t_pKl9vdut-svtC1pw8uxUCnGCY4QHLB0zDQHjzqEIEaq8-IkID-1MfzmG4peEN1OE0QM_1gKSBaxJP1aRbBJ8f0MYzOW0w2AtpnYz4PT87kSTVMoF1yFmfj0Y2GHSFmHq15ht3Lq9ghhkdPp3G27FOKoBO-J28GGNF-eJk35Nfdatd_Z5sf39b9csO0aMSF7YeuznF1-6opBei2HmRRVdJYKcAYwbsGymGfozS1FlVjZVs3Umdo0FaUsihvyKfr3hzN73P-jLoP5-jzlaoo6q7s2qqVmRJXSseAGO2gpuhOEC9KcDXXpa51qVyXmutSl-wUVwcz6w82_tv8f-kvdb2b6w</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Gaikwad, Nitin Kundlikrao</creator><creator>Moon, Utkarsh Ravindra</creator><creator>Bhadoria, Pratapbhanu Singh</creator><creator>Mitra, Adinpunya</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20150801</creationdate><title>RETRACTED ARTICLE: In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts</title><author>Gaikwad, Nitin Kundlikrao ; Moon, Utkarsh Ravindra ; Bhadoria, Pratapbhanu Singh ; Mitra, Adinpunya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c161y-bf951009b4631ac85f72447de71add1096a3fb112d5c146e78567cf72fce13723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antioxidants</topic><topic>Cholinesterase</topic><topic>Life Sciences</topic><topic>Medicinal plants</topic><topic>Plant biomass</topic><topic>Plant extracts</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant propagation</topic><topic>Plant Sciences</topic><topic>Research Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaikwad, Nitin Kundlikrao</creatorcontrib><creatorcontrib>Moon, Utkarsh Ravindra</creatorcontrib><creatorcontrib>Bhadoria, Pratapbhanu Singh</creatorcontrib><creatorcontrib>Mitra, Adinpunya</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaikwad, Nitin Kundlikrao</au><au>Moon, Utkarsh Ravindra</au><au>Bhadoria, Pratapbhanu Singh</au><au>Mitra, Adinpunya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RETRACTED ARTICLE: In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2015-08-01</date><risdate>2015</risdate><volume>122</volume><issue>2</issue><spage>509</spage><epage>516</epage><pages>509-516</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>Canscora decussata
Schult., popularly known as ‘shankhpushpi’, is a threatened medicinal plant. The whole plant is used in Ayurveda for curing insanity, epilepsy and nervous debility. Seasonal and limited availability along with the demand in herbal industries justify the need for developing in vitro plantlets for sustainable utilization of this plant. In the present study, an efficient protocol for in vitro propagation of
C. decussata
has been developed. Highest shoot induction was observed in the Murashige and Skoog (MS) basal medium supplemented with 0.2 mg l
−1
benzylaminopurine (BAP) when nodal explants were used. Enhanced shoot induction (83.34 %) and multiplication (3.85 ± 0.23 average shoots per explant) were achieved when in vitro grown nodal buds were cultured on MS medium containing optimized BAP along with 1 mg l
−1
naphthaleneacetic acid. Different solvent extracts of in vitro grown plantlets and wild plants were compared for acetylcholinesterase (AChE) inhibitory and antioxidant capacities. Highest AChE inhibitory activity was observed in ethanolic extract of wild plant biomass (IC
50
, 31.70 ± 0.16 µg ml
−1
) followed by ethanolic extract of in vitro plantlets (IC
50
, 44.17 ± 0.42 µg ml
−1
). On a whole, extracts of wild plant as well as in vitro plantlets exhibited the ability to scavenge DPPH and ABTS radicals, reduce ferric iron and protect DNA. These findings suggest that in vitro plantlets of
C. decussata
represent a promising alternative source for the botanicals ‘sankhapushpi’ for alleviating nervous ailments.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-015-0770-y</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2015-08, Vol.122 (2), p.509-516 |
| issn | 0167-6857 1573-5044 |
| language | eng |
| recordid | cdi_proquest_journals_2259398487 |
| source | SpringerNature Complete Journals |
| subjects | Antioxidants Cholinesterase Life Sciences Medicinal plants Plant biomass Plant extracts Plant Genetics and Genomics Plant Pathology Plant Physiology Plant propagation Plant Sciences Research Note |
| title | RETRACTED ARTICLE: In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts |
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