Clinicopathological studies on the use of laser-activated adipose-derived stromal vascular fraction in treatment of streptozotocin-induced diabetes in rats
Diabetes mellitus (DM) is associated with severe progressive degenerative complications in many organs especially diabetic nephropathy (DN). Adipose-derived stromal vascular fraction (SVF) is easily obtained and has abundant viable stem cell number obviating extensive expansion in culture; thus, SVF...
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| Veröffentlicht in: | Comparative clinical pathology 2019-10, Vol.28 (5), p.1515-1526 |
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| creator | Sayed, Safaa Y. Salem, Shaymaa I. Abdallah, Ahmed N. Khalil, Ghada M. Mohammed, Faten F. |
| description | Diabetes mellitus (DM) is associated with severe progressive degenerative complications in many organs especially diabetic nephropathy (DN). Adipose-derived stromal vascular fraction (SVF) is easily obtained and has abundant viable stem cell number obviating extensive expansion in culture; thus, SVF utilization provides promising anticipation. Low-intensity laser irradiation (LILI) was found to strengthen the therapeutic effect of non-expanded SVF through enhancing viability, protein expression, and migration of stem cells. The current study aimed to demonstrate the effect of transplanted laser-activated SVF in streptozotocin (STZ)-induced diabetic rats. Forty-five male Sprague Dawley rats were used in this experiment and divided randomly into four groups: (I) control group, (II) diabetic untreated group, (III) and (IV) diabetic-treated groups in which laser-activated SVF transplantation performed as a single and multiple IP injections, respectively, (V) cell tracking group. DM was induced by a single intraperitoneal (IP) injection of STZ at a dose of 55 mg/kg. Rats received single and multiple IP SVF injections, at a dose of 1.5 × 10
6
nucleated cells/rat on the 7th day of DM induction and a second dose injected in group IV on the 21th day of DM induction (2-week interval). Insulin gene expression quantification; glycemic profile evaluation (blood glucose, C-peptide, and glycosylated hemoglobin); biochemical, histopathological, and immunohistochemical parameters; and microalbuminuria were assessed in all experimental groups. Both SVF-treated groups exhibited improvement in glycemic profile which was confirmed by the significant increase of insulin gene expression and C-peptide levels. Liver transaminases, lipid profile, and microalbuminuria were normalized while serum creatinine and urea concentrations were ameliorated in treated groups compared to diabetic untreated rats. In conclusion, laser-activated SVF transplantation in diabetic rats triggered improvement of the diabetic state and ameliorated some of its complications with regard to the early interference. The second SVF treatment showed more improvement regarding the blood glucose, C-peptide, histopathology, and immunohistochemical results of the pancreas in diabetic states. |
| doi_str_mv | 10.1007/s00580-019-03008-8 |
| format | Article |
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6
nucleated cells/rat on the 7th day of DM induction and a second dose injected in group IV on the 21th day of DM induction (2-week interval). Insulin gene expression quantification; glycemic profile evaluation (blood glucose, C-peptide, and glycosylated hemoglobin); biochemical, histopathological, and immunohistochemical parameters; and microalbuminuria were assessed in all experimental groups. Both SVF-treated groups exhibited improvement in glycemic profile which was confirmed by the significant increase of insulin gene expression and C-peptide levels. Liver transaminases, lipid profile, and microalbuminuria were normalized while serum creatinine and urea concentrations were ameliorated in treated groups compared to diabetic untreated rats. In conclusion, laser-activated SVF transplantation in diabetic rats triggered improvement of the diabetic state and ameliorated some of its complications with regard to the early interference. The second SVF treatment showed more improvement regarding the blood glucose, C-peptide, histopathology, and immunohistochemical results of the pancreas in diabetic states.</description><identifier>ISSN: 1618-5641</identifier><identifier>EISSN: 1618-565X</identifier><identifier>DOI: 10.1007/s00580-019-03008-8</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Blood glucose ; Cell culture ; Cell migration ; Cell number ; Creatinine ; Diabetes ; Diabetes mellitus ; Diabetic nephropathy ; Gene expression ; Hematology ; Hemoglobin ; Insulin ; Lasers ; Medicine ; Medicine & Public Health ; Nephropathy ; Oncology ; Original Article ; Pancreas ; Pathology ; Peptides ; Stem cell transplantation ; Stem cells ; Streptozocin ; Urea</subject><ispartof>Comparative clinical pathology, 2019-10, Vol.28 (5), p.1515-1526</ispartof><rights>Springer-Verlag London Ltd., part of Springer Nature 2019</rights><rights>Comparative Clinical Pathology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2348-82e6ae9004b2b65466e164355a90c7a1f220aa6f567905d6cdada2437bc7c9b83</citedby><cites>FETCH-LOGICAL-c2348-82e6ae9004b2b65466e164355a90c7a1f220aa6f567905d6cdada2437bc7c9b83</cites><orcidid>0000-0002-1299-5469</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00580-019-03008-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00580-019-03008-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Sayed, Safaa Y.