An electrochemiluminescence biosensor for the detection of soybean agglutinin based on carboxylated graphitic carbon nitride as luminophore
As an important glycoprotein of the lectin family, soybean agglutinin (SBA) is an anti-nutritional factor with considerable toxic and side effects and plays a significant role in tumor analysis. In order to achieve the sensitive detection of SBA, a sandwich-structured electrochemiluminescence (ECL)...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2019-09, Vol.411 (23), p.6049-6056 |
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| creator | Zhang, Cong Hu, Fangxin Zhang, Han Chen, Shihong Yuan, Ruo |
| description | As an important glycoprotein of the lectin family, soybean agglutinin (SBA) is an anti-nutritional factor with considerable toxic and side effects and plays a significant role in tumor analysis. In order to achieve the sensitive detection of SBA, a sandwich-structured electrochemiluminescence (ECL) biosensor was constructed using carboxylated carbon nitride (C-g-C
3
N
4
) as luminophore and D-galactosamine (galM) as a recognition element. A glassy carbon electrode (GCE) was modified with Au nanoparticles (Au NPs) for capturing the galM via Au-N bond, and further capturing the target SBA by specific recognition between galM and SBA. In the presence of SBA, the composite C-g-C
3
N
4
-galM was immobilized onto the electrode. With the increase in the concentration of SBA, the ECL signal from C-g-C
3
N
4
increased, thus achieving a signal-on detection of SBA. The linear range of the biosensor was 1.0 ng/mL~10 μg/mL and detection limit for SBA was as low as 0.33 ng/mL. In this construction strategy, C-g-C
3
N
4
not only acted as an excellent signal probe, but also as an immobilization matrix to easily achieve a high loading of the small molecule recognition element galM. This strategy provides a simple alternative SBA detection platform.
Graphical abstract |
| doi_str_mv | 10.1007/s00216-019-01986-w |
| format | Article |
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3
N
4
) as luminophore and D-galactosamine (galM) as a recognition element. A glassy carbon electrode (GCE) was modified with Au nanoparticles (Au NPs) for capturing the galM via Au-N bond, and further capturing the target SBA by specific recognition between galM and SBA. In the presence of SBA, the composite C-g-C
3
N
4
-galM was immobilized onto the electrode. With the increase in the concentration of SBA, the ECL signal from C-g-C
3
N
4
increased, thus achieving a signal-on detection of SBA. The linear range of the biosensor was 1.0 ng/mL~10 μg/mL and detection limit for SBA was as low as 0.33 ng/mL. In this construction strategy, C-g-C
3
N
4
not only acted as an excellent signal probe, but also as an immobilization matrix to easily achieve a high loading of the small molecule recognition element galM. This strategy provides a simple alternative SBA detection platform.
Graphical abstract</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-019-01986-w</identifier><identifier>PMID: 31280477</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Antinutrition factors ; Biochemistry ; Biosensors ; Carbon ; Carbon nitride ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; D-Galactosamine ; Detectors ; Electrochemiluminescence ; Electrodes ; Food Science ; Glassy carbon ; Glycoproteins ; Gold ; Immobilization ; Laboratory Medicine ; Lectins ; Monitoring/Environmental Analysis ; Nanoparticles ; Nitrides ; Research Paper ; Side effects ; Soybean ; Soybeans ; Target recognition</subject><ispartof>Analytical and bioanalytical chemistry, 2019-09, Vol.411 (23), p.6049-6056</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-419c9e228ed5e7f3b52ba21063abdd75b6a975d64f63a83bbe4c8b36f02dc82a3</citedby><cites>FETCH-LOGICAL-c480t-419c9e228ed5e7f3b52ba21063abdd75b6a975d64f63a83bbe4c8b36f02dc82a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-019-01986-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-019-01986-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31280477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Cong</creatorcontrib><creatorcontrib>Hu, Fangxin</creatorcontrib><creatorcontrib>Zhang, Han</creatorcontrib><creatorcontrib>Chen, Shihong</creatorcontrib><creatorcontrib>Yuan, Ruo</creatorcontrib><title>An electrochemiluminescence biosensor for the detection of soybean agglutinin based on carboxylated graphitic carbon nitride as luminophore</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>As an important glycoprotein of the lectin family, soybean agglutinin (SBA) is an anti-nutritional factor with considerable toxic and side effects and plays a significant role in tumor analysis. In order to achieve the sensitive detection of SBA, a sandwich-structured electrochemiluminescence (ECL) biosensor was constructed using carboxylated carbon nitride (C-g-C
3
N
4
) as luminophore and D-galactosamine (galM) as a recognition element. A glassy carbon electrode (GCE) was modified with Au nanoparticles (Au NPs) for capturing the galM via Au-N bond, and further capturing the target SBA by specific recognition between galM and SBA. In the presence of SBA, the composite C-g-C
3
N
4
-galM was immobilized onto the electrode. With the increase in the concentration of SBA, the ECL signal from C-g-C
3
N
4
increased, thus achieving a signal-on detection of SBA. The linear range of the biosensor was 1.0 ng/mL~10 μg/mL and detection limit for SBA was as low as 0.33 ng/mL. In this construction strategy, C-g-C
3
N
4
not only acted as an excellent signal probe, but also as an immobilization matrix to easily achieve a high loading of the small molecule recognition element galM. This strategy provides a simple alternative SBA detection platform.
