Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus
The aim of this study was to develop a cold-adapted influenza A (H7N9) strain-based vector vaccine expressing epitopes of conserved lipoprotein of Streptococcus agalactiae . Recombinant hemagglutinin (HA) genes of live attenuated influenza vaccine (LAIV) strain A/Anhui/1/2013(H7N9) with insertions o...
Gespeichert in:
| Veröffentlicht in: | Molecular genetics, microbiology and virology microbiology and virology, 2019, Vol.34 (1), p.25-34 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 34 |
|---|---|
| container_issue | 1 |
| container_start_page | 25 |
| container_title | Molecular genetics, microbiology and virology |
| container_volume | 34 |
| creator | Smolonogina, T. A. Isakova-Sivak, I. N. Kotomina, T. S. Evsina, A. S. Stepanova, E. A. Prokopenko, P. I. Leontieva, G. F. Suvorov, A. N. Rudenko, L. G. |
| description | The aim of this study was to develop a cold-adapted influenza A (H7N9) strain-based vector vaccine expressing epitopes of conserved lipoprotein of
Streptococcus agalactiae
. Recombinant hemagglutinin (HA) genes of live attenuated influenza vaccine (LAIV) strain A/Anhui/1/2013(H7N9) with insertions of group B Streptococcus (GBS) surface antigen ScaAB gene parts of different length in-frame between signal peptide and HA1 were constructed by means of reverse genetics. Viral vector vaccine H7-ScaAB-85 expressing the shortest (85 amino acids) polypeptide from lipoprotein ScaAB linked to HA via a flexible linker was genetically stable, replicated in embryonated chicken eggs and MDCK cells at the same level as cold-adapted influenza A (H7N9) strain. An increase of ScaAB polypeptide insertion length up to 141 amino acids resulted in a decrease of the viral replication level in both susceptible systems and intense mutagenesis of hybrid HA-gene of recombinant influenza A virus in the gene region encoding foreign bacterial lipoprotein. These findings suggest that H7-ScaAB-85 is a promising vaccine candidate, which should be further assessed using a mouse model. |
| doi_str_mv | 10.3103/S0891416819010087 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2254569811</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2254569811</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-1122ff12e22ca34c4bf78fc6c2a3dfc3713d6a5f92cd196948e717e8918102bf3</originalsourceid><addsrcrecordid>eNp1kLFOwzAQhi0EEqXwAGyWmAM-O3Gcsa0gVKrEUKjYItexS6pgB9sZ4OlJFCQGxHTDfd9_uh-hayC3DAi72xJRQApcQEGAEJGfoBkULE1ECq-naDauk3F_ji5COBKSccr4DHWlttrL2DiLncES76RSjdVYHmRjQ8Sld32Hl3gbve6iU04p2eK1NVpNksXxTeOlDE2YElaurZNFLbuo6xFse22_JF7gXeP7cInOjGyDvvqZc_TycP-8ekw2T-V6tdgkigGPCQClxgDVlCrJUpXuTS6M4opKVhvFcmA1l5kpqKqh4EUqdA65Hr4UQOjesDm6mXI77z56HWJ1dL23w8mK0izNeCEABgomSnkXgtem6nzzLv1nBaQai63-FDs4dHLCwNqD9r_J_0vfbqJ5Xw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2254569811</pqid></control><display><type>article</type><title>Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus</title><source>SpringerLink Journals - AutoHoldings</source><creator>Smolonogina, T. A. ; Isakova-Sivak, I. N. ; Kotomina, T. S. ; Evsina, A. S. ; Stepanova, E. A. ; Prokopenko, P. I. ; Leontieva, G. F. ; Suvorov, A. N. ; Rudenko, L. G.</creator><creatorcontrib>Smolonogina, T. A. ; Isakova-Sivak, I. N. ; Kotomina, T. S. ; Evsina, A. S. ; Stepanova, E. A. ; Prokopenko, P. I. ; Leontieva, G. F. ; Suvorov, A. N. ; Rudenko, L. G.</creatorcontrib><description>The aim of this study was to develop a cold-adapted influenza A (H7N9) strain-based vector vaccine expressing epitopes of conserved lipoprotein of
Streptococcus agalactiae
. Recombinant hemagglutinin (HA) genes of live attenuated influenza vaccine (LAIV) strain A/Anhui/1/2013(H7N9) with insertions of group B Streptococcus (GBS) surface antigen ScaAB gene parts of different length in-frame between signal peptide and HA1 were constructed by means of reverse genetics. Viral vector vaccine H7-ScaAB-85 expressing the shortest (85 amino acids) polypeptide from lipoprotein ScaAB linked to HA via a flexible linker was genetically stable, replicated in embryonated chicken eggs and MDCK cells at the same level as cold-adapted influenza A (H7N9) strain. An increase of ScaAB polypeptide insertion length up to 141 amino acids resulted in a decrease of the viral replication level in both susceptible systems and intense mutagenesis of hybrid HA-gene of recombinant influenza A virus in the gene region encoding foreign bacterial lipoprotein. These findings suggest that H7-ScaAB-85 is a promising vaccine candidate, which should be further assessed using a mouse model.