Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L
The effects of exogenous polyamines (PAs) on enhancement of somatic embryogenic calli was investigated in Momordica charantia L. in vitro. Induction of somatic embryogenesis (SE) in leaf explants of M. charantia after 21 days of culture in Murashige and Skoog (MS) medium was determined using scannin...
Gespeichert in:
| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2009-06, Vol.97 (3), p.303-311 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 311 |
|---|---|
| container_issue | 3 |
| container_start_page | 303 |
| container_title | Plant cell, tissue and organ culture |
| container_volume | 97 |
| creator | Paul, Ananya Mitter, Kalyan Raychaudhuri, Sarmistha Sen |
| description | The effects of exogenous polyamines (PAs) on enhancement of somatic embryogenic calli was investigated in Momordica charantia L. in vitro. Induction of somatic embryogenesis (SE) in leaf explants of M. charantia after 21 days of culture in Murashige and Skoog (MS) medium was determined using scanning electron microscopy. During induction of SE there were high titers of Putrescine (Put) as compared to Spermidine (Spd) and Spermine (Spm), a prerequisite for cell division. Addition of PAs to the embryogenic media resulted in an increase in fresh weights and number of somatic embryos of 21-day old embryogenic calli. Put at a concentration of 1 mM showed maximum increase in fresh weights of embryogenic calli (5 fold) and number of somatic embryos produced per 0.2 g of callus (2.5 fold). Moreover addition of PAs to the embryogenic media resulted in lowering of endogenous free PA level of 21-day old embryogenic calli. Thus, when the media was supplemented with exogenous PAs a positive correlation was found to exist between Somatic Embryogenesis enhancement and decrease in endogenous free PA levels. |
| doi_str_mv | 10.1007/s11240-009-9529-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2259348998</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2259348998</sourcerecordid><originalsourceid>FETCH-LOGICAL-c327t-dd0fb48212ad3af3fe64b7aed1d75483ba3cd01925ff38ee4fbd923698286af63</originalsourceid><addsrcrecordid>eNo9kMtKAzEUhoMoWKsP4MqAuBzNbSaTpZR6gUoX2nU4k0lqSmdSk6nQtzdDi6uz-L9z-xC6peSREiKfEqVMkIIQVaiSqUKeoQktJS9KIsQ5mhBayaKqS3mJrlLaEEIqLugELefOWTPg4PAubA_Q-d4mHHrse_zrhxhwCh0M3mDbNfEQ1jbnPo3xR-hCbL0BbL4hQj94wItrdOFgm-zNqU7R6mX-NXsrFsvX99nzojCcyaFoW-IaUTPKoOXguLOVaCTYlrayFDVvgJuWUMVK53htrXBNqxivVM3qClzFp-j-OHcXw8_epkFvwj72eaVmrFRc1ErVmaJHysSQUrRO76LvIB40JXr0po_edPamR29a5p6H02RIBrYuf2Z8-m9kVIyHlJm7O3IOgoZ1zMzqkxHKs2pOKyL5H3YCdsQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2259348998</pqid></control><display><type>article</type><title>Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L</title><source>SpringerNature Journals</source><creator>Paul, Ananya ; Mitter, Kalyan ; Raychaudhuri, Sarmistha Sen</creator><creatorcontrib>Paul, Ananya ; Mitter, Kalyan ; Raychaudhuri, Sarmistha Sen</creatorcontrib><description>The effects of exogenous polyamines (PAs) on enhancement of somatic embryogenic calli was investigated in Momordica charantia L. in vitro. Induction of somatic embryogenesis (SE) in leaf explants of M. charantia after 21 days of culture in Murashige and Skoog (MS) medium was determined using scanning electron microscopy. During induction of SE there were high titers of Putrescine (Put) as compared to Spermidine (Spd) and Spermine (Spm), a prerequisite for cell division. Addition of PAs to the embryogenic media resulted in an increase in fresh weights and number of somatic embryos of 21-day old embryogenic calli. Put at a concentration of 1 mM showed maximum increase in fresh weights of embryogenic calli (5 fold) and number of somatic embryos produced per 0.2 g of callus (2.5 fold). Moreover addition of PAs to the embryogenic media resulted in lowering of endogenous free PA level of 21-day old embryogenic calli. Thus, when the media was supplemented with exogenous PAs a positive correlation was found to exist between Somatic Embryogenesis enhancement and decrease in endogenous free PA levels.