Effect of bioformulations on the biocontrol efficacy, microbial viability and storage stability of a consortium of biocontrol agents against Fusarium wilt of banana
Aims This study sought to investigate the effect of bioformulation on the biocontrol efficacy, microbial viability and storage stability of a consortium of Pseudomonas aeruginosa DRB1 and Trichoderma harzianum CBF2 against Foc Tropical Race 4 (Foc‐TR4). Materials and Results Four bioformulations con...
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| Veröffentlicht in: | Journal of applied microbiology 2019-08, Vol.127 (2), p.544-555 |
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| creator | Wong, C.K.F. Saidi, N.B. Vadamalai, G. Teh, C.Y. Zulperi, D. |
| description | Aims
This study sought to investigate the effect of bioformulation on the biocontrol efficacy, microbial viability and storage stability of a consortium of Pseudomonas aeruginosa DRB1 and Trichoderma harzianum CBF2 against Foc Tropical Race 4 (Foc‐TR4).
Materials and Results
Four bioformulations consisting of dry (pesta granules, talc powder and alginate beads) and liquid formulations were evaluated for their ability to control Foc‐TR4, sustain microbial populations after application and maintain microbial stability during storage. All tested bioformulations reduced disease severity (DS) by more than 43·00% with pesta granules producing the highest reduction in DS by 66·67% and the lowest area under the disease progress curve value (468·75) in a glasshouse trial. Microbial populations of DRB1 and CBF2 were abundant in the rhizosphere, rhizoplane and within the roots of bananas after pesta granules application as compared to talc powder, alginate beads and liquid formulations 84 days after inoculation (DAI). The stability of both microbial populations after 180 days of storage at 4°C was the greatest in the pesta granule formulation.
Conclusion
The pesta granule formulation was a suitable carrier of biological control agents (BCA) without compromising biocontrol efficacy, microbial population and storage stability as compared to other bioformulations used in this study.
Significance and Impact of the Study
Pesta granules could be utilized to formulate BCA consortia into biofertilizers. This formulation could be further investigated for possible applications under agricultural field settings. |
| doi_str_mv | 10.1111/jam.14310 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2252989426</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2252989426</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3550-a35cedfa5be986b10af4ed89b36a9096755ed6c921c8c85bbee11addaa719a253</originalsourceid><addsrcrecordid>eNp1kctOAyEUhonR2HpZ-AKGxJWJY4Epc1k2jdfUuNH15MCA0swMFRhN38cHlTqtO2FxyMmX7wA_QmeUXNO4Jktor-k0pWQPjWma8YRlOdv_PU8TTnI2QkfeLwmhKeHZIRpFNM85zcfo-0ZrJQO2GgtjtXVt30AwtvPYdji8q01b2i4422CltZEg11e4NdJZYaDBnwaEaUxYY-hq7IN18KZi3XWjGHAUeOuC6dvtoJ0xsl3wsYDpfMC3vQe3ob5MM9wJurhP0IGGxqvTbT1Gr7c3L_P7ZPF89zCfLRKZck4SSLlUtQYuVFlkghLQU1UXpUgzKEmZ5ZyrOpMlo7KQBRdCKUqhrgFyWgLj6TG6GLwrZz965UO1tL3r4siKMc7KopyyLFKXAxW_wHundLVypgW3riipNnlUMY_qN4_Inm-NvWhV_UfuAojAZADii9X6f1P1OHsalD-bS5jC</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2252989426</pqid></control><display><type>article</type><title>Effect of bioformulations on the biocontrol efficacy, microbial viability and storage stability of a consortium of biocontrol agents against Fusarium wilt of banana</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Wong, C.K.F. ; Saidi, N.B. ; Vadamalai, G. ; Teh, C.Y. ; Zulperi, D.</creator><creatorcontrib>Wong, C.K.F. ; Saidi, N.B. ; Vadamalai, G. ; Teh, C.Y. ; Zulperi, D.</creatorcontrib><description>Aims
This study sought to investigate the effect of bioformulation on the biocontrol efficacy, microbial viability and storage stability of a consortium of Pseudomonas aeruginosa DRB1 and Trichoderma harzianum CBF2 against Foc Tropical Race 4 (Foc‐TR4).