</description><identifier>ISSN: 0891-4168</identifier><identifier>EISSN: 1934-841X</identifier><identifier>DOI: 10.3103/S0891416819010087</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Amino acids ; Biomedical and Life Sciences ; Eggs ; Epitopes ; Experimental Works ; Hemagglutinins ; Influenza ; Influenza A ; Insertion ; Life Sciences ; Lipoproteins ; Microbiology ; Molecular Medicine ; Mutagenesis ; Polypeptides ; Streptococcus ; Vaccines</subject><ispartof>Molecular genetics, microbiology and virology, 2019, Vol.34 (1), p.25-34</ispartof><rights>Allerton Press, Inc. 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-1122ff12e22ca34c4bf78fc6c2a3dfc3713d6a5f92cd196948e717e8918102bf3</citedby><cites>FETCH-LOGICAL-c316t-1122ff12e22ca34c4bf78fc6c2a3dfc3713d6a5f92cd196948e717e8918102bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3103/S0891416819010087$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3103/S0891416819010087$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Smolonogina, T. A.</creatorcontrib><creatorcontrib>Isakova-Sivak, I. N.</creatorcontrib><creatorcontrib>Kotomina, T. S.</creatorcontrib><creatorcontrib>Evsina, A. S.</creatorcontrib><creatorcontrib>Stepanova, E. A.</creatorcontrib><creatorcontrib>Prokopenko, P. I.</creatorcontrib><creatorcontrib>Leontieva, G. F.</creatorcontrib><creatorcontrib>Suvorov, A. N.</creatorcontrib><creatorcontrib>Rudenko, L. G.</creatorcontrib><title>Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus</title><title>Molecular genetics, microbiology and virology</title><addtitle>Mol. Genet. Microbiol. Virol</addtitle><description>The aim of this study was to develop a cold-adapted influenza A (H7N9) strain-based vector vaccine expressing epitopes of conserved lipoprotein of
Streptococcus agalactiae
. Recombinant hemagglutinin (HA) genes of live attenuated influenza vaccine (LAIV) strain A/Anhui/1/2013(H7N9) with insertions of group B Streptococcus (GBS) surface antigen ScaAB gene parts of different length in-frame between signal peptide and HA1 were constructed by means of reverse genetics. Viral vector vaccine H7-ScaAB-85 expressing the shortest (85 amino acids) polypeptide from lipoprotein ScaAB linked to HA via a flexible linker was genetically stable, replicated in embryonated chicken eggs and MDCK cells at the same level as cold-adapted influenza A (H7N9) strain. An increase of ScaAB polypeptide insertion length up to 141 amino acids resulted in a decrease of the viral replication level in both susceptible systems and intense mutagenesis of hybrid HA-gene of recombinant influenza A virus in the gene region encoding foreign bacterial lipoprotein. These findings suggest that H7-ScaAB-85 is a promising vaccine candidate, which should be further assessed using a mouse model.</description><subject>Amino acids</subject><subject>Biomedical and Life Sciences</subject><subject>Eggs</subject><subject>Epitopes</subject><subject>Experimental Works</subject><subject>Hemagglutinins</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Insertion</subject><subject>Life Sciences</subject><subject>Lipoproteins</subject><subject>Microbiology</subject><subject>Molecular Medicine</subject><subject>Mutagenesis</subject><subject>Polypeptides</subject><subject>Streptococcus</subject><subject>Vaccines</subject><issn>0891-4168</issn><issn>1934-841X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kLFOwzAQhi0EEqXwAGyWmAM-O3Gcsa0gVKrEUKjYItexS6pgB9sZ4OlJFCQGxHTDfd9_uh-hayC3DAi72xJRQApcQEGAEJGfoBkULE1ECq-naDauk3F_ji5COBKSccr4DHWlttrL2DiLncES76RSjdVYHmRjQ8Sld32Hl3gbve6iU04p2eK1NVpNksXxTeOlDE2YElaurZNFLbuo6xFse22_JF7gXeP7cInOjGyDvvqZc_TycP-8ekw2T-V6tdgkigGPCQClxgDVlCrJUpXuTS6M4opKVhvFcmA1l5kpqKqh4EUqdA65Hr4UQOjesDm6mXI77z56HWJ1dL23w8mK0izNeCEABgomSnkXgtem6nzzLv1nBaQai63-FDs4dHLCwNqD9r_J_0vfbqJ5Xw</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>Smolonogina, T. A.</creator><creator>Isakova-Sivak, I. N.</creator><creator>Kotomina, T. S.</creator><creator>Evsina, A. S.</creator><creator>Stepanova, E. A.</creator><creator>Prokopenko, P. I.</creator><creator>Leontieva, G. F.</creator><creator>Suvorov, A. N.</creator><creator>Rudenko, L. G.