Biodetection and bioremediation of copper ions in environmental water samples using a temperature-controlled, dual-functional Escherichia coli cell
Although a variety of whole-cell biosensors and biosorbents have been developed for detection and removal of heavy metal contaminants, few whole cells can be applied to both monitoring and remediation of copper pollution in water. In this study, a modified plasmid was constructed by incorporating a...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2019-08, Vol.103 (16), p.6797-6807 |
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| container_title | Applied microbiology and biotechnology |
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| creator | Wang, Wu Jiang, Fengying Wu, Fei Li, Jianghui Ge, Rui Li, Jin Tan, Guoqiang Pang, Yilin Zhou, Xiaofeng Ren, Xiaojun Fan, Bingqian Lyu, Jianxin |
| description | Although a variety of whole-cell biosensors and biosorbents have been developed for detection and removal of heavy metal contaminants, few whole cells can be applied to both monitoring and remediation of copper pollution in water. In this study, a modified plasmid was constructed by incorporating a copper-sensing element and a copper-adsorbing element into a temperature-inducible plasmid, pBV220. This plasmid was subsequently transformed into an engineered
Escherichia coli
strain lacking
copA
and
cueO
. This dual-functional
E. coli
cell selectively responded to copper ions with a linear detection range of 0.01–25 μM at 37 °C and could express surface-displayed CueR when treated at 42 °C without any costly chemical inducers. The display of CueR on the cell surface specifically enhanced its copper adsorption capacity and rapidly removed copper ions from aqueous solutions. In addition, the CueR surface-displayed cells could be regenerated by adsorption-desorption cycles via pH regulation. Moreover, by simply using two different temperatures, the detection or adsorption of copper using this dual-functional whole cell was achieved without any cross-interference. Most importantly, it provided highly sensitive, accurate quantification, and effective removal of copper in real environmental water samples. Thus, this
E. coli
cell can be used for large-scale detection and remediation of copper pollutants. |
| doi_str_mv | 10.1007/s00253-019-09984-9 |
| format | Article |
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Escherichia coli
strain lacking
copA
and
cueO
. This dual-functional
E. coli
cell selectively responded to copper ions with a linear detection range of 0.01–25 μM at 37 °C and could express surface-displayed CueR when treated at 42 °C without any costly chemical inducers. The display of CueR on the cell surface specifically enhanced its copper adsorption capacity and rapidly removed copper ions from aqueous solutions. In addition, the CueR surface-displayed cells could be regenerated by adsorption-desorption cycles via pH regulation. Moreover, by simply using two different temperatures, the detection or adsorption of copper using this dual-functional whole cell was achieved without any cross-interference. Most importantly, it provided highly sensitive, accurate quantification, and effective removal of copper in real environmental water samples. Thus, this
E. coli
cell can be used for large-scale detection and remediation of copper pollutants.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-019-09984-9</identifier><identifier>PMID: 31240366</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adsorption ; Aqueous solutions ; Bacteria ; Biodegradation, Environmental ; Biomedical and Life Sciences ; Bioremediation ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Cell surface ; Contaminants ; Copper ; Copper - analysis ; Copper - metabolism ; Detectors ; E coli ; Environmental Biotechnology ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli - radiation effects ; Heavy metals ; Ions ; Life Sciences ; Metabolic Engineering - methods ; Microbial Genetics and Genomics ; Microbiology ; Organic chemistry ; Pathogens ; Plasmids ; Pollutants ; Pollution detection ; Pollution monitoring ; Remediation ; Surface chemistry ; Temperature ; Trace Elements - analysis ; Trace Elements - metabolism ; Water ; Water analysis ; Water Microbiology ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - metabolism ; Water pollution ; Water sampling</subject><ispartof>Applied microbiology and biotechnology, 2019-08, Vol.103 (16), p.6797-6807</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Applied Microbiology and Biotechnology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-b4fc5f5139312aa8c544f0713638ce1dcbe05150760ada67a6c6997b60e16deb3</citedby><cites>FETCH-LOGICAL-c513t-b4fc5f5139312aa8c544f0713638ce1dcbe05150760ada67a6c6997b60e16deb3</cites><orcidid>0000-0002-6843-7861</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-019-09984-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-019-09984-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31240366$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Wu</creatorcontrib><creatorcontrib>Jiang, Fengying</creatorcontrib><creatorcontrib>Wu, Fei</creatorcontrib><creatorcontrib>Li, Jianghui</creatorcontrib><creatorcontrib>Ge, Rui</creatorcontrib><creatorcontrib>Li, Jin</creatorcontrib><creatorcontrib>Tan, Guoqiang</creatorcontrib><creatorcontrib>Pang, Yilin</creatorcontrib><creatorcontrib>Zhou, Xiaofeng</creatorcontrib><creatorcontrib>Ren, Xiaojun</creatorcontrib><creatorcontrib>Fan, Bingqian</creatorcontrib><creatorcontrib>Lyu, Jianxin</creatorcontrib><title>Biodetection