Materials and Results
Four bioformulations consisting of dry (pesta granules, talc powder and alginate beads) and liquid formulations were evaluated for their ability to control Foc‐TR4, sustain microbial populations after application and maintain microbial stability during storage. All tested bioformulations reduced disease severity (DS) by more than 43·00% with pesta granules producing the highest reduction in DS by 66·67% and the lowest area under the disease progress curve value (468·75) in a glasshouse trial. Microbial populations of DRB1 and CBF2 were abundant in the rhizosphere, rhizoplane and within the roots of bananas after pesta granules application as compared to talc powder, alginate beads and liquid formulations 84 days after inoculation (DAI). The stability of both microbial populations after 180 days of storage at 4°C was the greatest in the pesta granule formulation.
Conclusion
The pesta granule formulation was a suitable carrier of biological control agents (BCA) without compromising biocontrol efficacy, microbial population and storage stability as compared to other bioformulations used in this study.
Significance and Impact of the Study
Pesta granules could be utilized to formulate BCA consortia into biofertilizers. This formulation could be further investigated for possible applications under agricultural field settings.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14310</identifier><identifier>PMID: 31077517</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Agricultural land ; Alginates ; Alginic acid ; banana ; Bananas ; BCA consortium ; Beads ; biocontrol efficacy ; Biofertilizers ; bioformulation ; Biological control ; Biological Control Agents ; Consortia ; Control stability ; Drb1 protein ; Effectiveness ; Fertilizers ; Formulations ; Fungi ; Fusarium ; Fusarium wilt ; Granular materials ; Greenhouses ; Inoculation ; Microbial Consortia ; microbial population ; Microbial Viability ; Microorganisms ; Musa - microbiology ; pesta granules ; Plant Diseases - microbiology ; Plant Diseases - prevention & control ; Plant Roots - microbiology ; Populations ; Powder ; Pseudomonas aeruginosa ; Rhizoplane ; Rhizosphere ; Shelf life ; Storage stability ; Talc ; Trichoderma ; Trichoderma harzianum ; Viability ; Wilt</subject><ispartof>Journal of applied microbiology, 2019-08, Vol.127 (2), p.544-555</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2019 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3550-a35cedfa5be986b10af4ed89b36a9096755ed6c921c8c85bbee11addaa719a253</citedby><cites>FETCH-LOGICAL-c3550-a35cedfa5be986b10af4ed89b36a9096755ed6c921c8c85bbee11addaa719a253</cites><orcidid>0000-0002-6468-657X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14310$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14310$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31077517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wong, C.K.F.</creatorcontrib><creatorcontrib>Saidi, N.B.</creatorcontrib><creatorcontrib>Vadamalai, G.</creatorcontrib><creatorcontrib>Teh, C.Y.</creatorcontrib><creatorcontrib>Zulperi, D.</creatorcontrib><title>Effect of bioformulations on the biocontrol efficacy, microbial viability and storage stability of a consortium of biocontrol agents against Fusarium wilt of banana</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
This study sought to investigate the effect of bioformulation on the biocontrol efficacy, microbial viability and storage stability of a consortium of Pseudomonas aeruginosa DRB1 and Trichoderma harzianum CBF2 against Foc Tropical Race 4 (Foc‐TR4).
Materials and Results
Four bioformulations consisting of dry (pesta granules, talc powder and alginate beads) and liquid formulations were evaluated for their ability to control Foc‐TR4, sustain microbial populations after application and maintain microbial stability during storage. All tested bioformulations reduced disease severity (DS) by more than 43·00% with pesta granules producing the highest reduction in DS by 66·67% and the lowest area under the disease progress curve value (468·75) in a glasshouse trial. Microbial populations of DRB1 and CBF2 were abundant in the rhizosphere, rhizoplane and within the roots of bananas after pesta granules application as compared to talc powder, alginate beads and liquid formulations 84 days after inoculation (DAI). The stability of both microbial populations after 180 days of storage at 4°C was the greatest in the pesta granule formulation.