</creatorcontrib><creatorcontrib>Salem, Shaymaa I.</creatorcontrib><creatorcontrib>Abdallah, Ahmed N.</creatorcontrib><creatorcontrib>Khalil, Ghada M.</creatorcontrib><creatorcontrib>Mohammed, Faten F.</creatorcontrib><title>Clinicopathological studies on the use of laser-activated adipose-derived stromal vascular fraction in treatment of streptozotocin-induced diabetes in rats</title><title>Comparative clinical pathology</title><addtitle>Comp Clin Pathol</addtitle><description>Diabetes mellitus (DM) is associated with severe progressive degenerative complications in many organs especially diabetic nephropathy (DN). Adipose-derived stromal vascular fraction (SVF) is easily obtained and has abundant viable stem cell number obviating extensive expansion in culture; thus, SVF utilization provides promising anticipation. Low-intensity laser irradiation (LILI) was found to strengthen the therapeutic effect of non-expanded SVF through enhancing viability, protein expression, and migration of stem cells. The current study aimed to demonstrate the effect of transplanted laser-activated SVF in streptozotocin (STZ)-induced diabetic rats. Forty-five male Sprague Dawley rats were used in this experiment and divided randomly into four groups: (I) control group, (II) diabetic untreated group, (III) and (IV) diabetic-treated groups in which laser-activated SVF transplantation performed as a single and multiple IP injections, respectively, (V) cell tracking group. DM was induced by a single intraperitoneal (IP) injection of STZ at a dose of 55 mg/kg. Rats received single and multiple IP SVF injections, at a dose of 1.5 × 10
6
nucleated cells/rat on the 7th day of DM induction and a second dose injected in group IV on the 21th day of DM induction (2-week interval). Insulin gene expression quantification; glycemic profile evaluation (blood glucose, C-peptide, and glycosylated hemoglobin); biochemical, histopathological, and immunohistochemical parameters; and microalbuminuria were assessed in all experimental groups. Both SVF-treated groups exhibited improvement in glycemic profile which was confirmed by the significant increase of insulin gene expression and C-peptide levels. Liver transaminases, lipid profile, and microalbuminuria were normalized while serum creatinine and urea concentrations were ameliorated in treated groups compared to diabetic untreated rats. In conclusion, laser-activated SVF transplantation in diabetic rats triggered improvement of the diabetic state and ameliorated some of its complications with regard to the early interference. The second SVF treatment showed more improvement regarding the blood glucose, C-peptide, histopathology, and immunohistochemical results of the pancreas in diabetic states.</description><subject>Blood glucose</subject><subject>Cell culture</subject><subject>Cell migration</subject><subject>Cell number</subject><subject>Creatinine</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetic nephropathy</subject><subject>Gene expression</subject><subject>Hematology</subject><subject>Hemoglobin</subject><subject>Insulin</subject><subject>Lasers</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nephropathy</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Pancreas</subject><subject>Pathology</subject><subject>Peptides</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Streptozocin</subject><subject>Urea</subject><issn>1618-5641</issn><issn>1618-565X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kcFK5TAUhoso6Kgv4KrgOuNJ2qTtUi6OCsJsFNyF0-RUI71NTdILzqv4suZ6xdlJFkng-7_D4S-KMw6_OUBzEQFkCwx4x6ACaFm7VxxxxVsmlXzc_37X_LD4FeMLAJdtVR0V76vRTc74GdOzH_2TMziWMS3WUSz9VKZnKpdIpR_KESMFhia5DSayJVo3-0jMUnCb_I8p-HVObzCaZcRQDmELZ4nLnkCY1jSlrSmTNCf_zydv3MTcZBeTBdZhTynPzXzAFE-KgwHHSKdf93Hx8OfqfnXD7v5e364u75gRVZ1XFaSQOoC6F72StVLEVV1JiR2YBvkgBCCqQaqmA2mVsWhR1FXTm8Z0fVsdF-c77xz860Ix6Re_hCmP1ELIts5HNZkSO8oEH2OgQc_BrTG8aQ56W4LelaBzCfqzBL1VV7tQzPD0ROG_-ofUB6jWjiE</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Sayed, Safaa Y.</creator><creator>Salem, Shaymaa I.</creator><creator>Abdallah, Ahmed N.</creator><creator>Khalil, Ghada M.</creator><creator>Mohammed, Faten F.