Graphical abstract</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Antinutrition factors</subject><subject>Biochemistry</subject><subject>Biosensors</subject><subject>Carbon</subject><subject>Carbon nitride</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>D-Galactosamine</subject><subject>Detectors</subject><subject>Electrochemiluminescence</subject><subject>Electrodes</subject><subject>Food Science</subject><subject>Glassy carbon</subject><subject>Glycoproteins</subject><subject>Gold</subject><subject>Immobilization</subject><subject>Laboratory Medicine</subject><subject>Lectins</subject><subject>Monitoring/Environmental Analysis</subject><subject>Nanoparticles</subject><subject>Nitrides</subject><subject>Research Paper</subject><subject>Side effects</subject><subject>Soybean</subject><subject>Soybeans</subject><subject>Target 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electrochemiluminescence biosensor for the detection of soybean agglutinin based on carboxylated graphitic carbon nitride as luminophore</title><author>Zhang, Cong ; Hu, Fangxin ; Zhang, Han ; Chen, Shihong ; Yuan, Ruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-419c9e228ed5e7f3b52ba21063abdd75b6a975d64f63a83bbe4c8b36f02dc82a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Antinutrition factors</topic><topic>Biochemistry</topic><topic>Biosensors</topic><topic>Carbon</topic><topic>Carbon nitride</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>D-Galactosamine</topic><topic>Detectors</topic><topic>Electrochemiluminescence</topic><topic>Electrodes</topic><topic>Food Science</topic><topic>Glassy carbon</topic><topic>Glycoproteins</topic><topic>Gold</topic><topic>Immobilization</topic><topic>Laboratory Medicine</topic><topic>Lectins</topic><topic>Monitoring/Environmental Analysis</topic><topic>Nanoparticles</topic><topic>Nitrides</topic><topic>Research Paper</topic><topic>Side effects</topic><topic>Soybean</topic><topic>Soybeans</topic><topic>Target recognition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Cong</creatorcontrib><creatorcontrib>Hu, Fangxin</creatorcontrib><creatorcontrib>Zhang, Han</creatorcontrib><creatorcontrib>Chen, Shihong</creatorcontrib><creatorcontrib>Yuan, Ruo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems 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Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Cong</au><au>Hu, Fangxin</au><au>Zhang, Han</au><au>Chen, Shihong</au><au>Yuan, Ruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An electrochemiluminescence biosensor for the detection of soybean agglutinin based on carboxylated graphitic carbon nitride as luminophore</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>411</volume><issue>23</issue><spage>6049</spage><epage>6056</epage><pages>6049-6056</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>As an important glycoprotein of the lectin family, soybean agglutinin (SBA) is an anti-nutritional factor with considerable toxic and side effects and plays a significant role in tumor analysis. In order to achieve the sensitive detection of SBA, a sandwich-structured electrochemiluminescence (ECL) biosensor was constructed using carboxylated carbon nitride (C-g-C
3
N
4
) as luminophore and D-galactosamine (galM) as a recognition element. A glassy carbon electrode (GCE) was modified with Au nanoparticles (Au NPs) for capturing the galM via Au-N bond, and further capturing the target SBA by specific recognition between galM and SBA. In the presence of SBA, the composite C-g-C
3
N
4
-galM was immobilized onto the electrode. With the increase in the concentration of SBA, the ECL signal from C-g-C
3
N
4
increased, thus achieving a signal-on detection of SBA. The linear range of the biosensor was 1.0 ng/mL~10 μg/mL and detection limit for SBA was as low as 0.33 ng/mL. In this construction strategy, C-g-C
3
N
4
not only acted as an excellent signal probe, but also as an immobilization matrix to easily achieve a high loading of the small molecule recognition element galM. This strategy provides a simple alternative SBA detection platform.
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31280477</pmid><doi>10.1007/s00216-019-01986-w</doi><tpages>8</tpages></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2019-09, Vol.411 (23), p.6049-6056 |
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| source | SpringerNature Journals |
| subjects | Analysis Analytical Chemistry Antinutrition factors Biochemistry Biosensors Carbon Carbon nitride Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science D-Galactosamine Detectors Electrochemiluminescence Electrodes Food Science Glassy carbon Glycoproteins Gold Immobilization Laboratory Medicine Lectins Monitoring/Environmental Analysis Nanoparticles Nitrides Research Paper Side effects Soybean Soybeans Target recognition |
| title | An electrochemiluminescence biosensor for the detection of soybean agglutinin based on carboxylated graphitic carbon nitride as luminophore |
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