Conclusion
The pesta granule formulation was a suitable carrier of biological control agents (BCA) without compromising biocontrol efficacy, microbial population and storage stability as compared to other bioformulations used in this study.
Significance and Impact of the Study
Pesta granules could be utilized to formulate BCA consortia into biofertilizers. This formulation could be further investigated for possible applications under agricultural field settings.</description><subject>Agricultural land</subject><subject>Alginates</subject><subject>Alginic acid</subject><subject>banana</subject><subject>Bananas</subject><subject>BCA consortium</subject><subject>Beads</subject><subject>biocontrol efficacy</subject><subject>Biofertilizers</subject><subject>bioformulation</subject><subject>Biological control</subject><subject>Biological Control Agents</subject><subject>Consortia</subject><subject>Control stability</subject><subject>Drb1 protein</subject><subject>Effectiveness</subject><subject>Fertilizers</subject><subject>Formulations</subject><subject>Fungi</subject><subject>Fusarium</subject><subject>Fusarium wilt</subject><subject>Granular materials</subject><subject>Greenhouses</subject><subject>Inoculation</subject><subject>Microbial Consortia</subject><subject>microbial population</subject><subject>Microbial Viability</subject><subject>Microorganisms</subject><subject>Musa - microbiology</subject><subject>pesta granules</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Diseases - prevention & control</subject><subject>Plant Roots - microbiology</subject><subject>Populations</subject><subject>Powder</subject><subject>Pseudomonas aeruginosa</subject><subject>Rhizoplane</subject><subject>Rhizosphere</subject><subject>Shelf life</subject><subject>Storage stability</subject><subject>Talc</subject><subject>Trichoderma</subject><subject>Trichoderma harzianum</subject><subject>Viability</subject><subject>Wilt</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctOAyEUhonR2HpZ-AKGxJWJY4Epc1k2jdfUuNH15MCA0swMFRhN38cHlTqtO2FxyMmX7wA_QmeUXNO4Jktor-k0pWQPjWma8YRlOdv_PU8TTnI2QkfeLwmhKeHZIRpFNM85zcfo-0ZrJQO2GgtjtXVt30AwtvPYdji8q01b2i4422CltZEg11e4NdJZYaDBnwaEaUxYY-hq7IN18KZi3XWjGHAUeOuC6dvtoJ0xsl3wsYDpfMC3vQe3ob5MM9wJurhP0IGGxqvTbT1Gr7c3L_P7ZPF89zCfLRKZck4SSLlUtQYuVFlkghLQU1UXpUgzKEmZ5ZyrOpMlo7KQBRdCKUqhrgFyWgLj6TG6GLwrZz965UO1tL3r4siKMc7KopyyLFKXAxW_wHundLVypgW3riipNnlUMY_qN4_Inm-NvWhV_UfuAojAZADii9X6f1P1OHsalD-bS5jC</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Wong, C.K.F.</creator><creator>Saidi, N.B.</creator><creator>Vadamalai, G.</creator><creator>Teh, C.Y.</creator><creator>Zulperi, D.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-6468-657X</orcidid></search><sort><creationdate>201908</creationdate><title>Effect of bioformulations on the biocontrol efficacy, microbial viability and storage stability of a consortium of biocontrol agents against Fusarium wilt of banana</title><author>Wong, C.K.F. ; Saidi, N.B. ; Vadamalai, G. ; Teh, C.Y. ; Zulperi, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3550-a35cedfa5be986b10af4ed89b36a9096755ed6c921c8c85bbee11addaa719a253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agricultural land</topic><topic>Alginates</topic><topic>Alginic acid</topic><topic>banana</topic><topic>Bananas</topic><topic>BCA consortium</topic><topic>Beads</topic><topic>biocontrol efficacy</topic><topic>Biofertilizers</topic><topic>bioformulation</topic><topic>Biological control</topic><topic>Biological Control Agents</topic><topic>Consortia</topic><topic>Control stability</topic><topic>Drb1 protein</topic><topic>Effectiveness</topic><topic>Fertilizers</topic><topic>Formulations</topic><topic>Fungi</topic><topic>Fusarium</topic><topic>Fusarium wilt</topic><topic>Granular materials</topic><topic>Greenhouses</topic><topic>Inoculation</topic><topic>Microbial Consortia</topic><topic>microbial population</topic><topic>Microbial Viability</topic><topic>Microorganisms</topic><topic>Musa - microbiology</topic><topic>pesta granules</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Diseases - prevention & control</topic><topic>Plant Roots - microbiology</topic><topic>Populations</topic><topic>Powder</topic><topic>Pseudomonas aeruginosa</topic><topic>Rhizoplane</topic><topic>Rhizosphere</topic><topic>Shelf life</topic><topic>Storage stability</topic><topic>Talc</topic><topic>Trichoderma</topic><topic>Trichoderma harzianum</topic><topic>Viability</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wong, C.K.F.