Somatic embryogenesis and plant regeneration of blackberry using the thin cell layer technique
The present study is the first to report somatic embryogenesis (SE) based on a plant regeneration protocol for blackberry. It uses transverse thin cell layer technique (tTCL). Two blackberry genotypes, ‘High prickle’ ( Rubus sanctus ) and ‘Low prickle’ ( Rubus hirtus ) were used as explants. The exp...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2017-08, Vol.130 (2), p.313-321 |
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| description | The present study is the first to report somatic embryogenesis (SE) based on a plant regeneration protocol for blackberry. It uses transverse thin cell layer technique (tTCL). Two blackberry genotypes, ‘High prickle’ (
Rubus sanctus
) and ‘Low prickle’ (
Rubus hirtus
) were used as explants. The explants were soaked in ascorbic and citric acids (60 mg l
−1
each) solution prior to culture on Murashige and Skoog (MS) medium containing 2.32 μM kinetin (KIN), 2.69 μM α-naphthaleneacetic acid (NAA) and 8.88 6-benzyladenine (BA). This not only reduced the phenolic compounds (in ‘High prickle’), but also produced friable and yellow-pale green calluses. The highest level of embryogenic callus initiation in both genotypes occurred in half strength MS medium containing 60 g l
−1
sucrose, 9.76 μM KIN and 7.99 μM BA. The MS medium fortified with 7.57 μM abscisic acid (ABA) and malt extract (700 mg l
−1
) or glutamine (400 mg l
−1
) encouraged the formation and development of embryos on calluses originating from dermal parts of ‘High prickle’ explants. Yasuda (YA) medium enrichd with 8.88 μM BA, 10.84 μM NAA and glycerol (2%) promoted embryo development and shoot regeneration on calluses originating from dermal parts of ‘High prickle’ and ‘Low prickle’ explants respectively. Germination of embryos and growth of normal plantlets occurred on half strength MS medium containing 4.88 μM BA, 2.02 μM gibberellic acid (GA
3
) and 0.05 μM NAA. Histological evaluations confirmed the successful occurrence of the different stages of embryogenesis. |
| doi_str_mv | 10.1007/s11240-017-1225-4 |
| format | Article |
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Rubus sanctus
) and ‘Low prickle’ (
Rubus hirtus
) were used as explants. The explants were soaked in ascorbic and citric acids (60 mg l
−1
each) solution prior to culture on Murashige and Skoog (MS) medium containing 2.32 μM kinetin (KIN), 2.69 μM α-naphthaleneacetic acid (NAA) and 8.88 6-benzyladenine (BA). This not only reduced the phenolic compounds (in ‘High prickle’), but also produced friable and yellow-pale green calluses. The highest level of embryogenic callus initiation in both genotypes occurred in half strength MS medium containing 60 g l
−1
sucrose, 9.76 μM KIN and 7.99 μM BA. The MS medium fortified with 7.57 μM abscisic acid (ABA) and malt extract (700 mg l
−1
) or glutamine (400 mg l
−1
) encouraged the formation and development of embryos on calluses originating from dermal parts of ‘High prickle’ explants. Yasuda (YA) medium enrichd with 8.88 μM BA, 10.84 μM NAA and glycerol (2%) promoted embryo development and shoot regeneration on calluses originating from dermal parts of ‘High prickle’ and ‘Low prickle’ explants respectively. Germination of embryos and growth of normal plantlets occurred on half strength MS medium containing 4.88 μM BA, 2.02 μM gibberellic acid (GA
3
) and 0.05 μM NAA. Histological evaluations confirmed the successful occurrence of the different stages of embryogenesis.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-017-1225-4</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Abscisic acid ; Benzyladenine ; Berries ; Biomedical and Life Sciences ; Callus ; Cell culture ; Embryonic growth stage ; Embryos ; Explants ; Friability ; Genotypes ; Germination ; Gibberellic acid ; Glutamine ; Glycerol ; Kinetin ; Life Sciences ; Malt ; Naphthaleneacetic acid ; Original Article ; Phenolic compounds ; Phenols ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Plantlets ; Regeneration ; Rubus ; Rubus hirtus ; Rubus sanctus ; Skin ; Somatic embryogenesis ; Sucrose ; Sugar ; Urban regeneration</subject><ispartof>Plant