Chromosomal deletions mediated by CRISPR/Cas9 in Helicoverpa armigera
Helicoverpa armigera, cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated pr...
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| Veröffentlicht in: | Insect science 2019-12, Vol.26 (6), p.1029-1036 |
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| creator | Jin, Ming‐Hui Xiao, Yu‐Tao Cheng, Ying Hu, Jie Xue, Chao‐Bin Wu, Kong‐Ming |
| description | Helicoverpa armigera, cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels for targeted mutagenesis in cotton bollworm. However, genomic deletions may be more desirable to disrupt the function of noncoding genes or regulatory sequences. By injecting two sgRNAs with Cas9 protein targeting different exons, we obtained predictable genomic deletions of several hundred bases. We achieved this type of modification with different combinations of sgRNA pairs, including HaCad and HaABCC2. Our finding indicated that CRISPR/Cas9 can be used as an efficient tool to engineer genomes with chromosomal deletion in H. armigera. |
| doi_str_mv | 10.1111/1744-7917.12570 |
| format | Article |
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Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels for targeted mutagenesis in cotton bollworm. However, genomic deletions may be more desirable to disrupt the function of noncoding genes or regulatory sequences. By injecting two sgRNAs with Cas9 protein targeting different exons, we obtained predictable genomic deletions of several hundred bases. We achieved this type of modification with different combinations of sgRNA pairs, including HaCad and HaABCC2. Our finding indicated that CRISPR/Cas9 can be used as an efficient tool to engineer genomes with chromosomal deletion in H. armigera.</description><identifier>ISSN: 1672-9609</identifier><identifier>EISSN: 1744-7917</identifier><identifier>DOI: 10.1111/1744-7917.12570</identifier><identifier>PMID: 29359508</identifier><language>eng</language><publisher>Australia: Wiley Subscription Services, Inc</publisher><subject>chromosomal deletion ; Chromosome deletion ; Cotton ; CRISPR ; CRISPR/Cas9 ; Exons ; Gene sequencing ; genome editing tool ; Genomes ; Helicoverpa armigera ; knockout ; Pests ; Proteins ; Regulatory sequences ; Ribonucleic acid ; RNA ; sgRNAs ; Site-directed mutagenesis</subject><ispartof>Insect science, 2019-12, Vol.26 (6), p.1029-1036</ispartof><rights>2018 Institute of Zoology, Chinese Academy of Sciences</rights><rights>2018 Institute of Zoology, Chinese Academy of Sciences.</rights><rights>2019 Institute of Zoology, Chinese Academy of Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3710-14bd1928e15b659559c6da070a4e03bf6f24f373ae2940eaeb2a607178d4338b3</citedby><cites>FETCH-LOGICAL-c3710-14bd1928e15b659559c6da070a4e03bf6f24f373ae2940eaeb2a607178d4338b3</cites><orcidid>0000-0003-3555-4292</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1744-7917.12570$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1744-7917.12570$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29359508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jin, Ming‐Hui</creatorcontrib><creatorcontrib>Xiao, Yu‐Tao</creatorcontrib><creatorcontrib>Cheng, Ying</creatorcontrib><creatorcontrib>Hu, Jie</creatorcontrib><creatorcontrib>Xue, Chao‐Bin</creatorcontrib><creatorcontrib>Wu, Kong‐Ming</creatorcontrib><title>Chromosomal deletions mediated by CRISPR/Cas9 in Helicoverpa armigera</title><title>Insect science</title><addtitle>Insect Sci</addtitle><description>Helicoverpa armigera, cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels for targeted mutagenesis in cotton bollworm. However, genomic deletions may be more desirable to disrupt the function of noncoding genes or regulatory sequences. By injecting two sgRNAs with Cas9 protein targeting different exons, we obtained predictable genomic deletions of several hundred bases. We achieved this type of modification with different combinations of sgRNA pairs, including HaCad and HaABCC2. Our finding indicated that CRISPR/Cas9 can be used as an efficient tool to engineer genomes with chromosomal deletion in H. armigera.