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-009-9529-7</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Heidelberg: Dordrecht : Springer Netherlands</publisher><subject>Biological and medical sciences ; Biotechnology ; Callus ; Cell culture ; Cell division ; culture media ; embryo culture ; Embryonic growth stage ; Embryos ; Explants ; Fundamental and applied biological sciences. Psychology ; leaves ; Momordica charantia ; Polyamines ; Putrescine ; Scanning electron microscopy ; Somatic embryogenesis ; somatic embryos ; Spermidine ; Spermine</subject><ispartof>Plant cell, tissue and organ culture, 2009-06, Vol.97 (3), p.303-311</ispartof><rights>2009 INIST-CNRS</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2009). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c327t-dd0fb48212ad3af3fe64b7aed1d75483ba3cd01925ff38ee4fbd923698286af63</citedby><cites>FETCH-LOGICAL-c327t-dd0fb48212ad3af3fe64b7aed1d75483ba3cd01925ff38ee4fbd923698286af63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21492365$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Paul, Ananya</creatorcontrib><creatorcontrib>Mitter, Kalyan</creatorcontrib><creatorcontrib>Raychaudhuri, Sarmistha Sen</creatorcontrib><title>Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L</title><title>Plant cell, tissue and organ culture</title><description>The effects of exogenous polyamines (PAs) on enhancement of somatic embryogenic calli was investigated in Momordica charantia L. in vitro. Induction of somatic embryogenesis (SE) in leaf explants of M. charantia after 21 days of culture in Murashige and Skoog (MS) medium was determined using scanning electron microscopy. During induction of SE there were high titers of Putrescine (Put) as compared to Spermidine (Spd) and Spermine (Spm), a prerequisite for cell division. Addition of PAs to the embryogenic media resulted in an increase in fresh weights and number of somatic embryos of 21-day old embryogenic calli. Put at a concentration of 1 mM showed maximum increase in fresh weights of embryogenic calli (5 fold) and number of somatic embryos produced per 0.2 g of callus (2.5 fold). Moreover addition of PAs to the embryogenic media resulted in lowering of endogenous free PA level of 21-day old embryogenic calli. Thus, when the media was supplemented with exogenous PAs a positive correlation was found to exist between Somatic Embryogenesis enhancement and decrease in endogenous free PA levels.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Callus</subject><subject>Cell culture</subject><subject>Cell division</subject><subject>culture media</subject><subject>embryo culture</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Explants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>leaves</subject><subject>Momordica charantia</subject><subject>Polyamines</subject><subject>Putrescine</subject><subject>Scanning electron microscopy</subject><subject>Somatic embryogenesis</subject><subject>somatic embryos</subject><subject>Spermidine</subject><subject>Spermine</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNo9kMtKAzEUhoMoWKsP4MqAuBzNbSaTpZR6gUoX2nU4k0lqSmdSk6nQtzdDi6uz-L9z-xC6peSREiKfEqVMkIIQVaiSqUKeoQktJS9KIsQ5mhBayaKqS3mJrlLaEEIqLugELefOWTPg4PAubA_Q-d4mHHrse_zrhxhwCh0M3mDbNfEQ1jbnPo3xR-hCbL0BbL4hQj94wItrdOFgm-zNqU7R6mX-NXsrFsvX99nzojCcyaFoW-IaUTPKoOXguLOVaCTYlrayFDVvgJuWUMVK53htrXBNqxivVM3qClzFp-j-OHcXw8_epkFvwj72eaVmrFRc1ErVmaJHysSQUrRO76LvIB40JXr0po_edPamR29a5p6H02RIBrYuf2Z8-m9kVIyHlJm7O3IOgoZ1zMzqkxHKs2pOKyL5H3YCdsQ</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Paul, Ananya</creator><creator>Mitter, Kalyan</creator><creator>Raychaudhuri, Sarmistha