cell, tissue and organ culture, 2017-08, Vol.130 (2), p.313-321</ispartof><rights>Springer Science+Business Media Dordrecht 2017</rights><rights>Copyright Springer Science & Business Media 2017</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-a41b1faf441629f57896609f9c3a9f10c9060d0d7d9b7d7ff827ef436db8deda3</citedby><cites>FETCH-LOGICAL-c344t-a41b1faf441629f57896609f9c3a9f10c9060d0d7d9b7d7ff827ef436db8deda3</cites><orcidid>0000-0001-9705-9559</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-017-1225-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-017-1225-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Sabooni, Nasrin</creatorcontrib><creatorcontrib>Shekafandeh, Akhtar</creatorcontrib><title>Somatic embryogenesis and plant regeneration of blackberry using the thin cell layer technique</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>The present study is the first to report somatic embryogenesis (SE) based on a plant regeneration protocol for blackberry. It uses transverse thin cell layer technique (tTCL). Two blackberry genotypes, ‘High prickle’ (
Rubus sanctus
) and ‘Low prickle’ (
Rubus hirtus
) were used as explants. The explants were soaked in ascorbic and citric acids (60 mg l
−1
each) solution prior to culture on Murashige and Skoog (MS) medium containing 2.32 μM kinetin (KIN), 2.69 μM α-naphthaleneacetic acid (NAA) and 8.88 6-benzyladenine (BA). This not only reduced the phenolic compounds (in ‘High prickle’), but also produced friable and yellow-pale green calluses. The highest level of embryogenic callus initiation in both genotypes occurred in half strength MS medium containing 60 g l
−1
sucrose, 9.76 μM KIN and 7.99 μM BA. The MS medium fortified with 7.57 μM abscisic acid (ABA) and malt extract (700 mg l
−1
) or glutamine (400 mg l
−1
) encouraged the formation and development of embryos on calluses originating from dermal parts of ‘High prickle’ explants. Yasuda (YA) medium enrichd with 8.88 μM BA, 10.84 μM NAA and glycerol (2%) promoted embryo development and shoot regeneration on calluses originating from dermal parts of ‘High prickle’ and ‘Low prickle’ explants respectively. Germination of embryos and growth of normal plantlets occurred on half strength MS medium containing 4.88 μM BA, 2.02 μM gibberellic acid (GA
3
) and 0.05 μM NAA. Histological evaluations confirmed the successful occurrence of the different stages of embryogenesis.</description><subject>Abscisic acid</subject><subject>Benzyladenine</subject><subject>Berries</subject><subject>Biomedical and Life Sciences</subject><subject>Callus</subject><subject>Cell culture</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Explants</subject><subject>Friability</subject><subject>Genotypes</subject><subject>Germination</subject><subject>Gibberellic acid</subject><subject>Glutamine</subject><subject>Glycerol</subject><subject>Kinetin</subject><subject>Life Sciences</subject><subject>Malt</subject><subject>Naphthaleneacetic acid</subject><subject>Original Article</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plantlets</subject><subject>Regeneration</subject><subject>Rubus</subject><subject>Rubus hirtus</subject><subject>Rubus sanctus</subject><subject>Skin</subject><subject>Somatic embryogenesis</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Urban regeneration</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kD1PwzAQhi0EEqXwA9gsMQfubCeOR1TxJSExACuW49htSuoUOx3673EVBhYYTiednvc96SHkEuEaAeRNQmQCCkBZIGNlIY7IDEvJixKEOCYzwEoWVV3KU3KW0hoAKi5wRj5eh40ZO0vdpon7YemCS12iJrR025sw0ugOt5iZIdDB06Y39rNxMe7pLnVhSceVy9MFal3f097sXaSjs6vQfe3cOTnxpk_u4mfPyfv93dvisXh-eXha3D4XlgsxFkZgg954IbBiypeyVlUFyivLjfIIVkEFLbSyVY1spfc1k84LXrVN3brW8Dm5mnq3cchv06jXwy6G_FJnG4orXgL-R6FiAIIJpTKFE2XjkFJ0Xm9jtzFxrxH0QbaeZOssWx9ka5EzbMqkzIali7-a_wx9A-QVghg</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Sabooni, Nasrin</creator><creator>Shekafandeh, Akhtar</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><orcidid>https://orcid.org/0000-0001-9705-9559</orcidid></search><sort><creationdate>20170801</creationdate><title>Somatic embryogenesis and plant regeneration of blackberry using the thin cell layer