</creatorcontrib><creatorcontrib>Saidi, N.B.</creatorcontrib><creatorcontrib>Vadamalai, G.</creatorcontrib><creatorcontrib>Teh, C.Y.</creatorcontrib><creatorcontrib>Zulperi, D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wong, C.K.F.</au><au>Saidi, N.B.</au><au>Vadamalai, G.</au><au>Teh, C.Y.</au><au>Zulperi, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of bioformulations on the biocontrol efficacy, microbial viability and storage stability of a consortium of biocontrol agents against Fusarium wilt of banana</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2019-08</date><risdate>2019</risdate><volume>127</volume><issue>2</issue><spage>544</spage><epage>555</epage><pages>544-555</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
This study sought to investigate the effect of bioformulation on the biocontrol efficacy, microbial viability and storage stability of a consortium of Pseudomonas aeruginosa DRB1 and Trichoderma harzianum CBF2 against Foc Tropical Race 4 (Foc‐TR4).
Materials and Results
Four bioformulations consisting of dry (pesta granules, talc powder and alginate beads) and liquid formulations were evaluated for their ability to control Foc‐TR4, sustain microbial populations after application and maintain microbial stability during storage. All tested bioformulations reduced disease severity (DS) by more than 43·00% with pesta granules producing the highest reduction in DS by 66·67% and the lowest area under the disease progress curve value (468·75) in a glasshouse trial. Microbial populations of DRB1 and CBF2 were abundant in the rhizosphere, rhizoplane and within the roots of bananas after pesta granules application as compared to talc powder, alginate beads and liquid formulations 84 days after inoculation (DAI). The stability of both microbial populations after 180 days of storage at 4°C was the greatest in the pesta granule formulation.
Conclusion
The pesta granule formulation was a suitable carrier of biological control agents (BCA) without compromising biocontrol efficacy, microbial population and storage stability as compared to other bioformulations used in this study.
Significance and Impact of the Study
Pesta granules could be utilized to formulate BCA consortia into biofertilizers. This formulation could be further investigated for possible applications under agricultural field settings.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31077517</pmid><doi>10.1111/jam.14310</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-6468-657X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Agricultural land Alginates Alginic acid banana Bananas BCA consortium Beads biocontrol efficacy Biofertilizers bioformulation Biological control Biological Control Agents Consortia Control stability Drb1 protein Effectiveness Fertilizers Formulations Fungi Fusarium Fusarium wilt Granular materials Greenhouses Inoculation Microbial Consortia microbial population Microbial Viability Microorganisms Musa - microbiology pesta granules Plant Diseases - microbiology Plant Diseases - prevention & control Plant Roots - microbiology Populations Powder Pseudomonas aeruginosa Rhizoplane Rhizosphere Shelf life Storage stability Talc Trichoderma Trichoderma harzianum Viability Wilt |
| title | Effect of bioformulations on the biocontrol efficacy, microbial viability and storage stability of a consortium of biocontrol agents against Fusarium wilt of banana |
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