Sen</creator><general>Dordrecht : Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20090601</creationdate><title>Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L</title><author>Paul, Ananya ; Mitter, Kalyan ; Raychaudhuri, Sarmistha Sen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-dd0fb48212ad3af3fe64b7aed1d75483ba3cd01925ff38ee4fbd923698286af63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Callus</topic><topic>Cell culture</topic><topic>Cell division</topic><topic>culture media</topic><topic>embryo culture</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Explants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>leaves</topic><topic>Momordica charantia</topic><topic>Polyamines</topic><topic>Putrescine</topic><topic>Scanning electron microscopy</topic><topic>Somatic embryogenesis</topic><topic>somatic embryos</topic><topic>Spermidine</topic><topic>Spermine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paul, Ananya</creatorcontrib><creatorcontrib>Mitter, Kalyan</creatorcontrib><creatorcontrib>Raychaudhuri, Sarmistha Sen</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paul, Ananya</au><au>Mitter, Kalyan</au><au>Raychaudhuri, Sarmistha Sen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><date>2009-06-01</date><risdate>2009</risdate><volume>97</volume><issue>3</issue><spage>303</spage><epage>311</epage><pages>303-311</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>The effects of exogenous polyamines (PAs) on enhancement of somatic embryogenic calli was investigated in Momordica charantia L. in vitro. Induction of somatic embryogenesis (SE) in leaf explants of M. charantia after 21 days of culture in Murashige and Skoog (MS) medium was determined using scanning electron microscopy. During induction of SE there were high titers of Putrescine (Put) as compared to Spermidine (Spd) and Spermine (Spm), a prerequisite for cell division. Addition of PAs to the embryogenic media resulted in an increase in fresh weights and number of somatic embryos of 21-day old embryogenic calli. Put at a concentration of 1 mM showed maximum increase in fresh weights of embryogenic calli (5 fold) and number of somatic embryos produced per 0.2 g of callus (2.5 fold). Moreover addition of PAs to the embryogenic media resulted in lowering of endogenous free PA level of 21-day old embryogenic calli. Thus, when the media was supplemented with exogenous PAs a positive correlation was found to exist between Somatic Embryogenesis enhancement and decrease in endogenous free PA levels.</abstract><cop>Heidelberg</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s11240-009-9529-7</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2009-06, Vol.97 (3), p.303-311 |
| issn | 0167-6857 1573-5044 |
| language | eng |
| recordid | cdi_proquest_journals_2259348998 |
| source | SpringerNature Journals |
| subjects | Biological and medical sciences Biotechnology Callus Cell culture Cell division culture media embryo culture Embryonic growth stage Embryos Explants Fundamental and applied biological sciences. Psychology leaves Momordica charantia Polyamines Putrescine Scanning electron microscopy Somatic embryogenesis somatic embryos Spermidine Spermine |
| title | Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T14%3A40%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20polyamines%20on%20in%20vitro%20somatic%20embryogenesis%20in%20Momordica%20charantia%20L&rft.jtitle=Plant%20cell,%20tissue%20and%20organ%20culture&rft.au=Paul,%20Ananya&rft.date=2009-06-01&rft.volume=97&rft.issue=3&rft.spage=303&rft.epage=311&rft.pages=303-311&rft.issn=0167-6857&rft.eissn=1573-5044&rft.coden=PTCEDJ&rft_id=info:doi/10.1007/s11240-009-9529-7&rft_dat=%3Cproquest_cross%3E2259348998%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2259348998&rft_id=info:pmid/&rfr_iscdi=true |