technique</title><author>Sabooni, Nasrin ; Shekafandeh, Akhtar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-a41b1faf441629f57896609f9c3a9f10c9060d0d7d9b7d7ff827ef436db8deda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Abscisic acid</topic><topic>Benzyladenine</topic><topic>Berries</topic><topic>Biomedical and Life Sciences</topic><topic>Callus</topic><topic>Cell culture</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Explants</topic><topic>Friability</topic><topic>Genotypes</topic><topic>Germination</topic><topic>Gibberellic acid</topic><topic>Glutamine</topic><topic>Glycerol</topic><topic>Kinetin</topic><topic>Life Sciences</topic><topic>Malt</topic><topic>Naphthaleneacetic acid</topic><topic>Original Article</topic><topic>Phenolic compounds</topic><topic>Phenols</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plantlets</topic><topic>Regeneration</topic><topic>Rubus</topic><topic>Rubus hirtus</topic><topic>Rubus sanctus</topic><topic>Skin</topic><topic>Somatic embryogenesis</topic><topic>Sucrose</topic><topic>Sugar</topic><topic>Urban regeneration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabooni, Nasrin</creatorcontrib><creatorcontrib>Shekafandeh, Akhtar</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabooni, Nasrin</au><au>Shekafandeh, Akhtar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Somatic embryogenesis and plant regeneration of blackberry using the thin cell layer technique</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2017-08-01</date><risdate>2017</risdate><volume>130</volume><issue>2</issue><spage>313</spage><epage>321</epage><pages>313-321</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>The present study is the first to report somatic embryogenesis (SE) based on a plant regeneration protocol for blackberry. It uses transverse thin cell layer technique (tTCL). Two blackberry genotypes, ‘High prickle’ (
Rubus sanctus
) and ‘Low prickle’ (
Rubus hirtus
) were used as explants. The explants were soaked in ascorbic and citric acids (60 mg l
−1
each) solution prior to culture on Murashige and Skoog (MS) medium containing 2.32 μM kinetin (KIN), 2.69 μM α-naphthaleneacetic acid (NAA) and 8.88 6-benzyladenine (BA). This not only reduced the phenolic compounds (in ‘High prickle’), but also produced friable and yellow-pale green calluses. The highest level of embryogenic callus initiation in both genotypes occurred in half strength MS medium containing 60 g l
−1
sucrose, 9.76 μM KIN and 7.99 μM BA. The MS medium fortified with 7.57 μM abscisic acid (ABA) and malt extract (700 mg l
−1
) or glutamine (400 mg l
−1
) encouraged the formation and development of embryos on calluses originating from dermal parts of ‘High prickle’ explants. Yasuda (YA) medium enrichd with 8.88 μM BA, 10.84 μM NAA and glycerol (2%) promoted embryo development and shoot regeneration on calluses originating from dermal parts of ‘High prickle’ and ‘Low prickle’ explants respectively. Germination of embryos and growth of normal plantlets occurred on half strength MS medium containing 4.88 μM BA, 2.02 μM gibberellic acid (GA
3
) and 0.05 μM NAA. Histological evaluations confirmed the successful occurrence of the different stages of embryogenesis.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-017-1225-4</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-9705-9559</orcidid></addata></record> |
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| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2017-08, Vol.130 (2), p.313-321 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Abscisic acid Benzyladenine Berries Biomedical and Life Sciences Callus Cell culture Embryonic growth stage Embryos Explants Friability Genotypes Germination Gibberellic acid Glutamine Glycerol Kinetin Life Sciences Malt Naphthaleneacetic acid Original Article Phenolic compounds Phenols Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Plantlets Regeneration Rubus Rubus hirtus Rubus sanctus Skin Somatic embryogenesis Sucrose Sugar Urban regeneration |
| title | Somatic embryogenesis and plant regeneration of blackberry using the thin cell layer technique |
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