MicroRNA 196B Regulates HOXA5, HOXB6 and GLTP Expression Levels in Colorectal Cancer Cells
MiRNAs are non-coding RNAs that play important roles in the pathogenesis of human diseases by regulating target gene expression in specific cells or tissues. Previously we identified colorectal cancer (CRC) associated MIR196B , which was specifically up-regulated in CRC cells and tissue. We also ide...
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| Veröffentlicht in: | Pathology oncology research 2019-07, Vol.25 (3), p.953-959 |
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| description | MiRNAs are non-coding RNAs that play important roles in the pathogenesis of human diseases by regulating target gene expression in specific cells or tissues. Previously we identified colorectal cancer (CRC) associated
MIR196B
, which was specifically up-regulated in CRC cells and tissue. We also identified 18 putative
MIR196B
target genes by comparing the mRNAs down-regulated in
MIR196B
-overexpressed cells with
MIR196B
target genes predicted by public bioinformatics tools. In this study, we verified the association between
MIR196B
and three genes,
HOXA5
,
HOXB6
and
GLTP
.
HOXA5
,
HOXB6
and
GLTP
transcripts were directly down-regulated by
MIR196B
. The mRNA and proteins levels of HOXA5, HOXB6 and GLTP were also down-regulated in CRC cells by the up-regulated
MIR196B
. GLTP protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues. These results suggest that
HOXA5
,
HOXB6
, and
GLTP
were direct target genes of
MIR196B
in CRC cells, and that the up-regulated MIR196B in CRC tissue regulates the expression levels of HOXA5, HOXB6, and GLTP during colorectal carcinogenesis. |
| doi_str_mv | 10.1007/s12253-018-0399-3 |
| format | Article |
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MIR196B
, which was specifically up-regulated in CRC cells and tissue. We also identified 18 putative
MIR196B
target genes by comparing the mRNAs down-regulated in
MIR196B
-overexpressed cells with
MIR196B
target genes predicted by public bioinformatics tools. In this study, we verified the association between
MIR196B
and three genes,
HOXA5
,
HOXB6
and
GLTP
.
HOXA5
,
HOXB6
and
GLTP
transcripts were directly down-regulated by
MIR196B
. The mRNA and proteins levels of HOXA5, HOXB6 and GLTP were also down-regulated in CRC cells by the up-regulated
MIR196B
. GLTP protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues. These results suggest that
HOXA5
,
HOXB6
, and
GLTP
were direct target genes of
MIR196B
in CRC cells, and that the up-regulated MIR196B in CRC tissue regulates the expression levels of HOXA5, HOXB6, and GLTP during colorectal carcinogenesis.</description><identifier>ISSN: 1219-4956</identifier><identifier>EISSN: 1532-2807</identifier><identifier>DOI: 10.1007/s12253-018-0399-3</identifier><identifier>PMID: 29532406</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Aged ; Bioinformatics ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Carcinogenesis ; Carrier Proteins - genetics ; Cell Line, Tumor ; Cell Movement - genetics ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - genetics ; Down-Regulation - genetics ; Gene expression ; Gene Expression Regulation, Neoplastic - genetics ; Genes ; Homeodomain Proteins - genetics ; Humans ; Immunology ; MicroRNAs - genetics ; Middle Aged ; miRNA ; Oncology ; Original Article ; Pathology ; Proteins ; RNA, Messenger - genetics ; Tissues ; Up-Regulation - genetics</subject><ispartof>Pathology oncology research, 2019-07, Vol.25 (3), p.953-959</ispartof><rights>Arányi Lajos Foundation 2018</rights><rights>Pathology & Oncology Research is a copyright of Springer, (2018). All Rights Reserved.</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-b59d3fcb06f6d427e44597626c57a7856eb75e4cea529e72151f081d5d620b1c3</citedby><cites>FETCH-LOGICAL-c400t-b59d3fcb06f6d427e44597626c57a7856eb75e4cea529e72151f081d5d620b1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12253-018-0399-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12253-018-0399-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29532406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mo, Ji-Su</creatorcontrib><creatorcontrib>Park, Young-Ran</creatorcontrib><creatorcontrib>Chae, Soo-Cheon</creatorcontrib><title>MicroRNA 196B Regulates HOXA5, HOXB6 and GLTP Expression Levels in Colorectal Cancer Cells</title><title>Pathology oncology research</title><addtitle>Pathol. Oncol. Res</addtitle><addtitle>Pathol Oncol Res</addtitle><description>MiRNAs are non-coding RNAs that play important roles in the pathogenesis of human diseases by regulating target gene expression in specific cells or tissues. Previously we identified colorectal cancer (CRC) associated
MIR196B
, which was specifically up-regulated in CRC cells and tissue. We also identified 18 putative
MIR196B
target genes by comparing the mRNAs down-regulated in
MIR196B
-overexpressed cells with
MIR196B
target genes predicted by public bioinformatics tools. In this study, we verified the association between
MIR196B
and three genes,
HOXA5
,
HOXB6
and
GLTP
.