</creator><general>Springer London</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0002-1299-5469</orcidid></search><sort><creationdate>20191001</creationdate><title>Clinicopathological studies on the use of laser-activated adipose-derived stromal vascular fraction in treatment of streptozotocin-induced diabetes in rats</title><author>Sayed, Safaa Y. ; Salem, Shaymaa I. ; Abdallah, Ahmed N. ; Khalil, Ghada M. ; Mohammed, Faten F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2348-82e6ae9004b2b65466e164355a90c7a1f220aa6f567905d6cdada2437bc7c9b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Blood glucose</topic><topic>Cell culture</topic><topic>Cell migration</topic><topic>Cell number</topic><topic>Creatinine</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetic nephropathy</topic><topic>Gene expression</topic><topic>Hematology</topic><topic>Hemoglobin</topic><topic>Insulin</topic><topic>Lasers</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nephropathy</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Pancreas</topic><topic>Pathology</topic><topic>Peptides</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Streptozocin</topic><topic>Urea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sayed, Safaa Y.</creatorcontrib><creatorcontrib>Salem, Shaymaa I.</creatorcontrib><creatorcontrib>Abdallah, Ahmed N.</creatorcontrib><creatorcontrib>Khalil, Ghada M.</creatorcontrib><creatorcontrib>Mohammed, Faten F.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Comparative clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sayed, Safaa Y.</au><au>Salem, Shaymaa I.</au><au>Abdallah, Ahmed N.</au><au>Khalil, Ghada M.</au><au>Mohammed, Faten F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinicopathological studies on the use of laser-activated adipose-derived stromal vascular fraction in treatment of streptozotocin-induced diabetes in rats</atitle><jtitle>Comparative clinical pathology</jtitle><stitle>Comp Clin Pathol</stitle><date>2019-10-01</date><risdate>2019</risdate><volume>28</volume><issue>5</issue><spage>1515</spage><epage>1526</epage><pages>1515-1526</pages><issn>1618-5641</issn><eissn>1618-565X</eissn><abstract>Diabetes mellitus (DM) is associated with severe progressive degenerative complications in many organs especially diabetic nephropathy (DN). Adipose-derived stromal vascular fraction (SVF) is easily obtained and has abundant viable stem cell number obviating extensive expansion in culture; thus, SVF utilization provides promising anticipation. Low-intensity laser irradiation (LILI) was found to strengthen the therapeutic effect of non-expanded SVF through enhancing viability, protein expression, and migration of stem cells. The current study aimed to demonstrate the effect of transplanted laser-activated SVF in streptozotocin (STZ)-induced diabetic rats. Forty-five male Sprague Dawley rats were used in this experiment and divided randomly into four groups: (I) control group, (II) diabetic untreated group, (III) and (IV) diabetic-treated groups in which laser-activated SVF transplantation performed as a single and multiple IP injections, respectively, (V) cell tracking group. DM was induced by a single intraperitoneal (IP) injection of STZ at a dose of 55 mg/kg. Rats received single and multiple IP SVF injections, at a dose of 1.5 × 10
6
nucleated cells/rat on the 7th day of DM induction and a second dose injected in group IV on the 21th day of DM induction (2-week interval). Insulin gene expression quantification; glycemic profile evaluation (blood glucose, C-peptide, and glycosylated hemoglobin); biochemical, histopathological, and immunohistochemical parameters; and microalbuminuria were assessed in all experimental groups. Both SVF-treated groups exhibited improvement in glycemic profile which was confirmed by the significant increase of insulin gene expression and C-peptide levels. Liver transaminases, lipid profile, and microalbuminuria were normalized while serum creatinine and urea concentrations were ameliorated in treated groups compared to diabetic untreated rats. In conclusion, laser-activated SVF transplantation in diabetic rats triggered improvement of the diabetic state and ameliorated some of its complications with regard to the early interference. The second SVF treatment showed more improvement regarding the blood glucose, C-peptide, histopathology, and immunohistochemical results of the pancreas in diabetic states.</abstract><cop>London</cop><pub>Springer London</pub><doi>10.1007/s00580-019-03008-8</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-1299-5469</orcidid></addata></record> |
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| subjects | Blood glucose Cell culture Cell migration Cell number Creatinine Diabetes Diabetes mellitus Diabetic nephropathy Gene expression Hematology Hemoglobin Insulin Lasers Medicine Medicine & Public Health Nephropathy Oncology Original Article Pancreas Pathology Peptides Stem cell transplantation Stem cells Streptozocin Urea |
| title | Clinicopathological studies on the use of laser-activated adipose-derived stromal vascular fraction in treatment of streptozotocin-induced diabetes in rats |
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