</description><subject>chromosomal deletion</subject><subject>Chromosome deletion</subject><subject>Cotton</subject><subject>CRISPR</subject><subject>CRISPR/Cas9</subject><subject>Exons</subject><subject>Gene sequencing</subject><subject>genome editing tool</subject><subject>Genomes</subject><subject>Helicoverpa armigera</subject><subject>knockout</subject><subject>Pests</subject><subject>Proteins</subject><subject>Regulatory sequences</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>sgRNAs</subject><subject>Site-directed mutagenesis</subject><issn>1672-9609</issn><issn>1744-7917</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkM9PwjAUxxujEUTP3swSz4P-WrsezYJCQtSAnptue9ORjWILGP57i0Ouvktfmk-_7_WD0C3BQxJqRCTnsVREDglNJD5D_dPNeeiFpLESWPXQlfdLjJmiil6iHlUsUQlO-2icfTrbWm9b00QlNLCp7cpHLZS12UAZ5fsom08Xr_NRZryK6lU0gaYu7A7c2kTGtfUHOHONLirTeLg5ngP0_jh-yybx7OVpmj3M4oJJgmPC85IomgJJchEWSFQhSoMlNhwwyytRUV4xyQxQxTEYyKkRWBKZlpyxNGcDdN_lrp392oLf6KXdulUYqSnlLBVEJGmgRh1VOOu9g0qvXd0at9cE64M2fZCkD5L0r7bw4u6Yu83D10_8n6cAJB3wXTew_y9PT58XXfAPoAZ04Q</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Jin, Ming‐Hui</creator><creator>Xiao, Yu‐Tao</creator><creator>Cheng, Ying</creator><creator>Hu, Jie</creator><creator>Xue, Chao‐Bin</creator><creator>Wu, Kong‐Ming</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-3555-4292</orcidid></search><sort><creationdate>201912</creationdate><title>Chromosomal deletions mediated by CRISPR/Cas9 in Helicoverpa armigera</title><author>Jin, Ming‐Hui ; Xiao, Yu‐Tao ; Cheng, Ying ; Hu, Jie ; Xue, Chao‐Bin ; Wu, Kong‐Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3710-14bd1928e15b659559c6da070a4e03bf6f24f373ae2940eaeb2a607178d4338b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>chromosomal deletion</topic><topic>Chromosome deletion</topic><topic>Cotton</topic><topic>CRISPR</topic><topic>CRISPR/Cas9</topic><topic>Exons</topic><topic>Gene sequencing</topic><topic>genome editing tool</topic><topic>Genomes</topic><topic>Helicoverpa armigera</topic><topic>knockout</topic><topic>Pests</topic><topic>Proteins</topic><topic>Regulatory sequences</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>sgRNAs</topic><topic>Site-directed mutagenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Ming‐Hui</creatorcontrib><creatorcontrib>Xiao, Yu‐Tao</creatorcontrib><creatorcontrib>Cheng, Ying</creatorcontrib><creatorcontrib>Hu, Jie</creatorcontrib><creatorcontrib>Xue, Chao‐Bin</creatorcontrib><creatorcontrib>Wu, Kong‐Ming</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Insect science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, Ming‐Hui</au><au>Xiao, Yu‐Tao</au><au>Cheng, Ying</au><au>Hu, Jie</au><au>Xue, Chao‐Bin</au><au>Wu, Kong‐Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromosomal deletions mediated by CRISPR/Cas9 in Helicoverpa armigera</atitle><jtitle>Insect science</jtitle><addtitle>Insect Sci</addtitle><date>2019-12</date><risdate>2019</risdate><volume>26</volume><issue>6</issue><spage>1029</spage><epage>1036</epage><pages>1029-1036</pages><issn>1672-9609</issn><eissn>1744-7917</eissn><abstract>Helicoverpa armigera, cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels for targeted mutagenesis in cotton bollworm. However, genomic deletions may be more desirable to disrupt the function of noncoding genes or regulatory sequences. By injecting two sgRNAs with Cas9 protein targeting different exons, we obtained predictable genomic deletions of several hundred bases. We achieved this type of modification with different combinations of sgRNA pairs, including HaCad and HaABCC2. 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| subjects | chromosomal deletion Chromosome deletion Cotton CRISPR CRISPR/Cas9 Exons Gene sequencing genome editing tool Genomes Helicoverpa armigera knockout Pests Proteins Regulatory sequences Ribonucleic acid RNA sgRNAs Site-directed mutagenesis |
| title | Chromosomal deletions mediated by CRISPR/Cas9 in Helicoverpa armigera |
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