and bioremediation of copper ions in environmental water samples using a temperature-controlled, dual-functional Escherichia coli cell</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Although a variety of whole-cell biosensors and biosorbents have been developed for detection and removal of heavy metal contaminants, few whole cells can be applied to both monitoring and remediation of copper pollution in water. In this study, a modified plasmid was constructed by incorporating a copper-sensing element and a copper-adsorbing element into a temperature-inducible plasmid, pBV220. This plasmid was subsequently transformed into an engineered
Escherichia coli
strain lacking
copA
and
cueO
. This dual-functional
E. coli
cell selectively responded to copper ions with a linear detection range of 0.01–25 μM at 37 °C and could express surface-displayed CueR when treated at 42 °C without any costly chemical inducers. The display of CueR on the cell surface specifically enhanced its copper adsorption capacity and rapidly removed copper ions from aqueous solutions. In addition, the CueR surface-displayed cells could be regenerated by adsorption-desorption cycles via pH regulation. Moreover, by simply using two different temperatures, the detection or adsorption of copper using this dual-functional whole cell was achieved without any cross-interference. Most importantly, it provided highly sensitive, accurate quantification, and effective removal of copper in real environmental water samples. Thus, this
E. coli
cell can be used for large-scale detection and remediation of copper pollutants.</description><subject>Adsorption</subject><subject>Aqueous solutions</subject><subject>Bacteria</subject><subject>Biodegradation, Environmental</subject><subject>Biomedical and Life Sciences</subject><subject>Bioremediation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Cell surface</subject><subject>Contaminants</subject><subject>Copper</subject><subject>Copper - analysis</subject><subject>Copper - metabolism</subject><subject>Detectors</subject><subject>E coli</subject><subject>Environmental Biotechnology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli - radiation effects</subject><subject>Heavy metals</subject><subject>Ions</subject><subject>Life Sciences</subject><subject>Metabolic Engineering - methods</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Organic chemistry</subject><subject>Pathogens</subject><subject>Plasmids</subject><subject>Pollutants</subject><subject>Pollution detection</subject><subject>Pollution monitoring</subject><subject>Remediation</subject><subject>Surface chemistry</subject><subject>Temperature</subject><subject>Trace Elements - analysis</subject><subject>Trace Elements - metabolism</subject><subject>Water</subject><subject>Water analysis</subject><subject>Water Microbiology</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - metabolism</subject><subject>Water pollution</subject><subject>Water sampling</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9Ustu1TAQjRCIXgo_wAJZYoWEi53ETrxsqwKVKiHxWFuOM7l15djBdnh8Bz_M9N5CdSWEvLBn5pwZz8ypquecnXDGujeZsVo0lHFFmVJ9S9WDasPbpqZM8vZhtWG8E7QTqj-qnuR8wxiveykfV0cNr1vWSLmpfp25OEIBW1wMxISRDC4mmGF0ZueKE7FxWSARtDJxgUD45lIMM4RiPPluCsaymRcPmazZhS0xpMCMFFPWBNTGUFL0HsbXZFyNp9MaduWQfZHtNSRnr53BMt4RC94_rR5Nxmd4dncfV1_eXnw-f0-vPry7PD-9olbwptChnayY8KmwHWN6K9p2Yh1vZNNb4KMdgAkuWCeZGY3sjLRSqW6QDLgcYWiOq5f7vEuKX1fIRd_ENeG3sq7rVooW59Xdo7bGg3ZhiiUZO7ts9alQmL7GAog6-QcKzwizwwnA5NB_QHh1QLidEvwoW7PmrC8_fTzE1nusTTHnBJNekptN-qk507dS0HspaJSC3klBKyS9uOtuHXCdfyl_do-AZg_IGApbSPft_yftbwOPv3o</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Wang, Wu</creator><creator>Jiang, Fengying</creator><creator>Wu, Fei</creator><creator>Li, Jianghui</creator><creator>Ge, 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and bioremediation of copper ions in environmental water samples using a temperature-controlled, dual-functional Escherichia coli cell</title><author>Wang, Wu ; Jiang, Fengying ; Wu, Fei ; Li, Jianghui ; Ge, Rui ; Li, Jin ; Tan, Guoqiang ; Pang, Yilin ; Zhou, Xiaofeng ; Ren, Xiaojun ; Fan, Bingqian ; Lyu, Jianxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-b4fc5f5139312aa8c544f0713638ce1dcbe05150760ada67a6c6997b60e16deb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adsorption</topic><topic>Aqueous solutions</topic><topic>Bacteria</topic><topic>Biodegradation, Environmental</topic><topic>Biomedical and Life Sciences</topic><topic>Bioremediation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Cell surface</topic><topic>Contaminants</topic><topic>Copper</topic><topic>Copper - analysis</topic><topic>Copper - metabolism</topic><topic>Detectors</topic><topic>E coli</topic><topic>Environmental Biotechnology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli - radiation effects</topic><topic>Heavy metals</topic><topic>Ions</topic><topic>Life Sciences</topic><topic>Metabolic Engineering - methods</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Organic chemistry</topic><topic>Pathogens</topic><topic>Plasmids</topic><topic>Pollutants</topic><topic>Pollution detection</topic><topic>Pollution monitoring</topic><topic>Remediation</topic><topic>Surface chemistry</topic><topic>Temperature</topic><topic>Trace Elements - analysis</topic><topic>Trace Elements - metabolism</topic><topic>Water</topic><topic>Water analysis</topic><topic>Water Microbiology</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water Pollutants, Chemical - metabolism</topic><topic>Water pollution</topic><topic>Water sampling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Wu</creatorcontrib><creatorcontrib>Jiang, Fengying</creatorcontrib><creatorcontrib>Wu, Fei</creatorcontrib><creatorcontrib>Li, Jianghui</creatorcontrib><creatorcontrib>Ge, Rui</creatorcontrib><creatorcontrib>Li, Jin</creatorcontrib><creatorcontrib>Tan, Guoqiang</creatorcontrib><creatorcontrib>Pang, Yilin</creatorcontrib><creatorcontrib>Zhou, Xiaofeng</creatorcontrib><creatorcontrib>Ren, Xiaojun</creatorcontrib><creatorcontrib>Fan, Bingqian</creatorcontrib><creatorcontrib>Lyu, Jianxin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central 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USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Wu</au><au>Jiang, Fengying</au><au>Wu, Fei</au><au>Li, Jianghui</au><au>Ge, Rui</au><au>Li, Jin</au><au>Tan, Guoqiang</au><au>Pang, Yilin</au><au>Zhou, Xiaofeng</au><au>Ren, Xiaojun</au><au>Fan, Bingqian</au><au>Lyu, Jianxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biodetection and bioremediation of copper ions in environmental water samples using a temperature-controlled, dual-functional Escherichia coli cell</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>103</volume><issue>16</issue><spage>6797</spage><epage>6807</epage><pages>6797-6807</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>Although a variety of whole-cell biosensors and biosorbents have been developed for detection and removal of heavy metal contaminants, few whole cells can be applied to both monitoring and remediation of copper pollution in water. In this study, a modified plasmid was constructed by incorporating a copper-sensing element and a copper-adsorbing element into a temperature-inducible plasmid, pBV220. This plasmid was subsequently transformed into an engineered
Escherichia coli
strain lacking
copA
and
cueO
. This dual-functional
E. coli
cell selectively responded to copper ions with a linear detection range of 0.01–25 μM at 37 °C and could express surface-displayed CueR when treated at 42 °C without any costly chemical inducers. The display of CueR on the cell surface specifically enhanced its copper adsorption capacity and rapidly removed copper ions from aqueous solutions. In addition, the CueR surface-displayed cells could be regenerated by adsorption-desorption cycles via pH regulation. Moreover, by simply using two different temperatures, the detection or adsorption of copper using this dual-functional whole cell was achieved without any cross-interference. Most importantly, it provided highly sensitive, accurate quantification, and effective removal of copper in real environmental water samples. Thus, this
E. coli
cell can be used for large-scale detection and remediation of copper pollutants.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31240366</pmid><doi>10.1007/s00253-019-09984-9</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6843-7861</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2019-08, Vol.103 (16), p.6797-6807 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_journals_2246540127 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adsorption Aqueous solutions Bacteria Biodegradation, Environmental Biomedical and Life Sciences Bioremediation Biosensing Techniques - methods Biosensors Biotechnology Cell surface Contaminants Copper Copper - analysis Copper - metabolism Detectors E coli Environmental Biotechnology Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli - radiation effects Heavy metals Ions Life Sciences Metabolic Engineering - methods Microbial Genetics and Genomics Microbiology Organic chemistry Pathogens Plasmids Pollutants Pollution detection Pollution monitoring Remediation Surface chemistry Temperature Trace Elements - analysis Trace Elements - metabolism Water Water analysis Water Microbiology Water Pollutants, Chemical - analysis Water Pollutants, Chemical - metabolism Water pollution Water sampling |
| title | Biodetection and bioremediation of copper ions in environmental water samples using a temperature-controlled, dual-functional Escherichia coli cell |
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