HOXA5
,
HOXB6
and
GLTP
transcripts were directly down-regulated by
MIR196B
. The mRNA and proteins levels of HOXA5, HOXB6 and GLTP were also down-regulated in CRC cells by the up-regulated
MIR196B
. GLTP protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues. These results suggest that
HOXA5
,
HOXB6
, and
GLTP
were direct target genes of
MIR196B
in CRC cells, and that the up-regulated MIR196B in CRC tissue regulates the expression levels of HOXA5, HOXB6, and GLTP during colorectal carcinogenesis.</description><subject>Aged</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Carcinogenesis</subject><subject>Carrier Proteins - genetics</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Down-Regulation - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>Genes</subject><subject>Homeodomain Proteins - genetics</subject><subject>Humans</subject><subject>Immunology</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Pathology</subject><subject>Proteins</subject><subject>RNA, Messenger - genetics</subject><subject>Tissues</subject><subject>Up-Regulation - genetics</subject><issn>1219-4956</issn><issn>1532-2807</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kE1Lw0AQhhdRrFZ_gBdZ8Gp0P7Kb7rENtRWilVJBvCz5mJSUNKm7iei_d0OqnvQ0A_O878CD0AUlN5SQ4NZSxgT3CB15hCvl8QN0QgVnHhuR4NDtjCrPV0IO0Km1G-IyUsljNGDKUT6RJ-j1oUhNvXwcY6rkBC9h3ZZxAxbPFy9jcd2NicRxleFZtHrC04-dAWuLusIRvENpcVHhsC5rA2kTlziMqxQMDqEs7Rk6yuPSwvl-DtHz3XQVzr1oMbsPx5GX-oQ0XiJUxvM0ITKXmc8C8H2hAslkKoI4GAkJSSDATyEWTEHAqKA5GdFMZJKRhKZ8iK763p2p31qwjd7UrancS93pUdwpIf9ShIqOk8JRtKecFGsN5Hpnim1sPjUlunOue-faOdedc81d5nLf3CZbyH4S35IdwHrAulO1BvP7-u_WLzthh0E</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Mo, Ji-Su</creator><creator>Park, Young-Ran</creator><creator>Chae, Soo-Cheon</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20190701</creationdate><title>MicroRNA 196B Regulates HOXA5, HOXB6 and GLTP Expression Levels in Colorectal Cancer Cells</title><author>Mo, Ji-Su ; Park, Young-Ran ; Chae, Soo-Cheon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-b59d3fcb06f6d427e44597626c57a7856eb75e4cea529e72151f081d5d620b1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aged</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Carcinogenesis</topic><topic>Carrier Proteins - genetics</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Down-Regulation - genetics</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic - genetics</topic><topic>Genes</topic><topic>Homeodomain Proteins - genetics</topic><topic>Humans</topic><topic>Immunology</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>miRNA</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Pathology</topic><topic>Proteins</topic><topic>RNA, Messenger - genetics</topic><topic>Tissues</topic><topic>Up-Regulation - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mo, Ji-Su</creatorcontrib><creatorcontrib>Park, Young-Ran</creatorcontrib><creatorcontrib>Chae, Soo-Cheon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Pathology oncology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mo, Ji-Su</au><au>Park, Young-Ran</au><au>Chae, Soo-Cheon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA 196B Regulates HOXA5, HOXB6 and GLTP Expression Levels in Colorectal Cancer Cells</atitle><jtitle>Pathology oncology research</jtitle><stitle>Pathol. Oncol. Res</stitle><addtitle>Pathol Oncol Res</addtitle><date>2019-07-01</date><risdate>2019</risdate><volume>25</volume><issue>3</issue><spage>953</spage><epage>959</epage><pages>953-959</pages><issn>1219-4956</issn><eissn>1532-2807</eissn><abstract>MiRNAs are non-coding RNAs that play important roles in the pathogenesis of human diseases by regulating target gene expression in specific cells or tissues. Previously we identified colorectal cancer (CRC) associated
MIR196B
, which was specifically up-regulated in CRC cells and tissue. We also identified 18 putative
MIR196B
target genes by comparing the mRNAs down-regulated in
MIR196B
-overexpressed cells with
MIR196B
target genes predicted by public bioinformatics tools. In this study, we verified the association between
MIR196B
and three genes,
HOXA5
,
HOXB6
and
GLTP
.
HOXA5
,
HOXB6
and
GLTP
transcripts were directly down-regulated by
MIR196B
. The mRNA and proteins levels of HOXA5, HOXB6 and GLTP were also down-regulated in CRC cells by the up-regulated
MIR196B
. GLTP protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues. These results suggest that
HOXA5
,
HOXB6
, and
GLTP
were direct target genes of
MIR196B
in CRC cells, and that the up-regulated MIR196B in CRC tissue regulates the expression levels of HOXA5, HOXB6, and GLTP during colorectal carcinogenesis.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29532406</pmid><doi>10.1007/s12253-018-0399-3</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1219-4956 |
| ispartof | Pathology oncology research, 2019-07, Vol.25 (3), p.953-959 |
| issn | 1219-4956 1532-2807 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Aged Bioinformatics Biomedical and Life Sciences Biomedicine Cancer Research Carcinogenesis Carrier Proteins - genetics Cell Line, Tumor Cell Movement - genetics Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - genetics Down-Regulation - genetics Gene expression Gene Expression Regulation, Neoplastic - genetics Genes Homeodomain Proteins - genetics Humans Immunology MicroRNAs - genetics Middle Aged miRNA Oncology Original Article Pathology Proteins RNA, Messenger - genetics Tissues Up-Regulation - genetics |
| title | MicroRNA 196B Regulates HOXA5, HOXB6 and GLTP Expression Levels in Colorectal Cancer Cells |
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