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2019</creationdate><title>Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus</title><author>Smolonogina, T. A. ; Isakova-Sivak, I. N. ; Kotomina, T. S. ; Evsina, A. S. ; Stepanova, E. A. ; Prokopenko, P. I. ; Leontieva, G. F. ; Suvorov, A. N. ; Rudenko, L. G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-1122ff12e22ca34c4bf78fc6c2a3dfc3713d6a5f92cd196948e717e8918102bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Amino acids</topic><topic>Biomedical and Life Sciences</topic><topic>Eggs</topic><topic>Epitopes</topic><topic>Experimental Works</topic><topic>Hemagglutinins</topic><topic>Influenza</topic><topic>Influenza A</topic><topic>Insertion</topic><topic>Life Sciences</topic><topic>Lipoproteins</topic><topic>Microbiology</topic><topic>Molecular Medicine</topic><topic>Mutagenesis</topic><topic>Polypeptides</topic><topic>Streptococcus</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smolonogina, T. A.</creatorcontrib><creatorcontrib>Isakova-Sivak, I. N.</creatorcontrib><creatorcontrib>Kotomina, T. S.</creatorcontrib><creatorcontrib>Evsina, A. S.</creatorcontrib><creatorcontrib>Stepanova, E. A.</creatorcontrib><creatorcontrib>Prokopenko, P. I.</creatorcontrib><creatorcontrib>Leontieva, G. F.</creatorcontrib><creatorcontrib>Suvorov, A. N.</creatorcontrib><creatorcontrib>Rudenko, L. G.</creatorcontrib><collection>CrossRef</collection><jtitle>Molecular genetics, microbiology and virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smolonogina, T. A.</au><au>Isakova-Sivak, I. N.</au><au>Kotomina, T. S.</au><au>Evsina, A. S.</au><au>Stepanova, E. A.</au><au>Prokopenko, P. I.</au><au>Leontieva, G. F.</au><au>Suvorov, A. N.</au><au>Rudenko, L. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus</atitle><jtitle>Molecular genetics, microbiology and virology</jtitle><stitle>Mol. Genet. Microbiol. Virol</stitle><date>2019</date><risdate>2019</risdate><volume>34</volume><issue>1</issue><spage>25</spage><epage>34</epage><pages>25-34</pages><issn>0891-4168</issn><eissn>1934-841X</eissn><abstract>The aim of this study was to develop a cold-adapted influenza A (H7N9) strain-based vector vaccine expressing epitopes of conserved lipoprotein of
Streptococcus agalactiae
. Recombinant hemagglutinin (HA) genes of live attenuated influenza vaccine (LAIV) strain A/Anhui/1/2013(H7N9) with insertions of group B Streptococcus (GBS) surface antigen ScaAB gene parts of different length in-frame between signal peptide and HA1 were constructed by means of reverse genetics. Viral vector vaccine H7-ScaAB-85 expressing the shortest (85 amino acids) polypeptide from lipoprotein ScaAB linked to HA via a flexible linker was genetically stable, replicated in embryonated chicken eggs and MDCK cells at the same level as cold-adapted influenza A (H7N9) strain. An increase of ScaAB polypeptide insertion length up to 141 amino acids resulted in a decrease of the viral replication level in both susceptible systems and intense mutagenesis of hybrid HA-gene of recombinant influenza A virus in the gene region encoding foreign bacterial lipoprotein. These findings suggest that H7-ScaAB-85 is a promising vaccine candidate, which should be further assessed using a mouse model.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.3103/S0891416819010087</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0891-4168 |
| ispartof | Molecular genetics, microbiology and virology, 2019, Vol.34 (1), p.25-34 |
| issn | 0891-4168 1934-841X |
| language | eng |
| recordid | cdi_proquest_journals_2254569811 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Amino acids Biomedical and Life Sciences Eggs Epitopes Experimental Works Hemagglutinins Influenza Influenza A Insertion Life Sciences Lipoproteins Microbiology Molecular Medicine Mutagenesis Polypeptides Streptococcus Vaccines |
| title | Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T00%3A29%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Generation%20of%20a%20Vaccine%20against%20Group%20B%20Streptococcal%20Infection%20on%20the%20Basis%20of%20a%20Cold-Adapted%20Influenza%20A%20Virus&rft.jtitle=Molecular%20genetics,%20microbiology%20and%20virology&rft.au=Smolonogina,%20T.%20A.&rft.date=2019&rft.volume=34&rft.issue=1&rft.spage=25&rft.epage=34&rft.pages=25-34&rft.issn=0891-4168&rft.eissn=1934-841X&rft_id=info:doi/10.3103/S0891416819010087&rft_dat=%3Cproquest_cross%3E2254569811%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2254569811&rft_id=info:pmid/&rfr_iscdi=true |