Genetic analyses of causal genes of albinism (white fruiting body) in Grifola frondosa

Genetic analyses of causal genes of albinism (white fruiting body) in Grifola frondosa

The tyrosinase 2 gene ( tyr2 ) from two compatible strains of Grifola frondosa , the albino-type monokaryon strain IM-WM1-25 and the wild-type monokaryon strain IM-BM11-P21, were amplified and characterized (designated tyr2 −Δ25 and tyr2 + , respectively). A single base deletion in the coding region...

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Veröffentlicht in:Journal of wood science 2019-12, Vol.65 (1), p.1-9, Article 32
Hauptverfasser: Kawaguchi, Nobuhisa, Hayashi, Mirai, Chen, Fu-Chia, Shimomura, Norihiro, Yamaguchi, Takeshi, Aimi, Tadanori
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Albinism
Biomedical and Life Sciences
Characterization and Evaluation of Materials
Deletion
Life Sciences
Materials Science
Mushrooms
Mutation
Original Article
Polymerase chain reaction
Proteins
Strain analysis
Tyrosinase
Wood Science & Technology
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container_issue 1
container_start_page 1
container_title Journal of wood science
container_volume 65
creator Kawaguchi, Nobuhisa
Hayashi, Mirai
Chen, Fu-Chia
Shimomura, Norihiro
Yamaguchi, Takeshi
Aimi, Tadanori
description The tyrosinase 2 gene ( tyr2 ) from two compatible strains of Grifola frondosa , the albino-type monokaryon strain IM-WM1-25 and the wild-type monokaryon strain IM-BM11-P21, were amplified and characterized (designated tyr2 −Δ25 and tyr2 + , respectively). A single base deletion in the coding region of tyr2 −Δ25 from IM-WM1-25 was discovered, and this mutation is predicted to cause a frame-shift in translation, yielding inactive protein tyrosinase protein 2 (TYR2). Polymerase chain reaction (PCR) primer pairs were designed to detect normal tyr2 + and mutant tyr2 −Δ25 , and then the tyr2 genotype of F1 progenies, which was obtained from basidiospore isolation of IM-BM11-P21 × IM-WM1-25 ( tyr +  ×  tyr2 −Δ25 ) strain, was analyzed. Back-crossing (F1 progenies × IM-WM1-25) was performed and fruiting body colors of the crossed strains were analyzed. The fruiting bodies of all crossed strains were white and beige, and the corresponding genotypes were tyr2 −Δ25  ×  tyr2 −Δ25 and tyr +  ×  tyr2 −Δ25 . These results suggest that the causal gene of the albino mutation is tyr2 and this study provides a new strategy for the breeding of albino mushrooms belonging to G. frondosa .
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A single base deletion in the coding region of tyr2 −Δ25 from IM-WM1-25 was discovered, and this mutation is predicted to cause a frame-shift in translation, yielding inactive protein tyrosinase protein 2 (TYR2). Polymerase chain reaction (PCR) primer pairs were designed to detect normal tyr2 + and mutant tyr2 −Δ25 , and then the tyr2 genotype of F1 progenies, which was obtained from basidiospore isolation of IM-BM11-P21 × IM-WM1-25 ( tyr +  ×  tyr2 −Δ25 ) strain, was analyzed. Back-crossing (F1 progenies × IM-WM1-25) was performed and fruiting body colors of the crossed strains were analyzed. The fruiting bodies of all crossed strains were white and beige, and the corresponding genotypes were tyr2 −Δ25  ×  tyr2 −Δ25 and tyr +  ×  tyr2 −Δ25 . 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A single base deletion in the coding region of tyr2 −Δ25 from IM-WM1-25 was discovered, and this mutation is predicted to cause a frame-shift in translation, yielding inactive protein tyrosinase protein 2 (TYR2). Polymerase chain reaction (PCR) primer pairs were designed to detect normal tyr2 + and mutant tyr2 −Δ25 , and then the tyr2 genotype of F1 progenies, which was obtained from basidiospore isolation of IM-BM11-P21 × IM-WM1-25 ( tyr +  ×  tyr2 −Δ25 ) strain, was analyzed. Back-crossing (F1 progenies × IM-WM1-25) was performed and fruiting body colors of the crossed strains were analyzed. The fruiting bodies of all crossed strains were white and beige, and the corresponding genotypes were tyr2 −Δ25  ×  tyr2 −Δ25 and tyr +  ×  tyr2 −Δ25 . 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A single base deletion in the coding region of tyr2 −Δ25 from IM-WM1-25 was discovered, and this mutation is predicted to cause a frame-shift in translation, yielding inactive protein tyrosinase protein 2 (TYR2). Polymerase chain reaction (PCR) primer pairs were designed to detect normal tyr2 + and mutant tyr2 −Δ25 , and then the tyr2 genotype of F1 progenies, which was obtained from basidiospore isolation of IM-BM11-P21 × IM-WM1-25 ( tyr +  ×  tyr2 −Δ25 ) strain, was analyzed. Back-crossing (F1 progenies × IM-WM1-25) was performed and fruiting body colors of the crossed strains were analyzed. The fruiting bodies of all crossed strains were white and beige, and the corresponding genotypes were tyr2 −Δ25  ×  tyr2 −Δ25 and tyr +  ×  tyr2 −Δ25 . 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subjects Albinism
Biomedical and Life Sciences
Characterization and Evaluation of Materials
Deletion
Life Sciences
Materials Science
Mushrooms
Mutation
Original Article
Polymerase chain reaction
Proteins
Strain analysis
Tyrosinase
Wood Science & Technology
title Genetic analyses of causal genes of albinism (white fruiting body) in Grifola frondosa
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